International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
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Intracisternal Administration of Voltage Dependent Calcium Channel Blockers Attenuates Orofacial Inflammatory Nociceptive Behavior in Rats

  • Won, Kyoung-A. (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Park, Sang-H. (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Kim, Bo-K. (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Baek, Kyoung-S. (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Yoon, Dong-H. (Department of Oral Physiology, School of Dentistry, Kyungpook National University) ;
  • Ahn, Dong-K. (Department of Oral Physiology, School of Dentistry, Kyungpook National University)
  • Received : 2011.02.14
  • Accepted : 2011.04.15
  • Published : 2011.06.30
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Abstract

Voltage dependent calcium channel (VDCC), one of the most important regulator of $Ca^{2+}$ concentration in neuron, play an essential role in the central processing of nociceptive information. The present study investigated the antinociceptive effects of L, T or N type VDCC blockers on the formalin-induced orofacial inflammatory pain. Experiments were carried out on adult male Sprague-Dawley rats weighing 220-280 g. Anesthetized rats were individually fixed on a stereotaxic frame and a polyethylene (PE) tube was implanted for intracisternal injection. After 72 hours, 5% formalin ($50 \;{\mu}L$) was applied subcutaneously to the vibrissa pad and nociceptive scratching behavior was recorded for nine successive 5 min intervals. VDCC blockers were administered intracisternally 20 minutes prior to subcutaneous injection of formalin into the orofacial area. The intracisternal administration of 350 or $700{\mu}g$ of verapamil, a blocker of L type VDCC, significantly decreased the number of scratches and duration in the behavioral responses produced by formalin injection. Intracisternal administration of 75 or $150 \;{\mu}g$ of mibefradil, a T type VDCC blocker, or 11 or $22\; {\mu}g$ of cilnidipine, a N type VDCC blocker, also produced significant suppression of the number of scratches and duration of scratching in the first and second phase. Neither intracisternal administration of all VDCC blockers nor vehicle did not affect in motor dysfunction. The present results suggest that central VDCCs play an important role in orofacial nociceptive transmission and a targeted inhibition of the VDCCs is a potentially important treatment approach for inflammatory pain originating in the orofacial area.

Keywords

References

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