• Korean Chemical Engineering Research
• /
• 제54권1호
• /
• pp.70-74
• /
• 2016

본 연구에서는 창자파래(Enteromorpha intestinalis)로부터 citrate buffer를 사용하여 전처리 조건(고액비, 반응온도, buffer의 pH와 농도)에 따른 전처리 반응과 효소가수분해를 통한 가수분해 수율을 조사하였다. 0.25 M, pH 3.5의 citrate buffer를 이용하여 $140^{\circ}C$에서 60분간 전처리를 수행한 결과, 5.40%의 가수분해 수율을 얻었다. 최종적으로 전처리 반응 후 24시간의 효소 가수분해를 통하여 18.68%의 가수분해 수율을 얻었다. 이 결과는 대조구에 비하여 약 1.81배 증가한 결과이다.

• KSBB Journal
• /
• 제30권6호
• /
• pp.332-338
• /
• 2015

The objective of this study was designed to determine the possibility of bioethanol production from wasted medium density fiberboard (wMDF). We were investigated the enzymatic saccharification characteristics using the enzyme (Cellic CTec3) after pretreatment with sodium chlorite. According to the component analysis results, the lignin contents before and after the pretreatment of wMDF (milling using sieve size of $1,000{\mu}m$) was significantly reduced from 31.13% to 4.11%. Therefore, delignification ratio of pretreated wMDF was found to be up to about 87-89% depending on the sieve size. And we were tested to compare the saccharification ratio according to the sieve size of wMDF ($1,000{\mu}m$, $200{\mu}m$), but it was no significance depending on the sieve size. When enzyme dosage was 5% based on the substrate concentration, enzymatic saccharification ratio was obtained up to 70% by maintaining at $50^{\circ}C$ for 72 hours. We could made the substrate concentration of pretreated wMDF ($1,000{\mu}m$) up to 12% and then enzymatic saccharification ratio was 76.8%, also contents of glucose and xylose were analyzed to 77,750 and 14,637 mg/L, respectively.

• Journal of the Korean Wood Science and Technology
• /
• 제40권4호
• /
• pp.294-301
• /
• 2012

본 연구는 농업 부산물인 옥수숫대를 이용하여 옥살산 전처리와 효소가수분해를 통한 에탄올 발효 효율 향상조건을 탐색하였다. 옥살산 전처리 옥수숫대의 효소가수분해는 Accellerase 1000을 이용하였으며, $50^{\circ}C$ 온도조건과 pH 4.5에서 96시간 가수분해하여 가장 높은 단당류 수율인 $64.8g/{\ell}$의 단당류 수율을 나타냈다. 옥수숫대에서 생산된 단당류의 발효에는 Pichia stipitis CBS 6054를 공시균주로 사용하였고, 전처리 옥수숫대 및 효소 투입량이 각각 10~14%와 15 FPU 이었을 때 효율적인 에탄올 생산에 가장 적합한 것으로 판명되었다. 이러한 조건에서 24시간 발효 후에 약 88.2%의 에탄올 전환율에 해당되는 0.45의 에탄올 수율을 얻었다.

• 에너지공학
• /
• 제24권3호
• /
• pp.1-5
• /
• 2015

본 연구는 바이오에탄올을 생산하고자 목질계 바이오매스의 효소당화에서의 반탄화의 영향을 비교분석하였다. 전처리로서, 목질계 바이오매스의 반탄화는 무산소 조건에서 $250{\sim}350^{\circ}C$의 온도로 시행되었다. 또한 비이온성 계면활성제인 Tween-80을 첨가하여 반탄화로 인한 소수성변환에 대처하여 당화효율을 높이기 위한 실험을 진행하였다. 그 결과, 반탄화 전처리한 바이오매스를 효소당화한 후 글루코즈 생산량이 전처리하지 않은 바이오매스의 글루코즈 생산량보다 높았다. 그리고 Tween-80의 첨가하여 효소당화하였을 때 당 전환율이 더 높았다. 이로 인해 반탄화를 목질계 바이오매스의 전처리로 적용할 수 있으며 Tween-80을 첨가하였을 때 효소당화에 영향이 있다는 것을 알 수 있었다.

• 한국식품과학회지
• /
• 제50권2호
• /
• pp.203-208
• /
• 2018

본 연구에서는 항염증 펩타이드를 생산하기 위해 도루묵(Arctoscopus japonicus)의 어육과 알로부터 가수분해물을 제조하였으며, 단백분해효소, pH, 온도, 효소 농도 및 가수분해 시간에 따른 NO 소거활성을 측정함으로써 최적 가수분해 조건을 설정하였다. 어육 가수분해물의 최적 조건은 pH 5.0, 온도 $30^{\circ}C$, 효소 농도 1%, 가수분해 시간 4시간으로 확인되었으며, 알 가수분해물의 최적 조건은 pH 5.0, 온도 $70^{\circ}C$, 효소 농도 3%, 가수분해 시간 3시간으로 확인되었다. 이러한 최적의 가수분해 조건에서 어육 및 알 가수분해물의 NO 소거활성은 각각 18.94 및 19.81%로 측정되었다. 이는 도루묵 단백질 가수분해물 유래 항염증 펩타이드를 생산하기 위한 기초자료로 활용할 수 있을 것으로 기대된다.

• ALGAE
• /
• 제18권4호
• /
• pp.341-347
• /
• 2003

The potential antioxidative activity of water-soluble enzymatic hydrolyzates from a kelp, Ecklonia cava was evaluated by free radical scavenging and lipid peroxidation assays. To prepare water-soluble hydrolyzates from E. cava the seaweed was enzymatically hydrolyzed by five carbohydrases (Viscozyme, Celluclast, AMG, Termamyl and Ultraflo) and five proteases (Protamex, Kojizyme, Neutrase, Flavourzyme and Alcalase). Among all the hydrolyzates, Celluclast hydrolyzate effectively scavenged free radicals released from DPPH (1,1-diphenyl-2- pricrylhydrazyl) and recorded around 73% scavenging activity at the concentration of 4 mg ${\cdot}ml^{-1}$. This hydrolyzate was thermally stable and DPPH radical scavenging activity remained 80% or higher at heating temperatures of 40 and 60$^{\circ}C$ up to 12 h and around 80% at 100$^{\circ}C$ up to 8 h. AMG and Ultraflo hydrolyzate inhibited the lipid peroxidation of fish oil as that of $\alpha$-tocopherol. These results suggested that an enzymatic extraction will be an effective way for the production of a potential antioxidant from seaweeds.

• 펄프종이기술
• /
• 제46권3호
• /
• pp.44-49
• /
• 2014

The mushroom cultured waste(MCW) from cork oak was evaluated as the raw material for bioethanol production. For enzymatic hydrolysis, cellulase cocktails (Celluclast 1.5L and Novozym 188) was used for polysaccharides to monosaccharides conversion. Compared with sound cork oak woodmeal, woodmeal from MCW showed higher cellulose to glucose conversion. To improve polysaccharides to monosaccharides conversion, pretreatment by sodium hydroxide was applied. Even though more xylan and lignin were removed in woodmeal of MCW than that of cork oak, concentration of glucose was higher from sodium hydroxide treated cork oak woodmeal (51.3 g/L) than treated MCW woodmeal (41.6 g/L).

• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 1978년도 추계학술대회
• /
• pp.206.4-206
• /
• 1978

By utilizillg whole cell enzyme of the Xantho-monas citri IFO 3835, cephalexin is synthesized directly from 7-amino-deacetoxy cephalosporanic acid (7-ADCA) and phenyl glycine methyl ester (PGM). To date, cephalexin has been manufactu-red by chemical process involving fairly large number of steps to protect the amino group of phenly glycine and carboxyl group of 7-ADCA. However, the enzymatic process involves only a single step with 85％ conversion in 90 minutes. The fermentation variables studied indicate that oxygen transfer is limiting step in the enzyme production. Optimum conditions for enzymatic reaction were 37 C, pH 6.0, and the optimum substrate molar ratio of PGM to 7-ADCA was 2. Other variables that are related to the biochemical properties of whole cell enzyme temperature stability, pH stability, kinetic constants, reusing effect, enzyme loading effect were also evaluated.

• 한국신재생에너지학회:학술대회논문집
• /
• 한국신재생에너지학회 2005년도 제17회 워크샵 및 추계학술대회
• /
• pp.688-688
• /
• 2005

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제10권4호
• /
• pp.346-352
• /
• 2005

The chemical characteristics, enzymatic saccharification, and ethanol fermentation of autohydrolyzed lignocellulosic material that was exposed to steam explosion were investigated using bagasse as the sample. The effects of the steam explosion on the change in pH, organic acids production, degrees of polymerization and crystallinity of the cellulose component, and the amount of extractive components in the autohydrolyzated bagasse were examined. The steam explosion decreased the degree of polymerzation up to about 700 but increased the degree of crystallinity and the micelle width of the cellulose component in the bagasse. The steam explosion, at a pressure of 2.55 MPa for 3 mins, was the most effective for the delignification of bagasse. 40 g/L of glucose and 20 g/L of xylose were produced from 100 g/L of the autohydrolyzed bagasse by the enzymatic saccharification using mixed cellulases, acucelase and meicelase. The maximum ethanol concentration, 20 g/L, was obtained from the enzymatic hydrolyzate of 100 g/L of the autohydrolyzed bagasse by the ethanol fermentation using Pichia stipitis CBS 5773; the ethanol yield from sugars was 0.33 g/g sugars.