• Journal of Microbiology and Biotechnology
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• v.8 no.5
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• pp.517-522
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• 1998

The cry1 gene content of a collection of Bacillus thuringiensis strains, which include new isolates from Malaysia and Indonesia, was determined by a multiplex PCR using a set of eight oligonucleotide forward primers specific to cry1Aa, cry1Ab, cry1Ac, cry1Ba, cry1Ca, cry1Da, cry1Ea, and cry1Fa genes, and two reverse primers, one specific to cry1Ab and the other common to the remaining cry1 genes. Two-thirds of the 59 strains screened were cry1 positive and contained one to four different genes. The cry gene profiles correlated well with toxicities of the strains to lepidopteran insects. The method can be used for rapid screening of a large number of new isolates as the total DNA extracted by boiling cells from single colonies can be used directly in the PCR. However, it is not suitable for follow-up monitoring of specific commercial strains after application in the field as the PCR product profiles of these strains could not be differentiated from those of new isolates.

• Korean Journal of Veterinary Research
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• v.18 no.2
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• pp.61-67
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• 1978

The detection of Bordetella bronchiseptica which is supposed to be an agent of the infectious atrophic rhinitis of swine, is likely to receive more attention in the future as the pork industry comes to realize that eradication of this infection from breeding herds is a practical possibility. Experiments described here were carried out to establish the rapid plate agglutination test for the detection of the infectious atrophic rhinitis of swine in the field using the criteria of antigen preparation, effects on the antigenecity after storing of the antigen and reaction appearing time. Also, the agglutinabilities between the plate and tube method were compared and the degree of pathological lesions were recorded in relation to tube agglutination titers. Obtained results were as follows: 1. No differences were noted in the agglutinabilities on the plate agglutination test between the treatments in antigen preparation-formolized, merthiolate-killed and living organism. 2. The agglutinability of the antigens did not show any significant changes until 10 weeks of storage at 4 C; however, after 10 weeks of storage, non-specific reaction was observed with the HPCD control sera. 3. The results of the plate and tube agglutination tests were not comparable but the effective use of the plate method in Bordetella bronchiseptica eradication programs in pigs especially in the sow is stressed as a screening test.

• The Bulletin of The Korean Astronomical Society
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• v.40 no.1
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• pp.56.2-56.2
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• 2015

The KMTNet Supernovae Project utilizes the large $2^{\circ}{\times}2^{\circ}$ field of view of the three KMTNet telescopes to search and monitor supernovae, especially early ones, and other optical transients. A key component of the project is to build a data pipeline with a descent latency and an early alerting system that can handle the large volume of the data in an efficient and a prompt way, while minimizing false alarms, which casts a significant challenge to the software development. Here we present the current status of their development. The pipeline utilizes a difference image analysis technique to discover candidate transient sources after making correction of image distortion. In the early phase of the program, final selection of transient sources from candidates will mainly rely on multi-filter, multi-epoch and multi-site screening as well as human inspection, and an interactive web-based system is being developed for this purpose. Eventually, machine learning algorithms, based on the training set collected in the early phase, will be used to select true transient sources from candidates.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2012.05a
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• pp.252-255
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• 2012

Multimedia element of the game graphics and sound, etc. 'commitment' that plays an important role in gameplay. Therefore, the quality of the game is closely related to the quality of multimedia elements that can be. In this study, various competitions in the field of content screening criteria based on previous studies and multimedia elements of the game area, and domain-specific details about the quality of sub-elements were extracted. Assessment areas and sub-elements extracted to verify the need for the game will be carried out surveys among the users. The findings in the next game production shall be deemed to be an important reference material.

• Korean Journal of Childcare and Education
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• v.13 no.1
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• pp.215-232
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• 2017

Objective: The purpose of this study is to explore the ways in which childcare teachers understand and implicate the standards of national competency by searching for and analyzing the awareness of it. Methods: For this, the survey was conducted on 264 child care teachers working in daycare centers. The screening tool was constructed based on the content provided in the child care field of the NCS, and the questionnaire items were developed by referring to previous research. Results: Recognition of the child care teacher's awareness of the NCS was very low. The activity of child care teachers for the category of capability proposed by the NCS showed significant differences depending on work experience. The degree of awareness of child care teachers for the category of capability proposed by the NCS showed significant differences depending on work experience. The difference between the activity of child care teachers and the degree of awareness of child care teachers for the category of capability proposed by the NCS showed significant differences. Conclusion: In the future, it is necessary for the NCS to be constantly supplemented and ensure that the capability to perform practical functions is fulfilled in daycare centers.

• Genomics & Informatics
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• v.14 no.4
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• pp.160-165
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• 2016

Over the past decade, the detection of gene-gene interactions has become more and more popular in the field of genome-wide association studies (GWASs). The goal of the GWAS is to identify genetic susceptibility to complex diseases by assaying and analyzing hundreds of thousands of single-nucleotide polymorphisms. However, such tests are computationally demanding and methodologically challenging. Recently, a simple but powerful method, named "BOolean Operation-based Screening and Testing" (BOOST), was proposed for genome-wide gene-gene interaction analyses. BOOST was designed with a Boolean representation of genotype data and is approximately equivalent to the log-linear model. It is extremely fast, and genome-wide gene-gene interaction analyses can be completed within a few hours. However, BOOST can not adjust for covariate effects, and its type-1 error control is not correct. Thus, we considered two-step approaches for gene-gene interaction analyses. First, we selected gene-gene interactions with BOOST and applied logistic regression with covariate adjustments to select gene-gene interactions. We applied the two-step approach to type 2 diabetes (T2D) in the Korea Association Resource (KARE) cohort and identified some promising pairs of single-nucleotide polymorphisms associated with T2D.

• Journal of the Korean Society of Tobacco Science
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• v.26 no.2 s.52
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• pp.79-84
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• 2004

During the screening of antiviral substances having inhibitory effects on tobacco mosaic virus (TMV) infection to tobacco plants, we found a bacterial isolate KTGBP10, which was identified as a Stenotrophomonas sp., strongly inhibited the infection of TMV. When the culture filtrate from KTGBP10 was applied on the upper surface of leaves of Xanthi-nc tobacco plants at the same time or 24 hours before TMV inoculation, almost complete inhibition of TMV infection was achieved. And $40\%$ inhibition was shown with application of the culture filtrate to the under surface of leaves. In field trials, transmission of TMV from diseased seedlings to the healthy ones during transplanting work was reduced by $87.1\~92.6\%$ when the culture filtrate or cell suspension was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. When the broth filtrate of KTGBP10 was supplied by soaking through the cut-leaves before and/or after virus inoculation, the TMV infection was also inhibited by $50.4\~65.3\%$.

• Korean Management Science Review
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• v.32 no.1
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• pp.35-47
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• 2015

The purpose of this paper is to find an optimal weaving connection method of sweater threads while weaving intarsia sweater by the genetic algorithm. The objective function was devised to minimize labor cost and lessen the amount of thread usage. In order to create the parental population group in the genetic algorithm, we developed five thread connection methods. Besides, elite chromosome screening methods for the offspring group was selected both to the whole chromosome thread elite and to a color-coded elite thread chromosome. Commonly used diamond pattern in Intarsia sweater manufacturing was applied to the experiments. The experimental results showed that thread system saved the labor and material costs than woven method under the existing software. When weaving Intarsia sweater in the field, we can apply the developed genetic algorithm to improve productivity of weaving connection method.

• Korea-Australia Rheology Journal
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• v.16 no.1
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• pp.17-26
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• 2004

We examine rigorous computations on microstructural as well as rheological properties of concentrated dispersions of bidisperse colloids. The NVT Monte Carlo simulation is applied to obtain the radial distribution function for the concentrated system. The long-range electrostatic interactions between dissimilar spherical colloids are determined using the singularity method, which provides explicit solutions to the linearized electrostatic field. The increasing trend of osmotic pressure with increasing total particle concentration is reduced as the concentration ratio between large and small particles is increased. From the estimation of total structure factor, we observe the strong correlations developed between dissimilar spheres. As the particle concentration increases at a given ionic strength, the magnitude of the first peak in structure factors increases and also moves to higher wave number values. The increase of electrostatic interaction between same charged particles caused by the Debye screening effect provides an increase in both the osmotic pressure and the shear modulus. The higher volume fraction ratio providing larger interparticle spacing yields decreasing high frequency limit of the shear modulus, due to decreasing the particle interaction energy.

• The Plant Pathology Journal
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• v.20 no.4
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• pp.293-296
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• 2004

During the screening of antiviral substances having inhibitory effects on Tobacco mosaic virus (TMV) infection on tobacco plants, we found a bacterial isolate KTB3, and identified it as Acinetobacter sp. which strongly inhibited the infection of TMV When the culture filtrate from KTB3 was applied on the upper surface of the Xanthi-nc tobacco leaves at the same time, or 24 hours before TMV inoculation, almost complete inhibition was achieved. Likewise, 86% inhibition was achieved, when the culture filtrate was applied on the underside of the leaves. In field trials, transmission of TMV from diseased seedlings to healthy ones during transplanting work was reduced by 92%, when the culture filtrate was sprayed onto the tobacco seedlings, cv. NC82, 24 hours before transplanting. No toxic effect was observed on the tobacco plants. Antiviral substance from the culture filtrate was purified by ethanol precipitation, dialysis, DEAE-cellulose, and Sephadex G75 gel column chromatography. The partially purified active material which showed positive color reaction to sugar and protein inhibited TMV infection by 60% at 1 ${\mu}$g/ml.