• The Korean Journal of Food And Nutrition
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• v.6 no.2
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• pp.109-114
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• 1993

This study was divised to observe an Inhibitory effect toward a lipolytic action of toxohormone-L from large root and small root Nepal pseudo ginseng (NPG ; Nepal products) components by water extract and ethanol precipitate in vitro. Toxohormone-L is known to be a lipolytic factor that was partially purified from the ascites fluid of Sarcoma 180-bearing mice and of patients with hepatoma. The inhibitory effect that inhibited the lipolytic action of toxohormone-L by ethanol Precipitate component of large root NPG (mean 55.5%) was higher (mean 1.37 times) than that of water extract component in final reaction concentration of 500 and 1, 000ug/ml, on the other side inhibitory effect of water extract component in small root NPG (mean 55.5%) was higer (mean 1.14 times) than that of ethanol precipitate component. In a way inhibitory effect of precipitate component In large root NPG(47.6%), when final reaction concentration of sample were 1, 000ug/ml, was about 40% lower than that of Korean red ginseng.

• The Korean Journal of Food And Nutrition
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• v.4 no.1
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• pp.75-80
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• 1991

This study was divised to observe an inhibitory toward a lipolytic action of toxohormone-L from large root and small root Nepal pseudo ginseng(NPG ; Nepal products) components by water extract and ethanol precipitate in vitro. Toxohormone-L Is known to be a lipolytic factor that was partially purified from the ascites fluid of sarcoma 180-hearing mice and of patients with hepatoma. The inhibitory effect that inhibited the lipolytic action of toxohormone-L by ethanol precipitate component of large root NPG(mean 46.8%) was higher (mean 1.8 times) than that of water extract component in final reaction concentration ,5001g1m1, on the other side inhibitory effect of water extract component in small root NPG(mean 43.9%) was higher(mean 1. 2 times) than that of ethano1 precipitate component, respectively. In a way inhibitory effect of ethanol precipitate component in large root NPG(47.6%), when final reaction concentration of sample were 1,000 U g/ml, was about 4095 lower than that of Korean red ginseng, respectively.

• Journal of Ginseng Research
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• v.4 no.1
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• pp.72-87
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• 1980

Red ginseng tails were extracted with ethanol solutions over a range of concentrations and temperature conditions. Investigations were carried out to study the effects of treatments on yields, soluble solids, saponin and precipitate occured in red ginseng extract beverage during storage. It was found that: (1) Higher concentration of ethanol at low temperature resulted in less yield of crude extract (2) The amount of precipitate in the non-purified extract beverage were less with decrease in ethanol concentration used (3) The treatment for purification of extracts and storage of purified extract at 37$^{\circ}C$ for 6 months had no effect on HPLC chromatogram pattern of saponins (4) The amount of purified extract decreased by purification treatment and more decrease was found as the temperature and concentration of ethanol increased. For Preparation of red ginseng extract beverage, the treatment of extracts with ethanol at low temperature was found to be more effective to minimize precipitation in tile beverage.

• Journal of Life Science
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• v.23 no.2
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• pp.282-289
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• 2013

This study was conducted to investigate the effect of Makgeolli and Makgeolli precipitate on hepatotoxity and the serum lipid content in rats. First, we investigated the effect of Makgeolli and ethanol on the progress of alcoholic fatty liver. The effect of Makgeolli precipitate on carbon tetrachloride ($CCl_4$)-induced hepatotoxicity in the rats was then studied. Indicators of the health status of the experimental period, the body weight gain in ethanol-treated group tended to be lower than those in the control and the Makgeolli-treated groups. The weight of the liver tissue decreased significantly following the administration of ethanol. However, this was not seen following the administration of Makgeolli. The activities of serum aspartate transaminase (AST) and alanine transaminase (ALT) were decreased in the Makgeolli group compared to the ethanol group. Serum cholesterol concentrations increased in the ethanol group, but decreased in the Makgeolli-treated group to an equal volume of the ethanol-treated group. The serum HDL-cholesterol content was significantly higher in the Makgeolli group than in the ethanol group. Analysis of the impact of the Makgeolli precipitate on toxicity induced by $CCl_4$ in the liver showed that the $CCl_4$ treatment significantly increased the activities of serum ALT and AST. However, the levels of cholesterol and triglyceride in serum were decreased. The $CCl_4$ treatment increased the activities of AST and ALT. However, the raw Makgeolli precipitate decreased their activities. Moreover, raw Makgeolli precipitate significantly reduced the $CCl_4$-induced elevation of serum lipids more than heated Makgeolli precipitate. These results suggest that raw Makgeolli precipitate may exert a protective effect against $CCl_4$-induced liver injury by preventing lipid peroxidation.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.4
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• pp.601-604
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• 2006

The objective of this study was to investigate technical methods for extraction of mucopolysachharide-protein containing chondroitin sulfate from keel cartilage of chickens. The chemical composition of chicken keel cartilage was determined. For the preparation of mucopolysaccharide-protein from lyophilized chicken keel cartilage, hot water extraction and alcalase hydrolysis methods were examined. Results showed that the optimum condition of hot water extraction was incubation for 120 min with a yield of 40.09% and chondroitin sulfate content of 28.46%. For alcalase hydrolysis, the most effective condition was 2% alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate was 75.87%, and chondroitin sulfate content was 26.61%. For further separation of chondroitin sulfate from the alcalase hydrolysate, which has a higher yield than that of hot water, 60% ethanol precipitation was performed. The yield of the ethanol precipitate was 21.41% and its chondroitin sulfate content was 46.31%. The hot water extract, alcalase hydrolysate and ethanol precipitate showed similar electrophoretic migration with standard chondroitin sulfate (chondroitin sulfate A), using cellulose acetate membrane electrophoresis. These results indicated that a significant amount of mucopolysaccharide-protein containing chondroitin sulfate could be acquired form chicken keel cartilage. Therefore, keel cartilage in chicken may provide an inexpensive source of chondroitin sulfate for commercial purposes.

• Korean Journal of Materials Research
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• v.8 no.2
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• pp.126-130
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• 1998

Aluminum sulfate, $AI_{2}(SO_4)_3\cdot18H_2O$ was prepared by adding of kaolin-dissolved sulphuric acid into ethanol and its thermal decomposition behavior was discussed. As-synthesized high purity precipitate particles were platelike shapes of $2\mu\textrm{m}$ size. With increasing drop rate of leach liquor into ethanol, the crystallite sizes of the precipitate decreased. The apparent activation energies for dehydration and sulfate decomposition of the precipitate were $11.9Kcal mol^{-1}$ and $48.2kcal mol^{-1}$ respectively.

• Journal of Dairy Science and Biotechnology
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• v.33 no.2
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• pp.153-157
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• 2015

Milk mineral, which is also called milk calcium, was recovered from concentrated skim milk ultrafiltration permeate (CUFP). Lactose, the major constituent of CUFP, was crystallized by the addition of ethanol; lactose precipitation was observed to increase as the ratio of CUFP to ethanol increased. The calcium content of CUFP remained constant at a CUFP to ethanol ratio of 1:2, while it significantly decreased at a CUFP to ethanol ratio of 1:4. When ethanol (95%, v/v) was reused to precipitate lactose out of CUFP, 85% of the initial lactose precipitated out, while 82% of calcium remained soluble in the CUFP after storage for 24 h.

• Korean Journal of Food Science and Technology
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• v.32 no.3
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• pp.618-628
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• 2000

The indigestible dextrin I was prepared by hydrolyzing pyrodextrin with thermostable ${\alpha}-amylase$. The mean values of indigestible fraction and dieatry fiber of indigestible dextrin I prepared from yellow dextrin were 50.0% and 25.0%, respectively. Also the indigestible dextrin II was prepared by removing low molecular weight saccharides containing glucose with ethanol from enzyme hydrolysate of pyrodextrins. Over 80% of glucose and maltose in initial enzyme hydrolysate were removed, therefore the indigestible fraction and dietary fiber of the indigestible dextrins increased. The indigestible dextrin from ethanol precipitate of enzyme hydrolysate of yellow dextrin by ${\alpha}-amylase$ and amyloglucosidase showed a higher contents of indigestible fraction and dietary fiber than ethanol precipitates by any other enzyme combination, and its mean values were 83.6% and 62.8%, respectively. Consequently, it was found that the indigestible dextrins which are resistant to starch-hydrolysing enzyme can be easily prepared from pyrodextrin, and presumed that they can perform physiological functions as soluble dietary fiber.

• Bulletin of the Korean Chemical Society
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• v.20 no.4
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• pp.431-435
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• 1999

An organic precipitate flotation of Cd(Ⅱ) and Pb(Ⅱ) was investigated by the coprecipitation with lanthanum 8-hydroxyquinolinate. Trace amounts of Cd(Ⅱ) and Pb(Ⅱ) with a significantly large amount of La(Ⅲ) were simultaneously precipitated in a 1,000 mL sample solution with the ethanolic 8-hydroxyquinoline solution. The pH was adjusted to 9.0 with 2 M ammonia solution. The precipitates were floated with the aid of tiny nitrogen bubbles and supported by the stable foam layer of sodium lauryl sulfate. The floats were collected on the fritted glass filter by a suction. The material collected was dissolved with 5.0 mL of ethanol and 1.5 mL of concentrated nitric acid, and then diluted to 25.0 mL with a deionized water. The analytes were determined by a flame atomic absorption spectrophotometry. The recoveries of the analytes spiked in the sample were 94.8% for Pb(Ⅱ) and 92.0% for Cd(Ⅱ). This flotation technique is simple and rapid, and also applicable to the determination of trace Cd(Ⅱ) and Pb(Ⅱ) at lew ppb levels.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.26-27
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• 2004

This study was conducted to evaluate the value of chicken keel cartilage as a source of mucopoly-saccharide-protein containing chondroitin sulfate (CS) and to find the optimum extraction conditions. The hot water extraction and alcalase hydrolysis methods were performed for extraction mucopolysaccharide in lyophilized chicken keel cartilage. The most efficient condition was hydrolysis with 2 % alcalase in 10 volumes of distilled water for 120 min. The yield of hydrolysate and CS content were 75.87 % and 25.61 %, respectively. For further separation of CS from hydrolysate by alcalase, ethanol precipitation was performed. The yield of ethanol precipitate and its CS content were 21.41 % and 46.31 %, respectively.