• Clinical and Experimental Reproductive Medicine
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• v.23 no.1
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• pp.25-32
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• 1996

This work has been carried out to examine the number of Total, ICM and TE cells of F1 mouse blastcysts at day 4 after IVF by differential labelling of the nuclei with polynucleotide-specific fluorochromes and to obtain a fundamental information of preimplantation mouse embryo development. Blastocysts produced by superovulated B6CBA F1(C57BL/${\times}$CBA) eggs were inseminated with $1{\times}10^6$spermatozoa/ml and cultured in M16 medium at $37^{\circ}C$, 5% $CO_2$ incubator for 95hrs. Blastocysts were classified as early, middle, expanded and hatching stage according to the developmental morphology; blastocoel expansion and zona thickness. The results obtained in these experiments were summarized as follows; 1) The development rate of blastocysts at 95hrs after IVF was 86.7% and classified blastocysts to early, middle, expanded and hatching were 16.3%, 18.9%, 10.5% and 40.9%, respectively. 2) The numbers of total blastomere using bisbenzimide in the classified blastocysts to early, middle, expanded and hatching were 35.6${\pm}$1O.4, 49.4${\pm}$8.6, 60.8${\pm}$1O.7 and 62.7${\pm}$13.9, respectively. 3) In ICM and TE cell number by using differential labelling with polynucleotide-specific fluorochrome in the classified blastocysts to early, middle, expanded and hatching; ICM numbers were 9.6${\pm}$3.0, 13.6${\pm}$3.9, 16.0${\pm}$3.3 and 19.5${\pm}$4.6, respectively and TE cell numbers were 30.6${\pm}$5.1, 39.9${\pm}$5.8, 42.2${\pm}$8.1 and 43.7${\pm}$11.1, respectively. These results showed the same increase pattern according to development advance level. Also, when compared with the results of total count were obtained between bisbenzimide only and differential labelling, both of them showed the same increase pattern according to development level and at the same time their cell numbers were almost the same. So, rapid and simple cell count method using differential labelling can be used for the examination of later preimplantation development or as an indicator of embryo quality according to the variables of culture conditions.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 2000.04a
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• pp.157-162
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• 2000

Discriminant analysis is a method to relate whether the objects have a specific characteristic or not with their 'continuous' attribute values and, for given objects, to estimate whether they have a specific characteristic or not by their values of discriminant scores gotten from their attribute values. The author developed the new 'computational' method of discriminant analysis without specific hypotheses or assumptions and, by this new method, we can find 'feasible' solutions under the conditions required by our actual problems. In this paper, the author tried to apply this new method to the discrimination of logical relations. If this trial could be a success, we can apply this new method of discriminant analysis to the problems about relating the specific characteristic of the objects with their 'discrete' attribute values. The result was successful and the applicability of discriminant analysis could be expanded as a method for constructing the models for "estimating impressions".

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.23 no.4
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• pp.283-300
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• 2019

In this paper, we propose a new semi-analytic approach based on the generalized Taylor series for solving nonlinear differential equations of fractional order. Assuming the solution is expanded as the generalized Taylor series, the coefficients of the series can be computed by solving the corresponding recursive relation of the coefficients which is generated by the given problem. This method is called the generalized differential transform method(GDTM). In several literatures the standard GDTM was applied in each sub-domain to obtain an accurate approximation. As noticed in [19], however, a direct application of the GDTM in each sub-domain loses a term of memory which causes an inaccurate approximation. In this work, we derive a new recursive relation of the coefficients that reflects an effect of memory. Several illustrative examples are demonstrated to show the effectiveness of the proposed method. It is shown that the proposed method is robust and accurate for solving nonlinear differential equations of fractional order.

• Journal of the Korean Society for Railway
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• v.6 no.3
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• pp.194-202
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• 2003

To make railway carbody light in weight has advantages at some aspects of both manufacturing and maintenance. Recently, railway carbodys of steel structure have been lightened their weight by using aluminum extrusion plate. for the additional lightening of railway carbody, an optimal design which maintains proper strength and minimizes weight must be achieved. Optimization which is used with finite element analysis for aluminum extrusion plate has the disadvantage of consuming much time. In this paper, the method of equivalent material property which is available to FEA code is established using the method of equivalent stiffness. This method for plate is expanded into the method for railway carbody structure with plates and shells. An objective function is established for maximum stiffness of unit aluminum extrusion plate using established method of equivalent material property. We performed an multi-objective optimization using the penalty function method. As a result, recommendable shapes and sizes of unit extrusion plate for under-frame of high speed train is presented.

• The Plant Pathology Journal
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• v.17 no.6
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• pp.315-324
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• 2001

Tobacco (Nicotiana tabacum cvs.Xanthi-nc and NC 82) plants infected with Tobacco mosaic virus (TMV) were examined ultrastructurally. Local lesions produced by TMV were sunken and withered. The plants were subjected to temperature shift (TS), a method to produce programmed cell death (PCD), by placing the infected plants initially at high temperature (35$^{\circ}C$) for 2 days and then shifting them to greenhouse temperature (22-27$^{\circ}C$). As a result, expanded lesions around the original necrotic lesions were produced. The expanded area initially had no symptoms, but it withered and became necrotic 15 h after TS. No ultrastructural changes related to PCD were noted at 0 h after TS in Xanthi-nc tobacco tissues as well as in healthy and susceptible tobacco tissues infected with TMV, At 6 h after TS, chloroplasts were convoluted and cytoplasm began to be depleted; however no necrotic cells were found. At 17 h after TS, ground cytoplasm of affected cells was completely depleted and chloroplasts were stacked together with bent cell wall or dispersed in the intracellular space. Necrotic cells were also observed, containing virus particles in the necrotic cytoplasm. There were initially two types of symptoms in the expanded lesions: chlorosis and non-chlorosis (green). Abundant TMV particles and X-bodies were only found in the chlorotic tissue areas. These results suggest that PCD by TMV infection may start with the wilting of cells and tissues before necrotic lesion formation.

• Geomechanics and Engineering
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• v.22 no.6
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• pp.475-488
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• 2020

The recent increase in the use of Expanded Polystyrene (EPS) geofoam in construction and geotechnical projects has driven researchers to investigate its behavior, more deeply. In this paper, a series of experimental tests to investigate the stress-strain behavior and the mechanical properties of EPS blocks, under monotonic axial loading are presented. Four different densities of cylindrically shaped EPS with different dimensions are used to investigate the effects of loading rate, height and diameter, as well as the influence of the density of EPS on the stress-strain response. The results show that increasing the height of the EPS samples leads to instability of the sample and consequent lower resistance to the applied pressure. Large EPS samples show higher Young's modulus and compressive resistance due to some boundary effects. An increase in the rate of loading can increase the elastic moduli and compressive resistance of the EPS geofoam samples, which also varies depending on the density of the samples. It was also determined that the elastic modulus of EPS increases with increasing EPS density. By implementing an efficient numerical procedure, the stress-strain response of EPS geofoam samples can be reproduced with great accuracy. The numerical analysis based on the proposed method can used to evaluate the effect of different factors on the behavior of EPS geofoam.

• Korean Journal of Veterinary Research
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• v.38 no.2
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• pp.394-400
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• 1998

Cryopreservation of embryos by vitrification is a simple method to preserve bovine embryos for subsequent embryo transfer, but embryonic viability after vitrification has been inconsistent and low compared with conventional slow freezing. The aim of the present study is to examine the effect of serum or serum albumin in a vitrification solution and epidermal growth factor(EGF) or fibroblast growth factor(FGF) on in vitro viability of bovine blastocysts frozen by vitrification. Bovine blastocysts were produced by in vitro maturation, fertilization of follicular oocytes and culture of embryos in a synthetic oviduct fluid medium(SOFM) containing BSA and 19 essential and nonessential amino acids. Blastocysts with excellent or good morphology were selected at 7 or 8 days after culture and utilized for vitrification. In experiment 1, blastocysts were vitrified in a solution containing semi-fetal calf serum(SFCS) or BSA(5 or 10mg/ml) and then their subsequent viabilities were examined by culturing thawed embryos in a SOFM containing BSA and 19 amino acids. Effect of EGF or FGF added to a SOFM containing polyvinyl alcohol(PVA) on the viability of vitrified-thawed blastocysts was investigated in experiment 2. BSA added at 5 or 10mg/ml to a vitrification solution showed significantly higher(p < 0.05) developmental rate to expanded and hatching blastocysts than SFCS, but there was no significant difference in the developmental rate to hatched blastocysts after thawing. Supplementation of a culture medium with EGF and/or FGF significantly increased(p < 0.05) embryo development to expanded blastocysts compared with control but showed no beneficial effect on the development to hatching or hatched blastocysts. Coculture of thawed embryos with granulosa cells in a TCM 199 containing 10% fetal calf serum(FCS) showed the highest developmental rate to expanded, hatching and hatched blastocysts among the groups tested. In conclusion, supplementation of a vitrification solution with BSA at 5mg/ml and culture of thawed blastocysts in a medium containing EGF and/or FGF can improve in vitro viability of bovine blastocysts frozen by vitrification.

• Journal of Korean Clinical Nursing Research
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• v.14 no.3
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• pp.99-115
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• 2008

Purpose: The purpose for this study was to identify the present state of Clinical Nurses with Expanded Role (CNERs) and provide basic data to refine the roles of CNERs. In this study, CNREs refers to nurses who perform techniques traditionally done by doctors and use titles such as clinical nursing specialist, educator with consultation, research assistant, coordinator, physician assistant and special examiner. Method: This study was conducted from September 1 to November 12, 2007. Data from 684 nurses from 38 hospitals who responded to the questionnaire were analyzed with descriptive statistics using the SPSS 14.0 program. Results: The mean percentage of time spent was, for direct practice, 41.1%, for education and counseling, 22.8%, for consultation and coordination, 10.4%, for research, 6.8%, for administration, 8.9% and for other activities, 10.0%. The most frequently implemented CNERs, activities included consultation and education for patients and their families, counseling by telephone, history taking, physical examination, reading examination results, psychosocial assessment, managing treatment, input of prescriptions, and writing up patient records, Although not frequently performed, nurses in some fields carried out invasive procedures and prescribed medication and laboratory tests. Conclusion: Although the number of hospital CNERs are rapidly increasing, there is still confusion about the title and framework as well as standards. Furthermore, because some nurses are prescribing medication and laboratory tests as well as performing techniques not traditionally done by nurses, there is an absolute need for a legalized system and systematic education system for the safety of patients who are being cared by all CNERs.

• Clinical and Experimental Reproductive Medicine
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• v.42 no.3
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• pp.94-100
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• 2015

Objective: The goal of this study was to ascertain optimal assisted hatching (AH) method in frozen embryo transfer. We compared the effect of depending on whether mechanical or laser-AH was performed before or after the vitrification of embryo development rate and blastocyst cell numbers. Methods: In order to induce superovulation, pregnant mare's serum gonadotropin followed by human chorionic gonadotropin were injected into 4- to 5-week-old female mice. 2-cell embryos were then collected by flushing out the oviducts. The Expanded blastocysts were recovered after the collected embryos were incubated for 48 hours, and were then subjected to artificial shrinkage (AS) and cross-mechanical AH (cMAH) or quarter-laser zona thinning-AH (qLZT-AH) were carried out using the expanded blastocysts before or after vitrification. After 48 hours of incubation, followed by vitrification and thawing (V-T), and blastocysts were fluorescence stained and observed. Results: The rate of formation of hatched blastocysts after 24 and 72 hours of incubation was significantly higher in the AS/qLZT-AH/V-T group than in the other groups (p<0.05). The cell number of the inner cell mass was higher in AS/V-T/non-AH and AS/V-T/cMAH groups than those of others (p<0.05). In the control group, the number of trophectoderm and the total cell number were higher than in the AS-AH group (p<0.05). Conclusion: The above results suggest that AS and AH in vitrification of expanded blastocysts lead to the more efficient formation of hatched blastocysts in mice.

• Journal of the Korea Concrete Institute
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• v.14 no.2
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• pp.207-215
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• 2002

This study is focusing on mixing the foamed concrete incorporated by waste expanded polystyrene(W-EPS), investigating the physical properties and offering a proper quality control method to the field engineers. Two types of W-EPS (type A and type B) were studied. Type A (B) had globular (crushed) shape and diameter of 3-5 (1-2) mm. The results show that the flow was suddenly reduced with increasing mixing quantity of two types, but it satisfies KS F 4039 until 60 ％ of mixing rate. In general, the absorption rate was suddenly reduced with increased mixing quantity of two types especially, in type A. Apparent specific gravity was 0.36∼0.53 and reduced with increasing mixing quantify of type A. But it increased in case of type B. Compressive strength and heat conduction rate increased with mixing with W-EPS than non-mixing W-EPS but reduced with mixing too much W-EPS. Based ong the results, it is believed that mixing with W-EPS can improve the recycle of industrial wastes and produce the high quality foamed concrete.