• Title/Summary/Keyword: fixative

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Fixation of $\gamma$-Radionuclides by the PVA-PMAA System

  • Won, Hui-Jun;Ahn, Byung-Gil;Jung, Chong-Hun;Kim, Gye-Nam;Oh, Won-Zin
    • Nuclear Engineering and Technology
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    • v.32 no.3
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    • pp.205-213
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    • 2000
  • A series of laboratory experiments was conducted to investigate the fixation characteristics of poly(vinylalcohol)-poly(methacrylic acid)(PVA-PMAA) mixed solution on the soluble ${\gamma}$- radionuclides. Using the potentiometric titration technique, it was found out that the PVA and PMAA in a solution form intermacromolecular complex. The mobilized portion of each radionuclide by water from sand surface treated with a fixative was measured by ${\gamma}$-ray spectroscopy. The mobilized portion of minor radionuclides such as 241Am, 154Eu, 155Eu and 144Ce were higher than those of 134Cs and 137Cs. The capability of PVA-PMAA system was better among the candidate solutions for the fixation of total ${\gamma}$-radioactivity. 134,137Cs which is composed of more than 85 % of total ${\gamma}$-radioactivity could be fixed effectively by the PVA-PMAA solution.

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The study for the preparations and analyses of fragrance oil encapsulation using $\beta$-cyclodextrin and arabic gum ($\beta$-cyclodextrin과 arabic gum를 이용한 향료의 encapsulation제법과 분석에 관한 연구)

  • 류영상;윤영수
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.21 no.1
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    • pp.84-94
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    • 1995
  • Fragrance oil was encapsulated with B-Capsulation and Arabic gum for double Encapsulation to improve the demerits-cause the skin irritation, allergy and lack of odor fixative-. Powder fragrance was obtained from the paste phase using the method of spray dry. From the analyses (GC, IR, UV) and sensory teste, it was identified that frogrance was encapsulated in our reservoir.

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Experimental study of file filling meterial with A thickener (증점제를 첨가한 매입말뚝 주면고정액의 실험적 연구)

  • Ko, Hye-Bin;Lee, Han-Seung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2020.06a
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    • pp.21-22
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    • 2020
  • In this study, the pile filling materials of the pile in drilled piling was studied. cement milk is mostly used as the filling materials of bored pile. The use of filling material based on cement milk is inefficient at field construction because it needs a lot of the charging mass. thickening agent was added to the cement milk to perform settlement estimation experiment on a circular cylinder, and as a result of examining the compressive strength of the day, it was found that the settlement estimation was significantly reduced. However, the strength was relatively low, it was confirmed that there was no problem with the regulation because the main surface fixative required relatively low strength.

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Arthrodesis of the Knee with Vascularized Fibular Graft - A Case of Infected Total Knee Arthroplasty - (혈관 부착 비골 전위술을 이용한 슬관절 유합술 - 슬관절 전치환술 후 감염이 합병된 증례 -)

  • Chung, Duke-Whan;Han, Chung-Soo;Lee, Jae-Hoon;Jeong, Sun-Teak;Park, Jin-Sung
    • Archives of Reconstructive Microsurgery
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    • v.15 no.2
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    • pp.111-116
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    • 2006
  • An infection after total knee arthroplasty has many complications such as severe bone defect, skin and soft tissue problems, devastated general condition, so arthrodesis is preferred as treatment option. However, poor bony contact due to severe bone defect and inadequate conditions of the soft tissue often cause nonunion or severe limb shortening after arthrodesis. More over these conditions, it is not easy to choose appropriate fixative devices. In these situations, the arthrodesis using vascularized fibular graft can be the solution. Vascularized fibular graft (VFG) can playa role as a suitable material for the treatment of bone defects. And VFG can overcome poor blood circulation caused by scar tissues, and can be relatively more durable and adequate length. In the long term, VFG can be hypertrophied by weight bearing, and will give mechanical stablility. The purpose of the paper is to report the successful results of arthrodesis using VFG in a patient who got extensive bone defect after failed revision total knee arthroplasty with infection.

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Morphological Study on the Mast Cell of Proventriculus in Pheasant (Phasianus colchicus) (꿩 전위의 비만세포에 관한 형태학적 연구)

  • Lee Y. H.
    • Korean Journal of Poultry Science
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    • v.32 no.2
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    • pp.97-100
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    • 2005
  • Mast cells have been studied extensively in various animals including rats and mice, whereas little is known the morphological data about pheasant mast cells. Here, morphological features of Korean pheasant mast cells are described in this study using light and electron microscopes. For light microscopy, mast cells had many metachromatic granules stained with toluidine blue in the cytoplasm. The fixation with $10\%$ neutral buffered formalin blocked staining of most mast cells but a modified Karnovsky solution proved to be a good fixative. In Korean pheasants, toluidine blue stained more mast cells than did alcian blue. For electron microscopy, the mast cells of the Korean pheasant were round, oval, spindle-like and irregular form and occasionally had a few short cytoplasmic processes. These cells had membrane-bounded granules and poorly developed organells. Some granules in the cytoplasm of the mast cells had bilayer membrane. Most granules were round shape and the membrane of several granules was concave or convex. The granules were composed of three parts, homogenous, particulate and reticular pattern.

Optimising the Extraction of Bacteria, Heterotrophic Protists and Diatoms, and Estimating Their Abundance and Biomass from Intertidal Sandy Sediments

  • Lee, Won-Je;Patterson, David J.
    • Journal of the korean society of oceanography
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    • v.37 no.2
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    • pp.58-65
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    • 2002
  • The study of microbial communities in sediments is impaired by the lack of reliable extraction methods. This study reports on the efficiency of recovery of a method for extracting bacteria, protists and diatoms from sandy sediments using a modified decant/fix method. The best extractions were achieved after fixation with a microtubule stabilising fixative and subsequent sonication for 80 seconds. We estimate that the efficiencies of recovery of bacteria and mixed heterotrophic flagellates were $96.4{\pm}3.5\%$ and $96.9{\pm}4.6\%$, respectively. Diatoms were recovered with an efficiency of 38-83% and varied considerably from species to species. This study suggests that the decant/fix method is effective in extracting small cells such as bacteria and heterotrophic flagellates, and that the efficiency of recovery of the method varies due to cell length and different types of organisms. When microbial carbon biomass had been underestimated by up to 32%, with much of that relating to larger cells such as microalgae and ciliates. We note that the corrected abundances may be still a subset of the total numbers present.

Single-cell PCR on protargol-impregnated euplotid ciliates: a combined approach of morphological and molecular taxonomy

  • Kim, Se-Joo;Choi, Joong-Ki;Ryu, Seong-Ho;Min, Gi-Sik
    • Animal cells and systems
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    • v.15 no.3
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    • pp.251-258
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    • 2011
  • Ciliates are considered one of the most diverse protozoa and play significant roles in ecology. For successful taxonomic study of these microscopic eukaryotes, a staining procedure is necessary, due mainly to intrinsic difficulties in recognizing characteristics from living cells. Although molecular taxonomy has been used to resolve the ambiguities associated with traditional morphology-based taxonomy, extraction of genomic DNA from stained ciliate cells is not available yet. In the present study, we describe a method to extract genomic DNA from a single protargol-impregnated euplotid cell. By using $HgCl_2$ as a fixative and modulating the exposure time of bleach solution in the protargol impregnation, high-quality genomic DNA can successfully be extracted from a stained single cell with minimal loss of morphological integrity. This technique will contribute to the effectiveness of combined approaches of molecular and morphological taxonomy from single ciliate cells.

A Case of Intoxication of Ingested Formalin (포르말린에 의한 급성 중독 1례)

  • Baek, Seon-Hee;Kim, Kyung-Hwan;Park, Jun-Seok;Shin, Dong-Wun;Roh, Jun-Young;Lee, Kyoung-Mi;Kim, Ah-Jin
    • Journal of The Korean Society of Clinical Toxicology
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    • v.7 no.1
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    • pp.38-40
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    • 2009
  • Formalin is a water-soluble, colorless, pungent, irritating and highly reactive gas. A 40% solution of formaldehyde in water, also known as formalin, is used as a disinfectant, antiseptic, deodorant, tissue fixative and embalming fluid. Ingestion can lead to immediate deleterious effects on almost all systems of the body including gastrointestinal tract, central nervous system, cardiovsacular system and hepato-renal system, causing gastrointestinal hemorrhage, cardiovsacular collapse, unconsciousness or convulsions, severe metabolic acidosis and acute respiratory distress syndrome. We treated a 39-year-old woman who ingested 300 ml formalin in a suicidal attempt. Despite hemodialysis, death occurred after 23 h.

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Study on Dying with Yellow Flowers - From Wild Flowers - (황화(黃花) 식물의 염색연구 - 들꽃을 중심으로 -)

  • Woo, Hyun-Ri;Kim, Sun-Mee
    • Journal of the Korea Fashion and Costume Design Association
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    • v.10 no.2
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    • pp.1-10
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    • 2008
  • Yellow, one of the five direction colors, is a prevailing color in artificial dyeing. The color has implied and symbolized wealth and power since ancient times. Yellow has been extracted mainly from flowers. Shapes, colors and scents of flowers not only have enriched emotional mentality, but have also been used for medicinal herbs, and dyestuff since the very old days. Coloring matter from flowers is very beautiful, and it has been used for dyeing textiles. Textile dyeing have formed a color culture and developed a dyeing culture since ancient times. Flowers include a variety of color matters, and can be easily obtained around us. Therefore, flowers have been a widespread natural dyestuff. It is well known that beautiful colors can be extracted from flowers, which are eco-friendly and non-polluting. In addition, flowers are easily provided. In this study, yellow wild flowers were selected as subjects. Seven flowers with color matters distiguishable through the naked eye were used: Korean forsythia, golden-wave, Mongolian dandelion, sunflower, conflower, chelidonium and chrysanthemum. Coloring matters were extracted from the seven flowers, which have been used to create a variety of colors through various dyestuffs; and the colors have been analyzed and presented. Dyeing and coloring matters were tested and analyzed, and several types of dyestuff had also been scientifically measured after treatment.

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Reconstruction of extended orbital floor fracture using an implantation method of gamma-shaped porous polyethylene

  • Hwang, Woosuk;Kim, Jin Woo
    • Archives of Craniofacial Surgery
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    • v.20 no.3
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    • pp.164-169
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    • 2019
  • Background: The conventional surgical method for reconstructing orbital floor fractures involves restoration of orbital continuity by covering an onlay with a thin material under the periorbital region. However, in large orbital floor fractures, the implant after inserting is often dislocated, leading to malposition. This study aimed to propose a novel implanting method and compare it with existing methods. Methods: Among patients who underwent surgery for large orbital floor fractures, 24 who underwent the conventional onlay implanting method were compared with 21 who underwent the novel ${\gamma}$ implanting method that two implant sheets were stacked and bent to resemble the shape of the Greek alphabet ${\gamma}$. When inserting a ${\gamma}$-shaped implant, the posterior ledge of the orbital floor was placed between the two sheets and the bottom sheet was impacted onto the posterior wall of the maxilla to play a fixative role while the top sheet was placed above the residual orbital floor to support orbital contents. Wilcoxon signed-rank test and Mann-Whitney U test were used for data analyses. Results: Compared to the conventional onlay method, the gamma method resulted in better restoration of orbital contents, better improvement of enophthalmos, and fewer revision surgeries. Conclusion: Achieving good surgical outcomes for extended orbital floor fractures is known to be difficult. However, better surgical outcomes could be obtained by using the novel implantation method of impacting a ${\gamma}$-shaped porous polyethylene posteriorly.