Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Change of Sperm Viability and Acrosome Integrity of Post-thawed Korean Jeju Black Bull Spermatozoa according to Glycerol Concentration

제주 흑우 동결 정액 제조에 있어 Glycerol의 농도에 따른 생존율 및 정자 첨체 양상의 변화

  • Choi, Sun-Ho (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Ko, Min-Hee (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Kang, Tae-Young (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Park, Yong-Sang (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA) ;
  • Oh, Shin-Ae (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
  • 최선호 (국립축산과학원 난지축산시험장) ;
  • 고민희 (국립축산과학원 난지축산시험장) ;
  • 강태영 (국립축산과학원 난지축산시험장) ;
  • 조상래 (국립축산과학원 난지축산시험장) ;
  • 박용상 (국립축산과학원 난지축산시험장) ;
  • 오신애 (국립축산과학원 난지축산시험장)
  • Received : 2011.08.08
  • Accepted : 2011.08.30
  • Published : 2011.09.30
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Abstract

This study was carried out to establish most suitable freezing condition, to evaluate the different glycerol concentration of freezing and thawing rates on motility, viability, membrane integrity and acrosome intecrity of frozen Korean Jeju Black Bull spermatozoa, Semen was collected from a Korean Jeju Black Bull using an artificial vagina and transported to the laboratory. The semen was extended gradually 1:5 then cooled slowly for 2 hrs to 4$^{\circ}C$. The semen was diluted 1:1 with cryoprotectant extenders (3%, 5% and 7% glycerol) and equilibrated for 2 hrs at cold chamber and packed to 0.5 ml straws. The semen straws were located above 3 cm of liquid nitrogen for 5 minutes, above 5 cm for 10 min and above 8 cm for 10 min. And then the frozen straw was plunged into LN$_2$. The presented straws were examined the viability and motility after thawed at 37$^{\circ}C$ water bath. The viability and membrane integrity immediately post-thawing were significantly higher in samples frozen in 7% glycerol than 3% and 5% glycerol (p<0.05). After CTC staining to assess acrosome integrity, F pattern was significantly increased, but B pattern was significantly decreased in 7% glycerol (p<0.05). Freezing distance of 5 cm from liquid nitrogen and pre-cooling for 10 min yield better survival and membrane integrity, but not significant difference. However, AR pattern according to CTC staining was significantly decreased in 3 cm for 5 min.

Keywords

References

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