• Mycobiology
• /
• 제31권4호
• /
• pp.226-228
• /
• 2003

The multiple correlation coefficient between the values determined by dry weight and those determined by fluorometer was observed with r=0.96 and the standard error of calibration was 0.034. Using the best calibration data, in order to reconfirm the reliability of the fluorometer results in comparison with those obtained by dry weight on the cell concentration, fedbatch cultures were carried out. The results obtained by fluorometer measurements were in good agreement with those obtained by dry weight. The on-line monitoring of cell concentration by the fermentor system linked to a computer equipped with fluorometer was successfully carried out.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
• /
• pp.1-3
• /
• 2003

In this study, spectroradiometer data were coupled with fluorometer data to find out the best suited bands ratio to monitor the chlorophyll a concentration for inland water. Remote sensing reflectance measurements were used to evaluate the performance of several default ocean color chlorophyll algorithms for SeaWiFS data. This study shows that the chlorophyll a concentration from fluorometer and reflectance from spectroradiometer lies in exploiting the signal provided by the chlorophyll a red absorption peak near 670nm. Two-band ratio based on a ratio of reflectance 670 and 700nm provided a good correlation for a linear model, compare with blue-green two band ratio.

• 한국환경보건학회지
• /
• 제34권6호
• /
• pp.423-432
• /
• 2008

In this study, we investigated the possibility of auto control and the proper operating factors in the BNR(Biological Nutrient Removal) process using an NADH(Reduced Nicotinamide Adenine Dinucleotide) fluorometer, which characterized the emitted fluorescence when activated by flashes of UV light at 460 nm. In terms of finding adequate operating parameters, results indicted that nitrification efficiency decreased in the controlled DO while denitrification efficiency decreased in the controlled pH. The above results indicated that controlled operating condition after combination with NADH, DO and pH was resonable. Result obtained from the correlation between NADH and pH showed that variation trend of influent loading was similar to those of NADH and pH, and also the variation cycle was repeated on a daily basis. Consequently, this result showed the increase of BOD loading caused the nitrification efficiency to decrease because air-flow, required for nitrification, was reduced, and so the NADH value was increased. From these results, it is possible to use NADH flourimetry to assess the variation of organic load and nitrification efficiency in the case of small change in influent pH such as in sewage and also to handle and operate the load variation in the auto control system using the NADH fluorometer.

• 한국대기환경학회:학술대회논문집
• /
• 한국대기환경학회 2000년도 춘계학술대회 논문집
• /
• pp.110-111
• /
• 2000

대기중 가스상 $H_2O_2$(Hydrogen Peroxide)는 액상 화학반응과 기상 라디칼반응사이에 연결고리의 역할을 할 뿐만 아니라, 대기중의 $SO_2$를 $H_2SO_4$로 산화시키는 산화제로서 구름, 안개, 이슬 및 빗물의 산화에 중요한 역할을 담당한다. 또한 가스상 $H_2O_2$는 연쇄종결자와 $HO_2$.(hydroperoxyl radical)농도의 지표로서 광화학 스모그에 있어 중요한 화학종이기도 하다. $H_2O_2$농도의 증가는 결국 대기의 산화율 및 속도를 증가시키고 대류권내의 액상중에서 $H_2SO_4$ 생성을 가속화시킨다는 것은 이미 잘 알려져 있는 사실이다. (중략)

• 대한화학회지
• /
• 제56권5호
• /
• pp.577-582
• /
• 2012

Quinine quantitative analysis, photo-bleaching, fluorescence quenching, and urine analysis have been performed by means of a UV-LED fluorometer, which can be easily built and used in a high-school laboratory. The quinine detection range is estimated to be 0.05-80 ppm, enough for many classroom luminescence experiments. The quinine content in commercial tonic water is determined from the calibration curve, and UV photo-bleaching of this anti-malarial drug is demonstrated with clear wavelength dependence. Halide quenching of quinine fluorescence is also observed and the increase in quenching efficiency in the order of $Cl^-$, $Br^-$, and $I^-$ is evident. Urine analyses for the student volunteers have been carried out and the results clearly reveal excretion of the ingested quinine. The student participants are exuberant throughout the course of this study and sense the practices resourceful.

• KSBB Journal
• /
• 제21권5호
• /
• pp.341-346
• /
• 2006

본 연구에서는 sonificator를 장착하여 세포막을 파쇄하고 현장에서 형광을 이용하여 조작이 간편하고 단시간에 DNA를 측정할 수 있는 자동화된 형광기를 개발하기 위하여 그람 음성균인 Escherchia coli를 대상으로 최적의 세포 파쇄조건을 확립하고자 하였다. Incubation time은 형광량에 크게 영향을 주지 못하는 것으로 나타났으며, 가열처리 방법은 현장에서 세포를 파괴하는 방법으로는 파쇄효과가 미약하고 적합하지 않은 것으로 나타났다. Sonificator probe 직경에 따라 세포의 파쇄 효과가 큰 차이를 보였으며 13 mm probe로 20초 동안 sonification시키는 것이 가장 효율적인 것으로 나타났다. 시료에 잠긴 Sonificator probe tip 깊이에 따라서도 세포의 파쇄 효과가 크게 나타났는데 시료에 잠긴 probe tip의 깊이가 깊을수록 큰 파쇄 효과를 발휘하였다. 선정된 최적의 파쇄 조건에서 $5{\times}10^5CFU/m{\ell}$의 Escherchia coli를 탐지 가능한 것으로 나타났다.

• KSBB Journal
• /
• 제21권5호
• /
• pp.347-352
• /
• 2006

본 연구에서는 sonificator를 장착하여 세포막을 파쇄하고 현장에서 형광을 이용하여 조작이 간편하고 단시간에 DNA를 측정할 수 있는 자동화된 형광기를 개발하기 위하여 그람 양성균으로 간균인 Bacillus globigii와 구균인 Streptococcus epidermidis를 대상으로 최적의 세포 파쇄조건을 확립하고자 하였다. Sonificator probe 직경에 따라 세포의 파쇄 효과가 큰 차이를 보였으며 13 mm probe로 20초동안 sonification시키는 것이 가장 효율적인 것으로 나타났으며 간균인 Bacillus globigii가 구균인 Streptococcus epidermidis보다 더 파쇄가 잘 되는 것으로 나타났다. 시료에 잠긴 Sonificator probe tip 깊이에 따라서도 세포의 파쇄 효과가 크게 나타났는데 시료에 잠긴 probe tip의 깊이가 깊을수록 큰 파쇄 효과를 발휘하였다. 선정된 최적의 파쇄 조건에서 최저 탐지농도는 Bacillus globigii, Streptococcus epidermidis 모두 $5{\times}10^5CFU/m{\ell}$의 농도를 탐지 가능한 것으로 나타났다.

• 한국환경과학회지
• /
• 제25권3호
• /
• pp.439-446
• /
• 2016

Domestic sewerage treatment plant is operated by activated sludge method and its modified method by using microorganism. In most cases, a method of using microorganism is directly controlled by the operator based on individual judgment through factors of DO, pH and ORP. In addition, under aerobic condition in bioreactor, energy consumption including excessive air injection is learned to be somewhat plenty. In order to solve this problem, in most of the process, improvement of internal recycling and activated environmental factor of microorganism were researched extensively. However, as factors are changed depending on various conditions, it is not sufficient as an indicator of judgment. As such, a research on operation of bioreactor that measures metabolic change in short time by directly measuring activated condition of microorganism using NADH fluorometer is required in reality.It is considered that the method like this could supplement problem of energy consumption being occurred in the existing treatment method and operational optimization of bioreactor would be enabled by controlling optimal air volume. Therefore, in this study, in order to obtain optimal operational indicator of bioreactor, proper air volume control test was performed and through batch test and site evaluation, possibility of NADH sensor being utilized as operational control indicator of bioreactor is intended to be analyzed. In order to compare with measured value, DO, ORP that are operational control indicator of existing bioreactor were used. As MLSS concentration was increased through batch test, NADH value was increased and site evaluation also showed similar tendency to batch test. Resultantly, it could be confirmed that changing level of NADH fluorometer was a sensor that could measure bioreactor condition effectively and optimized scale of bioreactor is considered to be utilized.

• 한국수산과학회지
• /
• 제20권3호
• /
• pp.219-223
• /
• 1987

초산연법 어육중의 riboflavin 함량측정에 적용가능한지를 검토하기 위하여 riboflavin 함량과 형광강도 상관성을 검량곡선으로 비교검토하였으며, 그 회귀식을 구하였다. 1. 초산연법은 개량 AOAC법에 비하여 정확도가 높고 실험방법이 간단 신속하므로 실험상의 오차를 줄일 수 있어 어육중의 riboflavin 함량측정에 적용할 수 있는 방법일 것으로 판단된다. 2. 백색어육류의 riboflavin 함량을 초산연안법으로 측정하였은 때 riboflavin 함량과 형광강도와의 상관식은 y=129.70x+0.71 이었다. 3. 9종의 백색육어류의 riboflavin 함량은 생식료 100g 중 $0.29\~0.48mg$ 4. 말쥐치육을 18일간 빙장한 결과, riboflavin의 함량변화는 미미하였다.

• ALGAE
• /
• 제25권2호
• /
• pp.89-97
• /
• 2010

To test the feasibility of using chlorophyll ${\alpha}$ fluorescence to assess the establishment success of seagrass transplants, photosynthetic characteristics of eelgrass Zostera marina transplants were measured using a Diving-pulsed amplitude modulation fluorometer in Jindong Bay on the southern coast of Korea. Maximum quantum yield ($F_v/F_m$), photosynthetic efficiency ($\alpha$), saturating irradiance ($E_k$) and maximum electron transport rate ($ETR_{max}$) of transplants and reference plants in a nearby transplant site were measured using the fluorometer for 5 months. Additionally, shoot morphology, individual shoot weight and productivity of transplants and reference plants were also monitored. Shoot height, leaf weight and productivity of transplants were significantly reduced during the first two or three months after transplantation compared to those of reference plants, and then increased to the levels of reference plants Characteristics of chlorophyll a fluorescence, including $F_v/F_m$, $\alpha$, $E_k$ and $ETR_{max}$ of transplants were also significantly reduced in the initial period, but recovered slightly sooner than shoot morphology or leaf productivity. These results indicated that after transplantation, Z. marina transplant photosynthesis recovered faster than shoot morphology, biomass or productivity. Thus, chlorophyll a fluorescence can be used as an indicator for early assessment of the status of eelgrass transplants without destructive sampling.