• Title/Summary/Keyword: enhanced functionality

Search Result 159, Processing Time 0.022 seconds

Characterization of a Recombinant Thermostable Xylanase from Hot Spring Thermophilic Geobacillus sp. TC-W7

  • Liu, Bin;Zhang, Ningning;Zhao, Chao;Lin, Baixue;Xie, Lianhui;Huang, Yifan
    • Journal of Microbiology and Biotechnology
    • /
    • v.22 no.10
    • /
    • pp.1388-1394
    • /
    • 2012
  • A xylanase-producing thermophilic strain, Geobacillus sp. TC-W7, was isolated from a hot spring in Yongtai (Fuzhou, China). Subsequently, the xylanase gene that encoded 407 amino acids was cloned and expressed. The recombinant xylanase was purified by GST affinity chromatography and exhibited maximum activity at $75^{\circ}C$ and a pH of 8.2. The enzyme was active up to $95^{\circ}C$ and showed activity over a wide pH range of 5.2 to 10.2. Additionally, the recombinant xylanase showed high thermostability and pH stability. More than 85% of the enzyme's activity was retained after incubation at $70^{\circ}C$ for 90 min at a pH of 8.2. The activity of the recombinant xylanase was enhanced by treatment with 10 mM enzyme inhibitors (DDT, Tween-20, 2-Me, or TritonX-100) and was inhibited by EDTA or PMSF. Its functionality was stable in the presence of $Li^+$, $Na^+$, and $K^+$, but inhibited by $Hg^{2+}$, $Ni^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Zn^{2+}$, $Pb^{2+}$, $Fe^{3+}$, and $Al^{3+}$. The functionality of the crude xylanase had similar properties to the recombinant xylanase except for when it was treated with $Al^{2+}$ or $Fe^{2+}$. The enzyme might be a promising candidate for various industrial applications such as the biofuel, food, and paper and pulp industries.

A Research on Design Direction for the Smart Walking Wear to Support Walking Exercise for the Baby Boomer Group (베이비부머 집단을 위한 보행 운동용 스마트 워킹웨어의 디자인 방향 연구)

  • Ban, Hyunsung;Hwang, Sujung;Kim, Sinhye;Lee, Joohyeon
    • Science of Emotion and Sensibility
    • /
    • v.21 no.3
    • /
    • pp.129-140
    • /
    • 2018
  • This study aimed to analyze consumers' acceptance of sports applications and smart sportswear to better design technology-enhanced walking exercise wear for the Baby Boomer generation based on their cohort characteristics. A questionnaire with items related to acceptance of existing smart sportswear design, functionality, and usability as well as existing sports application design, functionality, and usability was sent to consumers aged 50-65. Of 163 questionnaires distributed, 150 were used for analysis. The results showed that middle-aged consumers were aware of smart sportswear's functional stability, but were concerned about care, durability, and convenience. Middle-aged consumers were also aware of sports applications as educational functions for obtaining new information. Additionally, they found sports applications to have lower perceived convenience and accessibility relative to the young generation, highlighting the need for simple instructions and explanations for sports application planning. Based on these results, we propose "Everyday design for general sportswear," "Functions based on consumer's preference," "Enhanced design for durability and management convenience" for designing and planning walking exercise wear for middle-aged consumers, and "Convenient application organization" and "Educational exercise contents" for application planning for walking exercises.

Analysis of nutritional contents and useful functional materials for finding breeding resources in Flammulina velutipes (팽이 기능성 육종소재 발굴을 위한 영양성분 및 유용 기능성 물질 분석)

  • Ji-Hoon, Im;Minji, Oh;Youn-Lee, Oh;Min-Sik, Kim;Jong-Won, Lee
    • Journal of Mushroom
    • /
    • v.20 no.4
    • /
    • pp.218-226
    • /
    • 2022
  • Flammulina velutipes, known as winter mushroom in the family of Physalacriaceae, is the main edible and export mushroom with the third highest production after oyster and king oyster mushroom in Korea. However, as normal consumers regard F. velutipes as a simple subsidiary material, there is a limitation to increasing mushroom demand. In order to overcome the consumption limit and increase the differentiation of new varieties, it is necessary to breed varieties with enhanced functionality in consideration of consumer preferences. Therefore, the study was performed to analyze nutrient components and several useful functional substances with 26 genetic resources of F. velutipes. Analyses of inorganic compound(Ca, K, Mg) and 15 amino acids revealed that Strain 4148 had the highest content among the 26 strains. Beta-glucan, which increases immune activity and polyphenol, which exert antioxidant effects were higher in non-white strains than in white strains with a small number of exceptions. Among the five fatty acids, linoleic acid(an omega-6 fatty acid) and α-linolenic acid(an omega-3 fatty acid), were detected in six mushroom strains. α-linolenic acid, which was not found in five major mushrooms including oyster mushrooms, was identified in F. velutipes. The results of HPLC analysis showed that 'Auram' (Strain 4232) and 'Baekseung'(Strain 4230) had the highest content of the stabilizing neurotransmitter GABA(15.38 ㎍/ml and 20.56 ㎍/ml, respectively) among non-white and white strains, respectively. Our findings provide useful information for breeding F. velutipes to obtain strains with enhanced functionality.

Design of Operating System for Wireless Sensor Nodes with Enhanced Remote Code Update Functionality (원격 코드 업데이트가 가능한 무선 센서 노드용 운영체제)

  • Kim, Chang-Hoon;Cha, Jeong-Woo;Kim, Il-Hyu
    • Journal of Korea Society of Industrial Information Systems
    • /
    • v.16 no.1
    • /
    • pp.37-48
    • /
    • 2011
  • Sensor networks monitor the environment, collect sensed data, and relay the data back to a collection point. Although sensor nodes have very limited hardware resources, they require an operating system that can provide efficient resource management and various application environments. In addition, the wireless sensor networks require the code update previously deployed to patch bugs in program and to improve performance of kernel service routines and application programs. This paper presents EPRCU (Easy to Perform Remote Code Update), a new operating system for wireless sensor nodes, which has enhanced functionalities to perform remote code update. To achieve an efficient code update, the EPRCU provides dynamic memory allocation and program memory management. It supports the event-driven kernel, which uses priority-based scheduling with the application of aging techniques. Therefore, the proposed operating system is not only easy to perform wireless communication with the remote code update but also suitable for various sensor network applications.

Comparisons of Interfacial Reaction Characteristics on Flip Chip Package with Cu Column BOL Enhanced Process (fcCuBE®) and Bond on Capture Pad (BOC) under Electrical Current Stressing

  • Kim, Jae Myeong;Ahn, Billy;Ouyang, Eric;Park, Susan;Lee, Yong Taek;Kim, Gwang
    • Journal of the Microelectronics and Packaging Society
    • /
    • v.20 no.4
    • /
    • pp.53-58
    • /
    • 2013
  • An innovative packaging solution, Flip Chip with Copper (Cu) Column bond on lead (BOL) Enhanced Process (fcCuBE$^{(R)}$) delivers a cost effective, high performance packaging solution over typical bond on capture pad (BOC) technology. These advantages include improved routing efficiency on the substrate top layer thus allowing conversion functionality; furthermore, package cost is lowered by means of reduced substrate layer count and removal of solder on pad (SOP). On the other hand, as electronic packaging technology develops to meet the miniaturization trend from consumer demand, reliability testing will become an important issue in advanced technology area. In particular, electromigration (EM) of flip chip bumps is an increasing reliability concern in the manufacturing of integrated circuit (IC) components and electronic systems. This paper presents the results on EM characteristics on BOL and BOC structures under electrical current stressing in order to investigate the comparison between two different typed structures. EM data was collected for over 7000 hours under accelerated conditions (temperatures: $125^{\circ}C$, $135^{\circ}C$, and $150^{\circ}C$ and stress current: 300 mA, 400 mA, and 500 mA). All samples have been tested without any failures, however, we attempted to find morphologies induced by EM effects through cross-sectional analysis and investigated the interfacial reaction characteristics between BOL and BOC structures under current stressing. EM damage was observed at the solder joint of BOC structure but the BOL structure did not show any damage from the effects of EM. The EM data indicates that the fcCuBE$^{(R)}$ BOL Cu column bump provides a significantly better EM reliability.

A Study on the Development of Women's Golf Windbreaker Design (여성 골프웨어 바람막이 디자인 개발)

  • Ryou, Heun-Sook
    • Journal of the Korean Society of Clothing and Textiles
    • /
    • v.33 no.3
    • /
    • pp.420-432
    • /
    • 2009
  • The purpose of this study is to survey the present women's golf windbreaker market to develop the trendy product with supplementing design and functionality by grasping the inconvenient part through the result of the questionnaire. According to the questionnaire, 38.1% said that the armhole is the most inconvenient part by tightfitting. Therefore, a mobility of wearer was enhanced by giving shirring detail to the armhole for flexibility through inserting the elastic band with a regular interval around armhole in the design1. The swing movement was allowed to be convenient by attaching knit to the side panel. And, the sleeves were designed in the detachable form so those are allowed to be produced in two kinds such as long sleeves and short sleeves. In the design2, a zipper was inserted from the neckline to the armhole so it is designed a detachable bolero. Furthermore, it was designed to be possible to wear two different way as a jumper style and a vest style. Given wearing a vest, the sleeve part was patched knit, thereby having enhanced activity. The design1 and the design2 evaluated an objective assessment and subjective assessment. Assessment group consists of 5 subject groups and 10 expert groups to evaluate external appearance and movement adaptability. In the external appearance evaluation, the design1 obtained the better evaluation than design2. In the movement adaptability evaluation, the design1 obtained the better evaluation than the design2 for all of the items except the wrist area. In the group of professionals, the design1 obtained the better evaluation than the design2 for all of the items except the bottom area.

Synthesis of Hexagonal Boron Nitride Nanocrystals and Their Application to Thermally Conductive Composites (육방정 질화붕소 나노입자 합성 및 열전도성 복합체 응용)

  • Jung, Jae-Yong;Kim, Yang-Do;Shin, Pyung-Woo;Kim, Young-Kuk
    • Journal of Powder Materials
    • /
    • v.23 no.6
    • /
    • pp.414-419
    • /
    • 2016
  • Much attention has been paid to thermally conductive materials for efficient heat dissipation of electronic devices to maintain their functionality and to support lifetime span. Hexagonal boron nitride (h-BN), which has a high thermal conductivity, is one of the most suitable materials for thermally conductive composites. In this study, we synthesize h-BN nanocrystals by pyrolysis of cost-effective precursors, boric acid, and melamine. Through pyrolysis at $900^{\circ}C$ and subsequent annealing at $1500^{\circ}C$, h-BN nanoparticles with diameters of ~80 nm are synthesized. We demonstrate that the addition of small amounts of Eu-containing salts during the preparation of melamine borate precursors significantly enhanced the crystallinity of h-BN. In particular, addition of Eu assists the growth of h-BN nanoplatelets with diameters up to ~200 nm. Polymer composites containing both spherical $Al_2O_3$ (70 vol%) and Eu-doped h-BN nanoparticles (4 vol%) show an enhanced thermal conductivity (${\lambda}{\sim}1.72W/mK$), which is larger than the thermal conductivity of polymer composites containing spherical $Al_2O_3$ (70 vol%) as the sole fillers (${\lambda}{\sim}1.48W/mK$).

Meaurement Algorithms for EDGE Terminal Performance Test (EDGE 단말기 성능 테스트를 위한 측정 알고리즘)

  • Kang, Sung-Jin;Hong, Dae-Ki;Kim, Nam-Yong
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.13 no.12
    • /
    • pp.2719-2730
    • /
    • 2009
  • In this paper, we implement the measurement functionality for performance measurements of EDGE (Enhanced Data Rates for GSM Evolution) terminal by using software. Generally speaking, the receiving algorithms in normal MODEM cannot be used directly to a measurement system due to the lack of accuracy. Therefore, we propose a new receiver algorithm for precise EDGE signal measurements. In the proposed algorithm, 2-stage (coarse stage, fine stage) parameters estimation (symbol-timing, frequency offset, carrier phase) scheme is used. To improve the estimation accuracy, we increase the number of the received signal samples by interpolation. The proposed EDGE signal measurement algorithm can be used for verifying the hardware measurement system, and also can be used for the commercial systems through software optimization.

Effects of Lotus (Nelumbo nucifera) Leaf on Quality and Antioxidant Activity of Yogurt during Refrigerated Storage

  • Kim, Da-Hee;Cho, Won-Young;Yeon, Su-Jung;Choi, Sung-Hee;Lee, Chi-Ho
    • Food Science of Animal Resources
    • /
    • v.39 no.5
    • /
    • pp.792-803
    • /
    • 2019
  • The supplementation with natural ingredients that are rich in polyphenols could improve the quality and functionality of yogurt. Lotus leaf (LL) are abundant in phenolic compounds. We aimed to investigate the effects of LL powder on the quality properties, total phenolic content (TPC), and antioxidant activity of yogurt. Yogurt was supplemented with four different concentrations (0%, 0.2%, 0.5%, and 1%) of LL powder and evaluated for 14 d refrigerated storage. During storage, the titratable acidity (TA) of all LL yogurt groups was higher than that of the control (p<0.05). The $L^*$ and $a^*$ values decreased by LL addition to the yogurts, while the $b^*$ values increased. All LL yogurt groups indicated better viscosity than that of control, and among them, 0.2% LL yogurts had the highest viscosity without significant decrease until the end of the storage duration. The addition of LL into yogurt enhanced the water-holding capacity (WHC) by at least 1.5-fold than that of control for the entire storage duration. The TPC of yogurts gradually increased with the addition of LL (p<0.05) and continued to increase during storage; the 1% LL yogurt at 14 d showed the highest value of $61.94{\pm}1.68{\mu}g$ GAE/g. The DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity and reducing power of the yogurts were also significantly enhanced with increasing LL concentrations (p<0.05). These results suggest that lotus leaf may be useful as a natural ingredient for improving the quality and antioxidant activity of yogurt.

Ginsenoside Rg1 augments oxidative metabolism and anabolic response of skeletal muscle in mice

  • Jeong, Hyeon-Ju;So, Hyun-Kyung;Jo, Ayoung;Kim, Hye-Been;Lee, Sang-Jin;Bae, Gyu-Un;Kang, Jong-Sun
    • Journal of Ginseng Research
    • /
    • v.43 no.3
    • /
    • pp.475-481
    • /
    • 2019
  • Background: The ginsenoside Rg1 has been shown to exert various pharmacological activities with health benefits. Previously, we have reported that Rg1 promoted myogenic differentiation and myotube growth in C2C12 myoblasts. In this study, the in vivo effect of Rg1 on fiber-type composition and oxidative metabolism in skeletal muscle was examined. Methods: To examine the effect of Rg1 on skeletal muscle, 3-month-old mice were treated with Rg1 for 5 weeks. To assess muscle strength, grip strength tests were performed, and the lower hind limb muscles were harvested, followed by various detailed analysis, such as histological staining, immunoblotting, immunostaining, and real-time quantitative reverse transcription polymerase chain reaction. In addition, to verify the in vivo data, primary myoblasts isolated from mice were treated with Rg1, and the Rg1 effect on myotube growth was examined by immunoblotting and immunostaining analysis. Results: Rg1 treatment increased the expression of myosin heavy chain isoforms characteristic for both oxidative and glycolytic muscle fibers; increased myofiber sizes were accompanied by enhanced muscle strength. Rg1 treatment also enhanced oxidative muscle metabolism with elevated oxidative phosphorylation proteins. Furthermore, Rg1-treated muscles exhibited increased levels of anabolic S6 kinase signaling. Conclusion: Rg1 improves muscle functionality via enhancing muscle gene expression and oxidative muscle metabolism in mice.