• Preventive Nutrition and Food Science
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• v.22 no.2
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• pp.124-130
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• 2017

This paper describes in detail proximate composition, nutritional profile, phytochemical constituents, antioxidant activities, antimicrobial potential, and antihemolytic activity (towards human erythrocytes) of various fractions of wild Ganoderma lucidum. Proximate analysis established that wild G. lucidum comprises about $87.02{\pm}5.45%$ of moisture, and the remaining part is a rich source of proteins ($8.59{\pm}0.37%$), crude fiber ($54.21{\pm}1.2%$), and carbohydrate (35.16%) with smaller fat content (3.33 %). Similarly, phytochemical screening revealed the presence of flavonoids ($217.51{\pm}0.30mg/g$), ascorbic acid ($116{\pm}7.32mg/g$), phenolics ($360.72{\pm}34.07mg/g$), ${\beta}$-carotenes ($0.42{\pm}0.04{\mu}g/g$), and lycopene ($0.05{\pm}0.00{\mu}g/g$). Extracts of wild G. lucidum in various solvents provided first line protection against Escherichia coli and Pasteurella multocida in the order of ethyl acetate> ethanol> methanol> n-hexane> water. Furthermore, aqueous and methanolic extracts of wild G. lucidum were found to be safe towards human erythrocytes. Overall, wild mushroom (G. lucidum) was found to be a good source of dietary supplements, antimicrobial and antioxidant agents in the pursuance of its commercial utilization in food and pharmaceutical industries.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.9
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• pp.1431-1441
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• 2018

Objective: This study was conducted to assess the effects of replacing rice straw with different proportions of cassava pulp on growth performance, feed intake, digestibility, rumen microbial population, energy partitioning and efficiency of metabolizable energy utilization in beef cattle. Methods: Eighteen yearling Thai native beef cattle (Bos indicus) with an average initial body weight (BW) of $98.3{\pm}12.8kg$ were allocated to one of three dietary treatments and fed ad libitum for 149 days in a randomized complete block design. Three dietary treatments using different proportions of cassava pulp (100, 300, and 500 g/kg dry matter basis) instead of rice straw as a base in a fermented total mixed ration were applied. Animals were placed in a metabolic pen equipped with a ventilated head box respiration system to determine total digestibility and energy balance. Results: The average daily weight gain, digestible intake and apparent digestibility of dry matter, organic matter and non-fiber carbohydrate, total protozoa, energy intake, energy retention and energy efficiency increased linearly (p<0.05) with an increasing proportion of cassava pulp in the diet, whereas the three main types of fibrolytic bacteria and energy excretion in the urine (p<0.05) decreased. The metabolizable energy requirement for the maintenance of yearling Thai native cattle, determined by a linear regression analysis, was $399kJ/kg\;BW^{0.75}$, with an efficiency of metabolizable energy utilization for growth of 0.86. Conclusion: Our results demonstrated that increasing the proportion of cassava pulp up to 500 g/kg of dry matter as a base in a fermented total mixed ration is an effective strategy for improving productivity in zebu cattle.

• Composites Research
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• v.13 no.6
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• pp.39-46
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• 2000

Electrical discharge machining (EDM) cuts metal by discharging electric current across a thin gap between tool and workpiece. Electrical discharge wire cutting, a special form of EDM, uses a continuously moving conductive wire as an electrode, and is widely used for the manufacture of punches, dies and stripper plates. In the wire cutting process, the moving wire is usually supported by cantilever arm and wire guides. As the wire traveling speed has been increased in recent years to improve productivity, the vibration of the cantilever arm occurs, which reduces the positional accuracy of the machine. Therefore, the design and manufacture of the cantilever arm with high dynamic characteristics have become important as the machining speed increases. In this paper, the cantilever arm for guiding the moving wire was designed and manufactured using carbon fiber epoxy composite in order to improve the static and dynamic characteristics. Specimens for the composite cantilever arm were manufactured and tested to investigate the effect of the number of reinforcing plies and length fitted to steel flange on the load capacity. Also, the finite element analysis using layer and contact elements was performed to compare the calculated results with the experimental ones. From the results, the prototype of the composite cantilever arm for the electrical discharge wire cutting machine was manufactured and the static and dynamic characteristics were compared with those of the conventional steel cantilever arm.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.36 no.8
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• pp.889-895
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• 2012

Carbon fiber reinforced plastic (CFRP) composites have high specific stiffness and high specific strength. Therefore, they are increasingly being use, instead of conventional metallic materials in the aviation and automobile industries, where there is a strong demand for lightweight materials. In aircraft, the fuselage is exposed to severe conditions of high temperatures and high humidity. Therefore, it is necessary to estimate the strength of CFRP composites under real conditions from the viewpoint of aircraft safety. In this study, CFRP specimens were immersed in distilled water at $75^{\circ}C$ for a long time. Then, tensile tests were performed on these specimens, and the fracture characteristics of the fractured surfaces were analyzed using SEM. A fatigue test was performed on specimens immersed for 300 days with R=0.1, and it was confirmed that the fatigue life deteriorated in immersed specimens compared to specimens that were not immersed.

• Advances in concrete construction
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• v.8 no.4
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• pp.257-267
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• 2019

This paper presents the results of cyclic loading tests on new high-strength concrete (HC) short columns. The seismic performance and deformation capacity of three reinforced high-strength concrete filled Polyvinyl Chloride tube (RHC-PVCT) short columns and one reinforced high-strength concrete (RHC), under pseudo-static tests (PSTs) with vertical axial force was evaluated. The main design parameters of the columns in the tests were the axial compression ratio, confinement type, concrete strength, height-diameter ratio of PVCT. The failure modes, hysteretic curves, skeleton curves of short columns were presented and analyzed. Placing PVCT in the RHC column could be remarkably improved the ultimate strength and energy dissipation of columns. However, no fiber element models have been formulated for computing the seismic responses of RHC-PVCT columns with PVT tubes filled with high-strength concrete. Nonlinear finite element method (FEM) was conducted to predict seismic behaviors. Finite element models were verified through a comparison of FEM results with experimental results. A parametric study was then performed using validated FEM models to investigate the effect of several parameters on the mechanical properties of RHC-PVCT short columns. The parameters study indicated that the concrete strength and the ratio of diameter to height affected the seismic performance of RHC-PVCT short column significantly.

• Advances in concrete construction
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• v.8 no.4
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• pp.335-349
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• 2019

To investigate the axial compressive performance of the recycled aggregate concrete (RAC) filled glass fiber reinforced polymer (GFRP) tube and profile steel composite columns, static loading tests were carried out on 18 specimens under axial loads in this study, including 7 RAC filled GFRP tube columns and 11 RAC filled GFRP tube-profile steel composite columns. The design parameters include recycled coarse aggregate (RCA) replacement percentage, profile steel ratio, slenderness ratio and RAC strength. The failure process, failure modes, axial stress-strain curves, strain development and axial bearing capacity of all specimens were mainly analyzed in detail. The experimental results show that the GFRP tube had strong restraint ability to RAC material and the profile steel could improve the axial compressive performance of the columns. The failure modes of the columns can be summarized as follow: the profile steel in the composite columns yielded first, then the internal RAC material was crushed, and finally the fiberglass of the external GFRP tube was seriously torn, resulting in the final failure of columns. The axial bearing capacity of the columns decreased with the increase of RCA replacement percentage and the maximum decreasing amplitude was 11.10%. In addition, the slenderness ratio had an adverse effect on the axial bearing capacity of the columns. However, the strength of the RAC material could effectively improve the axial bearing capacity of the columns, but their deformability decreased. In addition, the increasing profile steel ratio contributed to the axial compressive capacity of the composite columns. Based on the above analysis, a formula for calculating the bearing capacity of composite columns under axial compression load is proposed, and the adverse effects of slenderness ratio and RCA replacement percentage are considered.

• Structural Engineering and Mechanics
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• v.72 no.6
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• pp.713-722
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• 2019

Micromechanics is a technique for the analysis of composites or heterogeneous materials which focuses on the components of the intended structure. Each one of the components can exhibit isotropic behavior, but the microstructure characteristics of the heterogeneous material result in the anisotropic behavior of the structure. In this research, the general mechanical properties of a 3D anisotropic and heterogeneous Representative Volume Element (RVE), have been determined by applying periodic boundary conditions (PBCs), using the Asymptotic Homogenization Theory (AHT) and strain energy. In order to use the homogenization theory and apply the periodic boundary conditions, the ABAQUS scripting interface (ASI) has been used along with the Python programming language. The results have been compared with those of the Homogeneous Boundary Conditions method, which leads to an overestimation of the effective mechanical properties. According to the results, applying homogenous boundary conditions results in a 33% and 13% increase in the shear moduli G₂₃ and G₁₂, respectively. In polymeric composites, the fibers have linear and brittle behavior, while the resin exhibits a non-linear behavior. Therefore, the nonlinear effects of resin on the mechanical properties of the composite material is studied using a user-defined subroutine in Fortran (USDFLD). The non-linear shear stress-strain behavior of unidirectional composite laminates has been obtained. Results indicate that at arbitrary constant stress as 80 MPa in-plane shear modulus, G₁₂, experienced a 47%, 41% and 31% reduction at the fiber volume fraction of 30%, 50% and 70%, compared to the linear assumption. The results of this study are in good agreement with the analytical and experimental results available in the literature.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.4
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• pp.457-463
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• 2016

The goal of this work was to investigate the correlations between MyHC mRNA transcription and their corresponding protein expressions in porcine longissimus muscle (LM) during postnatal growth of pigs. Five DLY ($Duroc{\times}Landrace{\times}Yorkshire$) crossbred pigs were selected, slaughtered and sampled at postnatal 7, 30, 60, 120, and 180 days, respectively. Each muscle was subjected to quantity MyHCs protein contents through an indirect enzyme-linked immunosorbent assay (ELISA), to quantity myosin heavy-chains (MyHCs) mRNA abundances using real-time polymerase chain reaction. We calculated the proportion (%) of each MyHC to total of four MyHC for two levels, respectively. Moreover, the activities of several key energy metabolism enzymes were determined in LM. The result showed that mRNA transcription and protein expression of MyHC I, IIa, IIx and IIb in LM all presented some obvious changes with postnatal aging of pigs, especially at the early stage after birth, and their mRNA transcriptions were easy to be influenced than their protein expressions. The relative proportion of each MyHC mRNA was significantly positively related to that of its corresponding protein (p<0.01), and MyHC I mRNA proportion was positively correlated with creatine kinase (CK), succinate dehydrogenase (SDH), malate dehydrogenase (MDH) activities (p<0.05). These data suggested that MyHC mRNA transcription can be used to reflect MyHC expression, metabolism property and adaptive plasticity of porcine skeletal muscles, and MyHC mRNA composition could be a molecular index reflecting muscle fiber type characteristics.

• Journal of Korean Society for Atmospheric Environment
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• v.7 no.1
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• pp.1-16
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• 1991

In this study, sampling and analytical procedures were evaluated for the determination of ambient levels of atmospheric PAH, both in gaseous and particulate phase. The method involves low-volume sampling and Soxhlet extraction of the filters and Tenax absorbent, followed by a clean-up stage using a silica column prior to analysis by reversed-phase HPLC with wavelength programmable fluorescence and UV detection. A total of 18 PAH were identified and quantified, all of which have been of environmental concern. In order to validate the methodology and to ensure compatibility of the results, the analytical method used for the determination of PAH was evaluated with respect to the efficiencies of extraction and clean-up procedure, HPLC separation, and lower limits of detection. In addition, substrate dependency of PAH recovery was investigated for the two types of fiters, i.e. glass fiber and PTFE filters.

• Nutrition Research and Practice
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• v.8 no.4
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• pp.398-403
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• 2014

BACKGROUND/OBJECTIVES: Ultraviolet B (UVB) irradiation on skin can induce production of reactive oxygen species (ROS), which cause expression of matrix metalloproteinases (MMPs) and collagen degradation. Thus, chronic exposure of skin to UVB irradiation leads to histological changes consistent with aging, such as wrinkling, abnormal pigmentation, and loss of elasticity. We investigated the protective effect of the standardized green tea seed extract (GSE) on UVB-induced skin photoaging in hairless mice. MATERIALS/METHODS: Skin photoaging was induced by UVB irradiation on the back of Skh-1 hairless mice three times per week and UVB irradiation was performed for 10 weeks. Mice were divided into six groups; normal control, UVB irradiated control group, positive control (UVB + dietary supplement of vitamin C 100 mg/kg), GSE 10 mg/kg (UVB + dietary supplement of GSE 10 mg/kg), GSE 100 mg/kg (UVB + dietary supplement of GSE 100 mg/kg), and GSE 200 mg/kg (UVB + dietary supplement of GSE 200 mg/kg). RESULTS: The dietary supplement GSE attenuated UVB irradiation-induced wrinkle formation and the decrease in density of dermal collagen fiber. In addition, results of the antioxidant analysis showed that GSE induced a significant increase in antioxidant enzyme activity compared with the UVB irradiation control group. Dietary supplementation with GSE 200 mg/kg resulted in a significant decrease in expression of MMP-1, MMP-3, and MMP-9 and an increase in expression of TIMP and type-1 collagen. CONCLUSIONS: Findings of this study suggest that dietary supplement GSE could be useful in attenuation of UVB irradiation-induced skin photoaging and wrinkle formation due to regulation of antioxidant defense systems and MMPs expression.