• Title/Summary/Keyword: external magnetic field

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Detection of Fish Virus by Using Immunomagnetic Separation and Polymerase Chain Reaction (IMS-PCR)

  • KIM Soo Jin;OH Hae Keun;CHOI Tae-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.6
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    • pp.948-955
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    • 1997
  • Immunomagnetic separation of virus coupled with .reverse transcription-polymerase chain reaction (IMS-PCR) was performed with infectious hematopoietic necrosis virus (IHNV). A DNA fragment of expected size was synthesized in the RT-PCR with total RNA extracted from IHNV inoculated CHSE-214. In a SDS-PAGE analysis, a protein band of over 70kDa was detected from non-infected cells and cells inoculated with IHNV and infectious pancreatic necrosis virus (IPNV). This protein was detected in the Western blot analysis probably because of non-specific reaction to monoclonal antibody against IHNV nucleocapsid protein. In the immunomagnetic separation, magnetic beads coated with monoclonal antibody against the IHNV nucleocapsid protein was incubated with supernatant from IHNV inoculated CHSE-214 cells. During this process, the non-specifically reacting protein could be removed by washing the magnetic bead with PBS in the presence of an external magnetic field, and viral proteins were detected from the remaining, cleaned magnetic beads. It was necessary to extract viral RNA from the captured virus particles before RT-PCR, and no DNA product was detected when the captured virus was only heated 5 min at $95^{\circ}C$. A PCR-product of expected size was synthesized from IMS-PCR with magnetic beads double coated either by goat anti-mouse IgG antibody -monoclonal antibody or streptavidin - biotin conjugated monoclonal antibody.

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Observation of the Domain Structures in Soft Magnetic (Fe97A13)85N15/Al2O3 Multilayers

  • Stobiecki, T.;Zoladz, M.
    • Journal of Magnetics
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    • v.8 no.1
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    • pp.13-17
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    • 2003
  • The longitudinal magnetooptical Kerr effect was used to analyse magnetic domains in soft magnetic ${(Fe_{97}A1_3)}_{85}N_{15}$/$Al_{2}O_{3}$ multilayers in order to get microscopic understanding of interlayer exchange coupling. The measuring system consists of a Kerr microscope, a CCIR camera (with an 8-bit framegrabber), 16 bit digital camera and computer system for real-time image processing and to control external magnetic field and cameras. The strength of ferromagnetic (EM) coupling as a function of the spacer thickness of $Al_2O_3$ was investigated. It was found that strong FM-coupling, strong uniaxial anisotropy and coherent rotation of the magnetization have been observed for the spacer thickness in the range of 0.2 nm $\leq$ t $\leq$ 1 m, however, weak FM-coupling, patch domains and $360^{\circ}$-walls occur for the spacer thickness of t = 2.5 nm. At a spacer thickness of t $\geq$ 5 nm transition takes place from weak FM-coupling to the decoupled state where complex interlayer interactions and different types of the domain walls were observed.

Formation of Magnetic Graphene Nanosheets for Rapid Enrichment and Separation of Methyl Orange from Water

  • Zhang, Feng-Jun;Zhang, Zhuo;Xie, Fa-Zhi;Xuan, Han;Xia, Hong-Chen;Zhu, Lei;Oh, Won-Chun
    • Journal of the Korean Ceramic Society
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    • v.51 no.6
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    • pp.570-574
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    • 2014
  • Magnetic-graphene nanosheets have been synthesized via a simple effective chemical precipitation method followed by heat treatment. The composite nanosheets are super paramagnetic at room temperature and can be separated by an external magnetic field. The prepared magnetic-graphene nanosheets were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, and BET surface area analysis. The results demonstrated the successful attachment of iron oxide nanoparticles to graphene nanosheets. It was found that the attached nanoparticles were mainly $Fe_3O_4$. The magnetic-graphene nanosheets showed near complete methyl orange removal within 10 mintues and would be practically usable for methyl orange separation from water.

Design and Preparation of Magnetic CTAB/Montmorillonite Nanocomposite for Phenols Removal

  • Shen, Rong;Yu, Yichang;Wang, Yue;Xia, Zhining
    • Nano
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    • v.13 no.10
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    • pp.1850123.1-1850123.9
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    • 2018
  • The cetyltrimethyl ammonium bromide (CTAB)-modified montmorillonite (MMT) was synthesized via a novel "dissolution and reassembly" method. To determine the optimal formula, the adsorption of C.I. Reactive Red 2 (X3B) with CTAB/MMT was investigated. The optimal CTAB/MMT nanocomposite was used to remove 2,6-dichlorophenol and p-nitrophenol from aqueous solutions. The adsorption results can be described by Langmuir isotherm, and the adsorption capacities were 200 mg/g and 125 mg/g for 2,6-dichlorophenol and p-nitrophenol, respectively. To realize the quick separation and recycle, the magnetic CTAB/MMT was further strategized and synthesized. The adsorption equilibrium time was 15 min for both contaminants; the ions' strength showed a little bit of influence on the adsorption performance. In addition, compared with acidic condition, neutral condition was more beneficial to the adsorption reaction. Due to the addition of $Fe_3O_4$, the adsorption capacities of this magnetic nanocomposite for 2,6-dichlorophenol and p-nitrophenol were a little bit decreased, which were 170 mg/g and 91 mg/g, respectively. However, the magnetic nanocomposite can be separated within 30 s under an external magnetic field, which would be useful in the practical application.

Development of Magnetic Sensor for Measurement of the Cable Tension of Large Scale Bridge (대형교량 케이블 장력 측정을 위한 자기센서 개발)

  • Park, Hae-Won;Ahn, Bong-Young;Lee, Seung-Seok;Kim, Jong-Woo
    • Journal of the Korean Society for Nondestructive Testing
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    • v.27 no.4
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    • pp.339-344
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    • 2007
  • Safety of large scale cable in bridge is very important because it may cause the unwanted catastrophic failure. Although the proof load were considered at the design stage, its soundness must be monitored continuously because the cable may be broken out without warning by the variable external load. The cable tension of in-use structures has been mainly measured by the resonance method and its use has been limited because of relatively large measurement uncertainty. Recently a new magnetic method was developed and its reliability is known to be good for evaluating the cable tension. In this study a system which can deliver the calibrated load to the cable was developed and the measurement reliability of developed magnetic sensor according to the change of external load was analyzed quantitatively. The effect of magnetization frequency, bias magnetic field, and temperature on the sensor output was also evaluated.

Numerical Study of Entropy Generation with Nonlinear Thermal Radiation on Magnetohydrodynamics non-Newtonian Nanofluid Through a Porous Shrinking Sheet

  • Bhatti, M.M.;Abbas, T.;Rashidi, M.M.
    • Journal of Magnetics
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    • v.21 no.3
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    • pp.468-475
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    • 2016
  • In this article, entropy generation on MHD Williamson nanofluid over a porous shrinking sheet has been analyzed. Nonlinear thermal radiation and chemical reaction effects are also taken into account with the help of energy and concentration equation. The fluid is electrically conducting by an external applied magnetic field while the induced magnetic field is assumed to be negligible due to small magnetic Reynolds number. The governing equations are first converted into the dimensionless expression with the help of similarity transformation variables. The solution of the highly nonlinear coupled ordinary differential equation has been obtained with the combination of Successive linearization method (SLM) and Chebyshev spectral collocation method. Influence of all the emerging parameters on entropy profile, temperature profile and concentration profile are plotted and discussed. Nusselt number and Sherwood number are also computed and analyzed. It is observed that entropy profile increases for all the physical parameters. Moreover, it is found that when the fluid depicts non-Newtonian (Williamson fluid) behavior then it causes reduction in the velocity of fluid, however, non-Newtonian behavior enhances the temperature and nanoparticle concentration profile.

Josephson Vortex Dynamics in Tilted Magnetic Fields (경사 자기장 하에서의 조셉슨 볼텍스 동역학)

  • Jin, Yong-Duk;Ki, Dong-Keun;Lee, Hu-Jong
    • Progress in Superconductivity
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    • v.9 no.2
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    • pp.140-145
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    • 2008
  • We report on the Josephson vortex dynamics in $Bi_2Sr_2CaCuO_{8+\delta}$ natural Josephson junctions by c-axis tunneling measurements. Beside the quasiparticle branches in the current-voltage characteristics, a new set of multiple branches, referred to as Josephson-vortex-flow branches (JVFBs), are observed. The JVFBs emerge in an in-plane magnetic field above $H_0\;=\;{\Phi}_0/{\gamma}s^2$ and show highly hysteretic behavior, which can be explained in terms of the recently proposed dynamic-phase-separation model. In this work we examined the effect on the JVFBs by the presence of pancake vortices generated as the external magnetic field was applied slightly tilted from the in-plane direction. JVFBs were found to become larger and prominent with increasing pancake vortex density as the tilt angle increased, which were presumably caused by slowing down of a Josephson vortex lattice in the presence of pancake vortices.

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Effect of Cooling-rate Dependence on the Magnitude of Thermoremanent Magnetization (냉각률이 자화에 미치는 영향)

  • Yu, Yong-Jae
    • 한국지구물리탐사학회:학술대회논문집
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    • 2010.10a
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    • pp.43-45
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    • 2010
  • Acquisition of thermoremanent magnetization follows a Boltzman statistics, as such long reaction time in a slowly cooled environment allows more chance to align individual magnetic particles parallel to the external magnetic field. Hence it has been proposed that the slowly cooled rocks often acquire stronger magnetization than the rapidly cooled ones. Such a proposition has been experimentally validated to be true for the fine-grained magnetite- or titanomagnetite bearing basaltic rocks collected from the mid-ocean ridges. However, the effect of cooling-rate on the remanence intensity appears to be insignificant for nominal grain ranges.

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The Domestic Development of a Superconducting MRI Magnet (초전도 MRI 마그네트 국산화 개발)

  • 배준한;심기덕;고락길;진홍범;조전욱;하동우;오상수;권영길;류강식
    • Proceedings of the Korea Institute of Applied Superconductivity and Cryogenics Conference
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    • 2001.02a
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    • pp.121-124
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    • 2001
  • The research results on the superconducting magnet for whole body MRI are presented. The magnet consists of main coil with 6 solenoid coils, shielding coil with 2 solenoid coils and 6 sets of cryogenic shim coil. The ferromagnetic shim assembly is installed on the inside wall of the room temperature bore for shimming inhomogeneous field components generated due to manufacturing tolerances, installation misalignments and external ferromagnetic materials near the magnet. Also, the magnet is enclosed with the horizontal type cryostat with 80cm room temperature bore to keep the magnet under the operating temperature. The magnetic field distributions within the imaging volume were measured by the NMR field mapping system. Through the test, the central field of magnet was 1.5 Tesla and the field homogeneity of 9.3 ppm has been obtained on 40cm DSV(the diameter of spherical volume) and using this magnet, comparatively good images for human body, fruits and water phantoms have been achieved.

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Magnetic Properties of the $Y_{1}Ba_{2}Cu_{3-x}Sn_{x}O_{7-y}$ $High-T_{C}$ Superconductor (고온 초전도체 $Y_{1}Ba_{2}Cu_{3-x}Sn_{x}O_{7-y}$의 자기적 특성 연구)

  • 이성규;이주일;김문석;유성초;임우영;백종성
    • Journal of the Korean Magnetics Society
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    • v.1 no.2
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    • pp.15-21
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    • 1991
  • The magnetic properties of the $Y_{1}Ba_{2}Cu_{3-x}Sn_{x}O_{7-y}$ superconductor were studied as a function of Sn concentration by utilizing both the vibrating sample magnetometer (VSM) and torque magnetometer. Unlike the cases where Fe and Co were substituted for Cu, the superconducting transition temperature was maintained above 90 K until x reached the value of 0.36. The lower critical field $H_{c1}(T)$ and upper critical field $H_{c2}(T)$ are measured as a function of temperature and external magnetic field, respectively. By aid of these results, $H_{c1}(0)$.($H_{c2}(0)$), the coherence length ${\varepsilon}_{0}$, the penetration depth ${\lambda}_{0}$, and the Ginzburg-Landau parameter k were oqtained. Flux pinning was also observed in the sample.

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