• Information Systems Review
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• v.7 no.1
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• pp.115-136
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• 2005

Recently Internet portals contend with each other to expand their customer base by providing customers with a much amount of e-mail storage. But This strategy may not be successful because of customers' switching cost following site moving. Therefore, in order to reveal whether their strategy makes sense, this study investigates the direct and contingency factors affecting customer switching intension of e-mail services in portal sites. The research results show that attractiveness of alternatives, switching cost, and customer satisfaction have a significantly direct impact on customer switching intension of e-mail services. Also, attractiveness of alternatives alone has an moderated effect on customer switching intension but switching cost does not. Thus, Internet portals may reap the harvest from their recent aggressive action.

• The Journal of Society for e-Business Studies
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• v.10 no.1
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• pp.103-120
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• 2005

The purpose of this research is to provide strategic guidelines for EDI transaction companies to migrate from EDI system to Web-based e-Business frameworks such as ebXML, RosettaNet and Web Services. Due to various limitations of EDI and evolution of Web technologies, a lot of firms want to adopt Internet based B2B standards However, they are seeking ways of migrating from EDI to open and public B2B frameworks smoothly not giving up existing EDI system at a time. Therefore, it is very important to provide migration strategies and guidelines for companies to migrate from EDI system to Web-based B2B frameworks. The strategic guidelines provided in this paper can be used as a practical guidance in developing migration strategies of EDI companies.

• International Commerce and Information Review
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• v.13 no.4
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• pp.23-49
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• 2011

The of this study was to establish a theoretical basis for relationship marketing exercised in the mB e-Marketplace and to empirically analyze causal relationship among e-CRM factor, together with quality and the performance of the customer relationship. The population sample of this study was the companies who had experience for using the B2B e-Marketplace, and a data analysis was made through y analysis, factor analysis, multiple regression analysis and hierarchical regression analysis, utilizing statistical package of SPSS 18.0. The result of proving the are as follows; First, the e-CRM factors(e-Marketing, e-Service, e-Sales) of B2B e-Marketplace showed the positive influence on the customer relationship quality factor. the e-CRM factors of B2B e-Marketplace showed the positive influence on the customer relationship performance factor. Third, according to the test result on the relationship between quality factor and performance factor, customer relationship quality factor(satisfaction, trust) had positive effect on performance factor. Last, for the moderating effect of switching barrier in the customer relationship quality factor(satisfaction, trust) on the B2B e-Marketplace and its performance factor(reusing intention, long-term orientation) of maintaining the relation, the subordinate variables of switching barrier(alternative attraction, switching cost) showed the positive moderating effects.

• Proceedings of the CALSEC Conference
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• 2001.02a
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• pp.31-35
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• 2001

□ 정보 단절로 인한 업무 Loss제거 □ 공정하고 투명한 업무 Process 기대 □ 다양한 형태의 정보공유방법 요구 □ 기존 시스템의 본부별 이질화 □ 협력회사 사용 시스템이 분산 □ e-SCM 실행 전략 Model접근 선택 -e-Procurement로의 Transform요구 □ Analog에서 Digital 기업으로의 변경요구 □ 고객 중심적 Business 전환 □ 정보를 실시간으로 공유할 수 있는 기술 e-Procurement 체제로의 업무 전환 o 사용자 위주의 시스템 구현 o 업무단위별 2way 실현으로 효율증진 o 상호 실시간 정보공유를 통한 win-win 실현 o 신뢰성/투명성 제고 o 협력회사용 접속 Channel Portal화 Paperless ＆ Real Time(중략)

• Korean Journal of Plant Resources
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• v.20 no.5
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• pp.437-441
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• 2007

The argE gene of E.coli was introduced into #Ilmibyeo# cultivar of rice by Agrobacterium tumefaciens and a large number of transgenic plants were produced. Embryogenic calli were co-cultivated with A. tumefaciens strain AGL1 carrying the plasmid pCAMBIA1300 containing hygromycin resistance(HygR). Transgenic plants showing in vitro resistance to 50mg/L hygromycin were obtained using a selection procedure. Stable integration of argE and HPT genes into chromosomal DNA was proven by southern blot analysis and PCR analysis of genomic isolated from $T_0$ progenies. The fragments of 650 bp(HPT) were detected in transgenic rice lines. The 230 bp(argE) fragments were showed in agarose gel, and detected fragments were matched with size of argE specific primer. The microscopic feature of leaf on scanning electron microscope(SEM) revealed differences between clear and chalky in shape and arrangement of stoma but did not discriminate.

• Journal of Applied Biological Chemistry
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• v.58 no.3
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• pp.273-279
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• 2015

Phaeodactylum tricornutum is a model diatom that its genomic information and biological tools are well established. In this study, a gene encoding (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (PtHDR), a terminal enzyme of the methylerythritol phosphate pathway regulating chlorophyll and carotenoid biosynthesis, was isolated from P. tricornutum. The isolated gene was cloned into pPha-T1 vector containing fcpA promoter to prepare pPha-T1-HDR plasmid. As a positive control, pPha-T1-eGFP plasmid was constructed with egfp gene. Stable nuclear transformation was carried out with these plasmids by particle bombardment method and zeocin resistant colonies of P. tricornutum were selected on f/2 agar plate. In result, transformation efficiency was evaluated according to the amount of plasmid DNA coated with gold particles. Integration of introduced plasmids was confirmed with genomic DNA of each transformant by polymerase chain reaction. The eGFP fluorescence was visible in the cytoplasm, indicating that eGFP was successively expressed in P. tricornutum system. The transcript level of exogenous Pthdr gene was evaluated with the obtained transformants. The results presented here demonstrated that introduction of Pthdr gene into P. tricornutum chromosome succeeded and expression of PtHDR was enhanced under the fcpA promoter.

• Proceedings of the Korean Society of Tobacco Science Conference
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• 2001.05a
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• pp.72-78
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• 2001

농업 유전공학 기술은 생산성 향상, 환경보전, 식품의 안정성 및 품질향상에 기여함은 물론 농업의 경쟁력을 높일 수 있는 유일한 대안으로 인식되고 있다. 전 세계적으로 유전자 재조합 작물의 경작지는 2000년 한해동안 지난해 같은 기간에 비해 11% 증가하였으며 이는 1996년 대비 25배 증가하였고, 선진국과 개발도상국은 각각 2%, 51% 1999년 대비 증가하였다. 1983년 유전자 재조합에 의한 식물의 형질전환이 성공한 뒤 종묘업계는 형질전환 종자개발과 보급에 열중하고 있으며 종자시장에 형질전화 품종이 차지하는 비율은 2000년 30억 달러에서 2010년이면 전체의 60%인 200억 달러에 이를 것으로 전망된다. 1995년 제초제 저항성 콩(라운드업레디콩)이 농가에 보급된 이후 2000년 형질전환품종 재배면적이 3990만 ha에 이르렀고 1997년 미국과 캐나다는 옥수수, 대두, 면화, 감자, 유채 등의 형질전환 품종 재배로 각각 3억1400만 달러, 5300만 달러를 벌어들였음. 형질전환 품종의 보급 증가속도는 소비자들의 GMO에 대한 거부반응으로 다소 주춤한 상태이다. 그러나 최근 종자회사들은 생태계 위해성 논란을 피해갈 수 있는 연구로 이러한 상황을 돌파하려 하고 있다. 우리나라에서도 유전자변형 생물체에 관한 법률이 제정되었으며 많은 대학과 연구소에서 형질전환 연구가 꾸준히 이루어지고 있고 최근 제초제 저항성 벼와 바이러스 저항성 감자가 개발돼 GMO 안정성 점검에 들어가 있고, 살충성 배추, 혈압강하 토마토, 지방산 강화 들깨, 병저항성 고추 등도 실험실과 포장에서 재배되고 있다. 이르면 4-5년 뒤 형질전환 작물들이 농가에 보급될 전망이다. 이처럼 체크 툴은 Firewall의 수비능력을 보강하는 위치에 있다고 생각할 수 있다.다. 4 장에서는 3장에서 제기한 각각의 문제점에 대해 RAD 의 관점에 비추어 e-business 시스템의 단기개발을 실현하기 위한 고려사항이나 조건 해결책을 제안한다. 본 논문이 지금부터 e-business 를 시작하려고 하는 분, e-business 시스템의 개발을 시작하려고 하는 분께 단기간의 e-business 실현을 위한 하나의 지침이 된다면 다행이겠다.formable template is used to optimize the matching. Then, clustering the similar shapes by the distance between each centroid, papaya can be completely detected from the background.uage ("Association of research for algorithm of calculating machine (1992)"). As a result, conventional NN and CNN were available for interpolation of sampling data. Moreover, when nonlinear intensity is not so large under the field condition of small slope, interpolation performance of CNN was a little not so better than NN. However, when nonlinear intensity is large under the field condition of

• Journal of Life Science
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• v.25 no.10
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• pp.1164-1168
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• 2015

Previous research showed that chlorphenesin galactoside (CPN-Gal), a preservative in cosmetics, was safer than CPN against human skin cells [9]. To establish a stable and long-term process for CPN-Gal production, we investigated the repeated-batch process. In this process, β-gal-producing recombinant Escherichia coli cells were immobilized in calcium alginate beads, and CPN was converted to CPN-Gal by the transgalactosylation reaction. The process was conducted in a 300 ml flask, which contained E. coli cell-immobilized alginate beads, 33.8 mM of CPN, and 400 g/l of lactose. The pH and temperature were 7.0 and 40℃, respectively. During the repeated-batch operation, four consecutive batch operations were conducted successfully until 192 hr. The conversion yield of CPN to CPN-Gal was 64% during 192 hr, which was higher than the values in previous reports [3, 13]. Thereafter, however, the conversion yield gradually decreased until the operation was finished at 336 hr. Western blotting of immobilized E. coli cells revealed that β-gal gradually decreased after 192 hr. In addition, alginate beads were cracked when the operation was finished. It is probable that, including this loss of E. coli cells by cracks, deactivation, and product inhibition of E. coli β-gal might lead to a gradual decrease in the production of CPN-Gal after 192 hr. However, as the purification of β-gal is not necessary with β-gal-producing recombinant E. coli cells, β-gal-producing E. coli cells might be a practical and cost-effective approach for enzymatically synthesizing CPN-Gal. It is expected that this process will be extended to long-term production process of CPN-Gal for commercialization.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2014.10a
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• pp.131-134
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• 2014

The Internet allows firms to serve customers more effectively than ever before. In the B2C context, we examine the interrelationships among information privacy concerns, e-trust, and e-loyalty. The authors extend prior research by incorporating constructs of information privacy concerns into a more holistic conceptual framework. This study answers three research questions: Will the three components of information privacy concerns have a significant effect on e-loyalty through e-trust?, Does e-trust mediate e-loyalty?, Finally, do switching costs have a moderation effect between e-trust and e-loyalty?, The authors examine data from customers who have booked hotel accommodations online. The results support our hypotheses and confirm both the mediation role of e-trust and the moderation role of switching costs. Conceptually, this study provides an empirical validation of information privacy concerns, e-trust, and loyalty linkage. On the managerial level, this research provides insights into critical drivers of loyalty in the emerging online marketplace.

• The Journal of Society for e-Business Studies
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• v.18 no.3
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• pp.195-209
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• 2013

Considering the widespread use of social networking sites (SNSs) and the sudden shift of Filipino SNS usage from Friendster to Facebook, this paper examines five factors that affect user intentions to switch SNSs. By surveying switchers from Friendster to Facebook in the Philippines, satisfaction, alternative attractiveness, and social influence were significant factors affecting switching intent.