Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Identification and Characterization of Hydrogen Peroxide-generating Lactobacillus fermentum CS12-1

  • Kang, Dae-Kyung (Bio-Resources Institute, EASY BIO System) ;
  • Oh, H.K. (Bio-Resources Institute, EASY BIO System) ;
  • Ham, J.-S. (National Livestock Research Institute, Rural Development Administration) ;
  • Kim, J.G. (National Livestock Research Institute, Rural Development Administration) ;
  • Yoon, C.H. (School of Agricultural Biotechnology, Seoul National University) ;
  • Ahn, Y.T. (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, H.U. (School of Agricultural Biotechnology, Seoul National University)
  • Received : 2004.05.19
  • Accepted : 2004.09.16
  • Published : 2005.01.01
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Abstract

Lactic acid bacteria were isolated from silage, which produce high level of hydrogen peroxide in cell culture supernatant. The 16S rDNA sequences of the isolate matched perfectly with that of Lactobacillus fermentum (99.9%), examined by a 16S rDNA gene sequence analysis and similarity search using the GenBank database, thus named L. fermentum CS12-1. L. fermentum CS12-1 showed resistance to low pH and bile acid. The production of hydrogen peroxide by L. fermentum CS12-1 was confirmed by catalase treatment and high-performance liquid chromatography. L. fermentum CS12-1 accumulated hydrogen peroxide in culture broth as cells grew, and the highest concentration of hydrogen peroxide reached 3.5 mM at the late stationary growth phase. The cell-free supernatant of L. fermentum CS12-1 both before and after neutralization inhibited the growth of enterotoxigenic Escherichia coli K88 that causes diarrhea in piglets.

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References

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