• Title/Summary/Keyword: eIF5B

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An analysis of the enteropathogenic bacteria isolation during the last five years (최근 5년간 분리된 enteropathogenic bacteria)

  • Chong, Yun-Sop;Song, Kyung-Soon;Yi, Kui-Nyung;Lee, Sam-Uel Y.
    • The Journal of the Korean Society for Microbiology
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    • v.14 no.1
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    • pp.17-25
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    • 1979
  • Bacteriologic diagnosis of enteric infection remains to be an important role of clinical laboratory because of the prevalence of the infection. Often the determination of etiologic agent and its susceptibility to antibiotics are of vital importance for a proper management of the infection. In our previous paper, an analysis of the isolation of enteric pathogens for the years 1969-73 was reported to clarify the status of those years. The present analysis was made based on the data obtained during the years 1974-78, to see if any change of the status was rendered. 1. During the 5-year period, from the cultures of 7,308 stool or rectal specimens 833 patients yielded enteric pathogens: 468 Shigella, 295 Salmonella, 30 Vibrio parahaemolyticus and 40 enteropathogenic Escherichia coli(EPEC). 2. Of the 295 Salmonella, 271 were S. typhi Isolation of 12 S.paratyphi-A, 1 Salmonella group B, 4 group C, 5 group D and 2 group E meant a definite increase of these sero-groups, S. typhi was most frequently isolated in August and in December, and from 30- to 39-year-old patients. 3. Of the 468 Shigella, 10 were subgroup A, 338 subgroup B, 3 subgroup C and 117 subgroup D. Most of the subgroup B belonged to type 1,2, or 3. The proportion of S. sonnei decreased from 31.3% in 1974 to 18.2% in 1978. In foreign patients, S. sonnei remained to be the frequntly isolated species. Shigella isolation was frequent in August and in 2- to 5-year-old patients. 4. V. parahaemolyticus was isolated from 30 and EPEC from 40 patients. 5. Ninty-nine per cent and 99.5% of the S. typhi isolates were susceptible to chloramphenicol and to ampicillin respectively. 92.8% of S sonnei were susceptible to ampicillin. S. flexneri type 2 was notable for their markedely decreased proportion being susceptible to ampicillin: 84.4% in 1974 and 25.6% in 1978.

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Effects if Benzo(a)pyrene on Natural Killer Cell Activity of Mice (Benzo(a)pyrene이 마우스 자연살해세포 활설에 미치는 영향)

  • Oh, Dong-Il;Kim, Kwang-Hyuk;Lee, Chung-Han;Chung, Hyun-Kee;Park, Jae-Sun
    • Journal of Life Science
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    • v.8 no.3
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    • pp.257-262
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    • 1998
  • Benzo(a)pyrene(B(a)P), an extensively studied polycyclic aromatic hydrocarbon(PAH), is a common contaminant produced through the burning of fossil fuels, particularly coal, and from the exhaust products of internal combustion engines. It produces a wide range of toxicities, including carcinogenicity in experimental animals. B(a)P has been shown to suppress systemic immunity in experimental animals, which may contribute to the growth of the chemical-induced tumors. Using colorimetric MTT assay natural killer(NK) cell-mediated growth inhibition of tomor cell was measured in normal and B(a)P-exposed C57BL/6 mice. Non-adherent splenocytes of normal or B(a)P-exposed mice were cultured with Yac-1 cells at four different effector/target(E/T) cell ratios ranging from 200/1, 100/1, 50/1, and 25/1 in an assay volume of 0.1 ml. After the optical density of culture wells containing MTT solution was measured at a wavelength of 540 nm, the percentage of dead cells relative to the control target cell number was calculated. The NK activity of B(a)P-exposed mice was markedly lower than that of non-exposed mice group at all E/T ratios. These results indicated that suppression of NK cell activity may play a role in allowing for the growth of tumors.

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A comparative analysis of volatile organic compound levels in field samples between different gas chromatographic approaches (분석기법의 차이에 따른 현장시료의 VOC 분석결과 비교연구: 분석오차의 발생 양상과 원인)

  • Ahn, Ji-Won;Pandey, Sudhir Kumar;Kim, Ki-Hyun
    • Analytical Science and Technology
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    • v.23 no.5
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    • pp.465-476
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    • 2010
  • In this study, a number of volatile organic compounds (VOCs) including benzene, toluene, p-xylene, styrene, and methyl ethyl ketone were analyzed from samples collected in ambient air and under the field conditions. These samples were analyzed independently by two different set-ups for VOC analyses, i.e., between [1] gas chromatography/flame ionization detector with tube sampling - (F-T system) and [2] gas chromatography/mass spectrometer with bag sampling (M-B system). The analytical results derived by both systems showed fairly similar patterns in relative sense but with moderately large differences in absolute sense. The results of M-B system were high relative to F-T system with the F-T/M-B ratio below 1. If the relative biases of the two measurement techniques are derived in terms of percent difference (PD) in concentration values, the results were generally above 35% on average. A student t-test was applied to investigate the statistical significance of those differences between the systems. The results of both analytical systems were different at 95% confidence level for toluene, p-xylene, styrene, and methyl ethyl ketone (P < 0.043). However, F-T and M-B systems showed strong correlations for toluene and p-xylene. The observed bias is explained in large part by such factors as the differences in standard phases used for each system and the chemical loss inside the bag sampler.

Antimutagenic Effects of Methyl Alcohol Extracts from Auricularia auricula and Gyrophora esculenta (목이 및 석이 메틸 알콜 추출물의 항돌연변이원성)

  • Ham, Seung-Shi;Kim, Deug-Ha;Lee, Deuk-Sik
    • Korean Journal of Food Science and Technology
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    • v.29 no.6
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    • pp.1281-1287
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    • 1997
  • This study was conducted to investigate the antimutagenic effects of methyl alcohol extracts from Auricularia auricula and Gyrophora esculenta on the SOS response induced by 4-nitroquinoline-1-oxide (4NQO), N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), mitomycin C (MMC) and 3-amino-1,4- dimethyl-5H-pyrido-[4,3-b]indol (Trp-P-1) in E. coli PQ37/plasmid pKM101. In the mutagenic test on test strain, both methyl alcohol extracts did not show mutagenic activity. In the antimutagenic test, each sample strongly inhibited the mutagenecity induced by 4NQO, MNNG, MMC and Trp-P-1. Methyl alcohol extracts from Auricularia auricula and Gyrophora esculenta showed inhibitory effects of 52% and 59% against 4NQO, 49% and 58% against MNNG, 53% and 64% against MMC, and 61% and 64% against Trp-P-1, respectively. Gyrophora esculenta extracts on the antimutagenicity showed relatively higher inhibitory effects than that of Auricularia auricula.

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Joint Phase and Frequency Offset Estimator for Short Burst MPSK Transmission with Preamble

  • Kim Seung-Geun;Lim Young-Kon
    • The Journal of the Acoustical Society of Korea
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    • v.24 no.4E
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    • pp.152-157
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    • 2005
  • In this paper, a new data-aided joint phase and frequency estimator, which has very low computational complexity, is proposed and its variances of phase and frequency estimates are derived. To estimate the phase and frequency offset, first of all, the overall observation interval is divided into same length sub-intervals, and then phase estimates are independently computed based on symbols of the each sub-intervals. To be continue the sequence of computed phase estimates, proper integer multiples of $2{\pi}$ are added to (or subtracted from) the computed phase estimates, which is called linearized phase estimate. The phase offset of the proposed joint estimator is estimated by averaging the linearized phase estimates and the frequency offset by averaging the differences between consecutive linearized phase estimates. The variance of the proposed phase offset estimate is same to MCRB of phase if there is no frequency offset, but it is smaller than MCRB of phase if there is frequency offset. However, the variance of the proposed frequency offset estimate is bigger by at least 0.5 dB than MCRB of frequency with the same observation interval.

COFINITENESS OF GENERAL LOCAL COHOMOLOGY MODULES FOR SMALL DIMENSIONS

  • Aghapournahr, Moharram;Bahmanpour, Kamal
    • Bulletin of the Korean Mathematical Society
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    • v.53 no.5
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    • pp.1341-1352
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    • 2016
  • Let R be a commutative Noetherian ring, ${\Phi}$ a system of ideals of R and $I{\in}{\Phi}$. In this paper among other things we prove that if M is finitely generated and $t{\in}\mathbb{N}$ such that the R-module $H^i_{\Phi}(M)$ is $FD_{{\leq}1}$ (or weakly Laskerian) for all i < t, then $H^i_{\Phi}(M)$ is ${\Phi}$-cofinite for all i < t and for any $FD_{{\leq}0}$ (or minimax) submodule N of $H^t_{\Phi}(M)$, the R-modules $Hom_R(R/I,H^t_{\Phi}(M)/N)$ and $Ext^1_R(R/I,H^t_{\Phi}(M)/N)$ are finitely generated. Also it is shown that if cd I = 1 or $dimM/IM{\leq}1$ (e.g., $dim\;R/I{\leq}1$) for all $I{\in}{\Phi}$, then the local cohomology module $H^i_{\Phi}(M)$ is ${\Phi}$-cofinite for all $i{\geq}0$. These generalize the main results of Aghapournahr and Bahmanpour [2], Bahmanpour and Naghipour [6, 7]. Also we study cominimaxness and weakly cofiniteness of local cohomology modules with respect to a system of ideals.

Extracellular Production of Alpha-Interferon by Recombinant Escherichia coli : Part I. Construction of Expression Vectors (유전자 재조합 대장균을 사용한 Alpha-interferon의 생산과 분비: 제 1 부. 발현벡터의 제작)

  • 노갑수;최차용
    • KSBB Journal
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    • v.5 no.1
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    • pp.49-58
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    • 1990
  • We constructed hybrid plasmids to allow controlled and extracellular production of human alpha-interferon in Escherichia coli. The hybrid plassmids were constructed by transferring alpha-lFN gene from plasmid Hif-2h which has the alpha-lFN gene at PstI restriction site of pBR322, to plasmids pIN -IIIB3 and pIN-IIIC3 at restriction sites between HindIII and BamHI. Plasmids pIN-IIIB3 and pIN-IIIC3 carry E. coli lipoprotein promoter, lac promoter and operator in tandem. The plasmids also have lacl genes which encode for lac repressors, which allows controlled expression of genes cloned to the plasmids by using of inducer IPTG. Lipoprotein signal sequence is located just ahead of cloning sites of the plasmids, which helps cells to excrete or secrete cloned gene products. Plasmid pUC9 was used as a intermediate vector for transferring of alpha-lFN gene from Hif-2h to pIN vectors in order to solve the problem of different restriction sites between Hif-2h and pIN vectors.

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Comparison of IIR Filter and Wavelet Filter on Acoustic Decay Measurements (음 감쇠 측정에서의 IIR 필터와 웨이블렛 필터의 영향에 대한 수치 계산, 비교)

  • 이상권;이민성;김봉기
    • The Journal of the Acoustical Society of Korea
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    • v.20 no.5
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    • pp.5-13
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    • 2001
  • It is well known that there are two experimental errors on acoustic decay measurements. ,One is due to the influence of the band pass filter the other one is that of an averaging device. In this paper the influence of the filter is investigated in detail. To minimize the influence of the filter, the product of the filter bandwidth B (3dB bandwidth) and the reverberation time T/sub 60/ of the room under test should be at least 16. Moreover, if the initial part of an acoustic decay curve is important, the strong requirement, i. e. BT/sub 60/〉64, must be satisfied. In this paper, the wavelet filter bank instead of the band pass filter bank is applied to obtain an acoustic decay curve. As a result, the influence of filter is reduced and then the value of BT/sub 60/ required for obtaining an acceptable decay curve becomes at least 4. The strong requirement for the initial part of a decay curve is also replaced by the BT/sub 60/〉16 instead of BT/sub 60/〉64.

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Studies on storing Chest - nut(Castanea crenata var. dulcis Nakai) Sealing with Polyethylene Film (밤의 Polyethylene Film 밀봉 저장 효과)

  • Lee, B.Y.;Yoon, I.H.;Kim, Y.B.;Han, P.J.;Lee, Ch.M.
    • Korean Journal of Food Science and Technology
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    • v.17 no.5
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    • pp.331-335
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    • 1985
  • Series of study were conducted to develop a method for longterm storage of chest-nut with preserving it's taste and freshness. Experiments were carried out with Korean chest-nut (Castanea crenata var. Okkwang) sealed in polyethylene (P.E) film stored under the ambient and low temperature. Summarized results are as follow: After the harvest, $CO_2$ produced by chest-nut at the early storage was increased with temperature increase. Q10 mg/kg/day, the temperature index of $CO_2$production, by chest-nut ranged 2.4-2.7. It was available to store chest-nut in good condition with 8-15% total loss upto the following may at the ambient temperature sealed in 0.03 mm P.E. film, and upto the following july at the low temperature if sealed in 0.03 or 0.05 mm P.E. film. Throughout the period from one month after the innitiation upto the end of the storage, the rate of $CO_2$and $O_2$ was maintained near the optimum condition for the CA storage of chest-nut. The taste of chest-nut was improved during the storage due to increased reducing-sugar and decreased wate soluble tannin. However, the taste become bitter and unacceptable from the early stage of the storage when used the thicker P.E. film (than above mentioned) for the sealing.

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FINDING THE SKEW-SYMMETRIC SOLVENT TO A QUADRATIC MATRIX EQUATION

  • Han, Yin-Huan;Kim, Hyun-Min
    • East Asian mathematical journal
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    • v.28 no.5
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    • pp.587-595
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    • 2012
  • In this paper we consider the quadratic matrix equation which can be defined be $$Q(X)=AX^2+BX+C=0$$, where X is a $n{\times}n$ unknown real matrix; A,B and C are $n{\times}n$ given matrices with real elements. Newton's method is considered to find the skew-symmetric solvent of the nonlinear matrix equations Q(X). We also show that the method converges the skew-symmetric solvent even if the Fr$\acute{e}$chet derivative is singular. Finally, we give some numerical examples.