• BMB Reports
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• 제56권6호
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• pp.347-352
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• 2023

The protein family of poly (ADP-ribose) polymerases (PARPs) is comprised of multifunctional nuclear enzymes. Several PARP inhibitors have been developed as new anticancer drugs to combat resistance to chemotherapy. Herein, we characterized PARP4 mRNA expression profiles in cisplatin-sensitive and cisplatin-resistant ovarian cancer cell lines. PARP4 mRNA expression was significantly upregulated in cisplatin-resistant ovarian cancer cell lines, and this upregulation was associated with the hypomethylation of specific cytosine-phosphate-guanine (CpG) sites (cg18582260 and cg17117459) on its promoter. Reduced PARP4 expression was restored by treating cisplatin-sensitive cell lines with a demethylation agent, implicating the epigenetic regulation of PARP4 expression by promoter methylation. Depletion of PARP4 expression in cisplatin-resistant cell lines reduced cisplatin chemoresistance and promoted cisplatin-induced DNA fragmentation. The differential mRNA expression and DNA methylation status at specific PARP4 promoter CpG sites (cg18582260 and cg17117459) according to cisplatin responses, was further validated in primary ovarian tumor tissues. The results showed significantly increased PARP4 mRNA expressions and decreased DNA methylation levels at specific PARP4 promoter CpG sites (cg18582260 and cg17117459) in cisplatin-resistant patients. Additionally, the DNA methylation status at cg18582260 CpG sites in ovarian tumor tissues showed fairly clear discrimination between cisplatin-resistant patients and cisplatin-sensitive patients, with high accuracy (area under the curve = 0.86, P = 0.003845). Our findings suggest that the DNA methylation status of PARP4 at the specific promoter site (cg18582260) may be a useful diagnostic biomarker for predicting the response to cisplatin in ovarian cancer patients.

• Toxicological Research
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• 제22권2호
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• pp.75-85
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• 2006

This study was designed to evaluate the possible DNA damaging effects of T-2 toxin using an alkaline single cell gel electrophoresis (SCGE) comet assay and also to investigate toxic effects in chickens. A total of 20 chickens were used in these experiments. Graded concentrations of dietary T-2 toxin (0, 4, 8, and $16{\mu}g/g$ of diet) were given to groups of 5 broiler chickens. In comet assay, The DNA damage was analysed by the tail extent moment (TEM) and tail length (TL), which were used as markers of DNA strand breaks in SCGE. A significant dose-dependent increase in the extent of DNA migration as well as in the percentage of cells with tails was observed after treatment with T-2 toxin (P<0.05). Treatment with the low T-2 toxin ($4{\mu}/g$ of diet) induced a relatively low level of DNA damage in comparison with the high T-2 toxin ($16{\mu}/g$ of diet) group. The growth rate was significantly reduced by concentrations of 8, and $16{\mu}/g$ of diet (P < 0.05). The feed conversion ratio were significantly affected by any concentrations (P < 0.05). The relative weight of the spleen, and lung was decreased by the growth inhibitory concentrations. The bursa of Fabricius, thymus, and kid- ney were decreased in relative weight by concentrations of $16{\mu}/g$ of diet. The relative weight of the liver and heart were unaffected. The hemoglobin (Hb), hematocrit (HCT), and mean corpuscular hemoglobin (MCH) were decreased at concentration of $16{\mu}/g$ of diet. As compared with control chickens, there was no marked change in serum components except uric acid in T-2 treated chickens. All lymphoid tissues retained atrophic and lymphoid cell depletion throughout the three weeks trial.

• 한국식품조리과학회지
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• 제27권4호
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• pp.99-106
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• 2011

아까시 꽃 10 g에 200 mL의 두 가지 용매(물, 70% 에탄올)를 각각 가하여 추출한 다음, 농축하여 각각의 용매별 추출물을 얻었다. 이 용매별 추출물을 이용하여 아까시 꽃의 항산화 활성을 조사하였다. 그 결과, 총 페놀 함량은 물 추출물이 9.07 mg GAE/g로 70% 에탄올 추출물보다 높았고, 총 플라보노이드 함량은 70% 에탄올 추출물에서 0.04 mg CE/g, 물 추출물은 0.03 mg CE/g으로 나타났다. DPPH 라디칼 소거능은 대부분 70% 에탄올 추출물이 물 추출물 보다 더 높은 활성을 나타내었고, 환원력의 경우에는 물 추출물의 환원력은 1,000 mg/mL의 농도에서 0.438로 가장 높았으며 70% 에탄올보다 더 높은 경향을 보였다. Comet assay를 이용한 항유전독성의 효과 분석 결과, 물 추출물에서는 모든 농도에서 유의적인 DNA 손상 억제력을 보였으며, 에탄올 추출물에서는 고농도(10, 50 ${\mu}g/ml$)에서 DNA 손상이 감소되는 것을 알 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제13권2호
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• pp.236-242
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• 2003

A culture-independent and -dependent survey of the bacterial community structure in the rhizosphere and soil samples from hot pepper plants was conducted using 16S rDNA clone library and FAME analyses. Out of the 78 clones sequenced, 56% belonged to Proteobacteria, 4% to high G+C Gram- positive group, 3% to Cytophyga-Flexibacter-Bacreroides, and 32% could not be grouped with any known taxonomic division. Among the 127 FAME isolates identified, 66% belonged to low G+C Gram-positive bacteria (Baciilus spp.) and 26% to high G+C Gram-positive bacteria. In a cluster analysis, the results for both methods were found to be strikingly dissimilar. The current study is the first comparative study of FAME and 165 rDNA clonal analyses performed on the same set of soil, rhizosphere soil, and root samples.

• Natural Product Sciences
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• 제9권2호
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• pp.105-108
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• 2003

During our research program to find DNA strand-scission agents from higher plants, the MeOH extracts of the stems of Mucuna birdwoodiana Tutcher. (Leguminosae) exhibited the most potent activity with an $IC_{50}$ value of $4.9\;{\mu}g/ml$. Thus, detailed laboratory investigation was performed, and led to the isolation of known compounds, $({\pm})$-catechin (1) and (-)-epicatechin(2) as active principles. Compounds 1 and 2 showed significant activity of DNA strand-scission with $IC_{50}$ values of 10.8 and $7.5\;{\mu}g/ml$, respectively (positive control, bleomycin: $IC_{50}\;3.3\;{\mu}g/ml$.

• 공학교육연구
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• 제21권3호
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• pp.66-75
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• 2018

This paper is the second part of the January 2018 issue of the Korean Society for Engineering Education, The "Equilibrium and Unbalance Analysis of Taegeuk Pattern DNA Matrix Codes," and is an extension of the paper published in the IoT Section of the 2017 Summer Conference in Jeju. In this paper, we have reviewed the history of what is life, and 5G Mobile communication: with IoT followed by recent research on influenza RNA gene mutation and DNA mutation variants, and the insights of Watson and Crick. Inspired by a single Franklin DNA X-ray diffraction photograph, they received the Nobel Prize for the Nature publication of DNA that has three patterns and regular repeatability. Professor MoonHo Lee has solved the three patterns in Diagonal, Left to Right, and Vertical matrices in a 2x2 matrix[CU; AG] and A = T = U = 30% C = G = 20%. We also proposed DNA Watch. This is the Healthcare IoT, which is seen by the DNA Watch on the wrist, the type of Tai Chi pattern of the body, and is immediately connected to the smartphone and delivered to the doctor.

• 한국산업정보학회논문지
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• 제15권1호
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• pp.37-46
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• 2010

서픽스 트리는 데이터의 내부구조를 자세히 나타내고 선형시간 탐색이 가능한 효과적인 자료구조로서 DNA 서열분석 등에 유용하다. 그러나 서열을 서픽스 트리로 구축하는 경우 트리의 크기가 원본의 최소 30배 이상으로 커지므로 테라바이트(TB)급의 대용량 DNA 서열의 경우에 메모리상의 응용은 매우 어려운 문제점이 있다. 이에 본 논문에서는 디스크를 이용한 대용량 DNA의 서픽스 트리 응용기법을 제시한다. 이때 DNA 서열구조를 고려한 서픽스 트리 선형 탐색 특성 유지를 보장한다. 이를 검증하기 위하여 9G Byte의 유전자 단편 서열을 이용해 424G Byte의 서픽스 트리를 디스크에 구축한 다음, 임의의 질의 서열에 대해 KMP알고리즘과 비교한 결과 질의 응답시간에서 우수한 성능을 보였다.

• 한국자원식물학회지
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• 제24권2호
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• pp.130-138
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• 2011

본 연구에서는 열수 팥 추출물이 hydroxyl 라디칼에 의해 유도되는 산화적 스트레스에 미치는 영향을 알아보기 위하여 항산화활성과 DNA 및 세포의 산화적 손상 억제 효과를 조사하였다. 팥 열수 추출물의 DPPH 라디칼과 hydroxyl 라디칼의 제거능은 다소 낮았으나, $Fe^{2+}$-chelating과 과산화수소 제거효과는 높게 나타나 활성산소의 생성을 억제하는 데 효과적인 것으로 확인되었다. 또한 팥 열수 추출물의 in vitro DNA cleavage, DNA migration 및 H2AX의 인산화비 억제활성은 높은 활성을 보여주고 있어 라디칼에 의한 DNA 손상 억제에 효과적으로 작용하였다. 또한 지질과산화와 p21의 발현율을 통해 세포의 산화적 손상에 미치는 영향을 살펴보면 지질과산화 억제능과 p21의 발현율에 매우 효과적으로 작용하고 있어 라디칼에 의한 산화적 스트레스로부터 세포를 보호할 것으로 생각된다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권10호
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• pp.1493-1498
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• 2014

Epigenetic factors, such as DNA methylation status, may regulate adipogenesis and lipogenesis, thus affecting intramuscular fat (IMF) deposition in longissimus dorsi muscle (LM) of beef cattle. In Korean cattle steers, the LM consists mainly of muscle tissue. However, the LM tissue also contains IMF. We compared the gene expression levels between the IMF and muscle portions of the LM after tissue separation. Real-time polymerase chain reaction analysis showed that the mRNA levels of both adipogenic peroxisome proliferator-activated receptor gamma isoform 1 (PPARG1) and lipogenic fatty acid binding protein 4 (FABP4) were higher (p<0.01) in the IMF than in the muscle portion of the LM. We determined DNA methylation levels of regulatory regions of the PPARG1 and FABP4 genes by pyrosequencing of genomic DNA. DNA methylation levels of two of three CpG sites in the PPARG1 gene promoter region were lower (p<0.05) in the IMF than in the muscle portion of the LM. DNA methylation levels of all five CpG sites from the FABP4 gene promoter region were also lower (p<0.001) in the IMF than in the muscle portion. Thus, mRNA levels of both PPARG1 and FABP4 genes were inversely correlated with DNA methylation levels in regulatory regions of CpG sites of the corresponding gene. Our findings suggest that DNA methylation status regulates tissue-specific expression of adipogenic and lipogenic genes in the IMF and muscle portions of LM tissue in Korean cattle.

• 한국어업기술학회:학술대회논문집
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• 한국어업기술학회 2003년도 춘계 수산관련학회 공동학술대회발표요지집
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• pp.313-314
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• 2003

Antiviral DNA vaccine carrying a gene for a major antigenic viral protein have received considerable attention as a new approach in vaccine development. For fish viruses effects of DNA vaccine encoding viral G gene of infectious hematopoietic necrosis virus(IHNV) and viral hemorrhagic septicemia virus (VHSV)have been demonst.ated previously(Lapatra et al., 2001) Hirame rhabdovirus (HIRRV) causes hemorragic disease on flounder. (omitted)