• Journal of Plant Biotechnology
• /
• v.44 no.2
• /
• pp.125-134
• /
• 2017

Enhanced disease susceptibility1 (EDS1) is a regulator of basal defense responses required for resistance mediated by TIR-NBS-LRR containing R proteins. We identified three transcripts of EDS1-like genes encompassing diverse/separate expression patterns, based on the transcriptome analysis by Next Generation Sequencing (NGS) of V. flexuosa inoculated with Elsinoe ampelina. These genes were designated VfEDL1 (Vitis flexuosa Enhanced Disease Susceptibility1-like1), VfEDL2 and VfEDL3, and contained 2464, 1719 and 1599 bp, with 1791, 1227 and 1599 bp open reading frames (ORFs), encoding proteins of 596, 408 and 532 amino acids, respectively. The predicted amino acid sequences of all three genes showed the L-family lipase-like domain (class 3 lipase domain), and exhibited a potential lipase catalytic triad, aspartic acid, histidine and serine in the conserved G-X-S-X-G. All three VfEDL genes were upregulated at 1 hpi against the bacterial and fungal pathogens Rizhobiumvitis and E. ampelina, respectively, except VfEDL1, which was downregulated against E. ampelina at all time points. Against E. ampelina, VfEDL2 and VfEDL3 showed downregulated expression at later time points. When evaluated against R. vitis, VfEDL1 showed downregulated expression at all time points after 1 hpi, while VfEDL3 showed upregulation up to 24 hpi. Based on the expression response, all three genes may be involved in plant resistant responses against R. vitis, and VfEDL2 and VfEDL3 show additional resistant responses against E. ampelina infection.

• Journal of Korea Multimedia Society
• /
• v.15 no.8
• /
• pp.977-985
• /
• 2012

The purpose of this study was to present a non-photorealistic rendering technique that is efficient in single images and moving images. In case of single images, they could be processed in a real-time base by realizing flow-based DoG filter and bilateral filter, which have been frequently used in the single image NPR technique recently, in the CUDA environment. In case of moving images, the investigator presented not the existing method of NPR moving images which generating images by applying the single image NPR technique to every frame, but the method of using the single image NPR technique in the first frame and stylizing it, and then of using the motion vector-based pixel mapping in the second frame on and copying the bright values of pixels that move on the frame into the location of next frame's motion vector, thus reducing unnecessary volume of calculation and maintaining the consistency between frames. In this study, the performance of this method was proved via an experiment.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.4
• /
• pp.545-555
• /
• 2014

It has been previously demonstrated that laccases exhibit great potential for use in several industrial and environmental applications. In this paper, two laccase isoenzyme genes, lccB and lccC, were cloned and expressed in Pichia pastoris GS115. The sequence analysis indicated that the lccB and lccC genes consisted of 1,563 and 1,584 bp, and their open reading frames encoded 520 and 527 amino acids, respectively. They had 72.7% degree of identity in nucleotides and 86.7% in amino acids. The expression levels of LccB and LccC were up to 32,479 and 34,231 U/l, respectively. The recombinant laccases were purified by ultrafiltration and $(NH_4)_2SO_4$ precipitation, showing a single band on SDS-PAGE, which had a molecular mass of 58 kDa. The optimal pH and temperature for LccB were 2.0 and $55^{\circ}C$ with 2,2'-azinobis-[ 3-ethylbenzthiazolinesulfonic acid (ABTS) as a substrate, whereas LccC exhibited optimal pH and temperature at 3.0 and $60^{\circ}C$. The apparent kinetic parameters of LccB were 0.43 mM for ABTS with a $V_{max}$ value of 51.28 U/mg, and the Km and $V_{max}$ values for LccC were 0.29 mM and 62.89 U/mg. The recombinant laccases were able to decolorize five types of dyes. Acid Violet 43 (100 g/ml) was completely decolorized by LccB or LccC (2 U/ml), and the decolorization of Reactive Blue KN-R (100 g/ml) was 91.6% by LccC (2 U/ml). Thus, the study characterizes useful laccase isoenzymes from T. versicolor that have the capability of being incorporated into the treatment of similar azo and anthraquinone dyes from dyeing industries.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.39 no.5
• /
• pp.477-485
• /
• 2002

In this paper, we present an efficient hardware architecture for the H.263 video codec and its VLSI implementation. This architecture is based on the unified interface by which internal hardware engines and an internal RISC processor are connected one another. The unified interface enables the modular design of internal blocks, efficient hardware/software partitioning, and pipelined paralled operations. The developed VLSI supports the H.263 version 2 profile 3 @ level 10, and moreover, both the control protocol H.245 and the multiplexing protocol H.223. Therefore, it can be used for the complete ITU-T H.324 or 3GPP 3G 324M multimedia processor with the help of an external audio codec. Simultaneous encoding and decoding of QCIF format images at a rate greater than 15 frames per second is achieved at 40 MHz clock frequency.

• Korean Journal of Microbiology
• /
• v.54 no.3
• /
• pp.302-304
• /
• 2018

We, for the first time, isolated and identified a Vibrio splendidus KCTC 11899BP producing hyaluronate lyase from seawater. This enzyme is produced only when hyaluronic acid (HA) is added to the basal medium. Hyaluronate lyases are produced by microorganisms, which degrade the ${\beta}$-(1, 4) bond of HA to produce disaccharide. The genome of KCTC 11899BP, which consist of two circular contigs that are 3,522 kb (contig 1) long and 1,986 kb (contig 2) long respectively, as like other Vibrio sp. that contained 2 chromosomes. The genome included 4,700 predicted open reading frames, G + C content 44.12%, 137 tRNA genes, and 46 rRNA genes.

• Journal of Intelligence and Information Systems
• /
• v.15 no.2
• /
• pp.33-48
• /
• 2009

Capsule endoscopy is one of the most remarkable inventions in last ten years. Causing less pain for patients, diagnosis for entire digestive system has been considered as a most convenience method over a normal endoscope. However, it is known that the diagnosis process typically requires very long inspection time for clinical experts because of considerably many duplicate images of same areas in human digestive system due to uncontrollable movement of a capsule endoscope. In this paper, we propose a method for clinical diagnosticians to get highly valuable information from capsule-endoscopy video. Our software system consists of three global maps, such as movement map, characteristic map, and brightness map, in temporal domain for entire sequence of the input video. The movement map can be used for effectively removing duplicated adjacent images. The characteristic and brightness maps provide frame content analyses that can be quickly used for segmenting regions or locating some features(such as blood) in the stream. Our experiments show the results of four patients having different health conditions. The result maps clearly capture the movements and characteristics from the image frames. Our method may help the diagnosticians quickly search the locations of lesion, bleeding, or some other interesting areas.

• Nuclear Medicine and Molecular Imaging
• /
• v.42 no.4
• /
• pp.275-284
• /
• 2008

Purpose: We intended to evaluate myocardial oxygen consumption ($MVO_2)$ by applying recirculation correction and modified one-compartment model to have a reference range of $MVO_2$ in normal young population and to reveal the effect of recirculation on time-activity curve (TAC). Materials and Methods: In nine normal male volunteers with mean age of $26.3{\pm}4.0$, $MVO_2$ was estimated with 925 MBq (25mCi) of $^{11}C$-Acetate (Neuroscience Research Institute, Gachon University of Medicine and Science, Incheon, Korea) and PET/CT (Biograph 6, Siemens Medical Solution, Germany). Analysis software such as $MATLAB^{(R)}$ v7.1 (Mathworks, Inc., United States), $Excel^{(R)}$ 2007 (Microsoft, United States), and $SPSS^{(R)}$ v12.0 (Apache Software Foundation, United States) were used. Twenty three frames were of $12{\times}10$, $5{\times}60$, $3{\times}120$, $2{\times}300's$ duration, respectively. The modified one-compartmental model and the recirculation correction method were applied. Statistical analysis was performed by using Test of Normality, ANOVA and Post-Hoc (Scheffe's) analysis, and p-value less than 0.05 was considered as significant. Results: The normal reference ranges of $MVO_2$ were presented as $3.18-4.64\;{\times}\;10^{-4}\;ml/g/sec$, $1.91-3.94\;{\times}\;10^{-4}\;ml/g/sec$, $4.31-6.40\;{\times}\;10^{-4}\;ml/g/sec$, $2.84-4.53\;{\times}\;10^{-4}\;ml/g/sec$ and $3.42-5.00\;{\times}\;10^{-4}\;ml/g/sec$ in the septum, the inferior wall, the lateral wall, the anterior wall and the entire wall, respectively. In addition, it was noted that the dual exponentiality of the clearance curve is due to the recirculation effect and that the characteristic of the curve is essentially mono-exponential. Conclusion: $^{11}C$-Acetate is a radiotracer worthwhile to assess $MVO_2$. Re-circulated $^{11}C$ can influence TAC of $^{11}C$ in myocadia and so the recirculation correction must be considered when measuring $MVO_2$.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2018.05a
• /
• pp.588-591
• /
• 2018

Baculovirus was originally isolated from the alfalfa looper and contains a 134-kbp genome with 154 open reading frames (ORF). The major capsid protein VP39 together with some minor proteins forms the nucleocapsid ($21nm{\times}260nm$) that encloses the DNA with p6.9 protein. They are double-stranded, circular, supercoiled DNA molecules in a rod-shaped capsid. Wild-type baculoviruses exhibit both lytic and occluded life cycles that develop independently throughout the three phases of virus replication. Recombinant baculoviruses can transfer their vectors and express their recombinant proteins in a wide range of mammalian cell types. Especially, inclusion of a dominant selectable marker in these baculoviral vectors can express diverse recombinant genes in many cells. Baculoviral vectors were reconstructed with cytomegalovirus (CMV) promoter,uroplakin II promoter, polyhedron promoter, vesicular stomatitis virus G (VSVG), enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) gene and so on. These reconstructed vectors were infected into various cell and cell lines. We performed transfection and expression of these recombinant vectors comparison with other control vectors. From this study, we knew that transfection and expression of these recombinant vectors have higher efficacy than any control vector. This work was supported by a grant from Mid-Career Researcher Program(NRF-2016R1A2B4016552) through the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future Planning(MSIP).

• Journal of IKEEE
• /
• v.5 no.1 s.8
• /
• pp.8-15
• /
• 2001

This paper remarks IETF based requirements for IP/UDP/RTP header compression issued in 3GPP2 All IP Ad Hoc Meeting and protocol stacks of the next generation mobile station. All IP Network, for real time application such as Voice over IP (VoIP) multimedia services based on 3GPP2 3G cdma2000. Frames for various protocols expected in the All IP network Mobile Station (MS) are explained with several figures including the bit-for-bit notation of header format based on IETF draft of Robust Header Compression Working Group (ROHC). Especially, this paper includes problems of IS-707 Radio Link Protocol (RLP) for header compression which will be expected to modify in All IP network MS's medium access layer to accommodate real time packet data service[1]. And also, since PPP has also many problems in header compression and mobility aspects in MS protocol stacks for 3G cdma2000 packet data network based on Mobile IP (PN-4286)[2], we introduce the problem of solution for header compression of PPP. Finally. we suggest the guidelines for All IP network MS header compression about expected protocol stacks, radio resource efficiency and performance.

• Korean small business review
• /
• v.42 no.1
• /
• pp.43-56
• /
• 2020

The technology conflict between the U.S. and China is deepening recently. The U.S.-China battle began as a national security issue but is comprehending as a U.S.'s check for China's rapid technological advancement. China is rapidly growing in several indexes including R&D expenditure, patent application, and publications, and is challenging the U.S. in 5G and Artificial Intelligence. In 2018, Huawei became the largest 5G network/equipment provider and second largest smart phone manufacturer in the world. Now, Huawei is outperforming at AI chipset manufacturing, Bigdata analysis and cloud, positioning to become a critical player in the 4th industrial revolution. The purpose of this research is to analyze the effect of recent Huawei issues to Korean SMEs focusing on the relation between Huawei and Korean companies; the cooperation status from the Global Value Chain (GVC) perpsective, and Korean government's policies related to Huawei's information security issues will be the three main frames for the analysis. Then, this research proposes policy implications such as increasing Korea's competitiveness in manufacturing and information security.