• Food Science of Animal Resources
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• v.41 no.6
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• pp.1012-1021
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• 2021

This study evaluated the effect of freezing rate on the quality characteristics of pork loin to establish an objective standard for rapid freezing. To generate various freezing rates, three air flow rates (0, 1.5, and 3.0 m/s) were applied under three freezing temperatures (-20℃, -30℃, and -40℃). Based on the results, freezing rates ranged from 0.26-1.42 cm/h and were graded by three categories, i.e, slow (category I, >0.4 cm/h), intermediate (category II, 0.6-0.7 cm/h) and rapid freezing (category III, >0.96 cm/h). Both temperature and the air flow rate influenced the freezing rate, and the freezing rate affected the ice crystal size and shear force in pork loin. However, the air flow rate did not affect thawing loss, drip loss or the color of pork loins. In the comparison of freezing rates, pork belonging to category II did not show a clear difference in quality parameters from pork in category I. Furthermore, pork in category III showed fresh meat-like qualities, and the quality characteristics were clearly distinct from those of category I. Although the current standard for rapid freezing rate is 0.5 cm/h, this study suggested that 0.96 cm/h is the lowest freezing rate for achieving meat quality distinguishable from that achieved with conventional freezing, and further increasing the freezing rate did not provide advantages from an energy consumption perspective.

• International Journal of Highway Engineering
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• v.7 no.2 s.24
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• pp.87-95
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• 2005

To prevent the lowering of structural capability due to freezing and thawing in cold winter, numerous researches on frost heaving have been performed. As the result, the freezing index contour map of the Korea peninsula has been made for the design of the anti-freezing layer of pavements. However, the validity of the anti-freezing layer needs to be evaluated because systematic investigations on the variation of freezing depth with the thickness and material types of pavement layers and the configuration of the ground have been rarely performed. The freezing index of the Korea Highway Corporation test road site was calculated and the freezing depths of the concrete and asphalt pavements of the test road were investigated using the ambient and pavement temperature and water content. In addition, the investigated freezing depths were compared to the values estimated by existing freezing depth models. This is the preliminary study on the freezing-related data measured at the test road. The results with higher reliability will be produced by the long-term accumulation of the data and the analysis on it.

• Journal of the Korean Geosynthetics Society
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• v.11 no.3
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• pp.27-35
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• 2012

In this paper, the frost heaving and thawing characteristics of flexible pavement structure were evaluated in the large scale freezer which have a specification of temperature range $-20^{\circ}C{\sim}10^{\circ}C$ and $3.2m(L){\times}3.2m(B){\times}2.4m(H)$ in size. The insulated steel box with the size of $0.9m(L){\times}0.9m(B){\times}0.9m(H)$ was used to simulate actual pavement road structure. The variation of temperature, frost heave amount and frost heave pressure were measured through the instrument of TDS-602 data logger. LFWD (light falling weight deflectometer) was used to determine the change of deflection due to the frost heaving and thawing. Furthermore, the influence of aggregate layer to the freezing of the subgrade soil was studied to verify the function and effectiveness of the anti-freezing layer.

• Journal of the Korean Geotechnical Society
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• v.32 no.6
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• pp.17-26
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• 2016

Characteristic behaviors of geo-structure during freezing and thawing process have to be understood based on fundamental knowledge on phase change in porous soil and interaction between soil and structure. Inversion analysis using published one-dimensional soil freezing tests was conducted to suggest a mechanical model to consider an effect of the ice saturation on Young's modulus. Silty soil was more sensitive to temperature than weathered granite soil and sand, and weathered granite soil was more affected by initial water saturation in stiffness decrease than silty soil. Numerical simulations on chilled gas pipeline showed that shielding effect from surrounding frozen zone around the pipe decreases impact from external load onto the pipe. And a pipe installed in sand backfill showed more heaving due to relatively low stiffness of sand during freezing than that of surrounding in-situ weather granite soil. However, it had more stable stress condition due to effective stress redistribution from external load.

• Journal of Embryo Transfer
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• v.26 no.3
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• pp.187-193
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• 2011

This study was carried out to establish most suitable freezing condition, to evaluate the different glycerol concentration of freezing and thawing rates on motility, viability, membrane integrity and acrosome intecrity of frozen Korean Jeju Black Bull spermatozoa, Semen was collected from a Korean Jeju Black Bull using an artificial vagina and transported to the laboratory. The semen was extended gradually 1:5 then cooled slowly for 2 hrs to 4$^{\circ}C$. The semen was diluted 1:1 with cryoprotectant extenders (3%, 5% and 7% glycerol) and equilibrated for 2 hrs at cold chamber and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 min and above 8 cm for 10 min. And then the frozen straw was plunged into LN$_2$. The presented straws were examined the viability and motility after thawed at 37$^{\circ}C$ water bath. The viability and membrane integrity immediately post-thawing were significantly higher in samples frozen in 7% glycerol than 3% and 5% glycerol (p<0.05). After CTC staining to assess acrosome integrity, F pattern was significantly increased, but B pattern was significantly decreased in 7% glycerol (p<0.05). Freezing distance of 5 cm from liquid nitrogen and pre-cooling for 10 min yield better survival and membrane integrity, but not significant difference. However, AR pattern according to CTC staining was significantly decreased in 3 cm for 5 min.

• Korean Journal of Agricultural Science
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• v.40 no.3
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• pp.197-202
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• 2013

This study was aimed to find the storage potential of low temperature adapted shiitake mushroom under freezing temperature ($-3^{\circ}C$). Fresh shiitake mushroom was harvested at late Feb. and cooled to target temperature, $-3^{\circ}C$ in the cooling room for 24 hr. Cooled mushroom were then placed in plastic boxes, wrapped with plastic film ($30{\mu}m$), and stored at $-3^{\circ}C$ for 2 months. Weight loss of mushroom was ranged from 2.1 to 3.2%. Dry weight per unit fresh weight, however, was slightly increased because of moisture loss. Firmness of fruit body increased from $0.95kg/cm^{-2}$ (before storage) to $1.13kg/cm^{-2}$ (after 2 month storage). About half amount of starch was lost during 2 month storage. The amount of total and reducing sugars remained relatively constant. After storage, freshness of mushroom was recovered by thawing treatment. When recovered mushroom were packaged with styrofoam tray and PVC wrapping, and exposed to ambient and $10^{\circ}C$, respectively, brown spot on the gill of fruit body was found and slight decay symptom was also found at ambient temperature only but not at $10^{\circ}C$. Results indicated that low temperature adapted shiitake mushroom has a storage potential under freezing temperature ($-3^{\circ}C$). Freezing storage technology of fresh shiitake mushroom will contribute the increase of storability up to 2 months.

• Proceedings of the Korea Concrete Institute Conference
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• 1991.10a
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• pp.34-39
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• 1991

It is well-known that concretes under sea water environment show remarkably degrading phenomena physically by repeatable freeze-thawing action and chemically by penetration of soluted ions in sea water. In this study the influences of type of blending materials, their dosage and W/C ratio on freeze-thawing resistances of hardened cement concrete using foy ash, ground blast furnace slag, silica fume, EVA and SBR under sea-water environment were investigated.

• Korean Journal of Animal Reproduction
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• v.20 no.2
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• pp.207-214
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• 1996

To examine the developmental capacity of manipulated embryos after ultrarapid refreezing and thawing, mouse embryos were biopsied at 4-cell stage, frozen twice at 4-cell and morula stages, respectively, and then transferred to rec-ipients. Single blastomeres were biopsied from 4-cell embryos by a modified aspiration method. Biopsied 4-cell embryos were equilibrated into freezing medium at room temperature for 2.5 min, loaded into 40 $\mu$I of freezing medium in 0.25 ml plastic straw and then directly immersed into liqiud nitrogen. Freezing medium for 4-cell embryos consisted of 4.0 M ethylene glycol and O.25 M sucrose in dPBS supplemented with 6 mg/lm BSA. Morulae were frozen into freezing medium containing 5.0 M glycerol instead of ethylene glycol. Thawing was conducted by agitating each straw in 3TC water for 20 sec. The c content of each straw was expelled into 0.5 ml of dilution medium, which consisted of 0.25 M sucrose and 3 mg/ml BSA in dPBS. The thawed embryos were rehydrated in dilution medium for 10 min, washed 3 times with dPBS and then cultured in M16 medium at 37$^{\circ}C$, 5% CO$_2$ in air. Blastocysts that developed from frozen or refrozne biopsied embryos were transferred to recipients on Day 3 of pseudopregnancy, respectively. In vitro and in vivo developmental rates of the biopsied and intact 4~cell embryos after freezing and thawing were 78 (10l/130) and 25% (10/40), and 91 (114/125) and 30% (12/40), respectively. Although the rates of in vitro development of biopsied and intact embryos to blastocyst stage were significantly different after freezing and thawing (P

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.3
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• pp.1-7
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• 2018

As part of a research dedicated to the field evaluation of the durability of concrete subjected to freezing-thawing, this study analyzes the relationship between the surface roughness and the relative dynamic elastic modulus through image analysis. Four mix compositions with water-to-binder ratios (W/B) of 40%, 50%, 60% and 70% and without AE agent were considered to provoke early freezing. The basic physical properties of the mixes including the relative dynamic elastic modulus and the compressive strength were first evaluated experimentally according to W/B. Then, tests were performed to measure the surface roughness followed by photographs and SEM image analysis. The measured surface roughness tended to increase with larger number of freezing-thawing cycles regardless of W/B. The relative dynamic elastic modulus appeared to increase gradually with the number of cycles for the relatively denser mixes with W/B of 40% and 50%. Besides, the surface roughness increased only at rupture for the mixes with W/B of 60% and 70%. Moreover, the analysis of the photographs of the surface of the mixes with W/B of 40% and 50% revealed that the degradation progressed gradually from the surface with the freezing-thawing cycles. However, for the mixes with W/B of 60% and 70%, apparent change of the surface remained very insignificant until rupture at which damage like cracking could be observed. Consequently, the analysis of surface photograph or the measurement of the surface roughness presented some limitation in assessing the degree of freezing-thawing-induced degradation in case of relatively porous specimens. On the other hand, the photograph and surface roughness appeared to be sufficient for assessing such degradation for the mixes with W/B of 40% and 50%. Accordingly, the image of the surface and the surface roughness are potentially applicable on site for the assessment of freezing-thawing damages in relatively dense mixes.

• Journal of the Korea institute for structural maintenance and inspection
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• v.13 no.6 s.58
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• pp.126-134
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• 2009

Generally, reinforced concrete structures exposed to the outside temperature are affected by freezing and thawing process during winter and early spring. These freeze-thawing process can lead to the reduction in durability of concrete as cracking or surface spalling. This paper is to study the flexural behavior of RC beams exposed to freeze-thawing environments. To compare the difference in flexural behavior of RC Beams, limited tests were conducted under different types of Longitudinal steel ratio and freeze-thawing cycles. For this purpose, fourteen small-scale RC beams ($100mm{\times} 100mm {\times}600mm$) were strengthened in monotonic and cyclic loadings, subjected to up to 150, 300 cycles freeze-thawing from $-18{\sim}4^{\circ}C$. It is thought that experimental results will be used as basic data to evaluate flexural behavior of RC beams exposed to freeze-thawing.