• Title/Summary/Keyword: enhanced biomass

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Enhanced Cell Growth of Chlorella sp. KR-l by the Addition of Iron and EDTA

  • Sung, Ki-Don;Lee, Jin-Suk;Shin, Chul-Seung;Park, Soon-Chul
    • Journal of Microbiology and Biotechnology
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    • v.8 no.4
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    • pp.409-411
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    • 1998
  • The effects of iron and EDTA on the growth of Chlorella sp. KR-1, a highly$CO_2$tolerant fresh water micro alga, have been determined. The algal growth was significantly affected not only by iron concentrations in the medium but by the ratio of iron to EDTA. The linear growth rate and the final cell concentration are increased with the supplementation of EDTA. Enhanced growth of Chlorella sp. KR-1 by the supplementation of EDTA was mainly due to the fact that the supply of iron to the algal culture had been possible for a longer time. When Chlorella sp. KR-1 is cultured in the medium of iron-15H-EDTA, the linear growth rate and the final cell concentration are at their maximum, 0.88 g/l${\cdot}$day and 9.1 g/l, respectively. The results show that Chlorella sp. KR-1 may be used for mass cultivation to fix$CO_2$from stack gases.

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Development and Evaluation of the Attrition Coupled Bioreactors for Enzymatic Hydrolysis of Biomass; Agitated Bead Type Bioattritor for Enzymatic Hydrolysis of Cellulose (Biomass의 고효율 효소당화에 의한 적합한 Attrition Coupled Bioreactor개발에 관한 연구 ; Agitated Bead Type Bioattritor를 활용한 섬유소 당화)

  • 이용현;박진서;윤대모
    • KSBB Journal
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    • v.4 no.2
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    • pp.78-86
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    • 1989
  • The effective saccharification of cellulosic biomass to glucose is the most critical step for the conversion of renwable biomass to alternative liquid fuel. The enzymatic hydrolysis of biomass can be significantly enhanced provide the attrition milling media is added during hydrolysis. The enhancing mechanism of hydrolysis reaction in an agitated bead system was investigated. An attrition-reactor (bioattritor) which installed specially designed torque measuring apparatus was developed, and the potimal saccharification conditions of bioattritor were determined. The relationship between the power consumption required for agitation of attrition-milling media and enhanced extent of hydrolysis of biomass was compared to evaluatic economic feasibility of the process.

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Effectiveness of Antagonistic Bacterial Metabolites to Control Rhizoctonia solani on Lettuces and Fusarium oxysporum on Tomatoes

  • Vu, Van Hanh;Thi, Quyen Dinh;Rita, Grosch;Dung, Nguyen Ngoc
    • Microbiology and Biotechnology Letters
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    • v.41 no.1
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    • pp.70-78
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    • 2013
  • Rhizoctonia solani and Fusarium oxysporum cause yield losses in numerous economically important crops. To develop a bio-control agent, cell free extracellular compounds (ECs) of 5 bacterial strains Burkholdria sp. L1, Pseudomonas sp. L4, Pseudomonas chlororaphis VN391, Bacillus subtilis VN21 and Enterobacter sp. VN99 from Vietnamese fields, which reduced levels of R. solani root rot in lettuces and F. oxysporum root rot in tomatoes, were investigated. In a growth chamber, ECs of all antagonists markedly enhanced the biomass of lettuces (10 to 14.1%) and tomatoes (11.38 to 13.88%). In greenhouses, the disease's severity on both crops treated with ECs of the antagonists was reduced significantly and biomass losses in the plants decreased markedly. The reduction level of R. solani root rot in lettuces was 75, 66.7, 50, and 16.7% by ECs of strains L1, L4, VN21 and VN391, respectively. The biomass of lettuces increased markedly by 29.13%, 21.67%, and 23.4% by ECs of strains L1, L4 and VN21, respectively. Similarly, the reduction levels of F. oxysporum root rot in tomatoes was 76.3, 75, 41.7 and 25% by ECs of strain L1, L4, VN21 and VN391, respectively, and the biomass was significantly enhanced by 14.42, 12.7 and 13%, respectively. The ECs of strain L1 exhibited the most effective bio-control agents to suppress R. solani and F. oxysporum.

Electrical Energy Production Using Biomass (바이오매스 기반 전기에너지 생산기술 동향 분석)

  • Jongseo Lee;Sang-Soo Han;Doyeun Kim;JuHyun Kim;Sangjin Park
    • New & Renewable Energy
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    • v.19 no.1
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    • pp.12-21
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    • 2023
  • Governments and global companies are working towards using renewable sources of energy, such as solar, wind, and biomass, to reduce dependency on fossil fuels. In the defense sector, the new strategy seeks to increase the sustainable use of renewable energy sources to improve energy security and reduce military transportation. Renewable energy technologies are affected by factors such as climate, resources, and policy environments. Therefore, governments and global companies need to carefully select the optimal renewable energy sources and deployment strategies. Biomass is a promising energy source owing to its high energy density and ease of collection and harvesting. Many techniques have been developed to convert the biomass into electrical energy. Recently, diverse types of fuel cells have been suggested that can directly convert the chemical energy of biomass into electrical energy. The recently developed biomass flow fuel cell has significantly enhanced the power density several hundred times, reaching to ~100 mW/cm2. In this review, we explore various strategies for producing electrical energy from biomass using modern methods, and discuss the challenges and potential prospects of this method.

Enhanced Production of Exopolysaccharides by Fed-batch Culture of Ganoderma resinaceum DG-6556

  • Kim Hyun-Mi;Paik Soon-Young;Ra Kyung-Soo;Koo Kwang-Bon;Yun Jong-Won;Choi Jang-Won
    • Journal of Microbiology
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    • v.44 no.2
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    • pp.233-242
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    • 2006
  • The objectives of this study were to optimize submerged culture conditions of a new fungal isolate, Ganorderma resinaceum, and to enhance the production of bioactive mycelial biomass and exopolysaccharides (EPS) by fed-batch culture. The maximum mycelial growth and EPS production in batch culture were achieved in a medium containing 10 g/l glucose, 8 g/l soy peptone, and 5 mM $MnCl_2$ at an initial pH 6.0 and temperature $31^{\circ}C$. After optimization of culture medium and environmental conditions in batch cultures, a fed-batch culture strategy was employed to enhance production of mycelial biomass and EPS. Five different EPS with molecular weights ranging from 53,000 to 5,257,000 g/mole were obtained from either top or bottom fractions of ethanol precipitate of culture filtrate. A fed-batch culture of G. resinaceum led to enhanced production of both mycelial biomass and EPS. The maximum concentrations of mycelial biomass (42.2 g/l) and EPS (4.6 g/l) were obtained when 50 g/l of glucose was fed at day 6 into an initial 10 g/l of glucose medium. It may be worth attempting with other mushroom fermentation processes for enhanced production of mushroom polysaccharides, particularly those with industrial potential.

Transgenic poplar expressing AtNDPK2 exhibits enhanced biomass in the LMO field

  • An, Chul-Han;Kim, Yun-Hee;Park, Sung-Chul;Jeong, Jae-Cheol;Lee, Haeng-Soon;Choi, Yong-Im;Noh, Eun-Woon;Yun, Dae-Jin;Kim, Se-Bin;Kwak, Sang-Soo
    • Journal of Plant Biotechnology
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    • v.38 no.3
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    • pp.228-233
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    • 2011
  • Nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes and auxin-responsive genes in plants. Previously, it was noted that the overexpression of Arabidopsis NDPK2 (AtNDPK2) under the control of an oxidative stress-inducible SWPA2 promoter in transgenic poplar (Populus alba ${\times}$ P. tremular var. glandulosa) plants (referred to as SN plants) enhanced tolerance to oxidative stress and improved growth (Plant Biotechnol J 9: 34-347, 2011). In this study, growth of transgenic poplar was assessed under living modified organism (LMO) field conditions in terms of biomass in the next year. The growth of transgenic poplar plants increased in comparison with non-transgenic plants. The SN3 and SN4 transgenic lines had 1.6 and 1.2 times higher dry weight in stems than non-transgenic plants at 6 months after planting, respectively. Transgenic poplar also exhibited increased transcript levels of auxin-response genes such as IAA1, IAA2, IAA5 and IAA6. These results suggest that enhanced AtNDPK2 expression increases plant biomass in transgenic poplar through the regulation of auxin-response genes.

Removal of Methylene Blue Using UV-C Pretreated Citrobacter freundii JH 11-2 and Bacillus pseudomycoides JH 2-2 Biomass

  • Gim, HaeWon;Cho, Min;Oh, Byung-Taek
    • Journal of Soil and Groundwater Environment
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    • v.19 no.2
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    • pp.38-43
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    • 2014
  • In this study, we evaluated the methylene blue (MB) adsorption potential of non-treated and UV-C pretreated bacterial biomass from aqueous solution. The UV-C pretreatment denature the biomass and has increased overall functional groups when compared to non-treated biomass. The biosorbent was exposed to various pH, biomass dose, and contact time. The results showed that the dried and UV-C pretreated biomass effectively removed MB within 30 min. Dried and UV-C pretreated biomass of Bacillus pseudomycoides JH 2-2 showed a adsorption of 858.2 and 1072.4 mg/g at optimum conditions (pH: 9.0, contact time: 30 min, biomass dose: 1 g/L). Similarly, dried and UV-C pretreated biomass of Citrobacter freundii JH 11-2 showed an adsorption 868.3 and 954 mg/g at optimum conditions (pH: 9.0, contact time: 10 min, biomass dose: 1.5 g/L). The changes in the functional groups of UV-C pretreated biomass could be responsible for enhanced adsorption of MB. The results obtained have shown that non-treated and UV-C pretreated biomass has a high adsorption capacity for MB dye and can be used as a low-cost biosorbent in wastewater treatments.

Production of Biomass and Lipid Using Microalga Nannochloris oculata Under Different Conditions of Nitrogen and Irradiance (미세조류 Nannochloris oculata의 성장과 지질 생산에 미치는 질소 농도와 광량의 영향)

  • Park, Sang-Jin;Choi, Yoon-E;Kim, Chul-Woong;Park, Won-Kun;Yang, Ji-Won
    • KSBB Journal
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    • v.25 no.6
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    • pp.553-558
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    • 2010
  • Increasing demands on fossil fuel have led to the unprecedented attraction to microalgal biofuel as an alternative energy. In this study, we investigated growth and lipid productions of microalga Nannochloris oculata under various carbon dioxide or nitrogen source concentrations and irradiance conditions. Biomass production of N. oculata was highest under 2% $CO_2$ with 0.3 flow rate (vvm). In addition, biomass productivities were proportional to the concentration of nitrogen source, whereas lipid biosynthesis was suppressed under higher nitrogen concentration (up to 50 mg/L). High irradiation ($160{\sim}180\;{\mu}mol/m^2{\cdot}s$) enhanced growth rate and lipid production of N. oculata.

A Study on the Combustion Characteristics of Biomass using Cone- calorimeter (I) : the Case of Maple Leaves, Gingko Leaves, Bush, Pine Needles (콘 칼로리미터를 이용한 바이오매스의 연소특성에 관한 연구(I) - 단풍잎, 은행잎, 덤불, 솔잎에 대해서 -)

  • Park, Byung-Hyun;Park, Duck-Shin;Cho, Young-Min;Park, Eun-Young;Lee, Cheul-Kyu
    • Journal of Korean Society for Atmospheric Environment
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    • v.21 no.4
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    • pp.459-469
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    • 2005
  • In recent years, concerns have been growing globally regarding greenhouse gases. Open burning of biomass causes emission of a number of greenhouse and other gases and substances. This paper studied an analysis on the characteristics of four types of biomass using duel type cone calorimeter. Cone calorimeter is widely used for assessing combustibility of materials in Europe. As a result, we evaluated several characteristics of biomass, such as heat released rate, smoke production rate, CO, $CO_2$ production and mass loss rate, and so on. $CO_2$ is currently responsible for over $60\%$ of the enhanced greenhouse effect, and may be the most important contributor to future. $CO_2$ production for biomass in the range of $1.74\~1.99kg/kg$ is similar to previous research conducted by Bhattacharya et al. (2002a).

INVESTIGATION OF THE SPECKLE NOISE FILTERS IN ALOS PALSAR IMAGES

  • Amini, Jalal;Sumantyo, Josaphat Tetuko Sri
    • Proceedings of the KSRS Conference
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    • 2008.10a
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    • pp.185-187
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    • 2008
  • Speckle noise is primarily due to the phase fluctuations of the electromagnetic return signals. Since inherent spatial-correlation characteristics of speckle in SAR images are not exploited in existing multiplicative models for speckle noise. In this paper, we investigate the efficiency of the filters: Kuan, Gamma, Enhanced Lee, and Enhanced Frost filters in reduction of speckle noise in forestry area. Selection of the suitable filter can be played an important role in applications such as estimation of biomass in forestry areas. We employ ALOSPALSAR image use L-band frequency for this investigation. The filters test on different sites in PALSAR image of the northern forests part of Iran.

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