• Asian Journal of Turfgrass Science
• /
• v.8 no.3
• /
• pp.137-147
• /
• 1994

We have established a high-frequency plant regeneration system via organogenesis from mature seed of Korean lawngrass(Zoysia japonica Steud.). The effects of 2,4-dichiorophenoxy acetic acid (2,4-D), 6-furfuryl amino purine (kinetin), $\alpha$-naphthaiene acetic acid (NAA), N6-benzyl amino pu-rine (BAP), and casein hydrolysate (CR) on cailus induction and multiple shoot formation on ex-posure to light were evaluated. Callus produced on the Murashige and Skoog (MS) medium containing 2,4-D and kinetin had high organogenesis potency. A single addition of 1.0 mg $L-^1$ 2,4-D significantly induced callus. Also, 1.0 mg L-$^1$ 2,4-D, with the addition of 0.1 mg $L-^1$ kinetin highly enlianced callus induction. The trend of cailus induction was also found on mediurn containing 0.1 mg $L-^1$ BAP with 1.0 mg $L-^1$2,4-D, and 1 g $L-^1$ CR with the addition of 1.0 mg $L-^1$ 2,4-D. However, NAA was no effective on callus formation. The growth of root was significantly high in the presence of 0.1 mg $L-^1$ kinetin compared to other concentrations. Over 2 mg $L-^1$ kinetin highly lengthened roots. Fresh weight of plantlet was highest on medium containing 0.1 mg $L-^1$ 2,4-D. Also, on medium containing 0.1 mg $L-^1$ BAP, fresh weight of piantlet was highly enhanced. BAP was significantly effective on multiple shoot formation, particularly when 2.0 mg $L-^1$ was added with 0.1 mg $L-^1$ 2,4-D. Callus induction and multiple shoot formation were achieved on MS basal medium containing 1.0 g $L-^1$ CH.

• Korean Journal of Plant Resources
• /
• v.30 no.6
• /
• pp.612-617
• /
• 2017

The preservation of pear germplasm, like that of other clonal germplasms, is difficult because it requires conservation of whole plants or their tissues. Among the currently available methods for long-term conservation of clonal germplasm, cryopreservation of shoot tips is the most reliable and cost- and space-effective option. Alginate-coated axillary shoot tips from in vitro-grown pear were conserved successfully in liquid nitrogen (LN) following dehydration. Shoot recovery from cryopreserved shoot tips was improved greatly after 8 weeks of cold acclimation, but recovery decreased slightly after then. The highest regeneration rate was observed when in vitro shoot tips were preincubated in MS (Murashige and Skoog) medium with 0.3 M sucrose for 48 h, and when alginate-coated shoot tips were precultured in MS medium with increasing sucrose concentrations (0.5 M and 0.7 M) for 8 and 16 h, respectively. When the encapsulated beads were dehydrated for up to 7 h [25% water content (fresh weight basis)] under laminar flow, the highest regeneration rate was observed in "BaeYun No. 3" (55.7%) and "Whanggeum" (43.3%) after warming from LN. This technique is useful as a practical procedure to cryopreserve plant material that is sensitive to freezing of the surrounding cryoprotectant medium. Therefore, this technique appears to be promising for the cryopreservation of shoot tips from in vitro-grown plantlets of pear germplasm.

• Journal of Bio-Environment Control
• /
• v.25 no.2
• /
• pp.89-94
• /
• 2016

This study was conducted to determine the optimum nutrient solution, pH, irrigation interval, light intensity and planting density to growth of common ice plant (Mesembryanthemum crystallinum L.) in a closed-type plant production system. Three-band radiation type fluorescent lamps with a 12-h photoperiod were used. Nutrient film technique systems with three layers were used for the plant growth system. Environmental conditions, such as air temperature, relative humidity and $CO_2$ concentration were controlled by an ON/OFF operation. Treatments were comparison of the nutrient solution of Horticultural Experiment Station in Japan (NHES) and the nutrient solution of Jeju National University (NJNU), pH 6.0 and 7.0, irrigation interval 5 min and 10 min, light intensity 90 and $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, and within-row spacing 10 cm, 15 cm, 20 cm and 25 cm with between-row spacing 15 cm. Optimum macronutrients were composed N 7.65, P 0.65, K 4.0, Ca 1.6 and Mg $1.0mM{\cdot}L^{-1}$. There were no significant interactions between pH 6.0 and 7.0 about shoot fresh weight and shoot dry weight of common ice plant. Irrigation interval 5 min and 10 min was also the same result. Shoot fresh weight and shoot dry weight were highest at $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Shoot fresh weight and shoot dry weight were decreased according to increasing the planting density. From the above results, we concluded that optimum nutrient solution, optimum levels of pH, irrigation interval, light intensity and planting density were 6.0-7.0 and 10 min, $180{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and $15{\times}15cm$, respectively for growth of common ice plant in a closed-type plant production system.

• Current Research on Agriculture and Life Sciences
• /
• v.32 no.4
• /
• pp.175-177
• /
• 2014

This is the first report describing the seasonal conditions affecting shoot regeneration by the chrysanthemum cv. Baeksun. The shoot regeneration from petal explants was found to be more favorable from September to December, reaching the highest values in December. In addition, the quality of the shoots was also influenced according to the season of the explant collection, where healthy and uniform plants were derived from the explants collected in December. Choosing the proper season for explant collection affected the successive plant growth parameters (i.e., plant height and fresh weight). Thus, the current results strongly suggest that season plays an important role in plant tissue culturing, which is an essential tool for micropropagation and Agro-bacterium-mediated genetic transformation studies.

• Journal of Bio-Environment Control
• /
• v.25 no.2
• /
• pp.106-110
• /
• 2016

In this study, a planting density-growth-harvest (PGH) chart was developed to easily read the growth and harvest factors such as crop growth rate, relative growth rate, shoot fresh weight, shoot dry weight, harvesting time, marketable rate, and marketable yield of common ice plant (Mesembryanthemum crystallinum L.). The plants were grown in a nutrient film technique (NFT) system in a closed-type plant factory using fluorescent lamps with three-band radiation under a light intensity of $140{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ and a photoperiod of 12 h. Growth and yield were analyzed under four planting densities ($15{\times}10cm$, $15{\times}15cm$, $15{\times}20cm$, and $15{\times}25cm$). Shoot fresh and dry weights per plant increased at a higher planting density until reached an upper limit and yield per area was also same tendency. Crop growth rate, relative growth rate and lost time were described using quadratic equation. A linear relationship between shoot dry weight and fresh weights was observed. PGH chart was constructed based on the growth data and making equations. For instance, with within row spacing (= 20 cm) and fresh weight per plant at harvest (= 100 g), we can estimate all the growth and harvest factors of common ice plant. The planting density, crop growth rate, relative growth rate, lost time, shoot dry weight per plant, harvesting time, and yield were $33plants/m^2$, $20g{\cdot}m^{-2}{\cdot}d^{-1}$, $0.27g{\cdot}g^{-1}{\cdot}d^{-1}$, 22 days, 2.5 g/plant, 26 days after transplanting, and $3.2kg{\cdot}m^{-2}$, respectively. With this chart, we could easily obtain the growth factors such as planting density, crop growth rate, relative growth rate, lost time and the harvest factors such as shoot fresh and dry weights, harvesting time, marketable rate, and marketable yield with at least two parameters, for instance, planting distance and one of harvest factors of plant. PGH charts will be useful tools to estimate the growth and yield of crops and to practical design of a closed-type plant production system.

• Korean Journal of Medicinal Crop Science
• /
• v.8 no.4
• /
• pp.319-326
• /
• 2000

This experiment was carried out to determine the effects of $GA_3$ treatment (concentration: period) to rhizomes before planting on their growth, morphology and yield. $GA_3$ treatment increased the number of shoots, leaves or latent buds, plant height, and leaf length per plant compared with non-treatment. The best treatment concentrations were 50 ppm in stem per plant, 100 ppm in latent bud and rhizome fresh weight per plant and 250 ppm in shoot fresh weight per plant. The number of leaves and roots per plant, plant height and fresh weights of shoot and root were the greatest in one day imbibition of $GA_3$. Numbers of stems and latent buds per plant and rhizome fresh weight, however, were the highest in two day imbibition. In addition, the most rhizomes per land unit were produced in one day imbibition treatment of 100 ppm $GA_3$.

• Horticultural Science & Technology
• /
• v.28 no.5
• /
• pp.768-776
• /
• 2010

This study was conducted to predict number and fresh weight of leaves, and total leaf area of a single-stemmed rose 'Vital' based on the accumulated thermal units, and to develop a model of shoot development for the prediction of the time when the flowering shoot reaches a phenological stage in a plant factory system. The base temperature ($T_b$), optimum temperature ($T_{opt}$), and maximum temperature ($T_{max}$) were estimated by regressing the rate of shoot development against the temperature gradient. The rate of shoot development ($R$, $d^{-1}$) for the phase from cutting to bud break (CT-BB) was best described by a linear model $R_b$ ($d^{-1}$) = -0.0089 + $0.0016{\cdot}temp$. The rate of shoot development for the phase from bud break to harvest (BB-HV) was fitted to the parabolic model $R_h$ ($d^{-1}$) = $-0.0001{\cdot}temp^2$ + $0.0054{\cdot}temp$ - 0.0484. The $T_b$, $T_{opt}$, and $T_{max}$ values were 5.56, 27.0, and $42.7^{\circ}C$, respectively. The $T_b$ value was used in the thermal unit computations for the shoot development. Number of leaves, leaf area (LA), and leaf fresh weight showed sigmoidal curves regardless of the cut time. The shoot development and leaf area model was described as a sigmoidal function using thermal units. Leaf area was described as LA = 578.7 $[1+(thermal units/956.1)^{-8.54}]^{-1}$. Estimated and observed shoot length and leaf fresh weight showed a reasonably good fit with 1.060 ($R^2=0.976^{***}$) and 1.043 ($R^2=0.955^{***}$), respectively. The average thermal units required from cutting to transplant and from transplant to harvest stages were $426{\pm}42^{\circ}C{\cdot}d$ and $783{\pm}24^{\circ}C{\cdot}d$, respectively.

• Korean Journal of Plant Tissue Culture
• /
• v.28 no.6
• /
• pp.329-333
• /
• 2001

Callus and shoot formation from medicinal crop, Lycium chinense Mill. cv. 'Cheongyang', as influenced by various media, explant sources and plant growth regulators were investigated. The rate of shoots formation, number of shoots, and fresh weight of shoots were the best on MS medium followed by B$_{5}$, WPH, and SH. Callus induction was more effective in leaf than internode segments, and was the best on MS medium containing 0.5 mg/L NAA with 0.2 mg/L BA. Effects of plant growth regulators in shoot formation were more effective in BA than TDA combined with NAA. Shoot formation from callus induced in leaf and internode segments was the best on MS medium containing 0.01 mg/L NAA with 0.2 mg/L BA.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.5
• /
• pp.394-406
• /
• 2004

A split-plot designed experiment including four rice varieties and 10 nitrogen levels was conducted in 2003 at the Experimental Farm of Seoul National University, Suwon, Korea. Before heading, hyperspectral canopy reflectance (300-1100nm with 1.55nm step) and nine crop variables such as shoot fresh weight (SFW), leaf area index, leaf dry weight, shoot dry weight, leaf N concentration, shoot N concentration, leaf N density, shoot N density and N nutrition index were measured at 54 and 72 days after transplanting. Grain yield, total number of spikelets, number of filled spikelets and 1000-grain weight were measured at harvest. 14,635 narrow-band NDVIs as combinations of reflectances at wavelength ${\lambda}l\;and\;{\lambda}2$ were correlated to the nine crop variables. One NDVI with the highest correlation coefficient with a given crop variable was selected as the NDVI of the best fit for this crop variable. As expected, models to predict crop variables before heading using the NDVI of the best fit had higher $r^2$ (>10\%)$ than those using common broad- band NDVI red or NDVI green. The models with the narrow-band NDVI of the best fit overcame broad- band NDVI saturation at high LAI values as frequently reported. Models using NDVIs of the best fit at booting showed higher predictive capacity for yield and yield component than models using crop variables.

• Journal of Plant Biology
• /
• v.35 no.1
• /
• pp.17-23
• /
• 1992

Effects of polyamines and their biosynthetic inhibitors on adventitions shoot regeneration of Populus leaf segments were investigated. Polyamine inhibitors such as difluoromethyl arginine (DFMA), difluoromethyl ornithine (DFMO), and dicyclohexylamine (DCHA) decreased the fresh weight of cultured leaf segment and the rate of adventitious shoot regeneration. The inhibitory effects of DCHA were stronger than any other oplyamine inhibitors, and the rest were in the order of DFMO and DFMA. The inhibitory effects of these inhibitors were lessened or disappeared by the addition of polyamines, among which spermidine was the highest in its effect. Therefore, it is suggested that the spermidine may be related to the adventitious shoot regeneration of Populus leaf segments.gments.