• Proceedings of the Korean Vacuum Society Conference
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• 2014.02a
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• pp.126-126
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• 2014

Label-free biomolecular assay based localized surface plasmon resonance (LSPR) of noble metal nanoparticles enables simple and rapid detection with the use of simple equipment. Nanosized metal nanoparticles exhibit a strong absorption band when the incident light frequency is resonant with the collective oscillation of the electrons, which is known as the LSPR. Here we demonstrate localized surface plasmon resonance (LSPR) substrates such as plasmonic Au nanodisks fabricated by a nanoimprinting process and gold nanorod-immobilized surfaces and their applications to highly sensitive and/or label-free biosensing. To increase detection sensitivity various bioreceptors weree designed. A single chain variable fragment (scFv) was used as a receptor to bind C-reactive protein (CRP). The results of this effort showed that CRP in human serum could be quantitatively detected lower than 1 ng/ml. Aptamers, which were immobilized on gold nanorods, were used to detect mycotoxins. The specific binding of ochratoxin A (OTA) to the aptamer was monitored by the longitudinal wavelength shift of LSPR peak in the UV-Vis spectra resulting from the changes of local refractive index near the GNR surface induced by accumulation of OTA and G-quadruplex structure formation of the aptamer. According to our results, OTA could be quantitatively detected lower than 1 nM level. Additionally, aptamer-functionalized GNR substrate was quite robust and can be regenerated many times by rinsing at 70 OC to remove bound target. During seven times of washing steps, the developed OTA sensing system could be reusable. Moreover, the proposed biosensor exhibited selectivity over other mycotoxins with an excellent recovery for detection in grinded corn samples, suggesting that the proposed LSPR based aptasensor plays an important role in label-free detection of mycotoxins.

• Journal of the Society of Naval Architects of Korea
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• v.29 no.4
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• pp.114-131
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• 1992

In the present work, a two-dimensional boundary-value problem for a large amplitude motion is treated as an initial-value problem by satisfying the exact body-boundary and nonlinear free-surface boundary conditions. The present nonlinear numerical scheme is similar to that described by Vinje and Brevig(1981) who utilized the Cauchy's theorem and assumed the periodicity in the horizontal coordinate. In the present thesis, however, the periodicity in the horizontal coordinate is not assumed. Thus the present method can treat more realistic problems, which allow radiating waves to infinities. In the present method of solution, the original infinite fluid domain, is divided into two subdomains ; ie the inner and outer subdomains which are a local nonlinear subdomain and the truncated infinite linear subdomain, respectively. By imposing an appropriate matching condition, the computation is carried out only in the inner domain which includes the body. Here we adopt the nonlinear scheme of Vinje & Brevig only in the inner domain and respresent the solution in the truncated infinite subdomains by distributing the time-dependent Green function on the matching boundaries. The matching condition is that the velocity potential and stream function are required to be continuous across the matching boundary. In the computations we used, if necessary, a regriding algorithm on the free surface which could give converged stable solutions successfully even for the breaking waves. In harmonic oscillation problem, each harmonic component and time-mean force are obtained by the Fourier transform of the computed forces in the time domain. The numerical calculations are made for the following problems. $\cdot$ Forced harmonic large-amplitude oscillation(${\omega}{\neq}0,\;U=0$) $\cdot$ Translation with a uniform speed(${\omega}=0,\;U{\neq}0$) The computed results are compared with available experimental data and other analytical results.

• The Plant Pathology Journal
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• v.33 no.5
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• pp.441-449
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• 2017

Citrus canker is an important bacterial disease of citrus in several regions of the world. Strains of Xanthomonas citri type-A (Xc-A) group are the primary pathogen where citrus canker occurs. After Xc-A entered the Northeast of Argentina in 1974, the disease spread rapidly from 1977 to 1980 and then slowed down and remained moving at slow pace until 1990 when it became endemic. Citrus canker was detected in Northwest Argentina in 2002. This paper presents the main steps in the fight of the disease and the management strategies that have been used to control citrus canker at this time. We think the process might be usefull to other countries with the same situation. Results from more than 40 years of research in Northeast (NE) Argentina indicate that we are at the limit of favorable environment for the disease. The severity of citrus canker is greatly affected by the environment and El $Ni{\tilde{n}}o$ Southern Oscillation (ENSO) phenomenon which causes cyclic fluctuations on the disease intensity in the NE region. Weather-based logistic regression models adjusted to quantify disease levels in field conditions showed that the environmental effect was strongly modulated by the distance from a windbreak. Production of healthy fruits in citrus canker endemic areas is possible knowing the dynamics of the disease. A voluntary Integrated Plan to Reduce the Risk of Canker has been in place since 1994 and it allows growers to export unsymptomatic, uninfested fresh fruit to countries which are free of the disease and require healthy, pathogen free fruits. The experience from Argentina can be replicated in other countries after appropriate trials.

• Ceramist
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• v.22 no.3
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• pp.281-300
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• 2019

Surface enhanced Raman scattering (SERS) was first discovered in 1974 by an unexpected Raman signal increase from Pyridine adsorbed on rough Ag electrode surfaces by the M. Fleishmann group. M. Moskovits group suggested that this phenomenon could be caused by surface plasmon resonance (SPR), which is a collective oscillation of free electrons at the surface of metal nanostructures by an external light source. After about 40 years, the SERS study has attracted great attention as a biomolecule analysis technology, and more than 2500 new papers and 500 review papers related to SERS topic have been published each year in recently. The advantages of biomaterials analysis using SERS are as follows; ① Molecular level analysis is possible based on unique fingerprint information of biomolecule, ② There is no photo-bleaching effect of the Raman reporters, allowing long-term monitoring of biomaterials compared to fluorescence microscopy, ③ SERS peak bandwidth is approximately 10 to 100 times narrower than fluorescence emission from organic phosphor or quantum dot, resulting in higher analysis accuracy, ④ Single excitation wavelength allows analysis of various biomaterials, ⑤ By utilizing near-infrared (NIR) SERS-activated nanostructures and NIR excitation lasers, auto-fluorescence noise in the visible wavelength range can be avoided from in vivo experiment and light damage in living cells can be minimized compared to visible lasers, ⑥ The weak Raman signal of the water molecule makes it easy to analyze biomaterials in aqueous solutions. For this reason, SERS is attracting attention as a next-generation non-invasive medical diagnostic device as well as substance analysis. In this review, the principles of SERS and various biomaterial analysis principles using SERS analysis will be introduced through recent research papers.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.22 no.5
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• pp.522-527
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• 2011

In this work, a 54 GHz divide-by-3 injection-locked frequency divider(ILFD) based on ring oscillator has been developed in a 0.13-${\mu}M$ Si RFCMOS technology for phase-locked loop(PLL) application. The free-running frequency is 18.92~19.31 GHz with tuning range of 0~1.8 V, consuming 70 mW with a 1.8 V supply voltage. At 0 dBm input power, the locking range is 1.02 GHz(54.82~55.84 GHz) and, with varactor tuning of 0~1.8 V, the total operating range is 2.4 GHz(54.82~57.17 GHz). The fabricated circuit size is 0.42 mm${\times}$0.6 mm including probing pads and 0.099 mm${\times}$0.056 mm for core area.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.196-196
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• 2012

Graphene, two-dimensional one-atom-thick planar sheet of carbon atoms densely packed in a honeycomb crystal lattice, has grabbled appreciable attention due to its extraordinary mechanical, thermal, electrical, and optical properties. Based on the graphene's high carrier mobility, high frequency graphene field effect transistors have been developed. Graphene is useful for photonic components as well as for the applications in electronic devices. Graphene's unique optical properties allowed us to develop ultra wide-bandwidth optical modulator, photo-detector, and broadband polarizer. Graphene can support SPP-like surface wave because it is considered as a two-dimensional metal-like systems. The SPPs are associated with the coupling between collective oscillation of free electrons in the metal and electromagnetic waves. The charged free carriers in the graphene contribute to support the surface waves at the graphene-dielectric interface by coupling to the electromagnetic wave. In addition, graphene can control the surface waves because its charge carrier density is tunable by means of a chemical doping method, varying the Fermi level by applying gate bias voltage, and/or applying magnetic field. As an extended application of graphene in photonics, we investigated the characteristics of the graphene-based plasmonic waveguide for optical signal transmission. The graphene strips embedded in a dielectric are served as a high-frequency optical signal guiding medium. The TM polarization wave is transmitted 6 mm-long graphene waveguide with the averaged extinction ratio of 19 dB at the telecom wavelength of $1.31{\mu}m$. 2.5 Gbps data transmission was successfully accomplished with the graphene waveguide. Based on these experimental results, we concluded that the graphene-based plasmonic waveguide can be exploited further for development of next-generation integrated photonic circuits on a chip.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.22 no.1
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• pp.47-57
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• 2010

Subgrid scale stabilization method is applied to Woo and Liu(2004)'s extended Boussinesq FEM numerical model to eliminate the 2dx wiggles. In order to optimize the computational efficiency, Hessian operator is introduced and the matrix of velocity vector is combined to one matrix for solving matrix equations. The mass lumping technique is also applied to the matrix equations of auxiliary variables. The newly developed code is applied to simulate Vincent and Briggs(1989)' wave transformation experiments and the results show that the numerical solution is almost wiggle-free and it matches very well with experimental data. Due to improvement of computational efficiency and wiggle reduction, it is plausible to apply this model to a realistic problem such as harbor oscillation problems.

• Journal of computational fluids engineering
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• v.13 no.4
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• pp.126-134
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• 2008

We developed a three dimensional Navier-Stokes code based on the level set method to simulate two phase flows with high density ratio. The Navier-Stokes equations with consideration of the surface tension effects are solved by using SIMPLE algorithm on a non-staggered grid. The present code is validated by simulating two test problems. First one is to simulate a rising bubble inside a cube. The thickness of the interface of the bubble is shown to affect the pressure distribution around the interface. As the thickness decreases, the pressure field around the interface becomes more oscillatory. As the bubble rises, a ring vortex is shown to form around the interface and the bubble eventually develops into an ellipsoidal shape. Merge of two bubbles inside a container is secondly tested to show the robustness of the present code for two phase flow simulation. Numerical results show stable and reliable behavior during the process of merging of two bubbles. The velocity and pressure fields around the interface of bubbles are shown oscillation free during the merging of two bubbles.

• Wind and Structures
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• v.14 no.5
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• pp.413-434
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• 2011

Without output covariance estimation, one reference-based Stochastic Subspace Technique (SST) for extracting modal parameters and flutter derivatives of bridge deck is developed and programmed. Compared with the covariance-driven SST and the oscillation signals incurred by oncoming or signature turbulence that adopted by previous investigators, the newly-presented identification scheme is less time-consuming in computation and a more desired accuracy should be contributed to high-quality free oscillated signals excited by specific initial displacement. The reliability and identification precision of this technique are confirmed by a numerical example. For the 3-DOF sectional models of Sutong Bridge deck (streamlined) and Suramadu Bridge deck (bluff) in wind tunnel tests, with different wind velocities, the lateral bending, vertical bending, torsional frequencies and damping ratios as well as 18 flutter derivatives are extracted by using SST. The flutter derivatives of two kinds of typical decks are compared with the pseudo-steady theoretical values, and the performance of $H_1{^*}$, $H_3{^*}$, $A_1{^*}$, $A_3{^*}$ is very stable and well-matched with each other, respectively. The lateral direct flutter derivatives $P_5{^*}$, $P_6{^*}$ are comparatively more accurate than other relevant lateral components. Experimental procedure seems to be more critical than identification technique for refining the estimation precision.

• Journal of Sensor Science and Technology
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• v.26 no.1
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• pp.66-72
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• 2017

The three dimensional ultrasonic anemometer was constructed to reduce the disadvantages of the two-dimensional anemometer and to be free from the use environment. Three pairs of transmitting and receiving ultrasonic sensors were designed to face each other at an angle of $45^{\circ}$ to the upper and lower surfaces at intervals of $120^{\circ}$. 200 kHz ultrasonic sensor Oscillation, transmission and reception, level detection, power supply circuit were designed and U, V, W wind speed vector components were obtained by measuring the time of first received ultrasonic pulse by transmitting pulse ultrasound. It is implemented as firmware in ARM Coretex-M3 processor so that horizontal and vertical wind direction and wind speed can be converted into digital signal by vector calculation. In this study, The three-dimensional ultrasonic anemometer can complement the disadvantages of the two-dimensional anemometer (mechanical and ultrasonic), and it is expected to gradually replace the two-dimensional anemometer due to its high utilization rate by collecting additional information such as vertical wind.