• Research in Plant Disease
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• v.25 no.2
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• pp.84-88
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• 2019

A variety of microorganisms in rhizosphere affect plant health by plant growth promotion, mitigation of abiotic stresses as well as protection from pathogen attacks. In our previous study, we selected a bacterium, Bacillus mesonae H20-5, for alleviation of salinity stress in tomato plants. In this study, we verified the effect of a liquid formulation of B. mesonae H20-5 (TP-H20-5) on fruit production and phytochemical accumulation including lycopene and polyphenol in cherry tomato and strawberry fruits in on-farm tests of protected cultivation under salinity stress. When vegetables including tomato, cherry tomato, strawberry, and cucumber were treated with TP-H20-5 by irrigated systems, final marketable yields were increased by 21.4% (cherry tomato), 9.3% (ripen tomato), 120.6% (strawberry), and 14.5% (cucumber) compared to untreated control. Moreover, treatment of TP-H20-5 was showed increase of phytochemicals such as lycopene and total polyphenol compared to untreated control in cherry tomato and strawberry. Therefore, these results indicated that a formulant of B. mesonae H20-5 can be used as a potential biofertilizer for increasing fruit production and quality.

• Mycobiology
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• v.49 no.2
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• pp.173-182
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• 2021

Recently, Cryptococcus pseudolongus has been reported as a new pathogen of shiitake (Lentinula edodes). However, its pathological properties are not much known. To further understand its impact on the mushroom, we investigated the pathogen's interactions with the mycelium of shiitake, histopathological properties, host range, and sensitivity to diverse antifungal agents. The strain C. pseudolongus DUCC 4014 inhibited the mycelial growth of L. edodes strain (cultivar Sanjo 701ho) and caused browning in the mycelia confronted with the yeast on PDA. Spray inoculation of the yeast caused an abnormal browning symptom on the cap and/or gills of three shiitake cultivars grown on sawdust media in vinyl bags. Scanning electron microscopic images of the abnormally browned parts of shiitake fruit body illustrated that mushroom tissues were loosed and dispersed in the middle and edge of the cap and the arrangement of basidiospores borne on basidia in the gills was disturbed compared to those of normal shiitake fruit body. Spray inoculation also led to developing abnormal browning on the harvested fruit body, indicating C. pseudolongus could be a problem during mushroom storage. But the yeast was not able to induce abnormal browning on mushrooms of Pleurotus ferulae, Pleurotus fostreatus, and Agaricus bisporus. But it induced browning only on button mushroom (A. bisporus) when they were inoculated after wounding. Tests with 16 kinds of fungicides revealed that the cell growth of C. pseudolongus could be inhibited by benzalkonium chloride at MIC 7 ㎍/ml and benomyl at MIC 3 ㎍/ml.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.4
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• pp.340-347
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• 2015

This study was conducted to analyze the relationship between climate factors and fruit characteristics of 'Sangjudungsi' persimmon (Diospyros kaki THUNB) has been mainly cultivated in Sangju of Korea. We has been surveyed the yield and fruit characteristics such as fruit weight and soluble solids of 'Sangjudungsi' persimmon from 2010 to 2015 for five years. Also, as major meteorological factor, mean air temperature, cumulative temperature and days, the data of sunshine duration, diurnal range, and rainfall were collected from Sangju Regional Meteorological Administration. As result of that, the annual variation of fruit weight was affected by sunshine duration for April and October related starting point of fruit weight increase. The content of soluble solids was affected by sunshine duration for April and October similarly with fruit weight and was negatively correlated with the number of days with precipitation in the year. These results indicate the characteristics related to fruit quality like the above were affected by the sunshine duration and precipitation which is critical factor for the intensity of radiation during rapid growth period (April and October). Fruit number and yield per tree was commonly affected by mean air temperature of April and cumulative temperature days of October. These results also indicate that temperature for period of rapid growth stage of persimmon may have an important role in the fruit number and yield per tree.

• Journal of Bio-Environment Control
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• v.3 no.1
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• pp.52-57
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• 1994

The external qualities of cherry tomato fruits(Lycopersicon esculentum) grown in circulating deep flow hydroponic culture were non - destructively estimated in 1992 under warm climate conditions and evaluated in 1993-1994 under cool season. The fruit size of ‘Minicarol’ was by far the smallest compared to ‘Chelseamini’ and ‘Popo’. There were high correlations between external qualities of fruits-lengthwise growth harmonized with widthwise growth. The volume and/or weight of fruit could be non -destructively estimated by length and width of fruit. Volume=0.071$\times$ (length+width)$^3$＋0.451, Fresh weight=0.072$\times$ (length+width)$^3$＋0.542. These models were accurately evaluated by fruits harvested in 1994.

• Mycobiology
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• v.38 no.2
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• pp.113-117
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• 2010

Trichoderma strains were evaluated under field conditions to assay their efficacy in suppressing Alternaria fruit rot disease and promoting chili plant growth. The experiment was conducted at the Botanical Garden, Rajshahi University, Bangladesh from July 2006 to March 2007. Application of Trichoderma harzianum IMI 392432 significantly (p = 0.05) suppressed the disease compared to Alternaria tenuis (T2) treatment and improved both growth and yield. The treatment T4 (T. harzianum IMI-392432 + A. tenuis) was most effective in reducing disease percentage (72.27%) compared to A. tenuis (T1) treatment. The highest seed germination rate (85.56%) and the highest growth and yield (12.5 g/plant) was also recorded in the same treatment (T4), followed by T5 (T. harzianum IMI-392433 + A. tenuis), T6 (T. harzianum IMI-392434 + A. tenuis), T2 (T. virens IMI-392430 + A. tenuis), and T3 (T. pseudokoningii IMI-392431 + A. tenuis) treatment, while single treatment with A. tenuis significantly decreased these values.

• Mycobiology
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• v.51 no.3
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• pp.178-185
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• 2023

The cell wall integrity (CWI) signaling pathway plays important roles in the dissemination and infection of several plant pathogenic fungi. However, its roles in the pepper fruit anthracnose fungus Colletotrichum scovillei remain uninvestigated. In this study, the major components of the CWI signaling pathway-CsMCK1 (MAPKKK), CsMKK1 (MAPKK), and CsMPS1 (MAPK)-were functionally characterized in C. scovillei via homology-dependent gene replacement. The ΔCsmck1, DCsmkk1, and ΔCsmps1 mutants showed impairments in fungal growth, conidiation, and tolerance to CWI and salt stresses. Moreover, ΔCsmck1, ΔCsmkk1, and ΔCsmps1 failed to develop anthracnose disease on pepper fruits due to defects in appressorium formation and invasive hyphae growth. These results suggest that CsMCK1, CsMKK1, and CsMPS1 play important roles in mycelial growth, conidiation, appressorium formation, plant infection, and stress adaption of C. scovillei. These findings will contribute to a better understanding of the roles of the CWI signaling pathway in the development of pepper fruit anthracnose disease.

• Journal of Bio-Environment Control
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• v.13 no.3
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• pp.156-161
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• 2004

This study was conducted to investigate the effects of soil amendment with different characteristics on plant growth, fruit yield and quality of oriental melon for continuous cropping under protected cultivation. Humus layers in arable soil was disturbed because soil amendment from hillside to oriental melon field was continued to resolve problems for continuous cropping. Water potential and hardness of soil was decreased in sandy loam with lower clay contents compared with loam and silty clay. Leaf length and area, fresh and dry weight of plant at earlier growing stage were higher, but chlorophyll contents of leaves were dropped in sandy loam compared with silty clay soil. Fruit size and weight was higher in sandy loam, but soluble solid and color of fruit were increased in silty clay. Marketable and unmarketable yield and quantity of fermented fruit were the highest in sandy loam. Hardness and weight of fruit were decreased by longer storage period and soluble solids of fruit was peaked at 5 day after storage, but decreased by prolonged continued storage. Because of these results, soil characteristics of amendment to oriental melon field should be considered as an important factor for quality and yield of oriental melon.

• The Plant Pathology Journal
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• v.37 no.1
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• pp.24-35
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• 2021

Blue mold caused by Penicillium expansum is one of the most significant postharvest diseases of apples. Some microorganisms associated with the surface of ripening apples possess the ability to inhibit the growth of P. expansum. However, the existing literature about their colonization in the stages before ripening is not explored in depth. This study aims to characterize the antagonistic capacity of bacterial populations from five fruit development stages of 'Royal Gala' apples. The results have shown that the density of the bacterial populations decreases throughout the ripening stages of fruit (from 1.0 × 105 to 1.1 × 101 cfu/㎠). A total of 25 bacterial morphotypes (corresponding to five genera identified by 16S RNA) were differentiated in which Bacillus stood out as a predominant genus. In the in vitro antagonism tests, 10 Bacillus strains (40%) inhibited the mycelial growth of P. expansum from 30.1% to 60.1%, while in fruit bioassays, the same strains reduced the fruit rot ranging from 12% to 66%. Moreover, the bacterial strains with antagonistic activity increased in the ripening fruit stage. B. subtilis subsp. spiziennii M24 obtained the highest antagonistic activity (66.9% of rot reduction). The matrix-assisted laser desorption ionization-time of flight mass spectrometry analysis revealed that bacteria with antagonistic activity produce anti-fungal lipopeptides from iturin and fengycin families.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.4
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• pp.349-358
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• 2014

This study was conducted to determine the impact of elevated temperature in growing season on the growth and fruit quality of red pepper (Capsicum annuum L.) by cultivating pepper in the temperature gradient tunnels. Plant height, stem diameter, leaf number and total leaf area, fresh weight and dry weight increased at ambient $+2^{\circ}C$ temperature, whereas each leaf area decreased as temperature increased. The plants grown under ambient $+2^{\circ}C$ temperature showed the greatest number of flower and fruit. Fruit weight, fruit length and fruit diameter decreased as the temperature increasing gradually. Total fruit number, total fruit weight and total dry fruit weight was the highest at ambient $+2^{\circ}C$ temperature. Major free sugars of red pepper fruit were fructose and glucose. Free sugar content of red pepper according to the differences in harvesting times and in growth temperature showed a little differences. The yield of red pepper fruit at ambient $+2^{\circ}C$ temperature increased by 13% compared with the control. However, the yield of red pepper fruit at ambient $+4^{\circ}C$ temperature decreased by 20% as compared to control. Non-marketable fruits (diseased fruit, malformed fruit and small sized fruit) increased as the temperature rised.

• Journal of Bio-Environment Control
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• v.5 no.1
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• pp.50-56
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• 1996

This experiment was carried out to investigate the effects of new material, bioceramic powder in the nutrient solution(0.02%) and foliar spray(0.2%) on the growth and fruit quality of aeroponically grown tomato(Lycopersicon esculentum MILL. cv. Seokwang). Plant height was lowered, but root growth was increased both treatment of bioceramic powder into the nutrient solution and foliar spray. Cluster growth of tomato plants increased up to 14% over than that of control in the plot of foliar spray with bioceramic powder. Total plant dry weight also increased when bioceramic powder was treated both into the nutrient solution and foliar spray. T/R ratio was reduced by the treatment of bioceramic powder, but NAR and RGR showed the highest in the plot of foliar spray treated with 0.2% bioceramic powder than that of the other plots. Cumulative fruit fresh and dry weight were reduced when bioceramic powder was treated into nutrient solution but foliar spray produced more cumulative fruit fresh and dry weight than that of control. It was shown that treatment of bioceramic powder into the nutrient solution and foliar spray could be improved sugar degree without reducing fruit yields.