Determination of Riboflavin Content in Fishes

어육중의 riboflavin 함량의 측정

A slightly modified method of Rashid and Potts(1980) to determine riboflavin in milk in which lead acetate was used as a precipitant was employed in tile present study to test applicability to determine that of fish as well. The lead acetate method was found to be sensitive, simple, inexpensive and rapid compared to the modified A.O.A.C. method by Cordon et al. (1979). But higher riboflavin values were obtained in this study than those reported so far. The riboflavin contents of 9 white fleshed fishes were in the range of $0.29\~0.48mg$ per 100g fresh sample. Linear regression equation Y=125.70X+0.71 (R=0.9993) was obtained for the calculation of riboflavin content in the white fleshed fish. Y is the concentration of riboflavin in the final solution to be chocked its OD at fluorometer and X is the dial reading of fluorometer. The stability of riboflavin as the freshness changes during icing storage$(at\;0^{\circ}C)$ was studied with file fish. During the initial stage of storage, the riboflavin content was found to by increased by $14\%$, but the difference was not statistically significant (p>0.05). K-value and VBN-value were increased along with storage time, but Proximate composition was not changed significantly during entire storage of 18 days.

초산연법 어육중의 riboflavin 함량측정에 적용가능한지를 검토하기 위하여 riboflavin 함량과 형광강도 상관성을 검량곡선으로 비교검토하였으며, 그 회귀식을 구하였다. 1. 초산연법은 개량 AOAC법에 비하여 정확도가 높고 실험방법이 간단 신속하므로 실험상의 오차를 줄일 수 있어 어육중의 riboflavin 함량측정에 적용할 수 있는 방법일 것으로 판단된다. 2. 백색어육류의 riboflavin 함량을 초산연안법으로 측정하였은 때 riboflavin 함량과 형광강도와의 상관식은 y=129.70x+0.71 이었다. 3. 9종의 백색육어류의 riboflavin 함량은 생식료 100g 중 $0.29\~0.48mg$ 4. 말쥐치육을 18일간 빙장한 결과, riboflavin의 함량변화는 미미하였다.