• Title/Summary/Keyword: ex-situ

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Effects of Phosphorous-doping on Electrochemical Performance and Surface Chemistry of Soft Carbon Electrodes

  • Kim, Min-Jeong;Yeon, Jin-Tak;Hong, Kijoo;Lee, Sang-Ick;Choi, Nam-Soon;Kim, Sung-Soo
    • Bulletin of the Korean Chemical Society
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    • v.34 no.7
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    • pp.2029-2035
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    • 2013
  • The impact of phosphorous (P)-doping on the electrochemical performance and surface chemistry of soft carbon is investigated by means of galvanostatic cycling and ex situ X-ray photoelectron spectroscopy (XPS). P-doping plays an important role in storing more Li ions and discernibly improves reversible capacity. However, the discharge capacity retention of P-doped soft carbon electrodes deteriorated at $60^{\circ}C$ compared to non-doped soft carbon. This poor capacity retention could be improved by vinylene carbonate (VC) participating in forming a protective interfacial chemistry on soft carbon. In addition, the effect of P-doping on exothermic thermal reactions of lithiated soft carbon with electrolyte solution is discussed on the basis of differential scanning calorimetry (DSC) results.

Towards Conservation of Threatened Ceropegia Species Endemic to a Biodiversity Hotspot: In Vitro Microtuber Production and Proliferation, a Novel Strategy

  • Pandit, Sagar Subhash;Nair, Aneeshkumar;Naik, Dhiraj Dilip
    • Journal of Forest and Environmental Science
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    • v.24 no.2
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    • pp.79-88
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    • 2008
  • Twenty-eight of 44 Indian Ceropegia species are endemic and their survival is threatened. As a step towards conservation, we implied in vitro methods for the sustainable propagule production in C. hirsuta, C. lawii, C. maccannii, C. oculata and C. sahyadrica. Effects of explant, growth regulators, sucrose and photoperiod were studied. High frequency microtuber production was achieved with the seedling-apical buds, grown on MS medium containing 4-6 mg $1^{-1}$ BAP, 3-8% (w/v) sucrose, under continuous illumination. Each microtuber, when subcultured proliferated to form a cluster of secondary microtubers. Every primary and secondary microtuber bore at least one shoot-bud and a root primordium. Each tuber (formed with any of the significantly effective treatments) weighed more than 500 mg, enough to plant directly in non-sterilized soils. Microtubers could be produced and proliferated round the year. Proliferation could be solely attributed to in vitro procedures as these plants bear solitary tubers in vivo. Microtubers could be sprouted in vitro to prepare ready to pot plantlets. As, this novel method succeeded for all five species, though they belong to different eco-physiological backgrounds, we recommend its implementation in the conservation programs for a broader range of Ceropegia species, supported by other integrated strategies.

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Determining the target strength bambood wrasse (Pseudolabrus japonicus) using Kirchhoff-ray mode

  • Kusdinar, Afriana;Hwang, Bo-Kyu;Shin, Hyeon-Ok
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.50 no.4
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    • pp.427-434
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    • 2014
  • Although ex situ target strength (TS) measurements using dual- and split-beam systems have become the primary approach of estimating fish abundance, theoretical model estimation is a powerful tool for verifying the measurements, as well as for providing values when making direct measurements is difficult. TS values for 20 samples of live bambooleaf wrasse (Pseudolabrus japonicus) whose target length (TL) ranged between 13.7 and 21.3 cm were estimated theoretically using the Kirchhoff-ray mode model, and the TS values for 18 live fish samples were additionally measured at ${\sim}0^{\circ}$ tilt angle to the swimming aspect using a tethered method at a frequency of 120 kHz to verify the theoretical values. The digitizing intervals used to extract the fish body and swim bladder morphology in the X-ray photographs significantly affected the calculated TS patterns, but variations based on the speed of sound and density ratio values for the general range of fish flesh were relatively small (within 1 dB). Close agreement was observed between the measured and theoretical TS values, and the correlation between the average TS and body length of the fish could be calculated accurately as <$TS_{120kHz}$>= 20logTL (cm) -71.6 using the theoretical method.

Trypanosoma brucei Infection in Asymptomatic Greater Kudus (Tragelaphus strepsiceros) on a Game Ranch in Zambia

  • Munang'andu, Hetron Mweemba;Siamudaala, Victor;Munyeme, Musso;Nambota, Andrew;Mutoloki, Stephen;Matandiko, Wigganson
    • Parasites, Hosts and Diseases
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    • v.48 no.1
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    • pp.67-69
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    • 2010
  • Trypomastogotes of Trypanosoma brucei were detected from 4 asymptomatic kudus (Tragelaphus strepsiceros) on a game ranch located approximately 45 km north east of Lusaka, Zambia. Blood smears examined from 14 wildlife species comprising of the impala (Aepyceros melampus), Kafue lechwe (kobus leche kafuensis), sable antelope (Hippotragus niger), tsessebe (Damaliscus lunatus), warthog (Phacochoerus aethiopicus), puku (Kobus vardoni), zebra (Equus burchelli), waterbuck (Kobus ellipsiprymnus), bushbuck (Tragelaphus scriptus), reedbuck (Redunca arundinum), wilderbeest (Connochaetes taurinus), hartebeest (Alcephelus lichtensteini), African buffalo (Syncerus caffer), and kudu (Tragelaphus strepsiceros) showed that only the kudu had T. brucei. Although game ranching has emerged to be a successful ex-situ conservation strategy aimed at saving the declining wildlife population in the National Parks, our findings suggest that it has the potential of aiding the re-distribution of animal diseases. Hence, there is a need for augmenting wildlife conservation with disease control strategies aimed at reducing the risk of disease transmission between wildlife and domestic animals.

The genetic structure of taro: a comparison of RAPD and isozyme markers

  • Sharma, Kamal;Mishra, Ajay Kumar;Misra, Raj Shekhar
    • Plant Biotechnology Reports
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    • v.2 no.3
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    • pp.191-198
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    • 2008
  • Germplasm characterization and evolutionary process in viable populations are important links between the conservation and utilization of plant genetic resources. Here, an investigation is made, based on molecular and biochemical techniques for assessing and exploiting the genetic variability in germplasm characterization of taro, which would be useful in plant breeding and ex situ conservation of taro plant genetic resources. Geographical differentiation and phylogenetic relationships of Indian taro, Colocasia esculenta (L.) Schott, were analyzed by random amplified polymorphic DNA (RAPD) and isozyme of seven enzyme systems with specific reference to the Muktakeshi accession, which has been to be proved resistant to taro leaf blight caused by P. colocasiae. The significant differentiations in Indian taro cultivars were clearly demonstrated by RAPD and isozyme analysis. RAPD markers showed higher values for genetic differentiation among taro cultivars and lower coefficient of variation than those obtained from isozymes. Genetic differentiation was evident in the taro accessions collected from different regions of India. It appears that when taro cultivation was introduced to a new area, only a small fraction of genetic variability in heterogeneous taro populations was transferred, possibly causing random differentiation among locally adapted taro populations. The selected primers will be useful for future genetic analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers identified in the DNA fingerprinting study will be useful for screening a segregating population, which is being generated in our laboratory aimed at developing a taro genetic linkage map.

Design of an Experience Monitoring and Sampling System for Context-aware Mobile Applications (상황 인지형 모바일 애플리케이션의 사용자 경험 모니터링 및 수집 시스템의 디자인)

  • Seo, Jung-Suk;Lee, Seung-Hwan;Kim, Ho-Jin;Lee, Gee-Hyuk
    • 한국HCI학회:학술대회논문집
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    • 2009.02a
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    • pp.834-840
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    • 2009
  • This paper discusses about an empirical study to design a suitable experience monitoring and sampling system for mobile phone applications. We designed a new experience sampling method (ESM) research system iteratively. The research system is characterized by context-aware experience sampling, real-time data management, supporting data analyzing tools, and integrating ESM with ex-situ diary. From literature review, we derived initial design of the ESM research system. With the first system, we held the first experiment that evaluates three applications. From the experiment, we found that more functions are required, thus we improved the ESM system. Finally, we propose an ESM system design goal for the third iteration with experience of two experiments.

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디젤오염토양의 과산화수소 처리시 중금속 용출 및 백연발생 특성

  • Jang, Na-Yeong;Jeong, Byeong-Gil;No, Gi-Hyeon;Go, Hyeon-Ung;Choe, Yeong-Ik;An, Yeong-Hui;Seong, Nak-Chang
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2008.11a
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    • pp.443-446
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    • 2008
  • 본 연구는 과산화수소를 이용하여 디젤오염토양의 처리시 중금속 용출특성, 백연가스 발생특성 등을 평가하기 위하여 실험을 하였다. 중금속 용출속도는 반응시간 15분까지 용출이 지속적으로 증가하다가, 15분 이후 점차적으로 감소하는 경향을 보였다. 이러한 과산화수소(H$_2$O$_2$) 처리방법은 토양내 중금속 용출속도를 가속화시켜 실제 현장에서 적용될 경우 이로 인하여 지하수에 심각한 영향을 미칠 수 있으므로 ex-situ 방법 등과 같은 방법을 고려한 2차 오염에 대한 대책이 필요할 것으로 판단된다. 또한, 백연가스의 발생량은 과산화수소의 농도가 높을수록 많이 발생하는 것으로 관찰되었으며, 이는 높은 농도의 과산화수소와 유류오염토양이 급격하게 반응하기 때문인 것으로 판단된다. 백연가스 중 Hexane이 가장 높은 농도로 검출되었으며, 이 외에도 Cyclopentane, Benzene, Ethylbenzene, m,p-Xylene, Undecane 등이 많이 발생하는 것으로 나타났다. 따라서 백연가스 중에 함유된 고농도의 휘발성유기화합물(VOCs)이 주변 환경에 유해한 영향을 미칠 것으로 예상되므로 적절한 대기오염방지시설을 설치하여 제거한 후 대기로 방출하는 것이 필요하다고 판단된다.

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Effect of Electrolytes on Electrochemical Properties of Magnesium Electrodes

  • Ha, Se-Young;Ryu, Anna;Cho, Woosuk;Woo, Sang-Gil;Kim, Jae-Hun;Lee, Kyu Tae;Kim, Jeom-Soo;Choi, Nam-Soon
    • Journal of Electrochemical Science and Technology
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    • v.3 no.4
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    • pp.159-164
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    • 2012
  • Magnesium (Mg) deposition and dissolution behaviors of 0.2 M $MgBu_2-(AlCl_2Et)_2$, 0.5 M $Mg(ClO_4)_2$, and 0.4M $(PhMgCl)_2-AlCl_3$-based electrolytes with and without tris(pentafluorophenyl) borane (TPFPB) are investigated by ex situ scanning electron microscopy (SEM) and galvanostatic cycling of Mg/copper (Cu) cells. To ascertain the factors responsible for the anodic stability of the electrolytes, linear sweep voltammogrametry (LSV) experiments for various electrolytes and solvents are conducted. The effects of TPFPB as an additive on the anodic stability of 0.4M ($(PhMgCl)_2-AlCl_3$/THF electrolyte are also discussed.

Rescue, rehabilitation and release of finless porpoise (Neophocaena asiaeorientalis) in Korea (한국 상괭이(Neophocaena asiaeorientalis)의 구조, 재활, 방류)

  • KIM, Moon-Jin;SOHN, Hawsun
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.3
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    • pp.861-871
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    • 2016
  • The coastal water of Korean Peninsula is the major habitat for finless porpoises. However, hundreds of finless porpoises are currently caught incidentally by stow-net fishery every year. To prevent the mass mortality of finless porpoises and conserve marine animals, Busan Aquarium has been designated as a "Marine Life Ex-Situ Conservation Institution and Marine Animal Conservation Center," and Cetacean Research Institute, under the National Institute of Fisheries Science have started RRR (rescue, rehabilitation and release) project together since 2011. From 2012 to 2013, four individuals of finless porpoises had been rescued and two of them finally released to their original habitat after fully recovered in July 2013. As a result, according to growth curve of finless porpoise of Korea more than 140 cm of body length, 38 kg of total weight referred to adult, results of observation of the change in the hormone revealed that mating time of finless porpoises is January, Jun and July. To become the initial institution which rescues, treatment, husbandry and releases finless porpoise in Korea.

Effects of Cryoprotectants and Diluents on the Cryopreservation of Spermatozoa from Far Eastern Catfish, Silurus asotus

  • Gil, Hyun Woo;Lee, Tae Ho;Park, In-Seok
    • Development and Reproduction
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    • v.21 no.1
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    • pp.79-91
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    • 2017
  • The aim of this study was to compare the efficacy of cryopreservation methods for ex situ conservation of spermatozoa from far eastern catfish, Silurus asotus. The spermatozoa activity index (SAI) and hatching rates were higher in spermatozoa stored in Alserver's solution than those of spermatozoa stored in glucose solution. The SAI and hatching rates in all experimental groups gradually decreased with increasing duration of storage. Additionally, the SAI and hatching rates gradually decreased with increasing thawing temperatures at all storage durations (P<0.05). Based on the SAI and hatching rates, our results suggest that the optimal cryopreservation conditions of catfish spermatozoa involve storage in Alserver's solution with 15% ethylene glycol, and thawing at $25^{\circ}C$. Cryopreservation of spermatozoa is a useful and reliable technique for conserving gene resources and for artificial propagation of far eastern catfish.