• Korean Journal of Medicinal Crop Science
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• v.6 no.2
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• pp.114-120
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• 1998

The present study was carried out to investigate the changes in essential oil contents as a part of searching the changes in active components in the graft cultivation of A. senticosus. To increase the yield of aerial parts and the contents of active components of Acanthopanax senticosus, scions of A. senticosus were grafted on different rootstocks. As a result, the contents of calarene, ${\alpha}-beramotene$ and spathulenol increased, but cis-caryophyllene and epizonarene contents decreased. ${\beta}-pinene$, ${\beta}-myrcene$, 2,5,5 -trimethyl-1, 3, 6-heptatriene and ${\beta}-elemene$ were not detected in the leaves of grafted A. senticosus. Essential oils from stems did not differ with the methods of cultivation. Oil contents of 3-year-old plants were higher than those of 1-year-old plants, whereas ${\alpha}-bergamotene$ content(27%) was highest in the stem of grafted A. senticosus(27%). Ethylbenzene, 2-furancarboxaldehyde, M134 $(t_R=2.11)$ and M205 $(t_R=3.16)$ disappeared in the roots of A. senticosus var. subinermis, but 6,6-dimethyl-3-methylenebicycloheptane, M166 $(t_R=1.82)$, and (+) -aromadendrene and ${\beta}-bisabolene$ were newly found.

• International Journal of Oral Biology
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• v.42 no.1
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• pp.25-31
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• 2017

Dental caries is the most common chronic disease in the dental field. Streptococcus mutans (S. mutans) is the most important bacteria in the formation of dental plaque and dental caries. In a previous study, we confirmed that the essential oil of Chrysanthemum boreale has antibacterial activity against S. mutans. Alpha-pinene is one of the major chemical components of Chrysanthemum boreale essential oil. In the present study, we investigated the inhibitory effects of ${\alpha}-pinene$ on cariogenic properties such as growth, acid production, biofilm formation, and bactericidal activity on S. mutans. Alpha-pinene at a concentration range of 0.25-0.5 mg/mL significantly inhibited the growth of S. mutans and acid production of S. mutans. Biofilm formation was significantly inhibited at > 0.0625 mg/mL ${\alpha}-pinene$, similar to the data from scanning electronic microscopy. Under confocal laser scanning microscopy, the bacterial viability was decreased by ${\alpha}-pinene$ in a dose-dependent manner. These results suggested that ${\alpha}-pinene$ may be a useful agent for inhibiting the cariogenic properties of S. mutans.

• Journal of Microbiology and Biotechnology
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• v.17 no.9
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• pp.1568-1572
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• 2007

To develop a natural fungicide against Botrytis cinerea and Colletotrichum gloeosporioides, a total of 25 essential oils were tested for their fumigant activity against post-harvest pathogens. The vaporous phases of oils were treated to each fungus on potato dextrose agar medium in half-plate separated Petri plates at $10\;{\mu}g$ per plate. The essential oil of Illicium verum strongly inhibited the mycelial growth of both B. cinerea and C. gloeosporioides by over 90%. On the other hand, the essential oil of Schizonepeta tenuifolia showed inhibitory activity against mycelial growth of only B. cinerea by over 90%. Gas chromatography-mass spectrometry and bioassay indicated trans-anethole in I. verum and menthone in S. tenuifolia as a major antifungal constituent. The essential oils of I. verum and S. tenuifolia and their major constituents could be used to manage post-harvest diseases caused by B. cinerea and C. gloeosporioides.

• Applied Biological Chemistry
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• v.45 no.2
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• pp.101-107
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• 2002

The essential oils isolated from leaves, flowers, stems, and fruits of Vitex rotundifolia by steam distillation and extraction (SDE) method were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). A total of 76 components detected by GC, 42 components were identified positively by GC-MS and GC co-injection with authentic standards, and 34 components were identified tentatively by mass spectral data only. They included 16 monoterpene hydrocarbons, 30 oxygenated hydrocarbons, 10 sesquiterpene hydrocarbons, 8 oxygenated sesquiterpenes, 3 diterpenes, and 9 miscellaneous components. The major components in the oil from the leaves were ${\alpha}-pinene$ (30.25%), 1,8-cineole (19.89%), sabinene (9.56%), ${\alpha}-terpineol$ (7.94%), ${\beta}-pinene$ (5.69%), and terpinen-4-ol (2.37%), and those in the flower oil were ${\alpha}-pinene$ (25.47%), 1,8-cineole (7.69%), manoyl oxide (6.21%), ${\beta}-pinene$ (4.20%), ${\alpha}-te.pineol$ (3.76%), and sabinene (2.78%). The major components in the oil from the stems were ${\alpha}-pinene$ (13.24%), ${\alpha}-terpineol$ (10.64%), 1,8-cineole (4.40%), manoyl oxide (4.02%), ${\beta}-pinene$ (2.39%), and terpinen-4-ol (2.21%) while those in the oil from the fruits were ${\alpha}-pinene$ (20.24%), 1,8-cineole (11.47%), ${\beta}P-pinene$ (9.79%), ${\alpha}-terpineol$ (7.08%), sabinene (3.68%), and limonene (2.77%). The percentage composition of monoterpenes in the oils from the leaves and the fruits were higher than in those from the flowers and the stems, whereas the oil from the flowers and the stems were characterized by a large content of sesquiterpenes, diterpenes and other unknown high molecular weight components.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09b
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• pp.745-746
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• 2006

This lecture introduces new press, adapter and control concepts to size multi-level sintered components. The essential thing here is that the advantages of the multi-plate technology have been applied to the sizing adapter concept. Thus, the new concept meets the demands for a modern P/M manufacture and offers sufficient potential to size any future, complex sintered components such as synchronizer hubs, oil pump wheels and VCT parts with highest precision. Furthermore, it outlines a new flexible concept for the parts transfer, including feeding, orientation and lubrication while responding to the high demands on process stability and short change-over times.

• Proceedings of the PSK Conference
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• 2001.04a
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• pp.197.3-198
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• 2001

• Proceedings of the Korean Society of Crop Science Conference
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• 1992.05a
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• pp.134-135
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• 1992

• YAKHAK HOEJI
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• v.37 no.1
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• pp.54-65
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• 1993

A method for the determination of 16 residual pesticides in crude deugs was examined. Crude drug was extracted with acetonitrile/water solution and the extract was partitioned between hexane/CH$_{2}$Cl$_{2}$ and NaCl saturated water. The organic layer was passed through cleanup column and subjected to the GC/ECD or GC/NPD analysis. Essential oil components in crude drug interfere with the analysis, and sulfuric acid treatment was adapted to overcome this interference.

• Korean Journal of Medicinal Crop Science
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• v.10 no.3
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• pp.206-211
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• 2002

For developing natural presevatives, essential oils of Mentha arvensis L. var. piperascens Malivaud and Agastache rugosa O. Kuntze were analyzed the composition of two oils and experimented on microorganism survival. Main components of Mentha arvensis oil were isomenthol (26.84%) and menthol (25.48%), and those of Agastache rugosa oil were estragole (79.83%) and limonene (4.13%) from GC-MSD analysis. Inhibition activities of Mentha arvensis oil against growth of Escherichia coli O157 : H7 ATCC 43895 and Salmonella typhimurium ATCC 7988 were observed from their clear zone $(9{\sim}14 mm\;&\;9{\sim}13\;mm)$, and that of Agastache rugosa oil were done from the clear zone $(13{\sim}20 mm\;&\;10{\sim}18\;mm)$ by concentration-dependent manner, respectively. In the inhibition test on CFU/ml of the microorganisms, both of the plant essential oils at concentration of 5 and 10 mg showed potent growth inhibition activities from 9 hour of incubation. Analysis using transmission electron microscope on E. coli also showed antimicrobial activities of the oils as deformation of the cell and loss of the intracellular materials.

• Korean Journal of Medicinal Crop Science
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• v.3 no.3
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• pp.217-225
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• 1995

In this study the essential oil and pharmacologically active components of different valerian species (Valeriana fauriei var. dasycarpa Hara and V. officinalis L. from Korea and V. wallichii DC from Nepal) were analyzed to search for native plant resources that can be used for medicinal herb and tobacco flavoring. The oil contents in dry roots of V. fauriei, V. officinalis and V. walhchii were 1. 71%, 0. 82% and 0. 83 %, respectively. The composition of the essential oil among different valerian species was compared. In V. fauriei 47 compounds were identified, while 21 and 25 compounds were identified in V. officinalis and V. wallichii. The major compounds in the oil of V. fauriei were bornyl acetate (33.8%) and camphene (10.8%), cedrol (4. 1 %), -pinene (3. 2%) and unidentified sesquiterpene alcohol (3. 0%). The major compounds were borneol (62. 5%) and ${\beta}-sesquiphelandrene$(6. 8%) and spathulenol (2. 1%) in V. officinalis, and borneol (74. 3%) and ${\alpha}-elemene$ (2.7%) and -sesquiphellandrene (2. 3%) in V. wallichii. Among the components known as major pharmacologically active compounds in European or Japanese valerian roots, valeranone, valerenal and -kessyl acetate was detected in a small amount in V. fauriei, but kessoglycol diacetate was not detected in V. faudei and V. officinalis Among the valepotrate compounds, major pharmacologically active components in V. wallichii, valtrate was detected in a small amount in V. fauriei and V. officinalis., and detected 1. 42% in V. wallichii. Didrovaltrate was also detected in the three valerian species tested, but acevaltrate was not detected except V. wallichii. On the other hand, antioxidative activity was slightly higher in V. fauriei than those of V. officinalis.