• Title/Summary/Keyword: extraction solvents

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Biological Activities and Metabolite Analysis of Various Extracts and Fractions from Red Ginseng Marc

  • Lee, Dong Gyu;Jang, Ik Soon;Kang, Young-Hwa
    • Korean Journal of Plant Resources
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    • v.33 no.6
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    • pp.597-603
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    • 2020
  • Red ginseng marc (RGM) has been used on primary industries using fertilizer or forage, and it mostly has been dumped. To improve utilization of RGM, the biological activities of RGM were examined. RGM was extracted and fractionated using various solvents and their biological activities were compared. The hexane fraction from the methanol extract of RGM (RGMMH) showed strong anti-cancer activity (58.56 ± 6.04% at 100 ㎍/mL) and anti-inflammatory effect (65.72 ± 1.33% at 100 ㎍/mL). But, oil extract of RGM extracted with hexane (RGMH) showed low activities (anti-cancer: 16.42 ± 3.33%, at 100 ㎍/mL, anti-inflammatory activity: 29.46 ± 2.10%, at 100 ㎍/mL). Their metabolites were analyzed using HPLC. Panaxydol known as anti-cancer compound of RGM was one of major compounds in RGMMH. Meanwhile, panaxydol was detected in trace amount in red ginseng marc oil (RGMH). In addition, RGMMH and RGMH showed big differences in HPLC profiling. This research suggests optimal extraction method of RGM oil.

Antioxidant Properties of Unripened Apple Extracts (미숙사과 용매 추출물의 항산화성)

  • Won Hyang Rye;Park Mee Weon;Choi Moo Young
    • The Korean Journal of Community Living Science
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    • v.16 no.2
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    • pp.11-16
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    • 2005
  • The purpose of this study was to investigate the antioxidant properties of unripened apple extracts. The amount of total flavonoids in the peel and 5/30 samples were 2.7times and 5.0 times higher than the flesh and 6/30 samples, respectively. The degree of angintensin I converting enzyme (ACE) inhibition activity in hot water extracts was higher than the other extracts. In the 5/30 sample, a $0.1\%$ concentration of unripened apple powder showed almost the same electron-donating ability with a $0.5\%$ concentration of the 6/30 sample. More than $90\%$ of the electron-donating ability was observed from the peel extracts regardless of solvents used for extraction. The nitrite-scavenging effects of acetone and methanol extracts of the 5/30 sample were the strongest at pH 1.2, and especially the $80\%$ methanol extracts exhibited a powerful scavenging effect of more than $90\%$ at pH 3.0.

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Quantitative Analysis of Relative Adsorption of Disperse Dyes on Polyurethane in Polyurethane-impregnated Polyester Microfibers (폴리우레탄함침 폴리에스터 극세사 소재의 분산염료 염색시 폴리우레탄 성분에 대한 염료의 상대염착률 정량분석)

  • Jun, Heejeong;Park, Suhyeon;Lee, Junheon;Kim, Taekyeong
    • Textile Coloration and Finishing
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    • v.32 no.3
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    • pp.121-127
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    • 2020
  • The analysis method of relative adsorption of disperse dyes on impregnated polyurethane resin and polyester fiber separately was investigated. The solvents for extracting the dyes from polyurethane resin and polyester fiber were determined by acetone and DMF, respectively. By extracting the dyed fibers with acetone at room temperature within 30 minutes, the dyes adsorbed only on the polyurethane resin could be extracted. And then by additional extracting the same fabrics with DMF at 95℃ for longer than 1 hour, the dyes adsorbed on polyester fabrics was extracted. This means that the dyes adsorbed on polyurethane and polyester could be extracted separately and that relative adsorption of the dyes on the both components could be analyzed quantitatively. Using this analysis method, the relative adsorption of a disperse dye was investigated after reduction clearing with various conditions.

Antifungal Activities on Organic Heritage Fungi and Antioxidative effect of Phellodendron amurense Extractives (황벽나무 추출물의 유기질 문화재 오염균에 대한 항균성 및 항산화 활성)

  • Hong, Jin-Young;Kim, Young-Hee;Jung, Mi-Hwa;Jo, Chang-Wook;Choi, Jung-Eun
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.42 no.5
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    • pp.54-61
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    • 2010
  • Antifungal and antioxidative activities of Phellodendron amurense extracts were investigated for use as a natural preservative. After separation of P. amurense into phloem, xylem, leaf and fruit each part was subjected to methanol extraction. Each MeOH extract was further fractionated with several solvents(n-hexane, methylene chloride and ethylacetate). Among the methanol extracts, extracts of phloem and leaf inhibited effectively the growth of mold fungi and rot fungi, respectively. Especially, ethylacetate fraction from phloem showed the highest growth inhibitory effects against fungi tested, such as P. citreonigrum H3, P. toxicarium H4, P. corylophilu H5, A. clavatus, P. osteatus, S. commune, and G. lucidum. The fractions of fruit, which had lower antifungal activities mostly than those of phloem, strongly inhibited rot fungi such as G. lucidum, T. versicolor, and T. palustris. Compared to ferulic acid which is well known antioxidant, ethylacetate fraction of fruit showed high antioxidative activities on concentration of 1 to $100{\mu}g/m{\ell}$ in DPPH radical scavenging activity. As a result, antifungal and antioxidative activities of P. amurense suggest that its extract and fraction have a possibility as conservative of cultural heritage because it might get conservation effects against deteriorating microorganisms of cultural heritage.

Investigation on Allelopathic potential of Korean Lycoris radiata (한국산 꽃무릇의 Allelopathy 효과 구명)

  • Park, Jae-Hyun;Lee, In-Jung;Shin, Dong-Hyun;Kim, Kil-Ung
    • Current Research on Agriculture and Life Sciences
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    • v.20
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    • pp.25-31
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    • 2002
  • This study was conducted to investigate allelopathic potential from Korean Lycoris radiata HERB. Two different solvents such as MeOH and water were used for extraction from Lycoris radiata HERB. Both extract showed inhibitory effect on the germination of barnyardgrass and lettuce seeds, indicating that this species contained the biologically active substances. The inhibitory substance extracted from bulbs of this species was identified by GC-MS as lycorenan, a kind of alkaloid.

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Synthesis and characterization of a new energy material (guanidinium dinitramide) with crystallization solvent

  • Kim, Wooram;Park, Mijung;Park, Yeonsoo;Kwon, Younja;Jo, Youngmin
    • Journal of Industrial and Engineering Chemistry
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    • v.68
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    • pp.153-160
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    • 2018
  • An environmentally favorable (chlorine-free) solid oxidizer, guanidinium dinitramide [GDN; $NH_2C(NH_2)NH_2N(NO_2)_2$], was newly synthesized from guanidine carbonate [$NH_2C(=NH)NH_2{\cdot}1/2H_2CO_3$]. Two different crystalline forms (${\alpha}-type$ and ${\beta}-type$) appeared according to the applied solvents and synthesis conditions. Moisture, during extraction, might become trapped in a crystal between inner molecules. Therefore, despite having the same chemical composition, Raman-IR and TGA-DSC revealed different physical characteristics of the two forms. Peaks of Raman shift near $1000cm^{-1}$ implied different chemical structures. Thermal analysis revealed an exothermic temperature $155.7^{\circ}C$ for ${\alpha}-type$ but one of $191.6^{\circ}C$ for ${\beta}-type$. The caloric value of ${\alpha}-type$ was 536.4 J/g, which was 2.5 times larger than that of the ${\beta}-type$, which was 1310 J/g. While the synthesized GDN of ${\alpha}-type$ showed a steep exothermic decomposition, the ${\beta}-type$ was slowly decomposed after melting through an endothermic process. This work implied that despite of the same molecular formula some different core thermal properties would appear depending on synthesis conditions.

Studies on Solvent Extraction and Flotation Technique Using Metal-Dithizone Complexes(II). Determination of Trace Elements in Water Samples by Solvent Sublation

  • 김영상;최윤석;최희선
    • Bulletin of the Korean Chemical Society
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    • v.19 no.10
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    • pp.1036-1042
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    • 1998
  • The preconcentration and determination of trace elements in water samples were studied by a solvent sublation utilizing dithizonate complexation. After metal dithizonates were formed, trace amounts of cadmium, cobalt, copper and lead were floated and extracted into small volume of a water-immiscible organic solvent on the surface of sample solution and determined in the solvent directly by GF-AAS. Several experimental conditions as formation condition of metal-dithizonate complexes, pH of solution, amount of dithizone, stirring time, the type and amount of surfactants, N2 bubbling rate and so on were optimized for the complete formation and effective flotation of the complexes. And also four kinds of light solvents were compared each other to extract the floated complexes, effectively. After the pH was adjusted to 4.0 with 5 M HNO3, 8.0 mL of 0.05% acetone solution of dithizone was added to 1.00 L water sample. The dithizonate complexes were flotated and extracted into the upper methyl isobutylketone (MIBK) layer by the addition of 2.0 mL 0.2% ethanolic sodium lauryl sulfate solution and with the aid of small nitrogen gas bubbles. And this solvent sublation method was applied to the analysis of real water samples and good results of more than 85% recoveries were obtained in spiked samples.

Assessment of The Level of Caffeine in Some Tea Leaves Marketed in Dutse: Jigawa State

  • BDULLAHI, R.;LAWAL, A.M.;IBRAHIM, M.S;KHALID, A.;MUHAMMAD, U.L.
    • The Korean Journal of Food & Health Convergence
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    • v.5 no.3
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    • pp.7-20
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    • 2019
  • The use of caffeine as a psychoactive stimulant in tea has been observed to have serious negative effects in humans' systems such as respiratory, nervous, cardiovascular, renal and skeletal systems. This study was carried out to assess the levels of caffeine in 10 different tea brands available in local market in Dutse, Jigawa State, Nigeria. Quantitative analysis of caffeine was performed by a simple and fast UV-Vis spectrophotometric methods using different solvents for extraction. The caffeine content in all the tea samples analyzed in this study were below the maximum allowable limits set by the USFDA. Tea have been associated with adverse health effects and the claims made by manufacturers about the benefits of tea do not highlight risks associated with excessive consumption of a combination of the ingredients contained in tea. Long term effects of tea consumption of children and young people have not been adequately studied. Therefore, it is recommended that further research be carried out on the adverse effects of energy drinks on children. Research is also needed to be done on the effects of the combination of ingredients on health and excessive consumption of those ingredients to children and adolescents. People need to be educated and given proper awareness on the health risks associated with caffeine containing beverages.

Antioxidant Component and Activity of Different Part Extracts in Apple (Malus domestica cv. Fuji) (사과 부위별 항산화 성분 및 항산화 활성)

  • Lee, Kyung-Haeng;Yoon, Ye-ji;Kwon, Hye-Won;Lee, Eun-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.31 no.6
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    • pp.858-864
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    • 2018
  • To improve the utility of apple, apple flesh (Malus domestica cv. Fuji), pomace and peels were dried and then extracted using solvents (water, 70% and 100% ethanol). The contents of polyphenols, flavonoids, ascorbic acid, ursolic acid and antioxidant activities were measured as extracted from the apple flesh, pomace and peel. The content of polyphenols was the highest in the apple peel when 100% ethanol was extracted. The content of flavonoids was about 3~8 times higher in apple peel than those of flesh and pomace, and 70% ethanol extract was the highest content. Ascorbic acid content was the highest in apple peel as well as flavonoid content. The content of ursolic acid in the apple flesh was not detected in water and ethanol extraction. In the case of apple pomace and peel, ursolic acid was not detected in water extract but it was detected when ethanol was extracted. The content of ursolic acid was highest in the apple peel. In the case of antioxidative activity, DPPH and ABTS radical scavenging ability showed the highest ability when apple peel was extracted with 70% ethanol.

Antibacterial activity of Dioscorea batatas, Morus alba, and Tagetes erecta against E. coli and S. aureus

  • Hyun-Seo Yoon;Chung Mu Park
    • Biomedical Science Letters
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    • v.29 no.3
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    • pp.130-136
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    • 2023
  • In this study, the anti-bacterial activity of three medicinal herbs such as Dioscorea batatas, Morus alba, and Tagetes erecta, have been applied in oriental medicine against Escherichia coli and Staphylococcus aureus. The antibacterial activity of Dioscorea batatas ethanol extract (DBEE), Morus alba ethanol extract (MAEE), and Tagetes erecta ethanol extract (TEEE) was analyzed by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. As a result, three medicinal herbs exhibited antibacterial activity in a dose-dependent manner as well as MAEE and TEEE showed the most potent antibacterial activity followed by disk diffusion assay against E. coli and S. aureus, respectively. MIC against E. coli and S. aureus was 1.6 mg/mL of MAEE and TEEE as well as 0.2 mg/mL of TEEE. MBC against both strains was 5 mg/mL of DBEE and 2.5 mg/mL of TEEE. Consequently, three medicinal herbs in this study showed potent antibacterial activity through the inhibited growth of E. coli and S. aureus, which is considered as a potential candidate for cosmeceutic agents to attenuate the pruritus. In addition, antibacterial and antihistamine effects followed by the extraction solvents should be analyzed in future studies.