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Identification and Characterization of Hydrogen Peroxide-generating Lactobacillus fermentum CS12-1

  • Kang, Dae-Kyung;Oh, H.K.;Ham, J.-S.;Kim, J.G.;Yoon, C.H.;Ahn, Y.T.;Kim, H.U.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.1
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    • pp.90-95
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    • 2005
  • Lactic acid bacteria were isolated from silage, which produce high level of hydrogen peroxide in cell culture supernatant. The 16S rDNA sequences of the isolate matched perfectly with that of Lactobacillus fermentum (99.9%), examined by a 16S rDNA gene sequence analysis and similarity search using the GenBank database, thus named L. fermentum CS12-1. L. fermentum CS12-1 showed resistance to low pH and bile acid. The production of hydrogen peroxide by L. fermentum CS12-1 was confirmed by catalase treatment and high-performance liquid chromatography. L. fermentum CS12-1 accumulated hydrogen peroxide in culture broth as cells grew, and the highest concentration of hydrogen peroxide reached 3.5 mM at the late stationary growth phase. The cell-free supernatant of L. fermentum CS12-1 both before and after neutralization inhibited the growth of enterotoxigenic Escherichia coli K88 that causes diarrhea in piglets.

FLUID-BODY INTERACTION ANALYSIS OF FLOATING BODY IN THREE DIMENSIONS (3차원 부유체의 유체-물체 연성해석)

  • Go, G.S.;Ahn, H.T.
    • Journal of computational fluids engineering
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    • v.20 no.2
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    • pp.103-108
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    • 2015
  • Fluid-body interaction analysis of floating body with six degree-of-freedom motion is presented. In this study, three-dimensional incompressible Navier-Stokes equations are employed as a governing equation. The numerical method is based on a finite-volume approach on a cartesian grid together with a fractional-step method. To represent the body motion, the immersed boundary method for direct forcing is employed. In order to simulate the coupled six degree-of-freedom motion, Euler's equations based on rigid body dynamics are utilized. To represent the complex body shape, level-set based algorithm is utilized. In order to describe the free surface motion, the volume of fluid method utilizing the tangent of hyperbola for interface capturing scheme is employed. This study showed three different continuums(air, water and body) are simultaneously simulated by newly developed code. To demonstrate the applicability of the current approach, two different problems(dam-breaking with stationary obstacle and water entry) are simulated and all results are validated.

A Study on Street Fashion of Korean Teenager -Since the Latter Half of the `90s- (한국 청소년의 거리패션 분석 연구 -1990년대 후반을 중심으로-)

  • 김주영;김소영;양숙희
    • The Research Journal of the Costume Culture
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    • v.5 no.3
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    • pp.96-117
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    • 1997
  • The purpose of this study is focused on revealing teenager characteristics and mass culture in the end of the 20th century, and researching the general traits of teenager costume in the latter half of the \`90s and the typical style based on the analysis of the teenager culture. The result of this study are following as; The general traits of teenager costume are classified with sports-orientation, brand-orientation, and foreign street fashion-orientation. Typical styles are classified with sportive look, hiphop look, and funny look. Sportive look became the core of the street fashion developed with the street sports in the city, and presented the practical use with the fashionability mixing sportswear such as hightech snickers, sports character wear and items. Hiphop look, the genderless fashion, expresses teenager\`s free life style and the diversiied sensibility and deconstructs the border of gender, racism. Funny look accepts the burden of the millenium as a humor and presents katharsis by creating unexpected style. The contemporary costume of teenager deconstructs the fixed idea about mix and match, good taste and bad taste, gender, coordination suited with T.P.O and intends ‘open costume’ for 21st century.

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Studies on the Clinical Significance of Free Thyroxine Concentration in Serum by Radioimmunoassay (방사면역측정법(放射免疫測定法)에 의한 혈중(血中) 유리(遊離) Thyroxine 농도측정(濃度測定)의 임상적(臨床的) 의의(意義)에 대(對)한 검토(檢討))

  • Lee, Joon-Il
    • Journal of radiological science and technology
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    • v.11 no.1
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    • pp.25-31
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    • 1988
  • Studies on the clinical significance with Amerlex $FT_{4}$ RIA kit observing the determination of $FT_{4}$ were investigated using a tracer as $^{125}I-T_{4}$ derivative which is not almostly bound to thyroxine binding globulin, etc. The results are followed; 1. $FT_{4}$ value($1,55{\pm}0.38ng/100ml$) of normal group was not accorded that of hyperthyroidism with Amerlex $FT_{4}$ RIA kit, and was higher than that of hypothyroidism. 2. $FT_{4}$ value was lower level in chronic-kidney disfunction syndrome whereas, it was normal in a cancer patient, a woman in pregnancy and a patient in TBG disfunction. 3. The value of this method is a good corelationship at that of equilibrium dialysis method. (r=0.931) 4. $FT_{4}$ value by this kit was linear relationship to those of the other kit (Gamma Coat and Liquisol), and the normal value of each methods was also similar. As mentioned above, this method is more simple and rapid, compared to the other method. Therefore, it was thought that this method is a very useful clinically.

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The Effects of an Obesity-Management Program on Body Composition, Abdominal Circumference, and lipid Metabolism for Middle-Aged Obese Women (중년 비만여성에게 적용한 비만관리프로그램이 체성분, 복부둘레 및 지질대사에 미치는 효과)

  • Oh, Hyo-Sook;Sim, Mi-Jung;Oh, Hyun-Ei
    • Journal of Korean Biological Nursing Science
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    • v.12 no.2
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    • pp.89-96
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    • 2010
  • Purpose: This study was aimed to develop and evaluate an obesity-management program for middle-aged obese women. Methods: Two 12 week session of the obesity-management programs constituted of weekly education and exercise such as aquarobic, pilates & yoga, and power-walking during 60 min for 3 days a week from June to December, 2006. The subjects included 47 middle-aged obese women between 30 and 60 yr. The effectiveness of the program was evaluated according to the change of body weight, body component, abdominal circumference, and serum lipid concentration. Data were measured by Inbody, tape measure, and blood test. Data were analyzed by descriptive statistics, and a paired t-test with an SPSS/PC. Results: There were statistically significant decreases in body weight, body mass index, body fat mass, Hemoglobin, abdominal circumference, total cholesterol level, and low density lipoprotein after the program was completed. But there were not statistically significant difference in fat free mass, muscle mass and triglyceride. Conclusion: The results of this study showed that an obesity-management program have positive effects in body composition, abdominal circumference, and lipid metabolism for middle-aged obese women.

Influence of External Reinforcement on Strain Characteristics of Critical Current in BSCCO Superconducting Tapes

  • Shin, Hyung-Seop;Kazumune Katagiri
    • Progress in Superconductivity and Cryogenics
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    • v.5 no.3
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    • pp.15-19
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    • 2003
  • For the purpose of standardization of the critical current measurement, it is meaningful to describe how $I_{c}$ will behave as the stress/strain level changes. In this study, strain dependencies of the critical current $I_{c}$ in Ag-alloy sheathed multifilamentary Bi(2212) and Bi(2223) superconducting tapes were evaluated at 77K, 0T. The external reinforcement was accomplished by soldering AgMgNi alloy tapes onto single or both sides of the sample. With the external reinforcement to the Bi(2212) tape, the strength of the tapes increased but the critical current at the strain free state, $I_{c0}$ decreased in some cases. The strain for onset of the $I_{c}$ degradation, $\varepsilon$$_{\irr}$, increased with an increase of the reinforcing volume and then saturated to a certain value. The effect of external reinforcement on the degradation of $I_{c}$ due to the bending strain in the Bi(2223) tape was also examined. Contrary to the expectation, it showed a significant $I_{c}$ degradation even at a small strain of 0.4 %. The observations of damage morphologies gave a good explanation to the $I_{c}$ behavior.c/ behavior.r.

In vitro Antioxidant and Cytoprotective Activities of the Extract of Dangyuja (Citrus grandis Osbeck) Leaves

  • Kim, Yun-Jung;Cho, Moon-Jae;Kim Cho, So-Mi
    • Food Science and Biotechnology
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    • v.17 no.5
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    • pp.1086-1091
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    • 2008
  • The antioxidant activities of the extracts of dangyuja (Citrus grandis Osbeck) leaves were evaluated. The highest phenolic content was obtained from the ethyl acetate fraction (EF) (202.1$\pm$0.8 mg GAE/g dried extract) and it exhibited the highest 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. The cytoprotective effects of EF on oxidative damage induced by tert-butyl hydroperoxide (t-BHP) in a human hepatoma cell line, HepG2 cells, were investigated to understand the intracellular antioxidant mechanisms. Treatment of HepG2 cells with EF prior to oxidative stress was found to inhibit reactive oxygen species (ROS) generation, lipid peroxidation, and DNA damage in a dose-dependent manner. Gas chromatography-mass spectrometry (GC-MS) studies on EF resulted in tentative identification of 19 compounds representing 94.3% of the total content. Taken together, these results demonstrated that EF has excellent antioxidant activities and thus dangyuja leaves have great potential as a source for natural antioxidant which can be applied in food products.

A Low-Power Portable ECG Touch Sensor with Two Dry Metal Contact Electrodes

  • Yan, Long;Yoo, Hoi-Jun
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.10 no.4
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    • pp.300-308
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    • 2010
  • This paper describes the development of a low-power electrocardiogram (ECG) touch sensor intended for the use with two dry metal electrodes. An equivalent ECG extraction circuit model encountered in a ground-free two-electrode configuration is investigated for an optimal sensor read-out circuit design criteria. From the equivalent circuit model, (1) maximum sensor resolution is derived based on the electrode's background thermal noise, which originates from high electrode-skin contact impedance, together with the input referred noise of instrumentation amplifier (IA), (2) 60 Hz electrostatic coupling from mains and motion artifact are also considered to determine minimum requirement of common mode rejection ratio (CMRR) and input impedance of IA. A dedicated ECG read-out front end incorporating chopping scheme is introduced to provide an input referred circuit noise of 1.3 ${\mu}V_{rms}$ over 0.5 Hz ~ 200 Hz, CMRR of IA > 100 dB, sensor resolution of 7 bits, and dissipating only 36 ${\mu}W$. Together with 8 bits synchronous successive approximation register (SAR) ADC, the sensor IC chip is implemented in 0.18 ${\mu}m$ CMOS technology and integrated on a 5 cm $\times$ 8 cm PCB with two copper patterned electrodes. With the help of proposed touch sensor, ECG signal containing QRS complex and P, T waves are successfully extracted by simply touching the electrodes with two thumbs.

Mechanical and Magnetic Properties of YBCO Superconductor with Bi/CNT Composite and Resin/CNT Impregnation (Bi/CNT 화합물과 Resin/CNT를 보강한 YBCO 초전도체의 기계적, 자기적 특성 변화)

  • Oh, W.S.;Jang, G.E.;Han, Y.H.;Sung, T.H.
    • Progress in Superconductivity
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    • v.9 no.1
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    • pp.107-110
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    • 2007
  • Bi/CNT composite and resin/CNT were chosen to improve the mechanical properties of $YBa_2Cu_3O_7$(YBCO) superconductor. In order to elucidate the effects of Bi/CNT composite and resin/CNT in YBCO superconductors, melt texture superconductor were impregnated by mixed compound of Bi and CNT into the artificial holes parallel to the c-axis, which were drilled on the YBCO superconductor. Various amount of Bi/CNT and resin/CNT were impregnated to YBCO superconductor with different holes diameters. Typical artificial holes diameters were 0.5, 0.7, and 1.0 mm respectively. Result of three-point bending test measurement, the bending strength with resin/CNT impregnation was improved up to 59.64 MPa as compared with 50.79 MPa of resin/CNT free bulk. Resin/CNT impregnation has been found to be one of the effective ways in improving the mechanical properties of bulk superconductor.

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Numerical Study of Thermal Choking Process in a Model SCRamjet Combustor (모델 스크램제트 연소기 내의 열적 질식 과정 수치 연구)

  • Lee, B.R.;Moon, G.W.;Jeung, I.S.;Choi, J.Y.
    • 한국연소학회:학술대회논문집
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    • 2000.12a
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    • pp.83-91
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    • 2000
  • A numerical study was carried out to investigate the 'unstart' process of thermally-choked combustion in model scramjet engines. The combustion mechanism of supersonic combustor will be compared with the experimental results obtained from the T3 free-piston shock tunnel at ANU (Australian National University) and the high enthalpy supersonic wind tunnel at UT (University of Tokyo). For the numerical simulation of supersonic combustion. multi-species Navier-Stokes equations were considered. and detailed chemistry reaction mechanism of $H_2$-Air were adopted. The governing equations were solved by Roe's FDS method and LU-SGS method with MUSCL scheme. In this study. it is found that the thermal choking process could result from excessive heat release due to combustion. In detail, sufficient heat release could be generated at local region of very high temperature increased by reflection of shock waves or vortex sheets. Accordingly the flow of downstream of the combustor fell to subsonic field propagated upstream along the combustor. Sometimes the subsonic flow field propagated into isolator could generate precombustion shock waves in the isolator.

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