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Analysis of Chicken Feather Color Phenotypes Classified by K-Means Clustering using Reciprocal F2 Chicken Populations (K-Means Clustering으로 분류한 닭 깃털색 표현형의 분석)

  • Park, Jongho;Heo, Seonyeong;Kim, Minjun;Cho, Eunjin;Cha, Jihye;Jin, Daehyeok;Koh, Yeong Jun;Lee, Seung-Hwan;Lee, Jun Heon
    • Korean Journal of Poultry Science
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    • v.49 no.3
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    • pp.157-165
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    • 2022
  • Chickens are a species of vertebrate with varying colors. Various colors of chickens must be classified to find color-related genes. In the past, color scoring was performed based on human visual observation. Therefore, chicken colors have not been measured with precise standards. In order to solve this problem, a computer vision approach was used in this study. Image quantization based on k-means clustering for all pixels of RGB values can objectively distinguish inherited colors that are expressed in various ways. This study was also conducted to determine whether plumage color differences exist in the reciprocal cross lines between two breeds: black Yeonsan Ogye (YO) and White Leghorn (WL). Line B is a crossbred line between YO males and WL females while Line L is a reciprocal crossbred line between WL males and YO females. One male and ten females were selected for each F1 line, and full-sib mating was conducted to generate 883 F2 birds. The results indicate that the distribution of light and dark colors of k-means clustering converged to 7:3. Additionally, the color of Line B was lighter than that of Line L (P<0.01). This study suggests that the genes underlying plumage colors can be identified using quantification values from the computer vision approach described in this study.

Variation of Protein Content and Amino Acid Composition in Perilla Germplasm (들깨 유전자원의 단백질함량과 아미노산조성)

  • Lee, Jung-Il;Bang, Jin-Ki;Lee, Bong-Ho;Kim, Kwnag-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.35 no.5
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    • pp.449-463
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    • 1990
  • To obtain the basic informations on quality improvement, seed protein and amino acid composition were analyzed in 460 strains of perilla germplasm. Among the tested strains, total protein content ranged from 17.9% to 28.1 % with the 23.6% of varietal means. Form the experiment, Namji, Sandong, and Eunjin were selected as high protein strains of which content was as high as 28.1%. In protein content, collected strains from Jeonnam province showed highest, and was not significantly different by maturity, but this characteristics showed differences by seed coat color and 1,000 seed weight. The significantly negative correlation was observed between protein content and seed setting ratio. However it was observed that significant and high positive correlation between protein and oil content. A calibration for an Infra-Alyzer 450 using log reflectance readings at 2208, 1982, 1940 and 1722nm could be used without adjustment for the measurment of the protein content in perilla with a standard deviation of differences against micro-kjeldahl of 0.27%. The amino acid composition of perilla was similar to the other oilseed crops, and showed a relatively high lysine and methionine content. Further, amino acid composition of perilla seed was exellently characterized with bal ance and higher than FAO recommendation. Major amino acids were indentified as a glutamic acid and arginine in perilla seed protein.

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Analysis of the Ergothioneine Content in the Fruiting Bodies of Sawdust-cultivated Lentinula edodes Cultivars in Korea (한국의 표고 톱밥재배 품종의 에르고티오네인 함량 분석)

  • Min-Jun Kim;Yeun Sug Jeong;Eunjin Kim;Yeongseon Jang;Kang-Hyeon Ka
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.301-310
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    • 2022
  • Lentinula edodes is a type of mushroom widely consumed in East Asia, and many of the components contained in them are used as raw materials for pharmaceuticals or as health supplements. Ergothioneine, a sulfur-containing amino acid in L. edodes, is attracting attention due to its antioxidant and anti-inflammatory effects. L. edodes generally contain an ergothioneine content of about 200 to 800 mg per kg; there are some differences in this value depending on the cultivation conditions. In this study, 24 domestic sawdust-cultivated L. edodes cultivars were cultured under the same conditions, and the morphological characteristics and ergothioneine content of the fruiting bodies were investigated. The yield and morphological characteristics of the fruiting bodies were different in each cultivar. Taehyanggo and Sanjo 713ho had the largest and thickest pileus. The pileus of L. edodes had a higher ergothioneine content than the stipe. Sanjo 715ho had the highest ergothioneine content in the pileus and the stipe at 1,225 mg/kg and 753 mg/kg, respectively. An ergothioneine content of more than 1,000 mg/kg in the pileus was observed in Bambithyang, Sansanhyang, Sulbaekhyang, Taehyanggo, Sanjo 705ho, Sanjo 709ho, Sanjo 715ho, and Sanjo 716ho. These cultivars can be used as parental strains to develop new cultivars with enhanced ergothioneine functionalities.

Vertical Transmission of RNA Mycoviruses in Lentinula edodes (표고에서의 RNA 바이러스 수직감염)

  • Eunjin Kim;Mi-Jeong Park;Min-Jun Kim;Yeun Sug Jeong;Yeongseon Jang;Kang-Hyeon Ka
    • The Korean Journal of Mycology
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    • v.50 no.4
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    • pp.263-274
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    • 2022
  • Lentinula edodes is an important commercial mushroom and there have been several reports of viral infections in L. edodes. Two mycoviruses (LeV-HKB and LeNSRV1) were detected in Sanbaekhyang (NIFoS 2778) and Taehyanggo (NIFoS 4317), the sawdust-cultivated commercial strains. The vertical transmission rates of the viruses were investigated by detecting the viruses in 80 monokaryotic strains derived from basidiospores isolated from the fruiting bodies of each strain. Most of the monokaryotic strains were infected with the virus and the two viruses showed different levels of meiotic stability, with LeV-HKB showing higher meiotic stability than LeNSRV1. Therefore, it seems that the vertical transmission mechanism of mycoviruses is different depending on the virus species. We also examined the mycelial growth rate of the monokaryotic strains and compared the growth rate according to virus infection status. Although there was no statistically significant correlation between viral infection and mycelial growth rate, we found that the average growth rate was reduced by additional virus infection. We expect our data to contribute to a greater understanding of the mechanism of the vertical transmission of mycoviruses, and promoting breeding using virus-free monokaryotic strains.

Influence of Agricultural Water Return flow on Aquatic Ecosystem in Downstream (농업용수 회귀수량이 하천 수생태에 미치는 영향)

  • Lim, Eunjin;Kim, Jonggun;Shin, Yongchul;An, Hyunuk;Nam, Won Ho;Lim, Kyoung Jae;Lee, KwangYa
    • Proceedings of the Korea Water Resources Association Conference
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    • 2020.06a
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    • pp.246-246
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    • 2020
  • 최근 우리나라에서 농업용수의 다원적 기능에 대한 공감대가 형성되고 작물생육에 필요한 관개용수로만 인식되던 농업용수의 개념이 농촌생활환경개선을 포함하는 다양한 지역용수로의 포괄적 개념으로 전환되고 있다. 농업용수는 식량생산 이외의 효용을 위한 다원적 기능을 가지며 농촌지역의 각종 생산활동과 생활조건의 유지개선을 위한 농업용수의 다원적 기능에 관한 관심이 증가하는 추세이다. 농업용수에서 발생하는 회귀수는 유역의 용수공급계획, 하천 유황의 예측, 관개용수 사용량 결정, 하천 건전화 방지, 수생생태계 보호 및 생물 다양성 확보 등 농업용수의 효율적 사용 및 환경생태유지를 위해 매우 중요한 역할을 하고 있다. 농업용수 회귀수량은 농업용수 중 하천으로 회귀하는 수량을 의미한다. 본류의 생태 유량 확보에 농업용수 회귀 수량이 기여하고 있으며 본류 하천의 환경 보전 기능을 하고 있다. 또한, 수생태계 보호 및 생물 다양성 확보 등 환경 생태 유지에 매우 중요한 역할을 하고 있다. 하지만 농업용수 회귀수량이 하천 수생태에 미치는 영향 분석 연구는 미흡한 실정이다. 따라서 본 연구의 목적은 대사 저수지 유역을 대상으로 농업용수 회귀 수량이 하류 하천 수생태에 미치는 영향을 정량적으로 평가하고자 한다. 본 연구에서는 회귀 수량은 관개용수량, 배수량, 침투량, 담수심 등 물수지 항목을 논물수지 모형에 적용하여 산정하였으며, 하류 하천의 생태유량 산정을 위해 대사저수지 하류에 위치한 생물측정망 자료를 통해 대표 어종을 선정하였다. PHABSIM 모형을 이용해 모의 대상 지역 특성 자료 및 인근 소하천 생물측정망 자료를 바탕으로 HSI 기반 대표 어종 서식처 환경에서 최적 생태유량을 산정하였다. 이를 통해 추정된 농업용수 회귀 수량에 따른 필요 유량이 어류 서식환경에 미치는 영향을 평가하였다. 본 연구 결과 대사저수지 하류 하천에서 농업용수 회귀수량이 차지하고 있는 기여도 큰 것으로 분석되었으며, 최적 생태유량과 비교한 결과 농업용수 회귀수량이 수생태(어류)에 미치는 영향이 큰 것으로 나타났다. 따라서 농업용수 회귀수량은 하류 하천의 하천유지수량뿐만 아니라 하천 환경생태유지에도 매우 중요한 역할을 하고 있음을 알 수 있다.

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Gender Differences in Contribution of Antisocial Personality and Affective Empathy to Psychopathy (대학생들의 정신병질에 대한 반사회성과 정서적 공감의 영향: 성차를 중심으로)

  • Kim, Beomjun;Lee, Eunjin;Kim, Youngyoun
    • The Korean Journal of Woman Psychology
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    • v.14 no.2
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    • pp.219-239
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    • 2009
  • This study investigated gender differences in contribution of antisocial personality and affective empathy to psychopathy. Two hundred fifteen college students (male, 118; female,97) completed three measures including the PPI-R(Psychopathic Personality Inventory-Revised), the PAI-ANT(Personality Assessment Inventory-Antisocial Features), and the EETS (Emotional Empathy Tendency Scale). According to the results, the mean scores of PPI-R and EETS in male group were higher than in female group. Antisocial features were positively associated with psychopathy in both male and female groups and negatively with affective empathy. Males showed that the scores of affective empathy were negatively associated with those of antisocial features and psychopath. Females showed that the scores of affective empathy were negatively associated with those of antisocial personality and psychopathy but the correlation was significant only with the scores of antisocial behavior (ANT-A). To scrutinize the differences between male and female groups, the scores on the PAI and EETS were compared between 30% upper and 30% lower groups based on the scores of the PPI-R. According to the results, the upper group of the scores on the PPI had higher scores on the PAI and lower scores on the EETS. The differences of the scores on the EETS between high-psychopathy and low-Psychopathy groups were bigger in male group than in female group. Explanations and implications of the results were discussed and the directions of the future studies were suggested.

The relation among narcissistic personality, depression, and psychopathy: Focus on gender differences (대학생의 자기애, 우울과 정신병질 성향과의 관계: 성차를 중심으로)

  • Kim, Young Youn;Lee, Eunjin;Kim, Beom Jun
    • The Korean Journal of Woman Psychology
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    • v.16 no.1
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    • pp.97-113
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    • 2011
  • The current study was conducted to explore the relations among college students' narcissistic personality, depression, and psychopathy. This study investigated how narcissistic personality and depression variables relate to psychopathy variables. For this purpose, an assessment battery consisting of Narcissistic Personality Inventory(NPI), Narcissistic Personality Disorder Scale: NPDS), BDI, Self-Reported Psychopathy Scale(SRPS), and Psychopathic Personality Inventory-Revised. PPI-R was administered to 1003 college students (501 males and 502 females). For comparison with the levels of pathological narcissism, the participants were devided two groups( above 33%, below 33% ) based on their scores of the NPDS, According to the results, males had more overt narcissistic personality, and females felt more depressive and psychopathic. The higher level group on the NPDS had higher scores on the NPI, BDI, SRPS and PPI-R. The students' narcissistic personality had relations to depression and psychopathy, but the results were different according to the kind of inventories(PPI-R or SRPS, NPI or NPDS). The implications and limitations of this study, as well as some suggestions for future research, were suggested.

A Study on Factors Related to Men's Thinking and attitudes on Prostitution and Gender: Based on the Previous Experience of Buying Sex (성인 남성의 성구매 경험에 따른 성매매와 성 관련변인에 대한 연구)

  • Eunjin Lee
    • Korean Journal of Culture and Social Issue
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    • v.15 no.3
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    • pp.377-398
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    • 2009
  • The purpose of this study was to examine the differences in men's thinking and attitude on prostitution and gender based on the previous experience of buying sex. It was an exploratory survey for planning future studies and movements for changing a way of thinking in relation to prostitution. A total of 1328 male adults(above 19 years) completed a questionnaire consisting of general thinking of buying sex, tolerance limit of sexual behavior, and sex role egalitarianism. The respondents answered differently in the probability of paying for sex in future and the way how to get a sex information according to the previous experience of buying sex. Those respondents who had paid for sex, had higher scores on three types of sex attitude measures than those who did not. The previous experience of buying sex was significantly correlated with sub-factors of buying sex, tolerance limit of sexual behavior, and sex role egalitarianism except for the sub-factor on recognizing a sex worker as a victim (factor 4 of buying sex). These results suggest that men who had paid for sex had more traditional gender-stereotypes and attitudes. Implications and limitations of this study were discussed in a feminist perspective.

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A Study on the Evaluation of the Adsorption Efficiency of Heavy Metals by the Content of Jellyfish Extract at Immunity Reaction in Alginate bead (알긴산 비드에 혼합된 해파리 면역 반응물질 함량에 따른 중금속 흡착효율 평가에 관한 연구)

  • Jong Hwan Kim;Hyeok Jin Park;Inho Choi;Eunjin Kim;I Song Choi;Jong-Min Oh
    • Journal of Environmental Impact Assessment
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    • v.32 no.6
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    • pp.431-436
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    • 2023
  • As the industry develops, the amount of heavy metals flowing into the ecosystem is increasing. Heavy metals are difficult to decompose and remain in the ecosystem for a long time and cause toxicity, which is removed by physicochemical methods such as adsorption, filtration, and chemical precipitation during water treatment. In this study, Alginate bead was selected as a chelating resin for adsorbing and removing heavy metals, and the Jellyfish Extract at Immunity reaction (JEI) were mixed to evaluate the adsorption efficiency of heavy metals accordingly. beads mixed with JEI showed high adsorption efficiency in lead (79-99%) and copper (64-70%) according to the characteristics of Alginate, and low adsorption efficiency in cadmium (25-37%) and zinc (5-6%). Although heavy metal adsorption did not increase in proportion to the content of JEI, 50% and 100% JEI beads showed significant increases. As a result of applying the reaction rate equation, it was found that it was more suitable for the pseudo-secondary reaction equation than the pseudo-first reaction equation.

Characteristics of Lentinula edodes Fruiting Bodies Formed by Mono-mono Hybridization Using Mating Type Analysis (교배형 분석을 활용한 표고 교배균주 육성 및 자실체 발생 특성)

  • Yeongseon Jang;Mi-Jeong Park;Eunjin Kim;Min-Jun Kim;Yeun Sug Jeong;Kang-Hyeon Ka
    • The Korean Journal of Mycology
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    • v.51 no.4
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    • pp.389-396
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    • 2023
  • In this study, dikaryotic strains of Lentinula edodes were generated by mono-mono hybridization using mating type analysis and their fruiting body characteristics were investigated. Approximately 100 monokaryotic strains were isolated from the basidiospores of Sanbaekhyhang, and homokaryotic strains were isolated from Chungheung 1ho using protoplast isolation and regeneration. Their mating types were evaluated and a total of 60 dikaryotic strains were hybridized. Using these strains, fruiting bodies were produced and their characteristics were examined after cultivation on sawdust media. The results indicated that the rate of hybridization was 100% and that 55 of 60 strains formed fruiting bodies. These showed normal pileus and gill structures; however,10 strains also produced fruiting bodies with abnormal pileus and gill structures. The weight and size of the fruiting bodies differed depending on the strains. Overall, further studies are needed for predicting the characteristics of hybridized strains based on their parental strains.