• Parasites, Hosts and Diseases
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• v.38 no.2
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• pp.51-57
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• 2000

Follow-up studies have been conducted every three years on the endemicity of Gymnophalloides seoi infection in a small coastal village of Chollanam-do (Province), Korea, since it was first known as an endemic area in 1994. Special attention was given to its egg laying capacity in the human host. In fecal examinations, the overall helminth egg and/or cyst positive rate was 78.7% (74/94) in 1997 and 76.6% (82/107) in 2000. Among them G. seoi eggs showed the highest rate; 71.3% (67/94) in 1997 and 72.0% (77/107) in 2000. The average number of eggs per gram of feces (EPG) was 1,015 in 1997, while a reduced rate of 353 was observed in 2000. In 1997, total of 320,677 adult flukes of G. seoi (av. 10,344/person, 94-69,125 in range) were collected from the diarrheic stools of 31 treated patients. The EPG/worm obtained from 21 cases ranged from 0.04 to 0.77 (av. 0.23), suggesting density-dependent constraints on the worm fecundity. The relationship between the worm burden (X) and EPG/worm (Y) can be expressed as $Y=0.42{\cdot}e{\cdot}^{-l.2x}(r=0.49)$. The results showed that G. seoi infection is persistently endemic in this village.

• Parasites, Hosts and Diseases
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• v.51 no.6
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• pp.719-726
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• 2013

Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.

• Journal of Korea Water Resources Association
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• v.36 no.6
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• pp.1047-1058
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• 2003

Seasonal occurrence of high ammonia nitrogen(NH3-N) concentrations has hampered chemical treatment processes of a water plant that intakes water at Buyeo site of Geum river. Thus it is often needed to quantify the effect of Daecheong Dam ouflow on the mitigation of $NH_3$-N contamination. In this study, multiple regression models were developed for forecasting daily $NH_3$-N concentrations using 8 years of water quality and dam outflow data, and verified with another 2 years of data set. During model development, the coefficients of determination($R^2$) and model efficiency($E_{m}$) were greater than 0.95. The verification results were also satisfactory although those statistical indices were slightly reduced to 0.84∼0.94 and 0.77∼0.93, respectively. The validated model was applied to assess the effect of different amounts of dam outflow on the reduction of $NH_3$-N concentrations in 2002. The NH3-N concentrations dropped by 0.332∼0.583 mg/L on average during January∼March as outflow increases from 5 to 50cms, and was most significant on February. The results of this research show that the multiple regression approach has potential for efficient cause and effect analysis between dam outflow and downstream water quality.

• Korean Journal of Microbiology
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• v.30 no.2
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• pp.101-107
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• 1992

Cytochrome c oxida5e from chemotrophically grown R p , geliitinosu was purified by cytochrome c affinity chromatography and DEAE-Sephacel ion exchange chromatography. The molecular weight of the cytochrome c oxidase was approximately 110.000 Da by sephacryl s-300 gel chromatography and approximately 52, 000 Da by SDS-gel electrophoresis, respectively. Therefore. cytochrolne c oxidase of Rps. gehtinosu seems to be dimer. The cytochrome c oxidasc was very sensitive to temperature. It's Km and Vmax were 20 pM and 44 unitlmg protein for horsc heart cytochrome c as a substrate. respectively, and its optimum pH and temperature were 6.4 and 25$^{\circ}$C. respectively. The absorption peaks of the reduced cytochrome c oxidase showed at 554 nm, 523 nm. and 422 nm. The activiiy of cytochrome c oxidase was inhibited by KCN, and NaN3, but not by CO, antimycir~ A. and myxothiazol. The cytochrome c-551 was produced either in phototrophically or chemotrophically grown Rps. gelaiinosci. The rcduced cytochrome c-551 was oxidized by b-type cytochrome c oxidase from Rp.v. gc.lrtino.sc~. Km and Vmax of cytochrome c oxidase was 26 pM and 31 unitlnlg protein For cytochrome c-551 as a substrate. respectively. Thercfore. thc electron transfer chain of chemotrophically grown Rps. glatinosa seems lo be ubiquinol cytochrome bc, complex -'cytochrome c-55lMb-type cytochrome c oxidase+02.

• The Korean Journal of Mycology
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• v.9 no.3
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• pp.123-128
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• 1981

Three edible species, e.g. Lyophyllum sp. and Armillaria mellea introduced from Japan in 1979 and Auriaularia auricula-judae from Taiwan in 1978 were selected for artifcial cultivation. The media, MSDS for Lyophyllum sp, MHS and PDS for A. auricula-judae, and MSDS and MHS for A. mellea were selected. The range of optimum pH of Lyophyllum sp. was 6.0 and pH 8.0 for A. auricula-judae and pH 6.0 to 7.0 for A. mellea. The range of optimum temperature for the mycelial growth of Lyophyllum sp. was 25 to $30^{\circ}C,\;30^{\circ}C$ for A. auricula-judae and $25^{\circ}C$ for A. mellea. When the moisture content 75 to 80% (w/w) in the substrates, the mycelial growth of Lyophyllum sp. and A. auricula-judae, was rapid. 65% (w/w) for A. mellea. As the spawning substrates, wheat for Lyophyllum sp. oak sawdust for A. auricula-judae and poplar sawdust for A. mellea were selected, respectively. In this experiment, yields of Lyophyllum sp. and A. auricula-judae were high on the poplar sawdust added 20% (w/w) of rice bran. Lyophyllum sp. and A. auricula-judae for sources of new edible mushrooms seems to be possible.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.5
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• pp.752-757
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• 2003

This study was carried out in order to develop the method for isolation of red ginseng acidic polysaccharide (RGAP) haying immunomodulating antitumor activity from red ginseng by-product. The red ginseng by-product was obtained from red ginseng residues produced in processing of red ginseng ethanol extract. The yield of RGAP isolated by ultrafiltration was 20.9%. The active substance (GFP) was purified by DEAE-sepharose column chromatography RGAP induced nitric oxide (NO) exhibited tumoricidal activities against P8l5 (mastocytoma) tumor cells. Acid-hydrolyzed RGAP fragments were shown four to five spots. These sopts showed the same R$_{f}$ values with sugars designated as rhamnose, glucose, glactose and glucuronic acid. Some physico-chemical properties of RGAP were investigated. pH and dry reduction content at 105$^{\circ}C$ were 4.74 and 4.72%, respectively. Crude protein, ash and Pb contents were 3.30%, 4.74% and 2.30 ppm. These results suggest that we will be able to produce RGAP from red ginseng by-product by ultrafiltration in a large scale.e.

• Journal of the Korea Convergence Society
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• v.6 no.4
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• pp.1-7
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• 2015

This study is a case study of the research study and education with high school students of natural sciences. Recent development of the Internet of Things (IoT) based on the subject of various studies exist. This study is one of the most portable communication devices of these infancy, yet students were easy to define the molding process of contacting a pager that can go along way in making research topics. We called a pager. Today, it is given a large and complex smart mobile communication devices that can be used for big data. Prior pagers are taught the meaning of the first mobile communication means in our lives were given device. The internal structure is relatively simple and is thought to function relatively simple, just suitable as a teaching practices of high school students with an interest in science and engineering universities. This study can see all of the mechanical, electronic information contents of the radio pager in a relatively simple analysis of the injection molding ONE CAVITY analysis. Furthermore, the students also rated good educational practices that give meaning to remind the convergence training on mass production and process automation.

• Proceedings of the Korean Information Science Society Conference
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• 2004.10c
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• pp.631-633
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• 2004

본 논문에서는 유비쿼터스 홈 네트워킹 환경에서 사용자의 위치 이동을 실시간 추적하고 가정 내 주거 활동의 편의를 제공하는 정보가전기기들을 제어할 수 있는 능동형 홈 서비스 시스템을 제안한다. 본 시스템은 다계층의 분산시스템 환경으로 구성된다. 즉, 물리 센서와 정보가전기기 등이 존재하는 물리층과 유/무선 지원 인터넷/인트라넷 통신 플랫폼을 포함하는 시스템층, 그리고 물리적 장치들로부터 시스템층을 통해 들어오는 정보를 처리하는 응용층으로 구성되며 실 생활 공간 및 시간을 응용 시뮬레이션 환경으로 반영시킨다. 능동형 홈 서비스 시스템의 개발 목적은 실버아파트에서 독거노인의 위치기반 건강관리 정보 서비스 및 아파트 내 정보가전기기들의 맞춤형 제어를 제공하기 위함이다. 이와 같은 시스템을 구축하기 위해서는 홈 네트워크 내에서 사용자의 이동 위치를 파악하는 실시간 위치추적 기술과 정보가전기기들의 동작을 실시간 제어하기 위한 기술이 요구된다. 제안한 시스템에서는 UC at Irine의 DREAM Lab.에서 개발한 TMO(Time-triggered Message -triggered Objecl) 스킴을 적용하여 각 물리 센서와 정보가전기기들을 응용의 구성요소로 개발했다. 이를 통해 가정 내에 이동하는 TMO로 매핑된 이동객체를 추적하고 또한 홍 네트워크로 연결된 정보가전기기들을 정보가전 TMO 동작객체로 매핑하여 이들 사이의 능동적인 상호동작을 통해 맞춤형 서비스 및 실시간 제어가 가능하도록 했다. 마지막으로, 실시간 위치추적 및 정보가전제어 응용 시뮬레이션을 통해 능동형 홈 서비스 시스템을 구성하는 개별 동작객체들의 기능성과 수행성을 검증했다.황에 대하여 소개한다.이스는 실험정보가 저장된 데이터베이스, 분석결과가 저장된 데이터베이스, 그리고 유전자 정보 탐색을 위한 데이터베이스로 분류해 데이터를 효율적으로 관리할 수 있게 하였다. 본 시스템은 LiNUX를 운영체계로 하고 데이터베이스는 MYSQL로 하여 JSP, Perl. 통계처리 언어인 R로 구현되었다.프트웨어를 사용하지 않고도 국내의 순수 솔루션인 리눅스 기반의 LonWare 3.0 다중 바인딩 기능을 통해 저 비용으로 홈 네트워크 구성 관리 서버 시스템 개발에 대한 비용을 줄일 수 있다. 기대된다.e 함량이 대체로 높게 나타났다. 점미가 수가용성분에서 goucose대비 용출함량이 고르게 나타나는 경향을 보였고 흑미는 알칼리가용분에서 glucose가 상당량(0.68%) 포함되고 있음을 보여주었고 arabinose(0.68%), xylose(0.05%)도 다른 종류에 비해서 다량 함유한 것으로 나타났다. 흑미는 총식이섬유 함량이 높고 pectic substances, hemicellulose, uronic acid 함량이 높아서 콜레스테롤 저하 등의 효과가 기대되며 고섬유식품으로서 조리 특성 연구가 필요한 것으로 사료된다.리하였다. 얻어진 소견(所見)은 다음과 같았다. 1. 모년령(母年齡), 임신회수(姙娠回數), 임신기간(姙娠其間), 출산시체중등(出産時體重等)의 제요인(諸要因)은 주산기사망(周産基死亡)에 대(對)하여 통계적(統計的)으로 유의(有意)한 영향을 미치고 있어 $25{\sim}29$세(歲)의 연령군에서, 2번째 임신과 2번째의 출산에서 그리고 만삭의 임신 기간에, 출산시체중(出産時體重) $3.50{\

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.179-188
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• 2017

White-rot basidiomycetes are the organisms that decompose lignin most efficiently, and Trametes villosa is a promising species for ligninolytic enzyme production. There are several publications on T. villosa applications for lignin degradation regarding the expression and secretion of laccase and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breakdown of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA, and the corresponding lignin peroxidase (TvLiP) protein from T. villosa strain CCMB561 from the Brazilian semiarid region. The presence of ligninolytic enzymes produced by this strain grown in inducer media was qualitatively and quantitatively analyzed by spectrophotometry, qPCR, and dye fading using Remazol Brilliant Blue R. The spectrophotometric analysis showed that LiP activity was higher than that of MnP. The greatest LiP expression as measured by qPCR occurred on the $7^{th}$ day, and the ABSA medium (agar, sugarcane bagasse, and ammonium sulfate) was the best that favored LiP expression. The amplification of the TvLiP gene median region covering approximately 50% of the T. versicolor LPGIV gene (87% identity); the presence of Trp199, Leu115, Asp193, Trp199, and Ala203 in the translated amplicon of the T. villosa mRNA; and the close phylogenetic relationship between TvLiP and T. versicolor LiP all indicate that the target enzyme is a lignin peroxidase. Therefore, T. villosa CCMB561 has great potential for use as a LiP, MnP, and Lac producer for industrial applications.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.12
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• pp.1689-1695
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• 2008

Leptin, the hormone product of the obese gene, is secreted predominately from white adipose tissue and regulates feed intake, energy metabolism and body composition. It has been considered a candidate gene for performance, carcass and meat quality traits in beef cattle. The objective of this study was to identify SNPs in the promoter region of the leptin gene and to evaluate the possible association of the SNP genotypes with carcass and meat quality traits in Korean cattle. We identified a total of 25 SNPs in the promoter region (1,208-3,049 bp upstream from the transcription start site) of the leptin gene, eleven (g.1508C>G, g.1540G>A, g.1545G>A, g.1551C>T, g.1746T>G, g.1798ins(G), g.1932del(T), g.1933del(T), g.1934del(T), g.1993C>T and g.2033C>T) of which have not been reported previously. Their sequences were deposited in GenBank database with accession number DQ202319. Genotyping of the SNPs located at positions g.2418C>G and g.2423G>A within the promoter region was performed by direct sequencing and PCR-SSCP method to investigate the effects of SNP genotypes on carcass and meat quality traits in Korean cattle. The SNP and SSCP genotypes from the two mutations of the leptin promoter were shown to be associated with the BF trait. The average BF value of animals with heterozygous SNP genotype was significantly greater than that of animals with the homozygous SNP genotypes for the g.2418C>G and g.2423G>A SNPs (p<0.05). Analysis of the combined genotype effect in both SNPs showed that animals with the AC SSCP genotype had higher BF value than animals with BB or AA SSCP genotypes (p<0.05). These results suggest that SNP of the leptin promoter region may be useful markers for selection of economic traits in Korean cattle.