• International Journal of Industrial Entomology and Biomaterials
• /
• v.15 no.1
• /
• pp.83-85
• /
• 2007

A glycine and proline-rich antibacterial protein was cloned from larvae of a beetle, Protaetia brevitarsis. The DNAs encoded a deduced propeptide of 127 amino acid residues with predicted molecular weight of 14.0 kDa and PI of 7.89. Structural analysis of this protein indicated the presence of a recognition sequence for the cleavage site within the constitutive secretory pathway(Arg-Xaa-Lys/Arg-Arg), suggesting that mature portion(72 amino acid residues) is produced by cleavage of signal peptide and propeptide from 127 amino-acid-long precursor protein. Mature portion sequence of this protein showed 72% similarity to that of Oryctes rhinoceros Rhinocerosin and 91% to that of Holotrichia diomphalia holotricin 2. The mRNA expression was reached the highest level at 4 hrs after E. coli injection and then declined gradually.

• Journal of Internet Computing and Services
• /
• v.15 no.3
• /
• pp.11-19
• /
• 2014

The natural user interface/experience (NUI/NUX) is used for the natural motion interface without using device or tool such as mice, keyboards, pens and markers. Up to now, typical motion recognition methods used markers to receive coordinate input values of each marker as relative data and to store each coordinate value into the database. But, to recognize accurate motion, more markers are needed and much time is taken in attaching makers and processing the data. Also, as NUI/NUX framework being developed except for the most important intuition, problems for use arise and are forced for users to learn many NUI/NUX framework usages. To compensate for this problem in this paper, we didn't use markers and implemented for anyone to handle it. Also, we designed multi-modal NUI/NUX framework controlling voice, body motion, and facial expression simultaneously, and proposed a new algorithm of mouse operation by recognizing intuitive hand gesture and mapping it on the monitor. We implement it for user to handle the "hand mouse" operation easily and intuitively.

• Journal of Sasang Constitutional Medicine
• /
• v.25 no.4
• /
• pp.330-342
• /
• 2013

Objectives This study was to examine the differences on alexithymia, rumination, depression and somatic symptoms by each Sasang constitution, and aimed at combining the psychological construct with Korean medical treatment. Methods One hundred eighty men and women aged from 20 to 69 who reside in Geoje-si were selected, and data from 173 people consisting of the three constitutions-Soyangin(39), Taeeumin(83), and Soeumin(51), excepted the minority sample of Taeyangin(7 people)-were analyzed. Collected data were analyzed and tested by IBM SPSS Statistics 20: frequency analysis for demographic data; t-test for gender differences; one-way ANOVA for differences among three constitutions; and Pearson correlation analysis for the correlation between variables. Results The result of t-test taken for the gender differences between variables was not significant. According to the result of one-way ANOVA conducted by different Sasang constitutions, no variables showed a significant result except that of alexithymia. The correlation analysis showed that the alexithymia had positive correlation with rumination, depression, and somatic symptoms. Rumination was in positive correlation with depression and somatic symptoms. Especially, depression had high positive correlation with somatization symptoms. Among the variables analyzed by each constitution, only the survey result of the alexithymia was significant. Conclusions Given that each constitution showed different levels of the alexithymia, it can be concluded that therapeutic intervention in the treatment for the Taeeumin who shows a high level of alexithymia is needed to improve their emotional recognition and expression. In this context, application of program which focus on the emotional ability such as emotion coaching to the Korean medical treatment is expected to improve alexithymia, depression, and somatic symptoms.

• IMMUNE NETWORK
• /
• v.7 no.1
• /
• pp.26-30
• /
• 2007

Background: Mycobacterial antigens released as PIM, LM, LAM, lipoproteins and other cellular factors may contribute to macrophage and dendritic cell activation through pattern recognition receptors such as TLRs. In this study, we assessed cytokine production and ERK activation with stimulation of several major mycobacterial antigens. Methods: Purified mycobacterial antigens (10, 22, 30, 38kDa) and recombinant antigens (6, 16, 19, 38kDa, Ag85A antigen) were studied. The production of cytokines (TNF-${\alpha}$, IL-12, IL-6) was measured by ELISA. The ERK activation was detected by western blotting. The expression of TLR2 or TLR4 was measured by flow cytometry. Results: Among purified antigens only 30kDa antigen induced production of IL-6 or TNF-${\alpha}$ in THP-1 macrophage cells. When THP-1 macrophage cells were treated with 30kDa antigen, phosphorylation of ERK was detected. ERK activation also occurred in TLR2 transfectant HEK293 cells with 30kDa antigen stimulation. Conclusion: 30kDa antigen is one of the major mycobacterial antigens inducing cytokine production and MAP kinases phosphorylation in macrophages.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.6
• /
• pp.3479-3482
• /
• 2013

Background: Proliferation markers widely have been used to diagnose and determine the behaviour and prognosis of benign and malignant tumours. Minichromosome maintenance 3 (MCM3) is a novel proliferation marker. The aim of this study was to evaluate and compare MCM3 with Ki-67 in diagnosis of salivary gland tumours. Materials and Methods: In this retrospective study, immunohistochemical expression of MCM3 and Ki-67 was evaluated in 15 pleomorphic adenomas (PA), 17 mucoepidermoid carcinomas (MEC) and 18 adenoid cystic carcinomas (ADCC). Labeling indices (LIs) for the two markers were calculated and compared. Results: MCM3 and Ki-67 LIs were significantly higher in MEC and ADCC compared to PA. The LI of MCM3 was significantly higher than that of Ki-67 in MEC and PA. There was no significant difference between the two markers in ADCC. A cut-off point of 8% with 74.3% sensitivity and 93.3% specificity for MCM3 was obtained to discern between benign and malignant tumors. Conclusions: These results suggest that MCM3 might be a useful proliferation marker for differential diagnosis and recognition of clinical behavior of salivary gland tumors.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.20 no.7
• /
• pp.1317-1324
• /
• 2016

Utilizing the Arduino platform to express the emotions that reflect the colors expressed the jewelry. Emotional color expression utilizes Plutchik's Wheel of Emotions model was applied to the similarity of emotions and colors. It receives the recognized value from the temperature, lighting, sound, pulse sensor and gyro sensor of a smart jewelery that can be easily accessible from your smartphone processes that recognize and process the emotion applied the rules of inference based on ontology. The emotional feelings color depending on the color looking for the emotion seen in context and applied to the smart LED jewelry. The emotion and the color combination of contextual information extracted from the recognition sensors are reflected in the built-in smart LED Jewelry depending on the emotions of the wearer. Take a light plus the emotion in a smart jewelery can represent the emotions of the situation, the doctor will be able to be a tool of representation.

• Science of Emotion and Sensibility
• /
• v.11 no.2
• /
• pp.173-180
• /
• 2008

In this research, experiments were performed to focus on haptical factors among the emotional way of expression in design, and, find out what factors of preference influenced by haptic elements as surface material, grip senses and shape that considering clues when consumer has purchase intention. The questionnaire survey of beverage bottles in experiment extract to haptic elements were estimated in various surface of bottles and consumer preference through practical touch by managed objects themselves. The experimental groups for this survey, one is using sight and sense of touch both, the other groups are use sense of touch only without sense of sight. Therefore, to find out effective factors for design and functional matter as the kind of treating surface and properties derived from comparing two experimental groups have recognition in level of priority for each element related with haptical design sources. Finally, considering practical use schema with emotional approach based on haptical property to adopting design process.

• Biomedical Science Letters
• /
• v.11 no.4
• /
• pp.487-492
• /
• 2005

The baculovirus/insect cell expression system is of great value in the study of structure-function relationships in mammalian glucose-transport proteins by site-directed mutagenesis and for the large-scale production of these proteins for mechanistic and biochemical studies. Spodoptera frugiperda Clone 21 (Sf2l) cells grow well on TC-100 medium that contains $0.1\%$ D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transporters. However, very little is known about the properties of the endogenous sugar transporter(s) in Sf2l cells, although a saturable transport system for hexose uptake has been previously revealed in the Sf cells. In order to further examine the substrate and inhibitor recognition properties of the Sf2l cell transporter, the ability of hexoses to inhibit 2-deoxy-D-glucose (2dGlc) transport was investigated by measuring inhibition constants $(K_i)$. The $K_i's$ for reversible inhibitors were determined from plots of uptake versus inhibitor concentration. Transport was effectively inhibited by D-mannose and D-glucose. Of the hexoses tested, L-glucose had the least effect on 2dGlc transport in the Sf2l cells, indicating that the transport is stereoselective. Unlike the human HepG2 type glucose transport system, D-mannose had a somewhat greater affinity for the Sf2l cell transporter than D-glucose, implying that the hydroxyl group at the C-2 position is not necessary for strong binding. However, epimerization at the C-4 position of D-glucose (D-galactose) resulted in a dramatic decrease in affinity of the hexose for the Sf2l cell transporter. Such a lowering of affinity might be the result of the involvement of the C-4 hydroxyl in hydrogen bonding. It is therefore suggested that Sf2l cells were found to contain an endogenous sugar transport activity that in several aspects resembles the human HepG2 type glucose transporter, although the insect and human transporters do differ in their affinity for cytochalasin B.

• Journal of the Korea Convergence Society
• /
• v.11 no.10
• /
• pp.207-212
• /
• 2020

This study analyzes the Soju(Korean alcohol) advertisement image, which is relatively easy to interpret subjectively, among sexual appeal advertisements that stimulate consumers' curiosity, where the image is verified through AOI (area of interest) 3 areas (face, body, product), and eye-tracking, one of the psychophysiological indicators. The result of the analysis reveals that visual attention, the interest in the advertising model, was higher in the face than in the body shape. Contrary to the prediction that men would be more interested in body shape than women, both men and women showed higher interest in the face than a body. Besides, it was derived that recognition and recollection of the product were not significant. This study is significant in terms of examining the pattern of visual attention such as the gaze point and gaze time of male and female consumers on sexual appeal advertisements. In further, the study looks forward to bringing a positive influence to the soju advertisement image by presenting the expression method that the soju advertisement image should pursue as well as the appropriate marketing direction.

• The KIPS Transactions:PartB
• /
• v.17B no.5
• /
• pp.355-362
• /
• 2010

Face tracking is to estimate the motion of a non-rigid face together with a rigid head in 3D, and plays important roles in higher levels such as face/facial expression/emotion recognition. In this paper, we propose an AAM-based face tracking algorithm. AAM has been widely used to segment and track deformable objects, but there are still many difficulties. Particularly, it often tends to diverge or converge into local minima when a target object is self-occluded, partially or completely occluded. To address this problem, we utilize the scale invariant feature transform (SIFT). SIFT is an effective method for self and partial occlusion because it is able to find correspondence between feature points under partial loss. And it enables an AAM to continue to track without re-initialization in complete occlusions thanks to the good performance of global matching. We also register and use the SIFT features extracted from multi-view face images during tracking to effectively track a face across large pose changes. Our proposed algorithm is validated by comparing other algorithms under the above 3 kinds of occlusions.