• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.622-629
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• 2018

Expansins are cell wall proteins that mediate cell wall loosening and promote specific tissue and organ morphogenesis in plants and in some microorganisms. Unlike plant expansins, the biological functions of fungal expansin-like proteins have rarely been discussed. In the present study, an expansin-like protein-encoding fvexpl1 gene, was identified from Flammulina velutipes by using local BLAST. It consisted of five exons with a total length of 822 bp. The deduced protein FVEXPL1 contained 274 amino acids with a predicted molecular mass and isoelectric point of 28,589 Da and pH 4.93, respectively. The first 19 amino acids from the N terminal are the signal peptide. Phylogenetic analysis and multiple protein alignment indicated FVEXPL1 was an expansin-like protein. The expression level of fvexpl1 gene in the stipe was significantly higher than that in the mycelia, primordia, and cap. However, the expression level of fvexpl1 gene was significantly higher in the fast elongation region of the stipe as compared with the slow elongation region. Expression analysis indicated that fvexpl1 gene might have an auxiliary role in the stipe morphogenesis of F. velutipes.

• Mycobiology
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• 제47권4호
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• pp.441-448
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• 2019

Two new SAM-dependent methyltransferase encoding genes (fvsmt1 and fvsmt2) were identified from the genome of Flammulina velutipes. In order to make a comprehensive characterization of both genes, we performed in silico analysis of both genes and used qRT-PCR to reveal their expression patterns during the development of F. velutipes. There are 4 and 6 exons with total length of 693 and 978 bp in fvsmt2 and fvsmt1, respectively. The deduced proteins, i.e., FVSMT1 and FVSMT2 contained 325 and 230 amino acids with molecular weight 36297 and 24894 Da, respectively. Both proteins contained a SAM-dependent catalytic domain with signature motifs (I, p-I, II, and III) defining the SAM fold. SAM-dependent catalytic domain is located either in the middle or at the N-terminal of FVSMT2 and FVSMT1, respectively. Alignment and phylogenic analysis showed that FVSMT1 is a homolog to a protein-arginine omega-N-methyltransferase, while FVSMT2 is of cinnamoyl CoA O-methyltransferase type and predicted subcellular locations of these proteins are mitochondria and cytoplasm, respectively. qRT-PCR showed that fvsmt1 and fvsmt2 expression was regulated in different developmental stages. The maximum expression levels of fvsmt1 and fvsmt2 were observed in stipe elongation, while no difference was found in mycelium and pileus. These results positively demonstrate that both the methyltransferase encoding genes are involved in the stipe elongation of F. velutipes.

• 농업생명과학연구
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• 제44권6호
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• pp.141-146
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• 2010

본 연구에서는 국내에서 식용으로 재배되고 있는 팽이버섯의 균사체에 대하여 Agrobacterium을 이용한 형질전환을 시도하였다. 특히 물리적 연마제인 미세 aluminum oxide 입자를 팽이 균사체와 함께 강하게 교반함으로써 물리적 상해를 지니는 균사체를 제조하였으며 감압침윤에 의한 Agrobacterium 이용 형질전환을 시도하였다. Hygromycin 저항성을 이용한 선발 결과, 대조군에서는 균사체 생육이 전혀 관찰되지 않은 반면 물리적 상해군에서는 형질전환균사체 생육이 확인되었다. Genomic DNA PCR에 의한 유전자 도입을 확인함으로써 팽이균사체에 대한 매우 간편한 형질전환법을 제시할 수 있었다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 한국생물과학협회 학술발표대회
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• pp.240.1-240
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• 1999

No Abstract, See Full Text

• 한국균학회지
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• 제15권1호
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• pp.38-41
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• 1987

Neohaplonts were obtained to improve mushroom strain from dikaryon strains of Flammulina Velutips, Ganoderma lucidum, Lyophyllum ulmarium, Pleurotus cornucopiae and Pleurotus spodoleucus by protoplast reversion. The noehaplont frequency from reversion colonies of protoplasts was 4.47-47.7%. Four more types of morphological characteristics were detected from neohaplonts of protoplast reversion in L. ulmarium, P. cornucopiae and P. spodoleucus, but one or two types were neohaplonts in F.velutipes and G. lucidum. Growth rate of neohaplonts was slow compared with dikaryon strains.

• 한국버섯학회지
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• 제14권1호
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• pp.21-26
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• 2016

The mannanase-producing bacteria, designated YJ17, was isolated from spent mushroom (Flammulina velutipes) substrates. The isolate YJ17 was a facultative anaerobic and was grown at temperatures ranging from $20^{\circ}C$ to $50^{\circ}C$ with an optimal temperature of $40^{\circ}C$. The DNA G+C content of the YJ17 was 44 mol%. The major fatty acids were anteiso-15:0 (38.9%), 17:0 (7.6%), and iso-15:0 (36.5%). The 16S rRNA gene sequence similarity between the isolate YJ17 and other Bacillus strains was from 98% to 99%. In the phylogenetic analysis based on these sequences, the isolate YJ17 and Bacillus amyloliquefaciens clustered within a group together and separated from other species of Bacillus. Based on the physiological and molecular properties, the isolate YJ17 was classified within the genus Bacillus as B. amyloliquefaciens YJ17. The optimal pH and temperature for mannanase activity of B. amyloliquefaciens YJ17 were pH 7.0 and $50^{\circ}C$, respectively.

• Nutrition Research and Practice
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• 제1권3호
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• pp.180-183
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• 2007

Cytoprotective ability of polysaccharides isolated from different edible mushrooms was investigated on the 7-ketocholesterol-induced damaged cell line. Polysaccharide extracts from six different edible mushrooms-Flammulina velutipes, Peurotus ostreatus, Lentinus edodes, Agrocybe aegerita, Agaricus blazei, and Cordyceps militaris- were prepared by hot water extraction and alcohol precipitation. Cytoprotective ability was evaluated by measuring the viable cells of the normal embryonic liver cell line (BNL CL. 2) in the presence of 7-ketocholesterol. At $80\;{\mu}g/mL$ of 7-ketocholesterol, cytotoxicity was very high with a loss of 98% of viable cells after 20 h of incubation. With the addition of $200\;{\mu}g/mL$ of each polysaccharide isolate to the cell line containing $80\;{\mu}g/mL$ of 7-ketocholesterol, polysaccharide isolates from both Flammulina velutipes and Peurotus ostreatus could significantly inhibit the 7-ketochoelsterol-induced cytotoxicity in the cells. But other polysaccharide isolates were not effective in inhibiting cell damage caused by the oxLDL-induced cytotoxicity.

• 생명과학회지
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• 제11권6호
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• pp.537-542
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• 2001

몇몇 식용 및 약용식물의 버섯의 천연 생리활성성분을 기능성 식품 및 의약품 원료로 사용할 목적으로 이들의 ${\gamma}$-aminobutyric acid 함량을 축정하여 그 기초자료를 제공하는데 있다. 본 실험에서 사용된 자원식물중에서 뽕나무잎 및 꾸지뽕나무잎ㅍ과 열매에서 각각 134.03, 104.13및 120.99 mg/100g gDM로 많이 함유되어 있었다. 또한 버섯유에서 ${\gamma}$-aminobutyric acid의 함량은 식용버섯인 느타리버섯, 팽이버섯, 및 표고버섯에서 각각 93.44m 72.17 및 61.15 mg/100g DM로 약용 버석인 상황버석과영지버석의 10.31및 3.09 mg/100 g DM보다 높게 나타났다. 이상의 결과에서 약용자원식물인 뽕나무류와 식용버섯류에서 천연 생리활성성분인 ${\gamma}$-aminobutyric acid를 비교적 많이 함유하고 있어 가능성 식품의 의약품원료로 사용될 가능성이 제기되었다.

• Food Quality and Culture
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• 제3권1호
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• pp.6-10
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• 2009

This study examined the effects of adding Pleurotus ostreatus, Agaricus bisporus and Flammulina velutipes on the quality characteristics of cookies and multiple purposes of wheat powders. The water content, crude protein content, mineral content, color value and sensory evaluation of mushroom cookies and multiple purposes of wheat powders were measured. In the case of cookies, the water content was 1.10-1.24% and crude protein content was 8.20-9.80%. General preferences, such as appearance, color, taste and texture, increased when mushroom was added to the cookies, especially flavor, which showed a much higher preference. In the studies where mushroom was added to wheat powders, the water content was 0.57-0.92% and crude protein content was 11.90-12.80%. The addition of mushroom to both of the cookies and multiple purposes of wheat powders resulted in a remarkable increase in the mineral content, especially Fe, K. In addition, an increase in the amount of added mushrooms resulted in an increase in the hunter L and a values; however, this also resulted in a decrease in the b value.

• 한국식품과학회지
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• 제13권4호
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• pp.255-260
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• 1981

비타민과 무기염류가 팽나무 버섯균(菌)의 균사생육(菌絲生育)과 자실체형성(字實體形成) 미치는 영향에 대하여 실험하였다. 비타민 중에서는 티아민이 제일 우수하였고 그 최적 농도는 $50{\mu}g%$이었으며, 무기 염류 중에서 $KH_{2}PO_{4}$의 최적농도는 0.2%, $MgSO_{4}$의 최적농도는 0.02%이었으며 그 밖의 무기염류를 단용 또는 혼용시에 균사(菌絲)와 자실체(字實體)의 수율에 효과가 없었다.