• Title/Summary/Keyword: forms modules

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Some characters of bacterial cellulases in goats' rumen elucidated by metagenomic DNA analysis and the role of fibronectin 3 module for endoglucanase function

  • Nguyen, Khanh Hoang Viet;Dao, Trong Khoa;Nguyen, Hong Duong;Nguyen, Khanh Hai;Nguyen, Thi Quy;Nguyen, Thuy Tien;Nguyen, Thi Mai Phuong;Truong, Nam Hai;Do, Thi Huyen
    • Animal Bioscience
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    • v.34 no.5
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    • pp.867-879
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    • 2021
  • Objective: Fibronectin 3 (FN3) and immunoglobulin like modules (Ig) are usually collocated beside modular cellulase catalytic domains. However, very few researches have investigated the role of these modules. In a previous study, we have sequenced and analyzed bacterial metagenomic DNA in Vietnamese goats' rumen and found that cellulase-producing bacteria and cellulase families were dominant. In this study, the properties of modular cellulases and the role of a FN3 in unique endoglucanase belonging to glycosyl hydorlase (GH) family 5 were determined. Methods: Based on Pfam analysis, the cellulases sequences containing FN3, Ig modules were extracted from 297 complete open reading frames (ORFs). The alkaline, thermostability, tertiary structure of deduced enzymes were predicted by AcalPred, TBI software, Phyre2 and Swiss models. Then, whole and truncated forms of a selected gene were expressed in Escherichia coli and purified by His-tag affinity column for assessment of FN3 ability to enhance enzyme activity, solubility and conformation. Results: From 297 complete ORFs coding for cellulases, 148 sequences containing FN3, Ig were identified. Mostly FN3 appeared in 90.9% beta-glucosidases belonging to glycosyl hydrolase family 3 (GH3) and situated downstream of catalytic domains. The Ig was found upstream of 100% endoglucanase GH9. Rarely FN3 was seen to be situated downstream of X domain and upstream of catalytic domain endoglucanase GH5. Whole enzyme (called XFN3GH5 based on modular structure) and truncate forms FN3, XFN3, FN3GH5, GH5 were cloned in pET22b (+) and pET22SUMO to be expressed in single and fusion forms with a small ubiquitin-related modifier partner (S). The FN3, SFN3 increased GH5 solubility in FN3GH5, SFN3GH5. The SFN3 partly served for GH5 conformation in SFN3GH5, increased modules interaction and enzyme-soluble substrate affinity to enhance SXFN3GH5, SFN3GH5 activities in mixtures. Both SFN3 and SXFN3 did not anchor enzyme on filter paper but exfoliate and separate cellulose chains on filter paper for enzyme hydrolysis. Conclusion: Based on these findings, the presence of FN3 module in certain cellulases was confirmed and it assisted for enzyme conformation and activity in both soluble and insoluble substrate.

A Study on Progressive Die Design by the using of Finite Element Method (유한요소법을 이용한 프로그레시브 금형 설계에 관한 연구)

  • Park, Chul-Woo;Kim, Young-Min;Kim, Chul;Kim, Young-Ho;Choi, Jae-Chan
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2002.05a
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    • pp.1012-1016
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    • 2002
  • This paper describes a research work of developing computer-aided design of a product with bending and piercing for progressive working. An approach to the system for progressive working is based on the knowledge-based rules. Knowledge for the system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system has been written in Auto-LISP on the Auto-CAD with a personal computer and is composed of four main modules, which are input and shape treatment, flat pattern layout, strip layout, and die layout modules. The system is designed by considering several factors, such as bending sequences by fuzzy set theory, complexities of blank geometry, punch profiles, and the availability of a press equipment. Strip layout drawing generated in the strip layout module is presented in 3-D graphic forms, including bending sequences and piercing processes with punch profiles divided into for external area. The die layout module carries out die design for each process obtained from the results of the strip layout. Results obtained using the modules enable the manufacturer for progressive working of electric products to be more efficient in this field.

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Development of a Process Sequence Determination Technique by Fuzzy Set Theory for Electric Product with Piercing and Bending Operations (퍼지셋을 이용한 퍼어싱 및 굽힘공정을 갖는 전기제품의 공정순서 결정기법 개발)

  • Kim J.H.;Kim Chul
    • Journal of the Korean Society for Precision Engineering
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    • v.22 no.9 s.174
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    • pp.137-146
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    • 2005
  • This paper describes a research work to develop a computer-aided design for the product made by progressive working of bending and piercing. An approach to the system for progressive working is based on the knowledge-based rules. Knowledge for the system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system has been written in AutoLISP on the AutoCAD with a personal computer and is composed of three main modules, which are input and shape treatment, flat pattern layout, strip layout modules. The system is designed by considering several factors, such as piercing and bending sequences by fuzzy set theory, complexities of blank geometry, punch profiles, and the availability of a press equipment. Strip layout drawing generated in the strip layout module is presented in 3-D graphic forms, including piercing and bending sequences with punch profiles divided into for external area. Results obtained using the modules enable the manufacturer for progressive working of electric products to be more efficient in this field.

A Study for Process Planning of Progressive Working by the using of Fuzzy Set Theory (Fuzzy set 이론을 이용한 프로그레시브 가공의 공정설계에 관한 연구)

  • Kim, Y. M.;Kim, J. H.;Kim, C.;Choi, J. C.
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2001.04a
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    • pp.735-739
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    • 2001
  • This paper describes a research work of developing computer-aided design of a product with bending and piercing for progressive working. An approach to the system for progressive working os based on the knowledge-based rules. Knowledge for the system is formulated from plasticity theorise, experimental results and the empirical knowledge of field experts. the system has been written in AutoLISP on the AutoCAD with a personal computer and is composed of three main modules, which are input and shape treatment, flat pattern layout and strip layout modules. Strip layout of the system is designed by using fuzzy set theory. Process planning is determinated by fuzzy value according to several rules. Strip layout drawing generated in strip layout module is presented in 3-D graphic forms, including bending sequences and piercing processes with punch profiles divided into for external area. Results obtained using the modules enable the manufacturer for progressive working of electric products to be more efficient in this field.

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Remodeling of Hull Form and Calculation of Design Parameters using Cubic Composite Spline (3차 복합 스플라인을 이용한 선형의 리모델링 및 설계 파라메터 계산)

  • Son, Hye-Jong;Kim, Hyun-Cheol
    • Korean Journal of Computational Design and Engineering
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    • v.13 no.6
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    • pp.440-449
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    • 2008
  • This paper deals with a method calculating various hull form parameters which are required in numerical analysis for ship performance such as motion, maneuverability, resistance and propulsion, etc. After the hull form is designed, before the model tests the ship's performances are evaluated by various analysis tools in which the hull form parameters are used with many kinds of forms aside from offset data. Here, The hull form parameters characterize the properties of hull form and contain positional, differential and integral information implicitly. Generally, the commercial CAD-system has not functions enough for supporting these form parameters and therefore each shipyard uses its own in-house analysis program as well as commercial analysis software. To overcome these limitations, modules for supporting these analysis programs have developed. The modules contain cubic composite spline cure using local curve fairing, intersect algorithm, Gaussian integral, and other geometric techniques needed in calculating hull form parameters. Using our analysis-supporting modules, a complex hull form can be remodeled exactly to the hull form designed by CAD-system and any hull form parameter required in various performance analyses can be calculated.

Design and implementation of IoT based controllers and communication module interfaces for stand-alone solar system

  • Lee, Yon-Sik;Mun, Young-Chae
    • Journal of the Korea Society of Computer and Information
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    • v.24 no.1
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    • pp.129-135
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    • 2019
  • This paper is part of research and development for stand-alone solar system without commercial power supply. It implements firmware of controller for operation of stand-alone solar system by applying IoT technology and also develops communication modules that allow multiple solar lamps to send and receive data through wireless network. The controller of the developed stand-alone solar system can effectively charge the power generated by the solar module, taking into account the battery's charge and discharge characteristics. It also has the advantage of attaching wireless communication modules to solar lamp posts to establish wireless communication networks without incurring communication costs. In addition, by establishing IoT gateway middleware platform for each installation site, it forms a foundation to operate multiple solar lamp posts into multiple clusters. And, it is expected that the data collected in each cluster will be used to enable configuration and control of operational information, thereby inducing convenience and efficiency of remote operation and management.

Implementing an Inflection Analyzer Program for English Verbs in a Word-and-Paradigm Morphology. (낱말.패러다임 형태이론에 입각한 영어동사 굴절 해석 프로그램의 구현)

  • No, Yong-Kyoon
    • Language and Information
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    • v.2 no.2
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    • pp.121-154
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    • 1998
  • The morphological analyzer is expected to tell attested word forms from imaginable yet unattested ones. An account of the inflectional morphology of English verbs is given in the framework of Word-and-Paradigm morphology, developed mainly by Matthews (1972, 1974, 1991) and further by Aronoff (1994) and Zwicky (1985, 1988), which is free of overrecognition. Thirteen inflectional classes are identified according to the patterns each of them exhibits in filling the slots in the paradigm. Peculiarity in orthography is also considered in assigning each verb lexeme to a class. Modules of a C program which gives associated morphosyntactic properties to all and only attested verb forms are written so that details of this framework can be evaluated explicitly. This program is shown to be superior to existing programs in economy and in the generality it achieves.

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Voice Activity Detection Algorithm using Wavelet Band Entropy Ensemble Analysis in Car Noisy Environments (프로세싱에서 삼각함수 공식을 응용한 장식적 타입페이스 제안)

  • Chun, Christine Hyeyeon
    • Journal of Korea Multimedia Society
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    • v.20 no.12
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    • pp.1992-1999
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    • 2017
  • This study proposes a decorative typeface which is produced through the concept of trigonometric functions in an open-source programming language known as Processing. First, the theoretical background of Processing and trigonometric functions as well as previous research in this area are analyzed. Second, basic modules of 'V', 'I', 'O', and 'M' were created for use as the final alphabet typeface with the concept of a trigonometric function. Third, a decorative parabolic curve that encircles the base module was created. Finally, the modules created on Processing were edited in Adobe Illustrator to create a typeface set with characters from A to Z. Various artworks using Programming can produce an infinite number of different versions by modifying only some of the variables and codes, and this method can include multimedia features such as text, images, videos, interactive art and various forms of content and media. Therefore, with regard to expression, the possibilities are endless. In this study, I attempt to expand the field of visual culture using programming and computational methodologies. In contrast to the digital typeface production method, which relies on existing graphic tools, this study is meaningful because it expands the range of use of decorative typefaces.

The New Smart Power Modules for up to 1kW Motor Drive Application

  • Kwon, Tae-Sung;Yong, Sung-Il
    • Journal of Power Electronics
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    • v.9 no.3
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    • pp.464-471
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    • 2009
  • This paper introduces a new Motion-$SPM^{TM}$ (Smart Power Modules) module in Single In-line Package (SIP), which is a fully optimized intelligent integrated IGBT inverter module for up to 1kW low power motor drive applications. This module offers a sophisticated, integrated solution and tremendous design flexibility. It also takes advantage of pliability for the arrangement of heat-sink due to two types of lead forms. It comes to be realized by employing non-punch-through (NPT) IGBT with a fast recovery diode and highly integrated building block, which features built-in HVICs and a gate driver that offers more simplicity and compactness leading to reduced costs and high reliability of the entire system. This module also provides technical advantages such as the optimized cost effective thermal performances through IMS (Insulated Metal Substrate), the high latch immunity. This paper provides an overall description of the Motion-$SPM^{TM}$ in SIP as well as actual application issues such as electrical characteristics, thermal performance, circuit configurations and power ratings.