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The Effects of Transcription / Translation Inhibitors on Meiotic Maturation of Porcine Oocyte In Vitro

  • Byun, Tae-Ho;Lee, Sung-Ho;Park, Chang-Sik;Lee, Sang-Ho
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.117-117
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    • 2002
  • The oocytes from most of animal species accumulate genetic information and other necessary materials during oogenesis for the later use in the early development. Over the years oocyte maturation has been studied extensively both in vitro and in vivo. Particularly, maturation of follicular oocyte in vitro becomes one of the important tools for the studies of basic cell biology, the in vitro technology of animal production, and in particular, the somatic cell cloning by nuclear transfer. We examined meiotic maturation and cumulus expansion in the presence of translation or transcription inhibitors for varying periods of in viかo maturation (IVM) of pig oocyte. In Experiment 1, the results revealed that translation and transcription inhibitors inhibited cumulus expansion and meiotic maturation during 35h of IVM. However, 50 to 60% of the oocytes underwent nuclear maturation without cumulus expansion during 75h of IVM. The rest of the oocytes were arrested at metaphase I (40-50%) in the presence of the inhibitors. In Experiment II, the OCCs were exposed to the drugs only for 15h to examine translation and transcription inhibitors on cumulus expansion and meiotic maturation. Transcription inhibitors for 15h did not arrest meiotic maturation when the oocytes were cultured for subsequent, necessary period of IVM, whereas cumulus expansion was completely inhibited, suggesting that initial 15h is critical transcription activity far cumulus expansion. Translation inhibitors for 15h exposure did not alter cumulus expansion and meiotic maturation during subsequent culture in the absence of the drugs. In Experiment III, the OCCs were exposed to the drugs only for later 30h to examine the influence of transcription and translation inhibitors on oocyte maturation. Interestingly, all meiotic maturation underwent normally with full expansion of cumulus. Similar results were obtained from Experiment IV where 5h of exposure from 15 to 20h of IVM culture to the drugs was performed and subsequently cultured for same period in fresh medium. Taken there results together, both transcription and translation are necessary for nuclear maturation and cumulus expansion, and first 15h IVM for cumulus expansion is critical. The arrested oocytes by the drugs were still capable of undergoing nuclear maturation, although cumulus expansion was affected.

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Analysis of germinating seed stage expressed sequence tags in Oryza sativa L. (벼 발아종자 발현유전자의 발현특성분석)

  • Yoon, Ung-Han;Lee, Gang-Seob;Kim, Chang-Kug;Lee, Jung-Sook;Hahn, Jang-Ho;Yun, Doh-Won;Ji, Hyeon-So;Lee, Tae-Ho;Lee, Jeong-Hwa;Park, Sung-Han;Kim, Gun-Wook;Seo, Mi-Suk;Kim, Yong-Hwan
    • Journal of Plant Biotechnology
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    • v.36 no.3
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    • pp.281-288
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    • 2009
  • Seed germination is the important stage to express many genes for regulation of energy metabolism, starch degradation and cell division from seed dormancy state. For the functional analysis of seed germination mechanisms, we were analyzed the rice cDNA clones (Oryzasativa cultivar Ilpum) obtained from seed imbibition during 48 hours. Total number of 18,101 Expressed Sequence Tags (ESTs) were clustered using SeqMan program. Among them, 8,836 clones were identified as unique clones. We identified the chitinase gene specifically expressed in seed germination and amylase gene involved to starch degradation from the full length cDNA analysis, and several genes were registered to NCBI GeneBank. To analyzed the commonly expressed genes between inmature seed and germinated seed, 25,66 inmature ESTs and 18,101 germinated ESTs were clustered using SeqMan program and identified 2,514 clones as commonly expressed unigene. Among them, alpha-glubulin and alcohol dehydrogenase I were supposed to LEA genes only expressed in the immature and germinated seed stages. For the clustering of orthologous group genes, we further analyzed the 8,836 EST clones from germinating seeds using NCBI clusters of orthologous groups database. Among the clones, 5,076 clones were categorized into information storage and processing, cellular processes and signaling, metabolism and poorly characterized genes, proportioning 783 (14.29%), 1,484 (27%), 1,363 (24.8%) and 1,869 (34%) clones to the previous four categories, respectively.

A Study on the Dietary Behaviors of Female Baby Boomers and the Needs for Future Perspectives of Dietary Life (여성 베이비부머들의 식생활 태도와 미래 식생활 요구도 조사)

  • Nam, Haewon;Myung, Choonok;Park, Youngsim
    • The Korean Journal of Food And Nutrition
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    • v.26 no.4
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    • pp.895-908
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    • 2013
  • The purpose of this study is to examine female baby boomers' dietary habits and their attitudes together with their needs for future perspectives of dietary life. Our aim is to use these findings as a basic data when forecasting for food-related industries or policy making. A survey is being carried out for a total of 358 female baby boomers and analyzed by SPSS 12.0. The following is a summary of this study. The average age is 52.6 years old, most of them graduated from highschool (63.1%) and had a nuclear type of family (76.1%). Only 39.0% is composed of housewives, others had either full-time or part-time jobs. Self-assessment of stress is not so high and only 8.1% are dissatisfied with their lives. 38.2% are either overweight or obese in terms of BMI, and most of them are non-smokers (97.2%) or non-drinkers (63.0%). Their mean dietary habit scores are $70.6{\pm}11.8$, and the scores show significant relations with their education levels (p<0.01), monthly income (p<0.01), life satisfaction rates (p<0.001), stress levels (p<0.001), smoking habits (p<0.05), drinking habits (p<0.05), regular exercises (p<0.001) and regular health check-ups (p<0.05). The rate of skipping breakfast, lunch and dinner are 18.2%, 1.1%, 5.2% respectively. The main reason for skipping breakfast is the 'lack of time'. With regards to the frequency of grocery shopping, almost half of the subjects (55.7%) said '1~2 times per week' and bought mainly raw food sources such as vegetables, fruits, and meats. The majority of the subjects (91.3%) report that they cooked meals at homes, and took about 1 hour of time. The subjects also point out that cooking was a bothering task, and only 46.4% would prepare meals at home, while others would rather eat out or eat convenience foods. The main reasons for not wanting meal services in the elderly welfare facility are because they didn't want to live such places (48.4%) and the meals are tasteless (31.3%). As for delivery meal services, 60.1% are aware of it, and 39.9% would consider using it in the future. Factors to be considered when using the delivery meal service are sanitation (43.7%), nutrition (28.7%), taste (18.4%), price (6.3%), and brand name (2.9%). This study is expected to be used as useful information when developing food-related strategies for baby boomers in the future.

Projective Reconstruction Method for 3D modeling from Un-calibrated Image Sequence (비교정 영상 시퀀스로부터 3차원 모델링을 위한 프로젝티브 재구성 방법)

  • Hong Hyun-Ki;Jung Yoon-Yong;Hwang Yong-Ho
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.42 no.2 s.302
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    • pp.113-120
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    • 2005
  • 3D reconstruction of a scene structure from un-calibrated image sequences has been long one of the central problems in computer vision. For 3D reconstruction in Euclidean space, projective reconstruction, which is classified into the merging method and the factorization, is needed as a preceding step. By calculating all camera projection matrices and structures at the same time, the factorization method suffers less from dia and error accumulation than the merging. However, the factorization is hard to analyze precisely long sequences because it is based on the assumption that all correspondences must remain in all views from the first frame to the last. This paper presents a new projective reconstruction method for recovery of 3D structure over long sequences. We break a full sequence into sub-sequences based on a quantitative measure considering the number of matching points between frames, the homography error, and the distribution of matching points on the frame. All of the projective reconstructions of sub-sequences are registered into the same coordinate frame for a complete description of the scene. no experimental results showed that the proposed method can recover more precise 3D structure than the merging method.

Adaptive Selection of MIPv6 and Hierarchical MIPv6 for Minimizing Signaling Cost (시그널링 비용의 최소화를 위한 MIPv6와 계층적 MIPv6의 적응적 선택)

  • Kim Young-Hyun;Mun Young-Song
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.43 no.1 s.343
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    • pp.103-110
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    • 2006
  • Internet engineering task force (IETF) has proposed hierarchical mobile IPv6 (HMIPv6) in order to reduce a frequent location registration of a mobile node in mobile IPv6 (MIPv6). All traffics toward a mobile node must be transmitted through a MAP in HMIPv6. This brings unnecessary packet latency because of the increased processing cost of packet at the MAP. At this point the processing cost of packet at the MAP is influenced by the packet arrival rate for a mobile node and the number of mobile nodes in MAP domain. In this paper, we propose that MIPv6 and HMIPv6 are adaptively selected to minimize signaling coast of network as complementing weak point of MIPv6 and HMIPv6. After suppose that the packet arrival rate for a mobile node is fixed ,with this in mind, we find the optimal number of mobile nodes compared the total cost of HMIPv6 with the total cost of MIPv6. And if Mobile Nodes that the MAP is able to manage is full in MAP domain, a mobile node entering MAP domain is provided connection by MIPv6 instead of HMIPv6. In the conclusion, the proposed method of this paper shows that the weak points of MIPv6 and HMIPv6 are removed by adaptive selecting each other.

Design of an 1.8V 6-bit 2GSPS CMOS ADC with an One-Zero Detecting Encoder and Buffered Reference (One-Zero 감지기와 버퍼드 기준 저항열을 가진 1.8V 6-bit 2GSPS CMOS ADC 설계)

  • Park Yu Jin;Hwang Sang Hoon;Song Min Kyu
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.42 no.6 s.336
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    • pp.1-8
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    • 2005
  • In this paper, CMOS A/D converter with 6bit 2GSPS Nyquist input at 1.8V is designed. In order to obtain the resolution of 6bit and the character of high-speed operation, we present an Interpolation type architecture. In order to overcome the problems of high speed operation, a novel One-zero Detecting Encoder, a circuit to reduce the Reference Fluctuation, an Averaging Resistor and a Track & Hold, a novel Buffered Reference for the improved SNR are proposed. The proposed ADC is based on 0.18um 1-poly 3-metal N-well CMOS technology, and it consumes 145mW at 1.8V power supply and occupies chip area of 977um $\times$ 1040um. Experimental result show that SNDR is 36.25 dB when sampling frequency is 2GHz and INL/DNL is $\pm$0.5LSB at static performance.

Optical security system using multi-phase separation and phase-wrapping method (다중 위상 분할과 위상 랩핑 방법을 이용한 광 암호화 시스템)

  • Shin Chang Mok;Kim Soo Joong;Seo Dong Hoan
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.42 no.6 s.336
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    • pp.31-38
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    • 2005
  • In this paper, we proposed an optical security system based on a gray-image exclusive-OR encryption using multi-phase separation and phase-wrapping method. For encryption, a gray image is sliced into binary images, which have the same pixel value, and these images are encrypted by modified XOR rules with binary random images. The XORed images and the binary images respectively combined and converted into full phase images, called an encrypted image and a key image. For decryption, when the encrypted image and key image are used as inputs on optical elements, Practically due to limited controllability of phase range in optical elements, the original gray image cannot be efficiently reconstructed by these optical elements. Therefore, by decreasing the phase ranges of the encrypted image and key image using a phase-wrapping method and separating these images into low-level phase images using multi-phase separation, the gray image can be reconstructed by optical elements which have limited control range. The decrytion process is simply implemented by interfering a multiplication result of encrypted image and key image with reference light. The validity of proposed scheme is verified and the effects, which are caused by phase limitation in decryption process, is analyzed by using computer simulations.

Effects of The Substrate Temperature and The Thin film Thickness on The Properties of The Ga-doped ZnO Thin Film (기판온도 및 박막두께가 Ga-doped ZnO 박막의 특성에 미치는 영향)

  • Cho, Won-Jun;Kang, Seong-Jun;Yoon, Yung-Sup
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.47 no.1
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    • pp.6-13
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    • 2010
  • In this study, Ga-doped ZnO (GZO) thin films have been fabricated on Eagle 2000 glass substrates at various substrate temperatures $100{\sim}400^{\circ}C$ and thin film thickness by RF magnetron sputtering in order to investigate the structural, electrical, and optical properties of the GZO thin films. It is observed that all the thin films exhibit c-axis orientation and a (002) diffraction peak only. The GZO thin films, which were deposited at $T=300^{\circ}C$ and 400 nm, shows the highest (002) orientation, and the full width at half maximum (FWHM) of the (002) diffraction peak is $0.4^{\circ}$. AFM analysis shows that the formation of relatively smooth thin films are obtained. The lowest resistivity ($8.01{\times}10^{-4}\;{\Omega}cm$) and the highest carrier concentration ($3.59{\times}10^{20}\;cm^{-3}$) are obtained in the GZO thin films deposited at $T=300^{\circ}C$ and 400 nm. The optical transmittance in the visible region is approximately 80 %, regardless of process conditions. The optical band-gap shows the slight blue-shift with increase in doping which can be explained by the Burstein-Moss effect.

Optical Properties of ZnO Thin Films deposited by Pulsed Laser Deposition (PLD 법을 이용해 제작한 ZnO 박막의 광학적 특성)

  • Kang, Seong-Jun;Joung, Yang-Hee;Yoon, Yung-Sup
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.44 no.5
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    • pp.15-20
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    • 2007
  • We fabricated ZnO thin films on quartz substrate using pulsed laser deposition method and investigated structural and optical properties of ZnO thin films with various substrate temperatures. Regardless of the substrate temperature variation, all ZnO thin films had grown to (002) and the thin film deposited at 400 $^{\circ}C$ exhibited an excellent crystallinity having 0.24$^{\circ}$ of Full-Width-Half-Maximum (FWHM). In the result of photoluminescence property, UV and deep-level emission peaks were observed in all ZnO films and the emission peaks were changed with various substrate temperatures. An highest UV emission was exhibited on the specimen deposited at 400 $^{\circ}C$ and the FWHM of UV peak was 14 nm. The optical transmittance was about 85 % in visible region regardless of the substrate temperature. The comparison result of the bandgap energies obtained from optical transmittance and UV emission centers, the two values were about the same. From these results, it is found that UV emission center has close relationship with near band edge emission of ZnO thin film.

High Noise Margin LVDS I/O Circuits for Highly Parallel I/O Environments (다수의 병렬 입.출력 환경을 위한 높은 노이즈 마진을 갖는 LVDS I/O 회로)

  • Kim, Dong-Gu;Kim, Sam-Dong;Hwang, In-Seok
    • Journal of the Institute of Electronics Engineers of Korea SC
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    • v.44 no.1
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    • pp.85-93
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    • 2007
  • This paper presents new LVDS I/O circuits with a high noise margin for use in highly parallel I/O environments. The proposed LVDS I/O includes transmitter and receiver parts. The transmitter circuits consist of a differential phase splitter and a output stage with common mode feedback(CMFB). The differential phase splitter generates a pair of differential signals which have a balanced duty cycle and $180^{\circ}$ phase difference over a wide supply voltage variation due to SSO(simultaneous switching output) noises. The CMFB output stage produces the required constant output current and maintains the required VCM(common mode voltage) within ${\pm}$0.1V tolerance without external circuits in a SSO environment. The proposed receiver circuits in this paper utilizes a three-stage structure(single-ended differential amp., common source amp., output stage) to accurately receive high-speed signals. The receiver part employs a very wide common mode input range differential amplifier(VCDA). As a result, the receiver improves the immunities for the common mode noise and for the supply voltage difference, represented by Vgdp, between the transmitter and receiver sides. Also, the receiver produces a rail-to-rail, full swing output voltage with a balanced duty cycle(50% ${\pm}$ 3%) without external circuits in a SSO environment, which enables correct data recovery. The proposed LVDS I/O circuits have been designed and simulated with 0.18um TSMC library using H-SPICE.