• Title/Summary/Keyword: extract condition

Search Result 1,382, Processing Time 0.036 seconds

The Analysis of Vegetation-Environment Relationships of Mt. Jungwangsan by TWINSPAN(Two-Way Indicator Species Analysis) and DCCA(Detrended Canonical Correspondence Analysis) Ordination (TWINSPAN과 DCCA에 의한 중왕산(中旺山)의 삼림군집(森林群集)과 환경(環境)의 상관관계(相關關係) 분석(分析))

  • Song, Ho Kyung;Kwon, Ki Won;Lee, Don Koo;Jang, Kyu Kwan;Woo, In Shik
    • Journal of Korean Society of Forest Science
    • /
    • v.81 no.3
    • /
    • pp.247-254
    • /
    • 1992
  • Vegetational data obtained from 50 quadrats of Mt. Jungwangsan area were analysed by applying two multivariate methods : two-way indicator species analysis(TWINSPAN) for classification and detrended canonical correspondence analysis(DCCA) for ordination. DCCA technique allowed to extract the ordination axes that could be related to 15 environmental factors. The forest vegetation in Mt. Jungwangsan was classified into Quercus mongolica-Tilia amurensis, Quercus mongolica-Rhododendron schlippenbachii, Quencus mongolica-Kalopanax pictus, Quercus mongolica-Carpinus cordata, Quercus mongolica-Cornus controversa, Betula costata, Fraxinus mandshurica, and Ulmus laciniata communities according to the TWINSPAN. The relationships between the distribution of dominant species of forest vegetation and soil condition in Mt. Jungwangsan were investigated by analyzing elevation and soil nutrition gradient. Ulmus laciniata, Betula costata, and Fraxinus mandshurica forest were distributed in a ravine of the low elevation and in the good nutrition area of $Mg^{{+}{+}}$, total nitrogen, and $Ca^{{+}{+}}$, Quercus mongolica groups in the high elevation and in the poor nutrition area. Quercus mongolica-Kalopanax pictus forest of Quercus mongolica groups was distributed in the high elevation and in the good nutrition area of $Ca^{{+}{+}}$, C.E.C., $Mg^{{+}{+}}$, and total nitrogen, Quercus mongolica-Rhododendron schlippenbachii forest in the low elevation and poor nutrition area. Quercus mongolica-Carpinus cordata forest and Quercus mongolica-Cornus controversa forest were distributed in the medium elevation and medium nutrition area. The two dominant factors influencing community distribution were elevation and topography.

  • PDF

Changes in the Physicochemical and Antioxidant Characteristics during the Fermentation of Jujube Wine Using Hot Water Extract of Dried Jujube (건대추 열수추출물을 이용한 대추와인 발효중의 이화학 및 항산화적 특성 변화)

  • Eom, In-Ju;Choi, Jung-In;Kim, In-Ho;Kim, Tae-Hoon;Kim, Seong-Ho
    • Journal of Life Science
    • /
    • v.26 no.11
    • /
    • pp.1298-1307
    • /
    • 2016
  • In the study, we investigated the optimum fermentation conditions as well as changes of physicochemical and antioxidant characteristics during the fermentation of jujube wine. The physicochemical characteristics of the jujube hot water extracts used in this study were a pH of 5.05, 0.01% acidity, and $6.5^{\circ}Brix$ concentration. For jujube wine fermentation, the optimal fermentation strain was selected among the isolated strains and the final chosen strain was identified as Saccharomyces cerevisiae, based on the 26S rRNA gene sequencing and similarity searching in GenBank DB. The jujube wine fermented with an initial $15^{\circ}Brix$ concentration of jujube extracts showed a maximum alcohol content of 13% and lower residual sugar concentration. Alcohol content during the jujube wine fermentation was increased after 3 days of fermentation, and no significantly difference after 6 days was found. The residual sugar concentration during the fermentation periods was significantly decreased with increasing alcohol content. The jujube wine properties at 12 days of fermentation were as follows: a pH of 4.34, acidity of 0.29%, alcohol content of 12.8%, and a residual sugar concentration of $8.70^{\circ}Brix$. The malic acid content in the organic acid of fermented jujube wine was significantly decreased during the fermentation proceeding, whereas the succinic acid and lactic acid contents were significantly increased. Antioxidant characteristics of the fermented jujube wine were appeared ABTS radical scavenging activity 45.80%, DPPH radical scavenging activity 61.89%, nitrite scavenging activity 91.95% and total polyphenol compound 3.69 mg/ml. In terms of consumer liking of the jujube wine by sensory evaluation, the color and overall acceptability of jujube wine were evaluated as more than average.

Identification of Growth Inhibitory Substance on Food-borne Microorganisms from Commiphora molmol Engl. and Its Application to Food Products (몰약(Commiphora molmol Engl.)의 식중독 미생물 증식 억제 물질의 구조동정 및 식품적용)

  • Han, Ji-Sook;Shin, Dong-Hwa;Baek, Nam-In
    • Korean Journal of Food Science and Technology
    • /
    • v.33 no.4
    • /
    • pp.401-408
    • /
    • 2001
  • The ethanol extract and n-hexane fraction of Commiphora molmol Engl. showed minimum inhibitory concentration of 50 ppm and 25 ppm, respectively, on 5 strains of Listeria monocytogenes at $32^{\circ}C$. The purified substance, C3-3-2 fraction, was isolated by silica gel column and preparative thin layer chromatography from n-hexane fraction of Commiphora molmol Engl. The C3-3-2 fraction showed a strong bactericidal activity on 5 strains of L. monocytogenes at the concentration of 10 ppm in tryptic soy broth medium. At that concentration, the viable count was reduced $5{\sim}6$ log cycle from initial cell number. The n-hexane fraction of Commiphora molmol Engl. showed strong growth inhibition at the concentration of 25 ppm on Bacillus cereus and Staphylococcus aureus, at 50 ppm in broth on Salmonella enteritidis, and at 500 ppm on Vibrio parahaemolyticus. The purified antimicrobial substance, the C3-3-2 fraction, was identified as m-nonylphenol by on the basis of the $^1H-,\;^{13}C-NMR$ and EI/MS data. For the application test, the C3-3-2 fraction which was purely isolated from Commiphora molmol Engl. at 100 ppm were applied to minced Alaska pollack and ground beef at $32^{\circ}C$ and $5^{\circ}C$. The antimicrobial substances did not reduce L. monocytogenes ATCC 19113 at $32^{\circ}C$, while they reduced L. monocytogenes ATCC 19113 in viable number at $5^{\circ}C$. However, the antimicrobial effect of C3-3-2 fraction in food system was lower than that of broth condition.

  • PDF

Analytical method of aflatoxins in edible oil and infant-children foods (식용유지와 영유아식품 중 아플라톡신 분석방법)

  • Hu, Soo-Jung;Park, Seung-Young;Kim, Soon-Sun;Lee, Joon-Goo;Song, Ji-Young;Kang, Eun-Gi;Lee, Hyun-Sook;Cho, Dae-Hyun
    • Analytical Science and Technology
    • /
    • v.24 no.2
    • /
    • pp.150-157
    • /
    • 2011
  • Aflatoxins are secondary metabolites of the molds of Aspergillus flavus and Aspergillus parasiticus. They are highly carcinogenic compounds and can affect a wide range of vegetable commodities such as cereals (especially corn), nuts, peanuts, fruits and oil seeds, in the field and during storage. In fact, oilseeds are often stored for weeks in conditions that promote the mould growth, and the possible consequent presence of aflatoxins in oilseeds can lead to their transfer in oil. In addition, aflatoxins can be found as a natural contaminant in multi-cereals and beans making baby food for infants and young-children. The objective of this study was to validate the liquid extraction method or develop an analytical method for edible oil and infant-children foods. Therefore, this study developed condition of extract for aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) in edible oil using a high performance liquid chromatography with florescence detector (HPLC/FLD). Aflatoxins were extracted from edible oil samples by means of MSPD (Matrix solid phased dispersion), utilizing $C_{18}$ as dispersing material and purified by using immunoaffinity column. The gression line coefficients were above 0.999. The recoveries for aflatoxins ranged from 85.9 to 93.0%, and relative standard deviations were below 5.7%. The new developed method of aflatoxins effectively enhanced recoveries by using MSPD-Immunoaffinity column compared with liquid extraction. The analytical method for liquid extraction of aflatoxin was appropriate for infant-children food. Reviewing the current method, the recoveries of aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) were 89.5~92.3%.

Changes in the Physiological Activities of Four Sweet Potato Varieties by Cooking Condition (고구마 네 가지 품종의 조리방법에 따른 생리활성 변화)

  • Lee, Young-Min;Bae, Ji-Hyun;Kim, Jung-Bong;Kim, So-Young;Chung, Mi-Nam;Park, Mi-Young;Ko, Jeong-Sook;Song, Jin;Kim, Jae-Hyun
    • Journal of Nutrition and Health
    • /
    • v.45 no.1
    • /
    • pp.12-19
    • /
    • 2012
  • The present study was performed to investigate antioxidant, anticancer, and antimicrobial activities of four Korean sweet potato variaties and to identify the changes in these biological activities under different cooking conditions. Total polyphenol content was 3.8-73.6 mg/g in 80% ethanol extracts of sweet potatoes. The polyphenol content was highest Sinjami variety (p < 0.05). Radical scavenging activity against DPPH and $ABTS^{{\cdot}+}$ was high in Sinjami (p < 0.05) and the ethanol extract from Sinjami also showed effective superoxide dismutase (SOD)-like activity, which decreased significantly by steaming and roasting (p < 0.05). Ethanol extracts from the four sweet potato variaties did not inhibit cancer cell growth in MCF-7 or HepG2 cells at concentrations of 1, 10, and $100\;{\mu}g$/mL. Of the investigated sweet potato variaties, only Sinjami exhibited strong antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Salmonella typhimurium. The antimicrobial activity of Sinjami against E. coli, St. aureus, and S. typhimurium decreased following steaming and roasting (p < 0.05). These results indicate that the Sinjami Korean sweet potato had higher polyphenol content, radical scavenging activity, SOD-like activity, and antimicrobial activity than those of the other variaties and consuming raw Sinjami might be beneficial for maintenance of biological activities.

A study on the antioxidant activity of products of caramel-type-browning reaction (Caramel 갈색화 반응 생성물의 항산화성에 관한 연구)

  • 신민자;안명수
    • Korean journal of food and cookery science
    • /
    • v.16 no.6
    • /
    • pp.629-639
    • /
    • 2000
  • The study was carried out to compare the antioxidant activities of products from caramel-type-browning reaction of xylose(XY), glucose(GL), sucrose(SU), glucose + citric acid (GLCA), glucose + sodium citrate(GLSC), glucose + glycine(GLGC) heated at 80, 120 or 140$^{\circ}C$ for 24 hr. 1. The hydrogen donating ability (HDA) of browning reaction products was generally enhanced as the browning temperature and time increased. The HDAs of the browning reaction products heated at 80$^{\circ}C$ for 24 hr were in the order of GLSC (0.387) > GLSC (0.362) > GLCA (0.301) > GL (0.299) > XY (0.290) > SU (0.281). But they were in the order of GLSC (0.543) > SU (0.328) > GL (0.309) > GLGC (0.325) > XY (0.298) > GLCA (0.275) under the condition of heating at 140$^{\circ}C$ for 24 hr. 2. The antioxidant activities of the anhydrous ethanol extracts of the browning mixtures were inferior to that of TBHQ as measured in com oil, but SU was superior to tocopherol in its antioxidant activity. All the browning mixtures showed antioxidant activities when heated at 80$^{\circ}C$; however, only SU and GLCA showed the activites at 120 or 140$^{\circ}C$. And the antioxidant activity of the SU extract was higher than that of TOCO. The antioxidant activities of the ethanol extracts were in the order of TBHQ > GLCA > GLGC > TOCO > SU > XY > GL > GLSC > control at 80$^{\circ}C$, TBHQ > SU > TOCO > GLCA > control > GLSC> XY > GL > GLGC at 120$^{\circ}C$, and TBHQ > SU > TOCO > GLCA > control > GLSC > GLGC > XY > GL at 140$^{\circ}C$.

  • PDF

Relation between Growth Condition of Six Upland-Crops and Soil Salinity in Reclaimed Land (간척지에서 토양 염류와 6개 밭작물 생육과의 관계)

  • Lee, Seung-Heon;Hong, Byeong-Deok;An, Yeoul;Ro, Hee-Myong
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.36 no.2
    • /
    • pp.66-71
    • /
    • 2003
  • This study was carried out to obtain basic data for selecting the applicable crops in reclaimed land and to provide guidelines for the rotation between paddy and upland cropping. Field experiment was conducted with six summer crops(garland chrysanthemum, young radish, small radish, kale, lettuce, red lettuce) at Dae-Ho reclaimed experiment plots in Dangjin province. Dry weights and plant heights of harvested crops were measured and soil chemical properties were analyzed. Plant height and dry weight decreased significantly with increasing soil saturation paste extract electrical conductivity(ECe) and sodium adsorption ratio(SAR). The threshold ECe of salt inhibition for six crops was less than $1dS\;m^{-1}$ for young radish and kale, greater than $4dS\;m^{-1}$ for garland chrysanthemum, and greater than $6dS\;m^{-1}$ for small radish, lettuce, and red lettuce. At higher ECe that inhibits crop growth, with every increase in $1dS\;m^{-1}$, dry weight index decreased by 3.35 for kale, 3.92 for small radish, 3.98 for young radish, 4.66 for lettuce, 7.57 for garland chrysanthemum, and 8.45% for red lettuce, respectively. The ECe causing 50% reduction of dry weight index was $18.9dS\;m^{-1}$ for small radish, $17.3dS\;m^{-1}$ for lettuce, $15.4dS\;m^{-1}$ for kale, $12.0dS\;m^{-1}$ for red lettuce, $11.3dS\;m^{-1}$ for young radish, and $11.0dS\;m^{-1}$ for garland chrysanthemum. Among the tested 6 summer crops through field experiment and in-situ survey, kale was proved to be a favorable vegetable crop at reclaimed tidal land.

An analysis of horizontal deformation of a pile in soil using a beam-on-spring model for the prediction of the eigenfrequency of the offshore wind turbine (해상풍력터빈의 고유진동수 예측을 위한 지반에 인입된 파일의 탄성지지보 모델 기반 수평 거동 해석)

  • Ryue, Jungsoo;Baik, Kyungmin;Kim, Tae-Ryong
    • The Journal of the Acoustical Society of Korea
    • /
    • v.35 no.4
    • /
    • pp.261-271
    • /
    • 2016
  • In the prediction of response of a pile in soil, numerical approaches such as a finite element method are generally applied due to complicate nonlinear behaviors of soils. However, the numerical methods based on the finite elements require heavy efforts in pile and soil modelling and also take long computing time. So their usage is limited especially in the early design stage in which principal dimensions and properties are not specified and tend to vary. On the contrary, theoretical approaches adopting linear approximations for soils are relatively simple and easy to model and take short computing time. Therefore, if they are validated to be reliable, they would be applicable in predicting responses of a pile in soil, particularly in early design stage. In case of wind turbines regarded in this study, it is required to assess their natural frequencies in early stages, and in this simulation the supporting pile inserted in soil could be replaced with a simplified elastic boundary condition at the bottom end of the wind turbine tower. To do this, analysis for a pile in soil is performed in this study to extract the spring constants at the top end of the pile. The pile in soil can be modelled as a beam on elastic spring by assuming that the soils deform within an elastic range. In this study, it is attempted to predict pile deformations and influence factors for lateral loads by means of the beam-on-spring model. As two example supporting structures for wind turbines, mono pile and suction pile models with different diameters are examined by evaluating their influence factors and validated by comparing them with those reported in literature. In addition, the deflection profiles along the depth and spring constants at the top end of the piles are compared to assess their supporting features.

Regulatory Effect of Inflammatory Cytokines Secretion and Hypoxia-inducible $Factor-1{\alpha}$ Activation by Panax ginseng (인삼의 염증성 사이토카인 분비 및 저산소 유도인자-1${\alpha}$ 활성화 조절 효과)

  • Zo, Chul-Won;Lee, Seung-Hee;Kim, Dong-Woung;Lee, Seong-Kyun;Song, Bong-Keun
    • The Journal of Internal Korean Medicine
    • /
    • v.27 no.4
    • /
    • pp.864-878
    • /
    • 2006
  • Purpose : Panax ginseng(PG) is considered to have salutary effects and stimulant actions on physical capacity. However, the effects of PG on the inflammatory cytokine secretion and hypoxia condition are still not understood. This study wasto elucidate the effect of PG on inflammatory cytokine secretion such as interleukin (IL)-1, IL-6, granulocyte macrophage colony stimulating factor (GM-CSF), and tumor necrosis factor $(TNF)-{\alpha}$. Also, the effects on the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 (HIF-1) were measured. Methods : The water extract of PG was administrated to HMC-1 cells before phorbol myristate acetate (PMA)+A23187 treatment. $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, GM-CSF, and VEGF secretion were measured by a modified enzyme-linked immunosorbent assay (ELISA). HIF-1 activation was measured by transcription factor enzyme-linked immunoassay (TF-EIA) Results : PG significantly decreased secretion of $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, and GM-CSF in PMA+A23187-induced HMC-1 cells. VEGF secretion was not changed but HIF-1 activation was decreased by the treatment of PG. Conclusions : PG inhibited the secretion of inflammatory cytokines, which impliesPG might contribute to treatment of mast cell-mediated inflammatory disease. Also, PG inhibited PMA+A23187-induced $HIF -1{\alpha}activation}$ and DNA-binding activity for HIF-1. Therefore, these data demonstrate that PG modulates inflammatory cytokines through inhibition of $HIF-1{\alpha}activation}$ activation.

  • PDF

Screening for Effective Organic Farming Materials for the Control of Cucumber Scab Caused by Cladosporium cucumerinum (오이 검은별무늬병 방제에 효과적인 유기농업자재 선발)

  • Park, So-Hyang;Hong, Sung-Jun;Shim, Chang-Ki;Kim, Min-Jeong;Park, Jong-Ho;Han, Eun-Jung;Kim, Yong-Ki
    • Research in Plant Disease
    • /
    • v.23 no.2
    • /
    • pp.159-167
    • /
    • 2017
  • This study was conducted to evaluate the control efficacy of the organic farming materials on cucumber scab caused by Cladosporium cucumerinum PT1 (KACC 48094). The antifungal activities in vitro as well as the suppressive effect of 43 organic farming materials on the spore germination and germ tube growth by inoculating spore suspension on cucumber seedlings in vivo were investigated. Thirteen organic farming materials inhibited the mycelial growth of C. cucumerinum and nine of these were microbial agents. In the screening using cucumber seedlings, six organic farming materials were very effective with control efficacy value of 90%. Among them, Bacillus amyloliquefaciens M27 provided suppressive effect on both mycelial growth and spore germination against cucumber scab. Finally, nine organic farming materials were selected to test the protective and curative effects, and all chosen organic farming materials significantly suppressed disease incidence when applied in the preventive action, in comparison with the curative action. Especially, Bordeaux mixture I and III gave excellent protective control efficacy with control values of 96.7% and 73.3%, respectively, whereas its curative control effect was significant low. Among these, only Thymus quinquecostatus+Sophora extract showed curative activity, although the control value was as low as 50%. This study suggests that cucumber scab can be controlled by some organic farming materials in the farmhouses under comparatively cold and wet condition and protective treatment is more important and efficient.