• Journal of the Korean Institute of Navigation
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• v.23 no.1
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• pp.75-84
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• 1999

Although many studies on modelling of port competitive situation have been conducted, both theoretical frame and methodology are still very weak. In this study, therefore, a new algorithm called ESD (Extensional System Dynamics) for the evaluation of port competition was presented, and applied to simulate port systems in northeast asia. The detailed objectives of this paper are to develop Unit fort Model by using SD(System Dynamics) method; to develop Competitive Port Model by ESD method; to perform sensitivity analysis by altering parameters, and to propose port development strategies. For these the algorithm for the evaluation of part's competition was developed in two steps. Firstly, SD method was adopted to develop the Unit Port models, and secondly HFP(Hierarchical Fuzzy Process) method was introduced to expand previous SD method. The proposed models were then developed and applied to the five ports - Pusan, Kobe, Yokohama, Kaoshiung, Keelung - with real data on each ports, and several findings were derived. Firstly, the extraction of factors for Unit Port was accomplished by consultation of experts such as research worker, professor, research fellows related to harbor, and expert group, and finally, five factor groups - location, facility, service, cargo volumes, and port charge - were obtained. Secondly, system's structure consisting of feedback loop was found easily by location of representative and detailed factors on keyword network of STGB map. Using these keyword network, feedback loop was found. Thirdly, for the target year of 2003, the simulation for Pusan port revealed that liner's number would be increased from 829 ships to 1,450 ships and container cargo volumes increased from 4.56 million TEU to 7.74 million TEU. It also revealed that because of increased liners and container cargo volumes, length of berth should be expanded from 2,162m to 4,729m. This berth expansion was resulted in the decrease of congested ship's number from 97 to 11. It was also found that port's charge had a fluctuation. Results of simulation for Kobe, Yokohama, Kaoshiung, Keelung in northeast asia were also acquired. Finally, the inter port competition models developed by ESB method were used to simulate container cargo volumes for Pusan port. The results revealed that under competitive situation container cargo volume was smaller than non-competitive situation, which means Pusan port is lack of competitive power to other ports. Developed models in this study were then applied to estimate change of container cargo volumes in competitive relation by altering several parameters. And, the results were found to be very helpful for port mangers who are in charge of planning of port development.

• Journal of Korean Tunnelling and Underground Space Association
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• v.22 no.4
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• pp.451-468
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• 2020

Aging tunnels with small cross-sections can cause chronic traffic jams. This problem can be solved by widening the tunnel. In general, when the tunnel is expanded, the outer portion of the existing tunnel is excavated through a mechanical or blasting method. Such excavation affects not only the surrounding ground but also the existing tunnel. The application of the pre-cutting method can be a solution to these problems effectively. Therefore, if the widening of tunnel is performed by applying pre-cutting method, analysis of the impact of this method must be performed. In this study, in order to analyze the effect of applying pre-cutting in tunnel widening, numerical analysis is performed at six ground grades, from grade I to weathered rock. The analysis is performed with the expanding lane and the excavation length of pre-cutting as variables. In addition, the analysis is focused on the displacement of crown of the existing tunnel and the enlarged tunnel. As a result, the crown displacement of the enlarged tunnel is confirmed to converge at the same value regardless of the excavation length of the pre-cutting when the tunnel widening is completed. In the case of existing tunnels, uplift of crown occurs within 5 m of the front of the tunnel surface, and the shorter the excavation length of pre-cutting is found to be effective in preventing the occurrence of uplift.

• Journal of Korean Society for Geospatial Information Science
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• v.17 no.3
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• pp.71-79
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• 2009

We expanded the 3D building information extraction method using shadow and vertical line from single high resolution image with meta information into the method for single high resolution image without meta information. Our method guesses an azimuth angle and an elevation angle of the sensor and the sun using reference building, selected by user, on an image. For test, we used an IKONOS image and an image extracted from the Google Earth. We calculated the Root Mean Square (RMS) error of heights extracted by our method using the building height extracted from stereo IKONOS image as reference, and the RMS error from the IKONOS image and the Google Earth image was under than 3 m. We also calculated the RMS error of horizontality position by comparison between building position extracted from only the IKONOS image and it from 1:1,000 digital map, and the result was under than 3 m. This test results showed that the height pattern of building models by our method was similar with it by the method using meta information.

• Korean Journal of Construction Engineering and Management
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• v.15 no.4
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• pp.11-19
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• 2014

Construction VE activities has occupies an important position as a key tool of the project in terms of budget and performance improvements. Reflecting this situation, VE application scope is being expanded than before. Thus, construction projects are required the continued research and development for effective use of construction VE. VE target choice method is the central part in the construction VE work. But, this has a problem with low utilization in practical terms. Accordingly, this study was developed the new methodology(PVTCM) for VE target selection of the construction VE work. Its contents are as follows: First, to analyze practical problem for VE target choice method through VE practical data. Second, to extract the high potential VE object based on the cost model and ideas similarity by excellent VE cases analysis. Third, to implement standardized VE object item by using affinity diagram for each construction type items. This research were conducted to experts interviews for comparison analysis with the cost model as one of a representative target selection method. As a result, proposed method were implemented significantly higher performance in terms of practical effectiveness than conventional method.

• IMMUNE NETWORK
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• v.1 no.1
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• pp.87-94
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• 2001

Background: Cytokine-mediated ex vivo expansion has been proposed as a means of increasing the number of cord blood (CB) hematopoietic stem cells for transplantation. As well as stem cell number, stromal cells are necessary for functional maturation of hematopoiesis. The purpose of this study was to analyze the development of stromal cells during ex vivo expansion of CB $CD34^+$ cells. Methods : $CD34^+$ cells were purified from CB by magnetic bead selection. The levels of of interleukin-3, interleukin-$1{\beta}$, interleukin-6, granulocyte macrophagecolony stimulating factor and tumor necrosis factor-${\alpha}$ were measured in culture supernatants on 0, 1, 2, and 3 weeks, using ELISA techniques. CB $CD34^+$ cells were expanded in Iscoves modified Dulbeccos medium in the presence of several cytokines. The expression of E-selectin, vascular cell adhesion molecule-1, intercellular adhesion molecule-1, platelet/endothelial cell adhesion molecule-1, von Willebrand factor, vimentin, and CD14 in newly developed stromal cells was examined by immunocytochemical method. Relevant extracellular matrix (ECM) proteins and proper cytokines were also assayed for the most suitable condition for expansion of stromal cells. Results: Several cytokines were found to have been produced by CB $CD34^+$ cells as well as bone marrow-derived $CD34^+$ cells. During ex vivo expansion of CB $CD34^+$ cells, stromal cells appeared in the culture by day 4 and expanded over the following 7-10 days before being confluent by day 2 1. These cells expressed surface markers characteristic of cells of endothelial lineage. Furthermore, these stroaml cells also expanded effectively when treated with thrombopoietin+flt-3 ligand+stem cell factor+leukemia inhibitory factor or 0.1% poly-L-lysine-coated wells. Conclusion: Stromal cells were developed during ex vivo expansion of CB $CD34^+$ cells and that this development could be enhanced further by treating the stromal cells with cytokines or ECM.

• Journal of Embryo Transfer
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• v.21 no.3
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• pp.255-262
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• 2006

The purpose of this study is to investigate the effects of vitrification in open pulled straws (OPS) methods on in vitro survival ability of porcine embryos. For in vitro maturation of immature oocytes, the porcine ovaries were collected from local slaughter-house. The cumulus-oocytes complexes were aspirated from 2 to 6 mm follicles. The collected oocytes were cultured for in vitro maturation in NCSU-23 medium with 5 mM hypotaurine, 0.57 mM cysteine, 10% porcine follicle fluid, 10 IU/ml PMSG and 10 IU/ml hCG for $21{\sim}22$ hrs. Then, the oocytes were more cultured $21{\sim}22$ hrs in vitro maturation in medium removed hormones. The frozen-thawed spermatozoa were washed by centrifugation 2 times for 10 min at 1,500 rpm in D-PBS with 5.56 mM glucose, 0.33 mM Na-pyruvate, 100 IU/ml penicillin, $100 {\mu}g/ml$ streptomycin and 4 mg/ml BSA. The fertilization medium used mTBM with 2 mM caffeine and 2 mg/ml BSA and adjusted to a pH of 7.2 to 7.4. The final concentration of spermatozoa was adjusted to $2.5{\times}10^6$cells/ml motile sperm during fertilization in vitro. At 8 hrs after insemination, the oocytes were transferred into NCSU-23 medium with 5.0 mM hypotaurine, 4 mg/ml BSA and 10 ng/ml EGF and cultured for 7 days. When the blastocysts of different stages were frozen-thawed by OPS methods, the proportions of embryos with normal morphology were significantly (p<0.05) higher in embryos frozen-thawed at expanded blastocyst stage (38.9%) than in early blastocyst stage (28.3%). On the other hand, the proportions of embryos damaged after frozen-thawing were significantly (p<0.05) higher in embryos frozen at early blastocyst stages than in expanded blastocyst stage. In another experiment, the normal embryos morphology after frozen- thawing were further cultured for 48 hrs. After culture, the proportions of embryos hatched were 6.7, 20.0 and 33.3% for embryos frozen-thawed at early blastocyst, mid-blastocyst and expanded blastocyst stages. These finding indicate the possible broader application for OPS methods, as frozen-thawed embryos may be accompanied by developmental stage according to requirements of the survival ability after freezing of blastocyst stage in the pig.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.38 no.7
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• pp.647-656
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• 2014

To supplement the ISO-GUM method for the evaluation of measurement uncertainty, a simulation program using the Monte Carlo method (MCM) was developed, and the MCM and GUM methods were compared. The results are as follows: (1) Even under a non-normal probability distribution of the measurand, MCM provides an accurate coverage interval; (2) Even if a probability distribution that emerged from combining a few non-normal distributions looks as normal, there are cases in which the actual distribution is not normal and the non-normality can be determined by the probability distribution of the combined variance; and (3) If type-A standard uncertainties are involved in the evaluation of measurement uncertainty, GUM generally offers an under-valued coverage interval. However, this problem can be solved by the Bayesian evaluation of type-A standard uncertainty. In this case, the effective degree of freedom for the combined variance is not required in the evaluation of expanded uncertainty, and the appropriate coverage factor for 95% level of confidence was determined to be 1.96.

• Journal of the Korea Computer Industry Society
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• v.5 no.3
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• pp.393-404
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• 2004

Recently, public home have used a more than two computer connected with network, and several home appliances using independently with internet or network are developing to be related closely with the network. Therefore, the home utilized for a simple terminal of the global network in the past is being expanded to another part of the sub network. For a variety of connecting home-area protocols with the existing existing network, we require a new Residential Gateway(RG) that does not only make the home-area network operating in the sub network but also connects to the external network. In this paper, RG has intrinsic limits against flexible service due to IP address assignment and hardware capacity. In order to solve this problem in the RG, we propose a Management Server(MS). The MS that offers the integrated managements and control services for a variety of devices connected the RG in the home-area. It can not only solve the dynamic IP address assigning problem but also assigns private IP addresses to the home network devices through the Network Address Translation(NAT). It also provides somewhat useful functions for the home network and the RG for other additional services. <중략> The MS is using a SNMP protocol for managing the RG in the domain, a polling method of the MS and the RG compose a sequence polling method, a polling method using a multi-process and a multi-thread. In this paper, we introduce a problem with polling method separately, show a polling method between the MS and the RG using a multi-thread.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.3
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• pp.342-351
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• 2016

This study was carried out to develop an optimized analytical method using high-performance liquid chromatography (HPLC) applied to canthaxanthin, which is not yet designated as a food colorant in Korea, as well as to perform validation and uncertainty evaluation of this method. Official methods of AOAC, UK, and Japan with HPLC-UV detection were evaluated for the analysis of canthaxanthin by comparison of linearity, resolution, selectivity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision, recovery, inter-laboratory tests, and uncertainty measurement. The calibration curves showed high linearity with an $R_2$ value of over 0.999 for canthaxanthin standard solutions in all three official methods. The official method of Japan exhibited the best results in terms of resolution and selectivity, including the lowest LOD and LOQ. The average coefficients of variation were calculated as less than five of three institutes with a precision value less than 1, accuracy near 100%, and recovery ratio between $100{\pm}10%$. The expanded uncertainty for canthaxanthin was estimated to be $39.5{\pm}5.29mg/kg$ (95% confidence level, k=2), and the uncertainty of measurement was 13.4%. In this study, official methods of canthaxanthin were compared and the validities verified. The results will be further applied to establish an authorized analytical method for canthaxanthin in Korea.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.46 no.5
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• pp.139-145
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• 2009

Recently P2P (Peer-to-Peer) networks and applications have been expanded to various field. However, because the construction of P2P network did not influence a underlying network topology or property, it may be inefficient. Especially, this inefficiency is more serious in MANET because MANET has narrow bandwidth and energy constraint. When we use a proactive routing protocol in MANET, we can know hop counts between two nodes. And, we can construct a P2P overlay network using this hop count information. However, In case of using hop count only, we can avoid a interference path selection problem and a zig-zag problem. We propose a method of improving P2P contents distribution using virtual positions. We calculate virtual positions from hop counts using the trigonometry. This method solves a interference path selection problem and a zig-zag problem. This method also allows a P2P network to influence underlying network topology. We adopt this method to a network which uses a virtual geographic routing, a proactive routing protocol, or a reactive routing protocol. We show that our proposed methods improve P2P contents distribution performance over MANET through a simulation.