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Ectosymbionts of the Sea Anemone Stichodactyla gigantea at Kosrae, Micronesia

  • Hayes, Floyd E.;Painter, Brandon J.
    • Animal Systematics, Evolution and Diversity
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    • v.32 no.2
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    • pp.112-117
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    • 2016
  • We studied the ectosymbionts associating with the sea anemone Stichodactyla gigantea at Kosrae, Micronesia. Ectosymbionts of seven species associated with 60.7% of S. gigantea (n=28), with a mean of 2.4 per anemone and 3.9 per occupied anemone. Anemones hosting one or more ectosymbionts did not differ significantly in size from anemones lacking ectosymbionts and there was no significant correlation between anemone size and the number of ectosymbionts. Of 67 ectosymbionts observed, the sea cucumber Stichopus vastus comprised 23.9%, followed by the shrimp Thor amboinensis (20.9%), unidentified hermit crabs (Paguroidea; 20.9%), the cardinalfish Ostorhinchus novemfasciatus (20.9%), the shrimp Periclimenes brevicarpalis (9.0%), the sea cucumber Holothuria hilla (3.0%), and an unidentified brachyuran crab (1.5%). This study documents the first records of S. vastus, H. hilla, and O. novemfasciatus associating with S. gigantea, and the first locality records of S. gigantea, T. amboinensis, P. brevicarpalis, and S. vastus for Kosrae. Because humans often harvest S. gigantea for food at Kosrae, we recommend protecting the symbiotic assemblage of S. gigantea by establishing a sustainable system of harvesting.

Influence of elevated temperatures on the physiological response of hemolymph from two species of abalone, Haliotis gigantea and Haliotis discus discus (Reeve, 1846) (수온 증가에 따른 말전복, Haliotis gigantea과 둥근전복, Haliotis discus discus (Reeve, 1846) hemolymph의 생리학적 변화)

  • Min, Eun-Young;Kim, Shin-Hu;Hwang, In-Ki;Kim, Kyeong-Wook;Park, Bo-Mi;Lee, Jung Sick;Kang, Ju-Chan
    • The Korean Journal of Malacology
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    • v.31 no.3
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    • pp.187-194
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    • 2015
  • This study was conducted to examine the effects of alterations in water temperature (WT) on biochemical and immunological factors in the hemolymph of the abalones, Haliotis gigantea and H. discus discus. The abalone were exposed to various WT; 18, 20, 22, 24, 26 and $28^{\circ}C$ for 96 hours. In biochemical factors, total-protein (TP), glucose, magnesium (Mg), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were not significant changes in hemolymph of H. gigantea and H. discus discus. But calcium was significantly increased by high WT (${\geq}24^{\circ}C$). In immunological factor, The phenoloxidase (PO) activity was decreased in hemolymph of H. gigantea and H. discus discus exposed to high temperature (${\geq}22^{\circ}C$) compared to the control (P < 0.05). Whereas alkaline phosphatase (ALP) was not significantly changed. These results suggested that high temperature adversely affects the immunity of H. gigantea and H. discus discus.

Outbreak of Rust Caused by Coleosporium asterum on Solidago virgaurea var. gigantea in Ulleung-do

  • Back, Chang-Gi;Nam, Gu-Youn;Lee, Seung-Yeol;Jung, Hee-Young
    • Mycobiology
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    • v.42 no.1
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    • pp.79-81
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    • 2014
  • From June to October 2012, severe outbreaks of rust disease on Solidago virgaurea var. gigantea were observed in 5 different regions of Ulleung-do, Korea. Typical rust symptoms appeared as yellow spots, uredinia on leaves, and massive urediniospores covering the entire plant. Severely infected plants became discolored and died. On the basis of its morphological and molecular characteristics, the causal agent was identified as Coleosporium asterum. This pathogen has been reported as rust disease on S. virgaurea in the Canada and United States. In Korea, rust disease on S. virgaurea var. gigantea was reported as Uromyces rudbeckia in 1992. To the best of our knowledge, C. asterum is a novel rust pathogen on S. virgaurea var. gigantea in Korea.

Population Structure and Regeneration Status of Cyathea gigantea (Wallich ex Hook. f.) Holttum, a Tree Fern in Arunachal Pradesh, India

  • Paul, Ashish;Bhattacharjee, Sonal;Choudhury, Baharul Islam;Khan, Mohamed Latif
    • Journal of Forest and Environmental Science
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    • v.31 no.3
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    • pp.164-176
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    • 2015
  • Cyathea gigantea is a tree fern distributed throughout humid tropical regions of northeast India and other parts of the country. However, wild populations of this species are largely affected by various natural and anthropogenic activities. Therefore, an attempt was made to study the population structure and regeneration status of C. gigantea in and around greater Itanagar area of Papum Pare district, Arunachal Pradesh. Altogether 45 patches, ranging from 19.63 to $260m^2$ of area, were randomly sampled to study population structure and regeneration status of the species. Population study showed highest number of youngs in the height class of 0.50-0.75 m while, immatures were recorded highest in the height class of 2.0 to 2.5 m. Majority of the matures belonged to 6-9 m height class while it was recorded maximum in the diameter class of 10-15 cm. Average density of C. gigantea was $0.07individuals\;ha^{-1}$ which varied greatly among different patches with a range of 2 to 14. Significant correlations were found between patch size and density of youngs, immatures, matures and total density. Maximum concentration of youngs was observed in patch size $60-140m^2$, while for immatures, it was highest in patch size $20-160m^2$. Similarly, highest concentration of matures was observed in patch size $20-80m^2$ and $80-180m^2$. Population structure of the total population exhibited inverted pyramid shaped distribution. Population structure consisting of youngs, immatures, matures showed that around 60% patches lack of regenerating individuals which depict very poor natural regeneration of the species. Effective conservation strategies are therefore to be formulated to save C. gigantea from the threat of extinction in near future.

Gonadal Maturation and Main Spawning Period of Haliotis gigantea (Gastropoda: Haliotidae)

  • Shin, So Ryung;Kim, Hyeon Jin;Lee, Dong Han;Kim, Hyejin;Sohn, Young Chang;Kim, Jae Won;Lee, Jung Sick
    • Development and Reproduction
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    • v.24 no.2
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    • pp.79-88
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    • 2020
  • This study was carried out to obtain information on the developmental biology and the management of biological resources of the abalone Haliotis gigantea in Korea. The sex ratio (female:male) in the present study was 1:1.7 and the proportion of females was 36.6% (n=106/290). Their gonadal structures displayed definitive seasonal changes which were similar in pattern to the changes in the gonad index (GI). The GI showed a pattern of definitive seasonal changes in both males and females it was high in the fall and low in the spring. The reproductive cycle could be categorized into the following six stages: inactive, early active, late active, ripe, spent, and degenerative stage. Based on the monthly changes in GI and stages of gonadal development, October to November was determined to be the main spawning period for H. gigantea on Jeju Island, Korea.

Biologics For The Protection Of Forests On The Basis Of Mushroom Phlebiopsis Gigantea With Deep Cultivation On Alcohol Stillage Production

  • Kuznetsov, Ilya
    • The Korean Journal of Food & Health Convergence
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    • v.4 no.3
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    • pp.6-11
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    • 2018
  • In the Republic of Belarus as well as in the world acute problem of protecting forests from diseases and pests. The damage caused by root rot is essential, therefore, the problem of forest protection is an urgent task. The biologics has the greatest prospects in according with traditional methods of struggle. Deep method of cultivation of a mushroom Phlebiopsis gigantea with use of nutrient mediums on the basis of ethanol stillage and its components (fugat) is researched. Feasibility of use stillage as raw materials in production of a biological product for the wood protection against root decay is shown. The effect of different additives (sawdust, fodder yeast) on the accumulation of reactive biological product - oidy has been studed It was determined that the deep cultivation using sawdust of the highest accumulation oidy (1.5 $10^6units/ml$). It was also found that the stillage is the best breeding ground for fungus biomass accumulation (7.9 9.8 g / l) versus fugat (6.0 6.6 g / l). On the basis of research work the technological scheme for production of a biological product were developed. Based on the conducted studies, a technological scheme was proposed for obtaining a biological preparation by deep cultivation of the fungus Phlebiopsis gigantea.

Molecular Identification and Effects of Temperature on Survival and Growth of Hybrids between Haliotis gigantea Gmelin (♀) and Haliotis discus hannai Reeve (♂)

  • An, Hye Suck;Han, Jong Won;Hwang, Hyun-Ju;Jeon, Hancheol;Jung, Seung-Hyun;Jo, Seonmi;Choi, Tae-Young;Hyun, Young Se;Song, Ha Yeun;Whang, Ilson
    • Journal of Marine Life Science
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    • v.2 no.2
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    • pp.83-89
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    • 2017
  • In abalones, interspecific hybridization has been suggested as a possible means to increase production and desired traits for the industry. In Korea, Haliotis gigantea is considered a species with a larger size and higher temperature tolerance than H. discus hannai. However, H. discus hannai is considered the most valuable and popular fishery resource due to its better acceptance and higher market prices. Thus, viable interspecific hybrids have been produced by artificial inseminating H. gigantea eggs with H. discus hannai sperm. However, the reciprocal hybrid cross was not successful. In this study, the hybridity and the growth and thermal tolerance performance of the interspecific hybrids were examined. A combination of various assays revealed maximum growth occurrence at 21℃ and the higher growth rate in the hybrids than that of H. discus hannai parent. In addition, the growth and survival at high-temperature (28℃) of the hybrids was equivalent to that of the highly tolerant H. gigantea parent, suggesting new possibilities to overcome the mass mortality in H. discus hannai during high temperature periods of summer season in Korea. Furthermore, the induced interspecific hybrid status was confirmed by the presence of species-specific bands for each parental species of the random amplified polymorphic DNA (RAPD) profiles using universal rice primer (URP), which could be used as speciesspecific markers to distinguish the hybrids and their parental species.

The Complete Mitochondrial Genome of Dendronephthya gigantea (Anthozoa: Octocorallia: Nephtheidae)

  • Park, Eun-Ji;Kim, Bo-A;Won, Yong-Jin
    • Animal Systematics, Evolution and Diversity
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    • v.26 no.3
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    • pp.197-201
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    • 2010
  • We sequenced the whole mitochondrial genome of Dendronephthya gigantea (Anthozoa: Octocorallia: Nephteidae), the first mitochondrial genome sequence report in the Family Nephtheidae. The mitochondrial genome of D. gigantea was 18,842 bp in length, and contained 14 protein coding genes (atp6 and 8, cox1-3, cytb, nd1-6 and 4L, and msh1), two ribosomal RNAs, and only one transfer RNA. The gene content and gene order is identical to other octocorals sequenced to date. The portion of the noncoding regions is slightly larger than the other octocorals (5.08% compared to average 3.98%). We expect that the information of gene content, gene order, codon usage, noncoding region and protein coding gene sequence could be used in the further analysis of anthozoan phylogeny.

Antimicrobial and Cytotoxic Activity of Di-(2-ethylhexyl) Phthalate and Anhydrosophoradiol-3-acetate Isolated from Calotropis gigantea (Linn.) Flower

  • Habib, M. Rowshanul;Karim, M. Rezaul
    • Mycobiology
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    • v.37 no.1
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    • pp.31-36
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    • 2009
  • A phytochemical study on the flower of Calotropis gigantea (Linn.) using silica gel column chromatography and preparative thin layer chromatography, led to the first time isolation of Di-(2-ethylhexyl) phthalate (compound 1) and anhydrosophoradiol-3-acetate (compound 2). The structures of these compounds were confirmed by spectroscopic analyses (IR, HRTOFMS and NMR). The antibacterial and antifungal activities of ethyl acetate extract, compound 1 and compound 2 were measured using the disc diffusion method. Ethyl acetate extract and compound 1 presented better results than compound 2. The minimum inhibitory concentrations (MICs) of the extract and compounds were found to be in the range of $16{\sim}128{\mu}g/ml$. The cytotoxicity ($LC_{50}$) against brine shrimp nauplii (Artemia salina) were also evaluated and found to be 14.61 ${\mu}g/ml$ for ethyl acetate, 9.19 ${\mu}g/ml$ for compound 1 and 15.55 ${\mu}g/ml$ for compound 2.

Pytochemical Constituents of the Aerial Parts from Solidago virga-aurea var. gigantea

  • Choi, Sang-Zin;Choi, Sang-Un;Lee, Kang-Ro
    • Archives of Pharmacal Research
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    • v.27 no.2
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    • pp.164-168
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    • 2004
  • The chromatographic separation of the MeOH extract of the aerial parts of Solidago virga-aurea var. gigantea $M_{IQ}$ . (Compositae) led to the isolation of six terpenoids and four phenolic compounds, trans-phytol (1), ent-germacra-4(15),5,10(14 )-trien-1$\alpha$-ol (2), $\beta$-amyrin acetate (3), ent-germacra-4(15),5,10(14)-trien-1$\beta$-ol (4), $\beta$-dictyopterol (5), oleanolic acid (6), kaempferol (7), kaempferol-3-O-rutinoside (8), methyl 3,5-di-O-caffeoyl quinate (9), and 3,5-di-O-caffeoyl quinic acid (10). Their structures were established by chemical and spectroscopic methods. Compounds 4, 5, and 10 showed moderate cytotoxicity against five cultured human tumor cell lines in vitro with its E $D_{50}$ values ranging from 1.52∼18.57 $\mu\textrm{g}$/mL.L.