• Applied Microscopy
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• v.46 no.2
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• pp.71-75
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• 2016

The scanning electron microscope (SEM) offers two-dimensional (2D) micrographs of three-dimensional (3D) objects due to its inherent operating mechanisms. To overcome this limitation, other devices have been used for quantitative morphological analysis. Many efforts have been made on the applications of software-based approaches to 3D reconstruction and measurements by SEM. Based on the acquisition of two stereo images, a multi-view technique consists of two parts: (i) geometric calibration and (ii) image matching. Quantitative morphological parameters such as height and depth could be nondestructively measured by SEM combined with special software programs. It is also possible to obtain conventional surface parameters such as roughness and volume of biomedical specimens through 3D SEM surface reconstruction. There is growing evidence that conventional 2D SEM without special electron detectors can be transformed to 3D SEM for quantitative measurements in biomedical research.

• 한국전자현미경학회:학술대회논문집
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• 1992.05a
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• pp.5-6
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• 1992

• Proceedings of the IEEK Conference
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• 2009.05a
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• pp.335-337
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• 2009

A scanning electron microscope(SEM) is a system that visualizes complex surface features. The resolution of SEM is affected by each of equipment components. In this study, we examined the effects of the four factors including the beam current, magnification, voltage and working distance. A statistical analysis was conducted to investigate the main and interaction effects. For a systematic characterization, a $2^4$ full factorial experiment was conducted. The $R^2$ of constructed statistical model was 88.9%. The main effect revealed that the current and working distance are dominant factors. Of the interactions, those between the current and voltage yielded the highest interaction. 3D plots generated from the model were used to explore various parameter effects.

• Journal of Energy Engineering
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• v.25 no.1
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• pp.192-197
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• 2016

We have surveyed on technical method of fault analysis of semiconductor device. Fault analysis of semiconductor should first be found the places of fault spots. For this process they are generally used the testers; EB(emission beam tester), EM(emission microscope), OBIRCH(optical beam induced resistance change method) and LVP(laser voltage probing) etc. Therefore we have described about physical interpretation and technical method in using scanning electron microscope, transmission electron microscope, focused ion beam tester and Nano prober.

• Applied Microscopy
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• v.48 no.2
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• pp.49-53
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• 2018

A successful transmission electron microscope (TEM) analysis is closely related to the preparation of the TEM specimen and should be followed by the suitable TEM specimen preparation depending on the purpose of analysis and the subject materials. In the case of the Si-based anode material, lithium atoms of formed Li silicide were removed due to ion beam and electron beam during TEM specimen preparation and TEM observation. To overcome the problem, we proposed a new technique to make a TEM specimen without the ion beam damage. In this study, two types of test specimens from the Si-based anode material of Li-ion battery were prepared by respectively adopting the only focused ion beam (FIB) method and the new FIB-ultramicrotome method. TEM analyses of two samples were conducted to compare the Ga ion damage of the test specimen.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.23 no.3
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• pp.205-211
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• 2013

Objectives: 21 sepiolite substances produced in China were investigated for the presence of asbestos in their materials. Materials and methods: In order to identify asbestos in sepiolite substances, test materials were analyzed using a transmission electron microscope equipped with energy dispersive X-ray spectrometer (TEM-EDS) for confirming their shape and components (atomic %). Results: Five of 21 sepiolte substances were asbestos-containing materials. Two chrysotile containing sepiolite proved to be asbestoscontaining materials, as did two chrysotile mixed with tremolite containing sepiolite. 16 sepiolite substances did not contain asbestos materials. Conclusions: When importing sepiolite substances, they must be analyzed to determine if there is asbestos in their materials.

• Composites Research
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• v.28 no.1
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• pp.1-5
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• 2015

In this paper the influence of fiber orientation of basalt and carbon inter-ply fabrics on the flexural properties of hybrid composite laminates was experimentally investigated. Four types of basalt/carbon/epoxy inter-ply hybrid composite laminates with varying angle ply orientation of reinforced basalt fiber and fixed orientation of carbon fiber were fabricated using hand lay-up technique. Three point bending test was performed according to ASTM 7264. The fracture surface analysis was carried out by scanning electron microscope (SEM). The results obtained from the four laminates were compared. Lay-up pattern of $[0B/+30B/-30B/0C]_S$ exhibits the best properties in terms of flexural strength and flexural modulus. Scanning electron microscopy results on the fracture surface showed that the interfacial de-bonding between the fibers and epoxy resin is a dominant fracture mode for all fiber lay-up schemes.

• Journal of the Korean Chemical Society
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• v.22 no.3
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• pp.184-193
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• 1978

Various aluminum chloride polymeric catalysts have been prepared by the reaction of anhydrous aluminum chloride with styrene-divinylbenzene copolymer bead in the carbon disulfide solvent. The shapes of aluminum chloride polymeric catalysts have been investigated by the use of optical microscope, scanning electron microscope and analysis of atomic absorption and electron microprobe X-ray. The catalytic effect of aluminurn chloride polymeric catalysts in the process of esterification of various organic acids with several aliphatic and aromatic alcohols have been studied.

• Applied Microscopy
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• v.42 no.4
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• pp.218-222
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• 2012

Modern aberration-corrected transmission electron microscope (TEM) with appropriate electron beam energy is able to achieve atomic resolution imaging of single and bilayer graphene sheets. Especially, atomic configuration of bilayer graphene with a rotation angle can be identified from the direct imaging and phase reconstructed imaging since atomic resolution Moir$\acute{e}$ pattern can be obtained successfully at atomic scale using an aberration-corrected TEM. This study boosts a reliable stacking order analysis, which is required for synthesized or artificially prepared multilayer graphene, and lets graphene researchers utilize the information of atomic configuration of stacked graphene layers readily.

• The Journal of Korean Medicine
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• v.20 no.3 s.39
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• pp.27-35
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• 1999

The purpose of this study was to investigate the histochemical effect of Jojoongikgi-tang (JIT) on hairless mice induced by DNCB. For the study, DNCB was applied on the infrascapular region of the mices skin and then JIT was orally administered. As a result, the following histochemical changes of the dermis were observed through light and electron microscopes, and statistical data. The results obtained were as follows: 1. In the 2 days experimental group, more histamine mast cells dermis occurred than in the control or normal group when observed under light microscope. When an electron microscope was used, histochemical reactive cells in the dermis were found as mast cells that contained more cytoplasmic granules than in the control or normal group. 2. In the 5 days experimental group, the number of mast cells were decreased over the control or normal group when observed under light microscope. When an electron microscope was used, mast cells in the control contained secretory granules with higher electron density than those of the experimental group. 3. As a result of statistical analysis, the mean value of mast cells in the normal 2 days control and experimental groups was not significantly different (p<0.05). 4. However, the mean values of it in the normal ($19.1000{\pm}6.3154$), 5 day control ($103.4500{\pm}42.2704$) and 5 day experimental groups ($35.9500{\pm}8.5746$) were significantly different (p<0.05). From the above results, it is concluded that JIT is efficient against the purpura induced by DNCB.