• Korean Journal of Acupuncture
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• 제38권1호
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• pp.32-42
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• 2021

Objectives : In this study, we investigated the neuroprotective effects of ethanol extract of Lonicera japonica flower buds (EELJ) on glutamate-induced neurotoxicity in mouse hippocampus-derived neuronal HT22 cells. Methods : After analyzing the cytoprotective effect of EELJ on glutamate in HT22 cells, the inhibitory effect of apoptosis was studied using flow cytometry. In order to analyze the antioxidant efficacy of EELJ, the levels of reactive oxygen species (ROS) and glutathione (GSH) were investigated, and the effects on the activities of superoxide dismutase (SOD) and catalase (CAT) were also analyzed. Furthermore, the effect of EELJ on the expression of apoptosis regulators such as Bax and Bcl-2 in glutamate-treated HT22 cells was investigated. Results : According the current results, pretreatment with EELJ significantly reduced glutamate-induced loss of cell viability and release of lactate dehydrogenase. EELJ also markedly attenuated glutamate-induced generation of intracellular ROS, which was associated with increased levels of GSH, and activity of SOD and CAT in glutamate-stimulated HT22 cells. In addition, EELJ was strikingly inhibited glutamate-induced apoptosis in HT22 cells. Furthermore, the expression of pro-apoptotic Bax was increased and the expression of anti-apoptotic Bcl-2 was decreased in glutamate-treated HT22 cells, while in the presence of EELJ, their expressions were maintained at the control levels. Conclusions : These findings indicate that EELJ protects glutamate-induced cytotoxicity in HT22 hippocampal neurons through antioxidant activity. Therefore, although identification of biologically active substances of EELJ and re-evaluation through animal experiments is necessary, this natural substance is a promising candidate for further research in preventing and treating oxidative stress-mediated neurodegenerative diseases.

• 생약학회지
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• 제53권4호
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• pp.213-225
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• 2022

Schisandra chinensis is widely known to regulate fatigue recovery, lowering blood pressure, and contains several bioactive compounds such as schizadran. In this study, we obtained basic data for the development of health food by measuring the schizandrin content, antioxidant activity, anti-inflammatory, antibacterial activity, and enzyme activity of the hot water extract (SCW) and 40% ethanol extract (SCE) of S. chinensis grown in Sunchang-gun, Korea. Schizandrin content was measured as 7.87 ± 0.01 mg/g for SCW and 10.38 ± 0.05 mg/g for SCE. SCE had higher DPPH and ABTS radical scavenging activity than SCW, and the total polyphenol and flavonoid contents were also higher. Both SCW and SCE (500 ㎍/mL) exhibited more than 55% protective effects against oxidative stress in HepG2 cell lines. Anti-inflammatory efficacy was assessed using RAW 264.7 and Caco-2 cells and both SCW and SCE do not have cytotoxic effects. Anti-inflammatory results showed concentration-dependent NO activity and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) inhibition. The antibacterial activity increased with increasing dose concentrations of SCW and SCE, and the MIC was 25 mg/mL for L. monocytogenes, S. typhimurium, and 75 mg/mL for H. pylori. In addition, amylase and protease enzyme activity was observed in both SCW and SCE.

• 대한본초학회지
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• 제34권3호
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• pp.1-7
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• 2019

Objectives : Amyloid ${\beta}(A{\beta})$ could induce cognitive deficits through oxidative stress, inflammation, and neuron death in Alzheimer's disease (AD). This study was investigated the effect of Angelica keiskei KOIDZUMI (AK) on memory in $A{\beta}$-induced an AD model. Methods : AK was extracted uses 70% ethanol solvent. Total polyphenol and flavonoids content were obtained by the Folin-Ciocalteu and the Ethylene glycol colorimetric methods, respectively. The antioxidant activities were assessed through free radical scavenging assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) methods. Intracerebroventrical (i.c.v) injection of $A{\beta}$ 1-42 was used to induce AD in male ICR mice, followed by administrations of 5, 10 or 20 mg/kg AK on a daily. Animals were subjected to short and long term memory behavior in Y-maze and passive avoidance test. Results : The total polyphenol and flavonoids contents of the AK extract were $88.73{\pm}6.36mg$ gallic acid equivalent/g, $84.21{\pm}5.04mg$ rutin equivalent/g, respectively. The assays of DPPH and ABTS revealed that AK extract in treated concentrations (31.25, 62.5, 125, 250, 500, $1000{\mu}g/m{\ell}$) increased antioxidant activity in a dose-dependent manner. Oral administration of AK extract significantly reversed the $A{\beta}$ 1-42-induced decreasing of the spontaneous alternation in the Y-maze test and $A{\beta}$ 1-42-induced shorting of the step-through latency in the passive avoidance test. Conclusions : The findings suggest that AK indicated the antioxidant protective effects against $A{\beta}$-induced memory deficits, and therefore a potential lead natural therapeutic drug or agent for AD.

• 대한본초학회지
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• 제34권1호
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• pp.99-107
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• 2019

Objectives : Cudrania tricuspidata (C. tricuspidata) is well-known traditional herbal remedy and its root, leaf and fruit were used for treatment of inflammation, tumor and painkilling. However, effect of C. tricuspidata fruit on tight junction is still unknown. The aim of this research was to determine the effect of C. tricuspidata fruit extract on human keratinocyte HaCaT cells. Methods : The antioxidant effects of water extract of C. tricuspidata (WECT) and ethanol extract of C. tricuspidata (EECT) were analyzed by using an ABTS assay. To confirm the cytotoxicity of WECT and EECT, MTS assay was performed. The mRNA expression levels of tight junction related genes were analyzed using quantitative RT-PCR analysis. Furthermore, dispase assay was used to investigate the alteration of cell-cell adhesion strength of EECT treated HaCaT cells. Results : WECT and EECT showed strong antioxidant activity. No obvious cytotoxicity was observed in both WECT and EECT until $2.0mg/m{\ell}$ concentration. The mRNA expression level of Claudin 6 were significantly increased by EECT treatment, whereas the WECT did not affect the expression of Claudin 6. Furthermore, EECT treatment enhances cell-cell adhesion strength. Conclusions : In this study, we investigated the physiological activities of the extracts of Cudrania tricuspidata fruit extracts on human keratinocytes by two different extraction methods. EECT might have an anti-aging activity on the skin by reducing oxidative stress. Moreover, it may be a useful ingredient in atopic dermatitis and skin-moisturizing, given its effects of altering Claudin 6 gene expression and enhancing cell-cell adhesion strength.

• 대한본초학회지
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• 제24권3호
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• pp.103-108
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• 2009

Objectives : This study was carried out to investigate anti-oxidative effects of Taraxaci Herba (TH) and protective effects on Human HaCaT keratinocyte. Methods : Anti-oxidative effects were measured by estimating the amount of total phenolics and flavonoids. In addition, DPPH free radical scavenging activities were estimated. Protective effects of TH on HaCaT keratinocytes against oxidative stress induced by hydrogen peroxide were also measured. Results : In our results, treatment with TH did not show cytotoxicity on HaCaT keratinocyte beneath the concentration of 200 $\mu$g/ml. 42.64$\pm$1.90 $\mu$g/ml of total phenolics and 28.09$\pm$1.84 $\mu$g/ml of flavonoids was detected from TH ethanol extract. In addition, DPPH free radical scavenging activities of TH were elevated in dose-dependent manner. In addition, The value of half maximal inhibitory concentration (IC$_{50}$) was 165.5 $\mu$g/ml. Finally, TH showed protective effect against cell death of HaCaT cell induced by hydrogen peroxide significantly. Conclusions : In conclusion, these results suggest that TH may have anti-oxidantic action in human skin and also suggest the possibility as cosmetic material.

• 한국안전학회지
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• 제39권1호
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• pp.9-15
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• 2024

Hydrogen-based electricity and transportation systems are widely recognized as sustainable power sources. However, the low ignition energy of hydrogen, only 1/10th that of conventional fossil fuels, poses a safety concern involving the risk of ignition due to electrostatic discharge from facility workers. Therefore, anti-static systems are imperative for hydrogen-based electricity facilities. To address this, we propose a reliable conductive rubber mat (CRM) to ensure the safety of these facilities. Unlike conventional anti-static floors that utilize conductive paint (CP), the CRM features a uniform distribution of conductive components in chemically and mechanically stable rubber. As a result, the CRM is unyielding to polar solvents (such as ethanol and hydrosulfuric acid) and non-polar solvents (like mineral oil) without increasing its resistance. Moreover, the CRM can withstand mechanical stress. Consequently, the human-body voltage of workers on the CRM would be sufficiently low enough to protect them from hydrogen explosions, thereby enhancing overall safety.

• 한국식품과학회지
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• 제48권3호
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• pp.284-288
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• 2016

본 연구에서는 고포도당 조건에서 로젤의 산화방지 효과와 산화 손상에 대한 신경세포 보호효과를 검증하였다. 총 페놀 함량은 아세트산에틸 분획물(71.41 mg GAE/g)이 다른 분획물보다 높았으며, 이에 따라 아세트산에틸 분획물로 in vitro 산화방지 실험을 진행한 결과 상대적으로 우수한 ABTS 라디칼 소거활성과 지방질과산화물 생성 억제효과를 나타내었다. 신경세포에 고포도당으로 유발시킨 산화 스트레스에 대한 로젤 아세트산에틸 분획물은 산화 스트레스의 생성을 억제하고 신경세포 생존율을 향상 시켰다. 더불어 신경전달물질인 아세틸콜린 분해하는 아세틸콜린 에스터레이스의 억제효과를 보였으며, 마지막으로 HPLC 분석 결과 로젤 아세트산에틸 분획물에 존재하는 주요 페놀화합물은 클로로겐산인 것으로 판단되었다. 결국 클로로겐산을 함유하는 로젤 아세트산에틸 분획물은 우수한 산화방지 효과와 신경세포 보호효과 그리고 아세틸콜린 에스터레이스 억제 효과를 유도한 것으로 추정되며, 이를 통해 고포도당 상태에서 발생하는 당뇨성 퇴행성 신경질환과 같은 질병을 예방할 수 있는 고부가가치 소재로서의 활용가치가 있다고 판단된다.

• 대한화장품학회지
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• 제38권3호
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• pp.255-261
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• 2012

피부는 끊임없이 외부환경에 노출되며 가장 주요한 스트레스 요인 중 하나는 자외선방사이다. 자외선 방사로 인하여 피부에 염증, 착색, 광노화 및 피부암을 포함하는 생물학적 영향을 끼치게 된다. 본 연구에서는 해양천연소재 (김, 다시마, 모려, 모자반, 미역, 석결명, 우뭇가사리, 청각, 톳, 파래)로부터 피부보호 항산화제를 조사하기 위해 자외선으로부터 기인된 세포보호 효과를 증명하였으며 세포독성, 산화에 의한 세포사멸, 항노화 효과에 대하여 평가를 실시하였다. 그 결과, 모자반, 한천, 석결명, 청각 에탄올 추출물을 처리한 군이 자외선으로 유도된 세포독성 및 세포사멸을 효과적으로 억제하는 것으로 나타났다. 또한 다른 실험에서 자외선에서 인한 세포사멸은 세포내 축적이나 ROS로부터 매개되지만 해양추출물을 처리함으로써 현저하게 감소되었다. 이러한 해양추출물의 보호효과 증가는 Type I collagen과 Type I procollagen에 의해 매개하는 것과 연관되는 것으로 사료된다. 이들의 결과는 해양추출물이 노화예방 및 항산화제로서의 우수한 특성으로 피부손상에 의한 산화적 스트레스에 대응하는 새로운 기능성 소재로서의 가능성을 가지는 것으로 평가된다.

• 생명과학회지
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• 제13권1호
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• pp.110-117
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• 2003

고온내성을 가진 효모 균주, Saccharomyces cerevisiae KNU5377을 황산, 질산 그리고 염산에 노출시켰다. 스트레스 원으로써 무기산은 물에서 쉽게 해리되어 외부 산도를 떨어뜨린다. 여러 가지 무기산이 첨가된 조건에서 배양한 결과 KNU5377은 0.4%의 황산, 질산 농도에서 생육이 가능한 반면 대조 균주인 S. cerevisiae ATCC24858은 이 보다 낮은 농도인 0.3%가 생육의 한계였다. 더욱이 KNU5377은 0.35%의 염산에서 90분 이상, 0.6%의 황산에서는 30분 이상 생존이 가능한 높은 내성을 나타내었다. 반면에 두 균주 모두 0.45%의 질산에서는 생존하지 못하였다. 0.3%의 황산이 첨가된 조건에서 알코올 발효 시 KNU5377은 이틀 후 3%의 알코올을 생산하였다. 더욱이 0.2%의 황산 첨가와 동시에 $40^{\circ}C$ 고온에서도 4.5%의 높은 알코올 생산이 관찰되었다. 또한 황산 0.2%에 한 시간동안 노출시킨 뒤 세포내에 축적되는 trehalose의 농도를 측정한 결과, KNU5377에서는 30분내에 효과적으로 축적되었으며 동일한 스트레스 조건에서 전자현미경(TEM)을 통한 세포의 형태의 관찰 시 어떠한 변화도 나타나지 않았다.

• 한국식품저장유통학회지
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• 제12권5호
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• pp.482-488
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• 2005

산화적인 스트레스(Oxidative stress)는 신경염증의 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 약용곤충추출물을 이용하여 항산화 물질을 찾고자 초고속 적용가능한 스크리닝 방법을 적용하였다. 우선, 분자염증은 활성산소 관련의 물질과 밀접한 관련이 있으므로 이들의 억제를 동반하는 추출물을 먼저 선별하였다. 항산화 실험과 관련하여 DPPH (1,1-Diphenyl-2-picrylhydrazyl), FRAP(Ferric ion reducing antioxidant power), HO (Hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 assay하였고 hydrogen peroxide(H2O2)에 의한 세포사멸 억제 활성을 보기 위해 MTT assay를 실시하였다. 실험 결과, 사마귀, 늦반딧불이, 무당벌레에서 다른 library에 비교하여 항산화 활성이 높게 나타났다.