• The Journal of the Acoustical Society of Korea
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• v.14 no.2E
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• pp.57-64
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• 1995

In this study, we examine the applicability of the classifier based on an artifical neural network (ANN) for the low-frequency acoustic signal in shallow water environment. The estimations of the Doppler shift and frequency spreading effect at 220 Hz reveal the frequency variation of less than 2 Hz with time This small variation enables the ANN-based classifier to identify signals using only tonal frequency information. The ANN consists of 4 layers, and has 60 input processing elements (PEs) and 4 output PEs, respectively. When measured tonal signals in the frequency 200-250 Hz are applied to the ANN-based classifier, the classifier can identify more than 67% of the signals for instantaneous frame and more than 91% for averaged one over 5 frames.

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1653-1663
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• 2010

The first gene (${\alpha}$-gal1) encoding an extracellular ${\alpha}$-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The ${\alpha}$-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a 24 amino acid secretory signal sequence. The translated protein had highest identity with other fungal ${\alpha}$-galactosidases belonging to glycosyl hydrolase family 27. The ${\alpha}$-gal1 gene was overexpressed as a secretory protein with an N-terminal histidine tag in the methylotrophic yeast Pichia pastoris. Recombinant ${\alpha}$-Gal1 was secreted into the culture medium as a monomeric glycoprotein with a maximal yield of 10.75 mg/l and purified to homogeneity using Hisbinding nickel-agarose affinity chromatography. The purified enzyme was maximally active at $70^{\circ}C$, pH 4.5, and lost no activity over 10 days at $50^{\circ}C$. ${\alpha}$-Gal1 followed Michaelis-Menten kinetics ($V_{max}\;of\;240.3{\mu}M/min/mg,\;K_m\;of\;0.294 mM$) and was inhibited competitively by galactose ($K_m{^{obs}}$ of 0.57 mM, $K_i$ of 2.77 mM). The recombinant T. emersonii ${\alpha}$-galactosidase displayed broad substrate preference, being active on both oligo- and polymeric substrates, yet had strict specificity for the ${\alpha}$-galactosidic linkage. Owing to its substrate preference and noteworthy stability, ${\alpha}$-Gal1 is of particular interest for possible biotechnological applications involving the processing of plant materials.

• Journal of the Korean Institute of Intelligent Systems
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• v.24 no.5
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• pp.542-547
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• 2014

This paper describes the medical and IT convergence system using a smart phone and a heuristic method for the measurement of the dripping speed of the liquid in a drip chamber, which can estimate the remaining time using pattern recognition and difference image from video frame information based on Android technology. The video frames were first made using a smartphone camera and we calculated the difference image between the n image and the (n-1) image and then changed into binary images using the threshold value. At this point, it is very important to find an optimal threshold value using heuristic method to recognize the dripping of the liquids. In addition, the user can adjust the dripping speed according to the doctor's prescription, exactly like watching the progress bar of a mobile application. The experiment results show that our method using video processing technique accurately measures the dripping speed for a wide range of speeds that are sufficient for ordinary practice.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.46 no.2
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• pp.57-64
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• 2009

This paper presents the hardware implementation of the pyramidal KLT(Kanade-Lucas-Tomasi) feature tracker. Because of its high computational complexity, it is not easy to implement a real-time KLT feature tracker using general-purpose processors. A hardware implementation of the pyramidal KLT feature tracker using FPGA(Field Programmable Gate Array) is described in this paper with emphasis on 1) adaptive adjustment of threshold in feature extraction under diverse lighting conditions, and 2) modification of the tracking algorithm to accomodate parallel processing and to overcome memory constraints such as capacity and bandwidth limitation. The effectiveness of the implementation was evaluated over ones produced by its software implementation. The throughput of the FPGA-based tracker was 30 frames/sec for video images with size of $720{\times}480$.

• International Journal of Oral Biology
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• v.36 no.2
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• pp.43-50
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• 2011

Voltage dependent calcium channel (VDCC), one of the most important regulator of $Ca^{2+}$ concentration in neuron, play an essential role in the central processing of nociceptive information. The present study investigated the antinociceptive effects of L, T or N type VDCC blockers on the formalin-induced orofacial inflammatory pain. Experiments were carried out on adult male Sprague-Dawley rats weighing 220-280 g. Anesthetized rats were individually fixed on a stereotaxic frame and a polyethylene (PE) tube was implanted for intracisternal injection. After 72 hours, 5% formalin ($50 \;{\mu}L$) was applied subcutaneously to the vibrissa pad and nociceptive scratching behavior was recorded for nine successive 5 min intervals. VDCC blockers were administered intracisternally 20 minutes prior to subcutaneous injection of formalin into the orofacial area. The intracisternal administration of 350 or $700{\mu}g$ of verapamil, a blocker of L type VDCC, significantly decreased the number of scratches and duration in the behavioral responses produced by formalin injection. Intracisternal administration of 75 or $150 \;{\mu}g$ of mibefradil, a T type VDCC blocker, or 11 or $22\; {\mu}g$ of cilnidipine, a N type VDCC blocker, also produced significant suppression of the number of scratches and duration of scratching in the first and second phase. Neither intracisternal administration of all VDCC blockers nor vehicle did not affect in motor dysfunction. The present results suggest that central VDCCs play an important role in orofacial nociceptive transmission and a targeted inhibition of the VDCCs is a potentially important treatment approach for inflammatory pain originating in the orofacial area.

• Journal of Periodontal and Implant Science
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• v.23 no.3
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• pp.595-604
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• 1993

Since $Gr{\"{o}}nadhl$ et al introduced a digital subtraction radiography into periodontal dignosis in 1983, many reports using this system has aimed to assess the peroiodontal disease activity and the alveolar bone changes after periodontal treatment. The present study was performed to evaluate the usefulness of digital subtraction radiography for asessing an alveolar bone changes in 3 months after periodontal flap surgery. Serial intraral raiographs were taken from 5 normal subjects and 6 periodontally diseased patients using customized bite blocks attached to film holder in fxation device and digitized by image processing system(consisting of IBM 386, digital frame grabber, CCD camera, Image-pro II software). And the reference parameters were measured by pixel unit and compared with respective radiographs. The serial radiographs showed a little and statistically insignificant difference in reference paramenters. The conventional intraoral radiographs, the subtraction images and the clor enhanced subtraction images were reviewed by 4 examiners and the examiner's agreement rates were compared. The subtraction images and its color enhanced images showed higher examiner's agreement rate than the conventional radiographs. And the propotions of sites diagnosed with bone loss or gain after periodontal surgery were highter in the subtraction images and its color enhanced images than in the conventional radiographs. Especially, in color enhanced images, the unber of bone agin sites tended to increase according to post-surgery periods. These results indicate that projection geometry could be standardized with the divice used in this study, and the subtraction radiography may be useful to assess an alveolar bone changes after periodntal flap surgery.

• The KIPS Transactions:PartD
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• v.9D no.1
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• pp.111-126
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• 2002

As the technical aspect of the web system development is, unlike the existing main frame or client/server environment, greatly influenced by the dynamic and variable factors and carried out in a comparatively short period, practical and efficient process to which the developer may adapt with ease is very much required for the web system development. However, while the technological substitute, language and architecture composition have been much discussed, in connection with web system development growing rapidly, standardized development process and its deliverables have been rarely discussed. This thesis analyzes problems of the existing web system development processes and as the substitutes for them, proposes web system development process based on the domain features derived from the concepts of iteration and increment. For the classification of domains, five domains in total are set by interlocking web system factors and web site types and the workflows for two domains applied to projects are incorporated focused on work content and deliverables. The proposed process applies to the development of intranet system and is compared with the existing web development processes by the measure classified into components and elements.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.37A no.9
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• pp.786-791
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• 2012

This paper presents a depth boundary sharpening method for improved view synthesis in 3D video. In depth coding, distortion occurs around object boundaries, degrading the quality of synthesized images. In order to encounter this problem, the proposed method estimates an edge map for each frame to filter only the boundary regions. In particular, a window-based filter is employed to choose the most reliable pixel as the replacement considering three factors: frequency, similarity and closeness. The proposed method was implemented as post-processing of the deblocking filter in JMVC 8.3.Compared to the conventional methods, the proposed method generated 0.49 dB PSNR increase and 16.58% bitrate decrease on average. The improved portions were subjectively confirmed as well.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.36 no.2C
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• pp.86-92
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• 2011

This paper details the downlink performance analysis of an multiple antennas system that combines adaptive beamforming and spatial multiplexing (SM) Multiple Input Multiple Output (MIMO). The combination of MIMO signal processing with adaptive beamforming is applied to WiBro, the South Korean Orthogonal Frequency Division Multiple Access (OFDMA) system that follows the IEEE 802.16e standard. Performance analysis is based on the results of experiments and simulations obtained from a fixed-point simulation testbed. Simulations demonstrate that the MIMO Beamforming OFDMA system improves the required signal to noise ratio (SNR) over the conventional MIMO OFDMA system by 3 dB (QPSK) / 2.5 dB (16-QAM) for the frame error rate (FER) of 1% in the WiBro signal environments. From the implementation of the fixed-point simulation testbed and its experimental results, we verify the feasibility of the MIMO Beamforming technology for realizing a practical WiBro base station.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.34 no.7A
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• pp.540-547
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• 2009

In this paper, we work for the efficient use of the initial ranging contention slot in OFDMA based wireless mobile networks. Specially, the collision reduction method using an automatic adaptive optimal algorithm is studied for initial ranging contention slot used at initial connection of the mobile terminals. As a result, we propose the algorithm that achieves the collision minimization and the auto-dynamic slot arrangement of the initial ranging slots. To evaluate the proposed algorithm, we compare the simulation results of IEEE802.16e fixed initial ranging slot allocation method versus the proposed algorithm. The simulator is developed based on the IEEE802.16e standard MAC frame structure and processing procedures. As the simulation results, we can expect the proposed algorithm can be applied for the unmanned coastal base station because the proposed algorithm has the effect of minimizing administration cost for the base station.