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The Korean Association of Immunobiologists (대한면역학회)
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Production of TNF-${\alpha}$ and IL-6 in Macrophages by Mycobacterial Protein Antigens

결핵균 단백항원 자극에 의한 대식세포의 TNF-${\alpha}$ 및 IL-6 생성과 ERK 활성화

  • Ahn, Hae-Jeong (Department of Microbiology, Yonsei University College of Medicine) ;
  • Cho, Sang-Nae (Department of Microbiology, Yonsei University College of Medicine) ;
  • Paik, Tae-Hyun (Department of Microbiology, College of Medicine, Konyang University) ;
  • Lee, Jung-Lim (Department of Microbiology, College of Medicine, Konyang University) ;
  • Choi, In-Hong (Department of Microbiology, Yonsei University College of Medicine)
  • 안혜정 (연세대학교 의과대학 미생물학교실) ;
  • 조상래 (연세대학교 의과대학 미생물학교실) ;
  • 백태현 (건양대학교 의과대학 미생물학교실) ;
  • 이정림 (건양대학교 의과대학 미생물학교실) ;
  • 최인홍 (연세대학교 의과대학 미생물학교실)
  • Published : 2007.03.30
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Abstract

Background: Mycobacterial antigens released as PIM, LM, LAM, lipoproteins and other cellular factors may contribute to macrophage and dendritic cell activation through pattern recognition receptors such as TLRs. In this study, we assessed cytokine production and ERK activation with stimulation of several major mycobacterial antigens. Methods: Purified mycobacterial antigens (10, 22, 30, 38kDa) and recombinant antigens (6, 16, 19, 38kDa, Ag85A antigen) were studied. The production of cytokines (TNF-${\alpha}$, IL-12, IL-6) was measured by ELISA. The ERK activation was detected by western blotting. The expression of TLR2 or TLR4 was measured by flow cytometry. Results: Among purified antigens only 30kDa antigen induced production of IL-6 or TNF-${\alpha}$ in THP-1 macrophage cells. When THP-1 macrophage cells were treated with 30kDa antigen, phosphorylation of ERK was detected. ERK activation also occurred in TLR2 transfectant HEK293 cells with 30kDa antigen stimulation. Conclusion: 30kDa antigen is one of the major mycobacterial antigens inducing cytokine production and MAP kinases phosphorylation in macrophages.

Keywords

References

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