• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.5-5
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• 2017

Too little and too much water due to climatic events is a significant cause of global food insecurity. Crops are less productive under water-limited conditions and all major crops, with the exception of rice (Oryza sativa), die within a few days of complete submergence. To complement our studies on genes such as SUB1A, (an ERF-VII transcription factor that provides robust submergence tolerance) and AG1 (a TREHALOSE 6-P PHOSPHATASE that promotes establishment of young seedlings underwater), we have retooled INTACT (${\underline{I}}solation$ of ${\underline{N}}uclei$ ${\underline{TA}}gged$ in specific ${\underline{C}}ell$ ${\underline{T}}ypes$) and TRAP (${\underline{T}}ranslating$ ${\underline{R}}ibosome$ ${\underline{A}}ffinity$ ${\underline{P}}urification$) for rice. These technologies enable us to follow dynamics in chromatin, nuclear pre-mRNAs and ribosome-bound mRNAs in meristems and diverse cell types. With these technologies we can better interpret responses to stresses and reestablishment of homeostasis. These include stress acclimation strategies involving changes in metabolism and development, such as dynamics in suberin deposition in sub-epidermal layers of roots that limit water loss under drought and oxygen escape during waterlogging. Our new data uncover dynamic and reversible regulation at multiple levels of gene regulation and provide new insights into processes of stress resilience. Supported by US NSF-PGRP Plasticity (IOS-1238243), Secretome (IOS-1546879) and REU (DBI-146129) grants.

• Transactions of the Korean Society of Automotive Engineers
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• v.18 no.2
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• pp.31-38
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• 2010

Bus manufacturers have tested and studied the dynamic collapse behavior of a bus body structure in rollover since UN ECE established ECE Regulation 66 to provide the requirement for the strength of bus structure. In spite of the costly cycles of practical tests, however, it is still a hard task to meet the rollover regulation by means of local reinforcements in the bus structure. Therefore it is necessary to develop a well designed strategy for the rollover strength implemented in the early stage of vehicle development. In this study, the suitable development method for each design stage from a component to complete body structure was considered to make a well-established development process of a bus body structure for rollover safety. For the efficient approach of the concept design stage, a numerical model based on the plastic hinge theory was used instead of detailed shell models. After setting up the concept design for the component size and geometry, the shell model was used to confirm and optimize the whole structure composition. The process developed in this study was practically used as an effective method to predict the rollover behavior of a new bus body structure.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.6
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• pp.157-167
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• 2008

Through this study, the standard design guidelines for high speed train with power cars have been derived to meet the crashworthiness requirements of the Korean rollingstock safety regulation. The crashworthiness regulation requires some performance requirements for two heavy collision accident scenarios; a train-to-train collision at the relative speed of 36 kph, and a collision against a standard deformable obstacle of 15 ton at 110 kph. A standard high speed train composition was defined as 2PC-2ET-6T with 17ton axle load, similar to KTX-2 for the Honam express line. Using theoretical and numerical analyses, some crashworthy design guidelines were derived in terms of mean crush forces and energy absorptions for major crushable components. The derived design guidelines were evaluated and improved using one dimensional spring-mass dynamic simulation. It is shown from the simulation results that the suggested design guidelines can easily satisfy the domestic crashworthiness requirements.

• Molecules and Cells
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• v.27 no.1
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• pp.1-3
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• 2009

Mitotic spindle mediates the segregation of chromosomes in the cell cycle and the proper function of the spindle is crucial to the high fidelity of chromosome segregation and to the stability of the genome. Nucleation of microtubules (MTs) from centrosomes and chromatin represents two well-characterized pathways essential for the assembly of a dynamic spindle in mitosis. Recently, we identified a third MT nucleation pathway, in which existing MTs in the spindle act as a template to promote the nucleation and polymerization of MTs, thereby efficiently amplifying MTs in the spindle. We will review here our current understanding on the molecular mechanism, the physiological function and the cell-cycle regulation of MT amplification.

• Journal of Power Electronics
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• v.7 no.4
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• pp.310-317
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• 2007

In this paper, an analysis and hysteretic controller design of a $3^{rd}$ order buck converter is presented. The proposed hysteretic controller consists of an inner current-loop, just like the conventional cascade control scheme, and an outer voltage-loop for load voltage regulation. Although it is possible to include an inner current loop from different branches of the converter, from the feasibility and operational point of view, the load side capacitor current would be the better choice. The addition of an inner current-loop improves the dynamic performance of the converter while preserving the robustness of the hysteretic control. The controller formulation and closed-loop converter performance analysis are validated through computer simulations. Few experimental results of the proposed converter are given and compared with the buck converter.

• Journal of Power Electronics
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• v.10 no.1
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• pp.91-96
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• 2010

In this paper, a simplified control algorithm for a three-phase, four-wire unified power quality conditioner (UPQC) is presented to compensate for supply voltage distortions/unbalance, supply current harmonics, the supply neutral current, the reactive power and the load unbalance as well as to maintain zero voltage regulation (ZVR) at the point of common coupling (PCC). The UPQC is realized by the integration of series and shunt active filters (AFs) sharing a common dc bus capacitor. The shunt AF is realized using a three-phase, four leg voltage source inverter (VSI) and the series AF is realized using a three-phase, three leg VSI. A dynamic model of the UPQC is developed in the MATLAB/SIMULINK environment and the simulation results demonstrating the power quality improvement in the system are presented for different supply and load conditions.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.50-56
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• 2004

Bifurcation analysis of cell cycle regulation in the budding yeast is performed basedon the mathematical model by Chen et al [Molecular biology of cell, 11:369-391, 2000]. On the bifurcation diagram, locations of both stable and unstable solutions of the nonlinear differential equations are presented by taking the mass of cell as a controlparameter. Based on the bifurcation diagram, dynamic mechanism underlying the 'start' transition, initiation of a new round of cell cycle, and the 'finish' transition, completion of cell cycle and returning back to the initial state, is discussed: the 'start' transition is a transition from a stable fixed solution for a small mass and to an oscillatory state for a large mass, and the 'finish' transition is a switching back to the stable fixed solution from the oscillatory state. To understand the role of the genes during the cell cycle regulation, bifurcation diagrams for the mutants are compared with that of the wild type.

• BMB Reports
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• v.48 no.8
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• pp.429-430
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• 2015

Angiogenesis is a complex process involving dynamic interaction of various cell to cell interactions. Endothelial cell interactions regulated by growth factors, inflammatory cytokines, or hemodynamic stress are critical for balancing vascular quiescence and activation. Yes-associated protein (YAP), an effector of Hippo signaling, is known to play significant roles in maintaining cellular homeostasis. However, its role in endothelial cells for angiogenic regulation remains relatively unexplored. We demonstrated the critical role of YAP in vascular endothelial cells and elucidated the underlying molecular mechanisms involved in angiogenic regulation of YAP. YAP was expressed in active angiogenic regions where endothelial cell junctions were relatively loosened. Consistently, YAP subcellular localization and activity were regulated by VE-cadherin-mediated PI3K/Akt pathway. YAP thereby regulated endothelial sprouting via angiopoietin-2 expression. These results provide an insight into a model of coordinating endothelial junctional stability and angiogenic activation through YAP. [BMB Reports 2015; 48(8): 429-430]

• BMB Reports
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• v.51 no.6
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• pp.261-262
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• 2018

Aurora B is an important kinase involved in dynamic cellular events in mitosis. Aurora B activity is controlled by several post-translational modifications (PTMs). Among them, E3 ubiquitin ligase-mediated ubiquitination plays crucial roles in controlling the relocation and degradation of Aurora B. Aurora B, ubiquitinated by different E3 ligases, moves to the exact site for its mitotic function during metaphase-anaphase transition and is then degraded for cell cycle progression at the end of mitosis. However, how the stability of Aurora B is maintained until its degradation has been poorly understood. Recently, we have found that USP35 acts as a deubiquitinating enzyme (DUB) for Aurora B and affects its stability during cell division, thus being involved in the regulation of mitosis. In this review, we discuss the USP35-mediated deubiquitination of Aurora B and the regulation of mitotic progression by USP35.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.11a
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• pp.174-177
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• 2007

In this work, we researched static and dynamic characteristics concern to hydraulic regulator control process and parameter setting, which is used on liquid rocket engine regulation. The hydraulic regulator of 8K14 "SCUD" 9D21 engine is analyzed and on the basis of the developed mathematical modeling the analysis of response time and certification on automatic control accuracy is carried out. In this process, we find out specific design configuration of needle valve flow section that effects on pressure regulation performance.