• 제목/요약/키워드: environmental identity

검색결과 388건 처리시간 0.027초

Isolation and Molecular Analysis of Methanol Oxidation Genes in an Obligate Methylotrophic Bacterium, Metheylobacillus sp. Strain SK-5

  • Choi, Hack-Sun;Kim, Jin-Kwon;Ahn, Yeong-Hee;Koh, Moon-Joo;Kim, Si-Wouk
    • Journal of Microbiology and Biotechnology
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    • 제12권5호
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    • pp.819-825
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    • 2002
  • Methanol dehydrogenase (MDH) is a key enzyme in the process of methanol oxidation in methylotrophic bacteria. However, information on MDH genes from genus Methylobacillus is limited. In this study, a 6.5-kb HindIII DNA fragment of Methylobacillus sp. SK-5 chromosomal DNA was isolated from the genomic library of the strain by using a degenerate oligonucleotide probe that was designed based on JV-terminal amino acid sequence of the MDH $\alpha$ subunit purified from the strain. Molecular analysis of the fragment revealed four tightly clustered genes (mxaFJGI) involved in the methanol oxidation. The first and fourth genes were very similar to mxaF (77% identity for nucleotides an 78% identity for amino acids) and mxaF (67% Identity for nucleotides and 68% Identity for amino acids) genes, respectively, from Methylovorus sp. SSI. Genes mxaF and mxaI encode $\alpha$ and $\beta$ subunits of MDH, respectively. The two subunits were identified from purified MDH from Methylobacillus sp. SK-5. A dendrogram constructed by comparison of amino acid sequences of MDH u subunits suggests that MxaF from Methylobacillus sp. SK-5 belongs to a subfamily cluster of MDH u subunits from $\beta$-subgroup Proteobacteria. The subfamily cluster is separated from the other subfamily that consists of $\beta$- and $\gamma$-subgroup Proteobacteria. This study provided information on mn genes from a methylotrophic bacterium in $\beta$-subgroup Proteobacteria, which would aid to better develop a gene probe to detect one-carbon metabolizing bacteria.

대구광역시 담장허물기운동의 정체성과 나아갈 방향 (The Identity and Some Practical Suggestions of The Demolishing Walls Campaign in Daegu City)

  • 김수봉;정응호;이승지
    • 한국주거학회논문집
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    • 제17권3호
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    • pp.51-60
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    • 2006
  • Ten years have passed since the Demolishing Walls Campaign has started in Daegu. Recently, the campaign influenced to the other cities in Korea, such as Seoul, Incheon and Busan with various names regarding cities' circumstances. In these context, the study aims to highlight the identity of the Demolishing Walls Campaign compare Daegu with other cities where similar movement has started. In addition, the study evaluates the campaign in terms of physical, psychological and economical aspects to suggest some practical suggestion for the development of the campaign based on questionnaire survey. The research findings are as follows. The research shows that the identity of the Daegu Campaign is started by bottom-up approach based on citizens' participation compare with others cities' campaigns which are mainly started by the local government so called top-down method. The Daegu campaign influences the local people aware of their potential abilities in positively improving urban environment. The results of survey also highlights the advantages of green spaces which by the campaign in terms of environmental and economical aspects. Newly generating urban greens accept and store heat which Influences urban microclimate conditions and it possibly affects energy savings of the city. Most of survey participants eager to continue this campaign which shows positive results in various aspects by the local people. Therefore, the city government building a coalition of local people, NGOs, and interest group to keep the identity of the campaign in Daegu.

도시 복합용도시설에 나타난 '지역성'의 특성에 관한 연구 - 일본 복합용도시설을 중심으로 - (A Study on the Locality in the Mixed-use Facilities - Focused on the Mixed-use Facilities in Japan -)

  • 원선영;박정아;이효창;하미경
    • 한국실내디자인학회:학술대회논문집
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    • 한국실내디자인학회 2008년도 춘계학술발표대회 논문집
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    • pp.285-289
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    • 2008
  • There has been a project in Japan recently to develop the mixed-use facility in order to help regenerate its city's vitality. They must make efficient use of resources that contains historical and local identity. In contrast, Korea hasn't considered developing their mixed-use facilities for maintaining their historical identity. This research will analyze how locality is reflected in the 8 mixed-use facilities in Japan. The research method was as follows: Preparatory study and literature research were conducted to extract the elements of locality shown in the mixed-use facilities in Japan. According to the results, the peculiarities of locality are as follows: 1) It's important to make the most of the local Identity by reflecting on the historical peculiarity of the region. 2) Nature and its environmental surroundings should be considered when constructing buildings in order to revitalize its local identity. 3) In the perception aspect, when a mixed-use facility is open for visit, the negative elements such as the 'peripheral regional gap' that may be felt by the visitor must be kept to the minimum.

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Exploring Factors on Identity of Korean Diaspora: Perspectives of Millennial Generation

  • HONG, Minoak;CHO, Yooncheong
    • 산경연구논집
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    • 제12권4호
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    • pp.15-26
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    • 2021
  • Purpose: The purpose of this study is to explore the major factors affecting the development of national identity of the Millennial Korean diasporas in the CIS countries that have rarely been explored in previous studies. In particular, this study examines how perceived identities have changed due to social, cultural, and other environmental changes and suggests policy considerations accordingly. Research design, data, and methodology: This study collected data via online survey. Factor and regression analyses were applied for data analysis. Results: The findings of this study suggest a set of factors that is different from the factors generally known to affect the diasporic identities of diasporas. The results of this study provide policy implications to help them construct identities that could more positively define their diasporic lives and relationship with homeland. Conclusions: The factors of direct experiences, such as relationship with host societies and homeland experience, exhibited strong relationship with national identity and life satisfaction of the Millennial Korean diasporas in the CIS countries. The unique characteristics of the Millennials and the long history of separation from homeland showed different results. The results of this study suggest policy considerations in regard to the Millennial diasporas in the CIS countries.

이야기를 통한 치유: 린다 호건의 『세상을 지켜보는 여자: 한 원주민의 회고록』 (Healing through Storytelling: Linda Hogan's The Woman Who Watches Over the World)

  • 전세재
    • 영미문화
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    • 제18권1호
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    • pp.1-21
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    • 2018
  • In Woman Watches over the World, Linda Hogan explores the broken identity of herself and her family, the issue of the poverty and the identity crisis, the alcoholism, prevalent in the Native American community and their silenced history. Previous studies have claimed that her memoir contributes to the restoration of Native American identity and history by accusing the violence of white culture, and seeks to recognize a dialogue between native culture and white mainstream culture as well. However they seem to overlook the complicated relations among story, identity, body and nature, to which Hogan as a multi-binded storyteller resorts as a way to break the silence of herself and her tribe for healing. Her own story, as a way to break the silence, becomes the formative drive to reveal the silenced history of her own tribe to lead the young generation to the future. She also understands the formative function of the story, which becomes the vehicle for embodying and connecting themselves to nature. To her, healing lies in the restoration of sympathetic relationship with nature. History, as a type of story, can be made up or mistold just like a story. There may be a blind spot where one can not assess what is true. In spite of the vision of the parallel worlds of the two cultures she presents, there seems to be no immediate solution to the discrimination against the Native American, poverty, identity crisis, and environmental problems which the Native American community faces. However, it can be said that her memoir serves as a rudder by presenting a direction to not only the Native American but also to readers in other cultures in its quest for practical possibilities for the future.

역사문화지구의 퍼블릭 아트에서 나타나는 표현 특성과 아이덴티티 연구 - 징더전 시 도계천을 중심으로 - (A Study on the Characteristics and Identity of Public Art in Historical and Cultural Areas - Focused on Taoxichuan in Jingdezhen -)

  • 이지엔화;윤지영
    • 한국콘텐츠학회논문지
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    • 제20권2호
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    • pp.82-95
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    • 2020
  • 본 연구는 역사문화지구 퍼블릭 아트의 특징과 아이덴티티를 연구 대상으로 역사문화구역의 퍼블릭 아트에 대한 이론 연구와 조사를 통해 징더전 도계천이 대표하는 역사문화지구 퍼블릭 아트의 특성을 연구하였다. 공간적 특성과 아이덴티티를 형성하는 여섯 가지 평가요인인 환경성, 공공성, 상징성, 예술성, 역사성, 상호작용성을 중심으로 도계천 퍼블릭 아트의 특성과 아이덴티티를 분석과 평가하였다. 연구 결과 산업 유산물로 재탄생 한 7개 큰 굴뚝의 퍼블릭 아트는 완전히 보존된 산업역사 건물과 어울린다. 그중, 환경성, 공공성, 상징성, 예술성, 역사성이 명확한 구현을 갖추고 있으며 단지의 역사적 상징물로서 보존가치와 식별가능성이 높아 일반 퍼블릭 아트의 행사나 체험기능을 더하지 않아도 된다. 지역 재생을 위한 신퍼블릭 아트 중 퍼블릭 아트의 예술성, 환경성, 공공성이 높게 나타났고 환경성도 중간 수준을 보이고 있지만, 역사성이 높지 않고, 상호 작용성이 약하다. 산업 유산물로 재탄생한 퍼블릭 아트는 역사유적 퍼블릭 아트로 산업유산과 도자기 요소로 대중들의 시공간과소통을 형성하면서 대중에게 정신적 공감을 전달할 수 있는 아이덴티티를 형성하였으며, 지역재생을 위한 신 퍼블릭 아트는 도계천에 새로운 예술적 정취를 부여하였으나 새로운 아이덴티티는 나타내지 못하는 것으로 평가되었다.

Cultural Identity that appear in Latin American Fashion: With a focus on the Mexican brands, Taller Flora and F$\acute{a}$brica Social

  • Kim, So-Ra
    • International Journal of Costume and Fashion
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    • 제13권1호
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    • pp.61-79
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    • 2013
  • This study takes a look at the distinct and exclusive culture of Latin America, with a specific focus on Mexico, and attempts to understand its unique cultural identity by comparing, analyzing, and evaluating the characteristics of the Mexican fashion brands, Taller Flora and F$\acute{a}$brica Social, which are brands that have actively used the Latin American cultural identity in its designs. The research method was as follows: 1) performing literature research with specialty publications and thesis that dealt with the two brands 2) analyzing the contents of various articles from the Internet and interviews related to the brands. Thus, this research attempts to understand the characteristics of the fashion brands that use the cultural traits of Latin America in order to dissect its methods and find a way to apply it so that it can be used in different cultures, and in effect, lead to the advancement of the fashion industries, in general. Taller Flora and F$\acute{a}$brica Social are two brands that are able to stand out with a distinct identity by applying traditional native Latin American qualities in its products, as well as its products reflecting a respectful and balanced relationship between people and nature. The characteristics of the two brands are as follows: 1) showing the concept of "the coexistence of diversity" by expressing "the old and the new", "simplicity and flash", and "melding of the Western culture via colonialism and traditional cultures of the aboriginals and other diverse native cultures" 2) achieving "the recovery of the human dignity" by protecting the human rights of the native female artisans and building a network for equality 3) pursuing "free naturalism" by balancing the natural provision of nature and man in addition to promoting environmental protection and conservation of goods 4) becoming "the bridge between communication and exchange" by connecting the artisan, the designer and the consumers together, or the producer and the market, as well as the different cultures of markets from diverse countries.

전통문화 정체성에 관한 조형성 특성과 패션문화상품 개발연구 - Past, Present & Future - (Formative Characteristics Regarding Traditional Cultural Identity and Development of Fashion Culture Products - Past, Present, and Future -)

  • 전지현;앤드류래프터리
    • 복식
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    • 제59권7호
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    • pp.65-76
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    • 2009
  • Korea has established a code for the economic and cultural industry that covers a wide range of classes unlike the old information era which was limited to the simple knowledge, technology, tools, morals, and customers that had been established in our society. On the other hand, 'culture marketing' is a series of marketing activities in which value-added items are created and shared. The combination of business management and culture seemed pretty awkward considering how rapidly the world was changing, however, the nature of today's culture has changed and the economic nature of culture products has resulted from those changes. In this paper, two authors who expressed the cultural identity in the culture products are studied through a comparison analysis; the author Min Yeongsoon expressed the identity confusion that she experienced in a foreign country and how she overcame homesickness in her work; and the author Kim Ata expressed his confidence in Asia and used his clear and loud voice to express the culture in his work. In addition, today's culture products are affected by the surrounding cultures such as the member's traditional society, environmental culture, and individual's culture. It is a good source for good works of art. In this study, the efforts of seeking the cultural identity, structuring the concept, seeking a new market and possibilities are used to make a consistent process in order to make a series of the systems required for planning and developing culture products.

Morphological and Molecular Analyses of $Anabaena$ $variabilis$ and $Trichormus$ $variabilis$ (Cyanobacteria) from Korea

  • Choi, Gang-Guk;Yoon, Sook-Kyung;Kim, Hee-Sik;Ahn, Chi-Yong;Oh, Hee-Mock
    • 환경생물
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    • 제30권1호
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    • pp.54-63
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    • 2012
  • This study characterizes three $Anabaena$ strains and 5 $Trichormus$ strains isolated from Korean waters and 3 $Anabaena$ $flos-aquae$ strains procured from the UTEX based on morphological features and molecular analyses. The $Anabaena$ and $Trichormus$ isolates were morphologically assigned to $A.$ $variabilis$ K$\ddot{u}$tzing and $T.$ $variabilis$(K$\ddot{u}$tzing ex Bornet et Flahault) Kom$\acute{a}$rek et Anagnostidis, respectively. The $Anabaena$ and $Trichormus$ strains differed significantly in the mean length of their vegetative cells. The 16S rRNA genes from the $Anabaena$ strains showed a 100% identity to that from $A.$ $variabilis$ ATCC 29413, while the 16S rRNA genes from the $Trichormus$ strains showed a 99.9% identity to that from $T.$ $variabilis$ GREIFSWALD. The overall topology was in agreement for the 16S rRNA gene and $cpcBA$-IGS trees in the both tree-constructing methods. In a neighbor-joining tree based on the 16S rRNA gene, the 3 $Anabaena$ strains were asso-ciated with $A.$ $variabilis$, the 5 $Trichormus$ strains with $T.$ $variabilis$, and the 3 $Anabaena$ (UTEX) strains were with $Nostoc$. To date, this is the first report on $A.$ $variabilis$ and $T.$ $variabilis$ strains originating from Korea.