• Journal of environmental and Sanitary engineering
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• v.12 no.2
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• pp.59-64
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• 1997

For purification of a 70kDa toxic protein of mosquitocidal delta-endotoxin from B. thuringiensis strain H9B, immuno-affinity chromatography was performed. After separation of 70kDa toxic proteins from the delta-endotoxin of the strain H9B on SDS-PAGE, the 70kDa toxic protein was subcutaneously injected into rabbit for making a polyclonal antibody. A anti-70kDa toxic protein was purified by a column chromatography packed with protein A-sepharose 4B gels. The 70kDa toxic protein from delta-endotoxin of the strain H9B was also purified by an immuno-affinity chromatography packed with CNBr-activated sepharose 4B gels conjugated anti-70kDa toxic protein after elution with 1/10M citric acid-1/5M Na$_{2}$HPO$_{4}$ buffer(pH3.2) containing 0.5M NaCl. The 70kDa toxic protein was purified through only one step-separation system, was demonstrated by SDS-PAGE and immunoblot.

• New & Renewable Energy
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• v.3 no.2 s.10
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• pp.47-52
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• 2007

Hydrate phase equilibrium for the binary $CO_{2}$+water and $CH_{4}$+water mixtures in silica gel pore of nominal 6, 30, and 100 nm were measured and compared with the cacluated results based on van der Waals and Platteeuw model. At a specific temperature three-phase hydrate-water-vapor (HLV) equilibrium curves for pore hydrates were shifted to the higher-pressure condition depending on pore sizes when compared with those of bulk hydrates. Notably, hydrate phase equilibria for the case of 100 nominal nm pore size were nearly identical with those of bulk hydrates. The activities of water in porous silica gels were modified to account for capillary effect, and the calculation results were generally in good agreement with the experimental data.

• Korean Journal of Microbiology
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• v.29 no.3
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• pp.179-183
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• 1991

The whole proteins from 10 different Saccharomyces species were separated by isoelectric focusing, which was carried out in pH gradient polyacrylamide gels with the carrier ampholytes of various pH ranges. About 25 protein bands were found in the gel using pH 3.0-10.0 carrier ampholytes. In gel using pH 4.0-7.0 carrier ampholytes, the protein band of pI 6.3 was found in Sacch. cerevisiae NCYC 478, ATCC 26787, Sacch. rosei and Sacch. uvarum, but it was absent in Sacch. cerevisiae ATCC 24903, ATCC 42949, ATCC 36029, Sacch. steineri var hara, Sacch. bayanus, and Sacch. diastaticus.

• Journal of the Korean Ceramic Society
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• v.36 no.11
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• pp.1189-1197
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• 1999

Low-density silica gel monolith was synthesized at ambient pressure by surface modification with TMCS and sub-sequent heat treatment. The mechanical thermal and optical properties of gel were studied. Compressive strength and modulus of compression of 350$^{\circ}C$-heated gel with the density of 0.24g/cm3 were 250kPa and 0.2MPa respectively. The thermal conductivity of silica gels synthesized at ambient pressure exhibited 0.02 W/m$.$K for the density of 0.24g/cm3 which is similar to that of the gel via supercritical drying and their main thermal transfer mechanism is considered to be solid and radiation conduction at room temperature. Ambient-dried silica gels were transparent blue showing about 60% of transmittance in the wavelength of 1500-2100nm and typical absorption bands of existing bonds under heat treatment at 350$^{\circ}C$. Medium scale monolity(${\Phi}$=50mm) at ambient pressure could be successfully prepared through total 5-month process period.

• Macromolecular Research
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• v.14 no.3
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• pp.365-372
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• 2006

Poly[styrene-b-(ethylene-co-butylene)-b-styrene](SEBS) triblock copolymer was studied by dissolving the ethylene butylene midblock in selective hydrocarbon oils. These oils differ in their aromatic, paraffinic and naphthenic content. Dynamic rheological studies showed that the storage modulus (G') exceeded the loss modulus (G') for all the gels over the entire range of frequency, thereby confirming them as physical gels. However, the behavior of G' and G' as a function of frequency depended primarily on the oil type. The gelation melting temperature decreased drastically with increased oil aromaticity. Small angle X-ray scattering studies revealed that the maximum interdomain interference shifted to a higher angle depending on the composition and type of hydrocarbon oil.

• International Journal of Railway
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• v.8 no.2
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• pp.42-45
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• 2015

Alkali-silica reaction (ASR) is a chemical reaction in concrete that alkalis in cement react with reactive silica in aggregate in the presence of water. When ASR takes place, it produces gels that absorb water and expand. Swelling of ASR gels can damage concrete and cause cracking and volume expansion in concrete structure. In this paper, mechanical consequences of ASR on concrete are simulated by a finite element (FE) analysis. An FE model of concrete is built. The evolution of concrete mechanical properties subjected to ASR is achieved by FE analyses. The constitutive model of concrete is attained via the FE analysis. A case study is used to demonstrate the proposed method. The simulated results using the proposed model are in good agreement with the observations of concrete with ASR reported in the literature. The results can be used for a basic research to enhance durability of concrete slab tracks and concrete railway sleepers.

• Journal of the Korean Ceramic Society
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• v.40 no.9
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• pp.837-841
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• 2003

Duplex microstructure of zirconia and alumina has been achieved via an organic-inorganic solution technique. Zirconium 2,4-pentanedionate, aluminum nitrate and polyethylene glycol were dissolved in ethyl alcohol without any precipitation. The organicinorganic precursor gels were turned to porous powders having volume expansion through explosive, exothermic reaction during drying process. The volume expansion was caused by abrupt decomposition of the organic groups in the gels during the vigorous exothermic reaction. The volume expanded, porous powders were crystallized and densified at 1500$^{\circ}C$ for 1 h. At the optimum amount of the PEG polymer, the metal cations were well dispersed in the solution and a homogeneous polymeric network was formed. The polymer content also affected on the specific surface area of the synthesized powder and the grain size of the sintered composite.

• Journal of the Korean Ceramic Society
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• v.27 no.6
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• pp.747-754
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• 1990

Aluminum hydrate gels were prepared from the mixtures of bauxite and ammonium sulfate by wet acid process. Optimum conditions for obtaining the maximum yield( 99%) of aluminum hydrates from the same amount of bauxite were confirmed as follows ; 1. Mixing ratio ; addition of 25mole% of ammonium sulfate to 1mole of bauxite. 2. Calcination ; heated at 350℃ for 1hr. 3. Extraction ; leached at 95℃ in 1% H2SO4 for 90min. 4. pH of precipitating solution; slight below 7.0. Amorphous aluminum hydrates were precipitated at the pH lower than 8.5, but the precipitates crystallized to bayerite at the pH was 10. Mean diameter of α-Al2O3 powders which were obtained by calcining the aluminum hydrates was below 0.2㎛, and EDS analysis revealed than SiO2 was it's primary impurity.

• Journal of the Korean Ceramic Society
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• v.28 no.8
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• pp.635-639
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• 1991

Monolithic gels in the ZrO2-SiO2 system containing up to 30 mol% ZrO2 were prepared from the mixed solutions of Zr(O.nC3H7)4 and partially prehydrolyzed Si(OC2H5)4 (Tetraethyl orthosilicate) by the sol-gel method. The effect of parameters such as the hydrolysis temperature, the amount of water and HCl on the hydrolysis condensation process was investigated and the obtained gels were studied by the IR spectra and TG-DTA. The results showed that the gelation time becomes shorter with increasing content of HCl, H2O and gelation temperature, and that the polymerization was more easily completed with the higher water volume causing the elimination of unreacted organic groups.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09a
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• pp.440-441
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• 2006

Several titanate powders ($Al_2TiO_5,\;SrTiO_3$, etc.) were synthesized by an ethylene glycol solution route. Titanium isopropoxide and nitrate salts were dissolved in stoichiometric proportions in liquid-type ethylene glycol without any precipitation. The parent precursor sols were dried to porous gels, and then the gels were calcined and crystallized. All synthesized titanate powders had stable crystallization behavior at low temperature and high specific surface area after a simple ball-milling process. A three-component PZT $(Pb(Zr_{0.52}{\cdot}Ti_{0.48})O_3)$ powder was also synthesized successfully by the ethylene glycol method. In this study, the characteristics of the multi-component titanate powders by the ethylene glycol method are examined.