• Title/Summary/Keyword: fluorescent

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Fluorescent Compounds Having the Spaced and Integrated Type Receptors

  • Choi, Chang-Shik
    • Rapid Communication in Photoscience
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    • v.5 no.1
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    • pp.1-7
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    • 2016
  • Fluorescent receptors have gained much attention because of their usefulness in analysis and clarification of the roles of biomolecules in living systems. Molecular structures of the integrated type including that the receptor itself is fluorescent, and play an important role in having the functionality or selectivity of the fluorescent compounds. These spaced type fluorescent receptors are required to have special molecular design in order to transmit the information of molecular recognition to the fluorescent unit through the spacer unit. Compared with the spaced type fluorescent receptors, number of the integrated type receptors is limited due to the difficult molecular design and synthesis. Modification of alteration of the fluorophore frequently caused deterioration of the fluorescent property. Various spaced type and integrated type fluorescent receptors including switch on-off receptors are introduced in this article.

Expression of various fluorescent protein and their production in shake flasks

  • Park, So-Jung;Han, Kyung-Ah;Rhee, Jong-Il
    • 한국생물공학회:학술대회논문집
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    • 2005.04a
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    • pp.408-411
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    • 2005
  • The green fluorescent protein (GFP) from the jellyfish aequorea and its fluorescent homologs from Anthozoa corals have become invaluable tools for imaging of cells and tissues. In this study various fluorescent protein such as green fluorescent protein (GFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP) have been expressed in Escherichia coli. Growth of recombinant cells and production of fluorescent proteins were investigated in shake flasks. Some characteristics of fluorescent proteins was also studied.

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Fluorescent characteristics of baits and bait cages for swimming crab Portunus trituberculatus pots (꽃게 통발용 미끼 및 미끼통의 형광 특성)

  • Chang, Ho-Young;Koo, Jae-Geun;Lee, Keun-Woo;Cho, Bong-Kon;Jeong, Byung-Gon
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.44 no.3
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    • pp.174-183
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    • 2008
  • In order to develop the substitutive materials for natural baits of swimming crab pots, the fluorescent characteristics of the baits were analyzed, and the preference of fluorescent dyes were investigated by the mean entrapped catch number to the pots through the water tank experiments and fishing experiments. On the investigation of fluorescent characteristics by the 5 kinds of baits, mackerel, krill, manila clam, pig's fat and chicken's head which were used in substitutive baits for test in the UV long wave(365nm) area, it showed clear blue fluorescence in the skin of mackerel, shell of krill, manila clam and bill of chicken's head, and green fluorescence in the mackerel s muscle and internals, and yellow fluorescence in the pig's fat and chicken's head. On the investigation of fluorescent characteristics by the bait cages in the UV short wave(254nm) and long wave(365nm) area, it showed each green, red and blue fluorescence in the cylinderical or hexahedral red plastic bait cages which were painted each green, red and blue fluorescence dyes, but it showed yellowish green flourescence in the cylinderical or hexahedral red plastic bait cage which was painted yellow fluorescent dye. On the preference investigation of the fluorescent dyes of swimming crabs by the 5 kinds of the bait cages which were put the mackerel in the non-fluorescent red plastic cage($RF_N$), red, yellow, green and blue fluorescent plastic cages(RF, YF, GF, BF) each, nonfluorescent red plastic cage($RF_N$) was entrapped mean 2.0(6.7%), but blue fluorescent plastic cage(BF) was mean 5.0(16.7%) and it was more 2.5 times comparing to $RF_N$, and red fluorescent cage(RF) was same level and green fluorescent cage(GF) was 50% of catch number comparing to $RF_N$, and yellow fluorescent cage(YF) was entrapped nothing(F 46.324, P < 0.05). On the investigation of the entrapped catch number to the pots which were put the mackerel in the blue fluorescent hexahedral plastic cage(HP) and blue fluorescent silicon mackerel model cage(SM), HP was mean 3.4(11.3%) and it was a little more comparing to SM which was entrapped mean 3.2(10.7%)(t 0.775, P > 0.05). Fishing experiments on the preference investigation of swimming crabs by the pots which were put in the non-fluorescent red plastic cage($RF_N$) and blue fluorescent plastic cage(BF) were conducted 3 times. Mean catching number and weight of $RF_N$ were 71.7 ind.(18.3%) and 16.9kg(64.3%), and those of BF were 93.0 ind.(23.1%) and 19.8kg(64.5%), respectively.

Development of The LED FDL for Replacing Fluorescent Lamp (형광램프대체용 LED FDL 개발)

  • Yang, Byong-Moon;Jang, Woojin;Cha, Jae-Sang
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.29 no.1
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    • pp.9-14
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    • 2015
  • Due to the declining LED price and environment-friendly energy policies, CO2 emission reduction and energy-saving, the LED lighting industry is accelerating rapidly. In particular, the needs for LED lamp, replacing the existing fluorescent lamp without exchange luminaire or driver circuits, are also rapidly increasing. Therefore, replacement for T8 fluorescent lamp, LED T8 lamp was developed and standardized. However, except LED T8 lamps, other lamps' regulations or standards were not enacted. Also, the stability of fluorescent lamp substitutable LED lamp is low due to the difference in electrical characteristics and mismatch between the existing fluorescent lamp ballast and LED lamp. Therefore, many are struggling while developing fluorescent lamp substitutable LED lamp. In this paper describes the properties of existing fluorescent lamp ballasts and the considerations while developing fluorescent lamp substitutable LED lamp : demonstrating its validity by experiments the developed fluorescent lamp substitutable LED lamp prototype.

The Recent Current of Fluorescent Polypyridine Compounds Having Photofuctionality

  • Choi, Chang-Shik
    • Rapid Communication in Photoscience
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    • v.5 no.2
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    • pp.21-26
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    • 2016
  • Many kinds of fluorescent polypyridine compounds including bpy and dppz derivatives are described in understanding the recent current of fluorescent materials having photofuctionality. Those polypyridine compounds have the photofunctionality such as the fluorescence recognition and/or photo-switching. Furthermore, those compounds are applicated for the construction of long ranged photoinduced electron/energy transfer system. Various fluorescent ${\pi}-conjugation$ systems connected by amide or imine bond as well as the simple fluorescent bpy derivatives are introduced in this review paper.

Growth Responses and Introduction Plan of Interior Landscape Plants under Light Intensity of Fluorescent Light and Sunlight (형광등과 자연광의 광도에 따른 실내조경식물의 생육반응과 도입 방안)

  • Choi Kyoung-Ok;Lee Sang-Woo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.33 no.4 s.111
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    • pp.119-128
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    • 2005
  • This study was carried out to obtain fundamental information of growth response of interior landscape plants under a fluorescent lighting, a sunlight at indoor. Ficus elastica Roxb. vu. decora Hort, Spathiphyllum 'Clevelandii', Codiaeum variegatum Blume var. pictum Mvell. Arg. 'Exalant' and Cordyline terminalis Kunth var. red edge Hort were examined under 100lux, 500lux, 1,000lux and 2,000lux light intensity consisted of fluorescent lighting and sunlight at indoor condition. Result of experiments are as follows; 1. A Ficus elastica Roxb. var. decora Hort plant growth status was better showed under fluorescent lighting than sunlight. A plant growth status showed the best result under 2,000lux light intensity of fluorescent lighting in cases of all conditions. 2. A Spathiphyllum 'Clevelandii' showed the best effective adaptations under law intensity among experimental plants. A Spathiphyllum 'Clevelandii', plant growth status was better showed under fluorescent lighting than sunlight. A plant growth status showed the best result under 1,000lux light intensity of fluorescent lighting in cases of all conditions. 3. It need the best high establishment of fluorescent lighting among experimental plants for good plant growth. A Codiaeum variegatum Blume var. pictum Mvell. Arg. 'Exalant' plant growth status was better showed under fluorescent lighting than sunlight. A plant growth status was better showed under high light intensity in case of same light source. A plant growth status showed the best result under 2,000lux light intensity of fluorescent lighting. 4. A Cordyline terminalis Kunth var. red edge Hort plant growth status was better showed under fluorescent lighting than sunlight. but, A plant growth status was better showed under $500lux\sim1,000lux$ than 2,000lux in cases of all intensity of fluorescent lighting. while, A plant growth status was better showed under $1,000lux\sim2,000lux$ in cases of all intensity of sunlight.

Effect of degumming conditions on the fluorescence intensity of fluorescent silk cocoons: A combined experimental and molecular dynamics study

  • Chan Yeong, Yu;Ezekiel Edward, Nettey-Oppong;Elijah, Effah;Su Min, Han;Seong-Wan, Kim;Seung Ho, Choi
    • International Journal of Industrial Entomology and Biomaterials
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    • v.45 no.2
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    • pp.56-69
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    • 2022
  • Silk is a unique natural biopolymer with outstanding biocompatibility, high mechanical strength, and superior optical transparency. Due to its excellent properties, silk has been widely reported as an ideal biomaterial for several biomedical applications. Recently, fluorescent silk protein, a variant of native silk, has been reported as a biophotonic material with the potential for bioimaging and biosensing. Despite the realization of fluorescent silk, the traditional degumming process of fluorescence silk is crude and often results in fluorescence loss. The loss of fluorescent properties is attributed to the sensitivity of silk fibroin to temperature and solvent concentration during degumming. However, there is no comprehensive information on the influence of these processing parameters on fluorescence evolution and decay during fluorescent silk processing. Therefore, we conducted a spectroscopic study on fluorescence decay as a function of temperature, concentration, and duration for fluorescent silk cocoon degumming. Sodium carbonate solution was tested for degumming the fluorescent silk cocoons with different concentrations and temperatures; also, sodium carbonate solution is combined with Alcalase enzyme and triton x-100 to find optimal degumming conditions. Additionally, we conducted a molecular dynamics study to investigate the fundamental effect of temperature on the stability of the fluorescent protein. We observed degumming temperature as the prime source of fluorescent intensity reduction. From the MD study, fluorescence degradation originated from the thermal agitation of fluorescent protein Cα atoms and fluctuations of amino acid residues located in the chromophore region. Overall, degumming fluorescent silk with sodium carbonate and Alcalase enzyme solution at 25 ℃ preserved fluorescence.

Evaluation of Cell Viability and Delivery Efficiency in Electroporation System According to the Concentrations of Propidium Iodide and Yo-Pro-1 (전기천공시스템에서 Propidium Iodide와 Yo-Pro-1의 농도에 따른 세포 생존율과 전달효율 평가)

  • Bae, Seo Jun;Im, Do Jin
    • Korean Chemical Engineering Research
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    • v.57 no.6
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    • pp.898-906
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    • 2019
  • In this study, basic research was conducted to provide guidelines for selecting fluorescent dye and using proper concentration of fluorescent dye to use evaluation of cell viability and fluorescent dye delivery efficiency. Propidium iodide and Yo-Pro-1 were used as fluorescent dyes. In the evaluation of cell viability and the efficiency of delivery using Propidium Iodide and Yo-Pro-1, the histogram of each fluorescent dye was different depending on the type of fluorescent dye and the concentration used. These results were related to the characteristics of the fluorescent dyes used. This was related to the properties of the fluorescent dyes used. From these results, it was found that the analytical results depending on the characteristics of the fluorescent dyes used in the cell analysis. The effect of the fluorescent dye on the cell was confirmed, but it was confirmed that it did not affect the analysis result. In addition, the influence of interference between fluorescent signals was confirmed when two or more kinds of fluorescent dyes were used for analysis. The higher the concentration of Yo-Pro-1 was, the larger the effect of interference was, and the concentration of Propidium Iodide did not affect the interference of fluorescence signal. This study confirmed that the evaluation of the cell viability and the evaluation of the delivery efficiency were influenced by the type and concentration of the fluorescent dyes and it was related to the characteristics of the fluorescent dyes. Based on the results, appropriate concentrations of fluorescent dyes suitable for evaluation of cell viability and delivery efficiency were suggested.

Fluorescent Pattern Generation on the Fluorescent Photopolymer with 2-beam Coupling Method (2-beam Coupling 방법을 이용한 광 고분자 형광 패턴 형성)

  • Kim, Yoon-Jung;Kim, Jeong-Hun;Sim, Bo-Yeon;Lee, Myeong-Kyu;Kim, Eun-Kyoung
    • Korean Journal of Optics and Photonics
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    • v.21 no.1
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    • pp.6-11
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    • 2010
  • Fluorescent photopolymer film was prepared with composition containing acrylate monomer, binder, a visible light sensitive photo initiator, and fluorescent anthracene polymer. A fluorescent grating pattern was inscribed on the photopolymer film using a 2-beam coupling method. A 514 nm laser was coupled to generate a beam-interference pattern. A highly fluorescent diffractive line pattern was formed on the fluorescent photopolymer within 30 sec. of exposure. The fluorescence intensity was highly enhanced in the patterned area, possibly due to the change in the environment of the fluorescent polymers by the photo-polymerization of monomers. Under a photo-mask, a gap electrode pattern was formed of fluorescent gratings with a sub-micron scale, which was matched well to the calculated value ($2.5\;{\mu}m$ and $0.6\;{\mu}m$) based on the refractive index of the photopolymer and beam incident angle ($3.4^{\circ}$, $15^{\circ}$) to the photopolymer surface.

Fluorescent Nanoparticles: Synthesis and Applications (형광 나노입자: 합성 및 응용)

  • Kim, Y.K.;Song, B.K.;Lee, J.G.;Baek, Y.K.
    • Journal of Powder Materials
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    • v.27 no.2
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    • pp.154-163
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    • 2020
  • Fluorescent nanoparticles are characterized by their unique properties such as luminescence, optical transparency, and sensitivity to various chemical environments. For example, semiconductor nanocrystals (quantum dots), which are nanophosphors doped with transition metal or rare earth ions, can be classified as fluorescent nanoparticles. Tuning their optical and physico-chemical properties can be carried out by considering and taking advantage of nanoscale effects. For instance, quantum confinement causes a much higher fluorescence with nanoparticles than with their bulk counterparts. Recently, various types of fluorescent nanoparticles have been synthesized to extend their applications to other fields. In this study, State-of-the-art fluorescent nanoparticles are reviewed with emphasis on their analytical and anti-counterfeiting applications and synthesis processes. Moreover, the fundamental principles behind the exceptional properties of fluorescent nanoparticles are discussed.