Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Changes in the Physicochemical and Antioxidant Characteristics during the Fermentation of Jujube Wine Using Hot Water Extract of Dried Jujube

건대추 열수추출물을 이용한 대추와인 발효중의 이화학 및 항산화적 특성 변화

  • Eom, In-Ju (Department of Food Engineering, Daegu University) ;
  • Choi, Jung-In (Department of Food Engineering, Daegu University) ;
  • Kim, In-Ho (Department of Food Engineering, Daegu University) ;
  • Kim, Tae-Hoon (Department of Food Engineering, Daegu University) ;
  • Kim, Seong-Ho (Department of Food Engineering, Daegu University)
  • Received : 2016.06.29
  • Accepted : 2016.08.17
  • Published : 2016.11.30
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Abstract

In the study, we investigated the optimum fermentation conditions as well as changes of physicochemical and antioxidant characteristics during the fermentation of jujube wine. The physicochemical characteristics of the jujube hot water extracts used in this study were a pH of 5.05, 0.01% acidity, and $6.5^{\circ}Brix$ concentration. For jujube wine fermentation, the optimal fermentation strain was selected among the isolated strains and the final chosen strain was identified as Saccharomyces cerevisiae, based on the 26S rRNA gene sequencing and similarity searching in GenBank DB. The jujube wine fermented with an initial $15^{\circ}Brix$ concentration of jujube extracts showed a maximum alcohol content of 13% and lower residual sugar concentration. Alcohol content during the jujube wine fermentation was increased after 3 days of fermentation, and no significantly difference after 6 days was found. The residual sugar concentration during the fermentation periods was significantly decreased with increasing alcohol content. The jujube wine properties at 12 days of fermentation were as follows: a pH of 4.34, acidity of 0.29%, alcohol content of 12.8%, and a residual sugar concentration of $8.70^{\circ}Brix$. The malic acid content in the organic acid of fermented jujube wine was significantly decreased during the fermentation proceeding, whereas the succinic acid and lactic acid contents were significantly increased. Antioxidant characteristics of the fermented jujube wine were appeared ABTS radical scavenging activity 45.80%, DPPH radical scavenging activity 61.89%, nitrite scavenging activity 91.95% and total polyphenol compound 3.69 mg/ml. In terms of consumer liking of the jujube wine by sensory evaluation, the color and overall acceptability of jujube wine were evaluated as more than average.

본 연구는 대추 열수 추출액을 이용한 대추 와인의 발효중 이화학적, 항산화적 특성 변화 및 최적발효조건을 조사하였다. 실험에 사용된 대추 열수 추출물의 이화학적 특성으로는 pH 5.05, 산도 0.01% 및 농도 $6.5^{\circ}Brix$이었다. 대추 와인의 발효를 위한 최적의 발효 균주가 최종 선별되었고 최종 선정된 1균주는 26S rRNA gene sequencing과 GenBank DB의 유사성 분석에 의하여 Saccharomyces cerevisiae로 동정 되었다. 대추 와인 발효를 위한 추출액의 초기 농도 $15^{\circ}Brix$에서 알코올 함량은 13%로 가장 높았고 잔당 함량은 가장 낮은 값을 보였다. 대추 와인발효 기간중의 알코올 함량은 3일째부터 급격한 증가가 있었고 발효 6일 이후는 유의적인 차이가 없었으며, 잔당 함량은 알코올 함량의 증가와 더불어 유의적으로 감소하였다. 발효 12일째 대추 와인의 pH는 4.34, 산도는 0.29%, 알코올 함량은 12.8%, 당도는 $8.70^{\circ}Brix$로 조사되었다. 대추 와인의 유기산 함량은 malic acid의 경우, 발효가 진행됨에 따라 유의적으로 감소하는 경향을 보였고 succinic acid와 lactic acid는 유의적으로 증가하는 것으로 나타났다. 대추 와인의 항산화능 특성 중 ABTS는 45.80%, DPPH은 61.89%, 아질산염 소거활성능은 91.95%이었고, total polyphenol은 3.69 mg/ml의 값을 보였다. 대추 와인의 관능적 평가에 의한 소비자 기호도에서 색과 전체적인 기호도면에서 평균 이상으로 평가되었다.

Keywords

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