• Journal of Korean Tunnelling and Underground Space Association
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• v.23 no.6
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• pp.423-438
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• 2021

The blade of motor grader is used for scattering and leveling the aggregates on the foundation of road construction site. The paper performed a design improvement research of the blade part to enhance the working efficiency of motor graders. The scattering works of aggregates by blade driving were simulated by DEM (discrete element method) of a dynamic code. The four design parameters were selected and a specific leveling scenario for the simulation was determined. The nine blade models were numerically experimented, and the sensitivity of each factors was analyzed. Next, the design factors that influence a blade performance have been selected by ANOVA, and these key design factors were applied to the progressive quadratic response surface method (PQRSM). The optimum set of design factors of the blade was finally proposed.

• Development and Reproduction
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• v.24 no.4
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• pp.249-261
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• 2020

Spermatogonial stem cells (SSCs) have stemness characteristics, including germ cell-specific imprints that allow them to form gametes. Spermatogenesis involves changes in gene expression such as a transition from expression of somatic to germ cell-specific genes, global repression of gene expression, meiotic sex chromosome inactivation, highly condensed packing of the nucleus with protamines, and morphogenesis. These step-by-step processes finally generate spermatozoa that are fertilization competent. Dynamic epigenetic modifications also confer totipotency to germ cells after fertilization. Primordial germ cells (PGCs) in embryos do not enter meiosis, remain in the proliferative stage, and are referred to as gonocytes, before entering quiescence. Gonocytes develop into SSCs at about 6 days after birth in rodents. Although chromatin structural modification by Polycomb is essential for gene silencing in mammals, and epigenetic changes are critical in spermatogenesis, a comprehensive understanding of transcriptional regulation is lacking. Recently, we evaluated the expression profiles of Yin Yang 1 (YY1) and CP2c in the gonads of E14.5 and 12-week-old mice. YY1 localizes at the nucleus and/or cytoplasm at specific stages of spermatogenesis, possibly by interaction with CP2c and YY1-interacting transcription factor. In the present article, we discuss the possible roles of YY1 and CP2c in spermatogenesis and stemness based on our results and a review of the relevant literature.

• Journal of the Korea institute for structural maintenance and inspection
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• v.25 no.6
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• pp.235-244
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• 2021

This study analyzes the applicability of limit state design method on bridge evaluation by considering the experiment of the existing bridge. The test strength of the member is obtained from the PSC beam bridge experiment. The test strength is compared with the calculated strength obtained from the statistical characteristics of material test strength and the two values are almost the same. The response modification factor and dynamic impact factor are obtained from the vehicle loading test. The rating factor is calculated by applying limit state design method as well as current evaluation method and the results are compared. The reliability index of the test bridge is calculated by using the statistical properties of the member strength obtained from material test and simulation. When the statistical properties of the PSC beam tested in this study are applied, the reliability index with a larger value was obtained than the reliability index obtained with the statistical properties of the design code.

• Annual Conference of KIPS
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• 2011.04a
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• pp.871-874
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• 2011

The fast emerging of network technology and the high demand of computing resources have prompted many organizations to outsource their storage and computing needs. Cloud based storage services such as Microsoft's Azure and Amazon's S3 allow customers to store and retrieve any amount of data, at anytime from anywhere via internet. The scalable and dynamic of the cloud storage services help their customer to reduce IT administration and maintenance costs. No doubt, cloud based storage services brought a lot of benefits to its customer by significantly reducing cost through optimization increased operating and economic efficiencies. However without appropriate security and privacy solution in place, it could become major issues to the organization. As data get produced, transferred and stored at off premise and multi tenant cloud based storage, it becomes vulnerable to unauthorized disclosure and unauthorized modification. An attacker able to change or modify data while data inflight or when data is stored on disk, so it is very important to secure data during its entire life-cycle. The traditional cryptography primitives for the purpose of data security protection cannot be directly adopted due to user's lose control of data under off premises cloud server. Secondly cloud based storage is not just a third party data warehouse, the data stored in cloud are frequently update by the users and lastly cloud computing is running in a simultaneous, cooperated and distributed manner. In our proposed mechanism we protect the integrity, authentication and confidentiality of cloud based data with the encrypt- then-upload concept. We modified and applied proxy re-encryption protocol in our proposed scheme. The whole process does not reveal the clear data to any third party including the cloud provider at any stage, this helps to make sure only the authorized user who own corresponding token able to access the data as well as preventing data from being shared without any permission from data owner. Besides, preventing the cloud storage providers from unauthorized access and making illegal authorization to access the data, our scheme also protect the data integrity by using hash function.

• Molecules and Cells
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• v.41 no.11
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• pp.953-963
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• 2018

The stepwise development of T cells from a multipotent precursor is guided by diverse mechanisms, including interactions among lineage-specific transcription factors (TFs) and epigenetic changes, such as DNA methylation and hydroxymethylation, which play crucial roles in mammalian development and lineage commitment. To elucidate the transcriptional networks and epigenetic mechanisms underlying T-cell lineage commitment, we investigated genome-wide changes in gene expression, DNA methylation and hydroxymethylation among populations representing five successive stages of T-cell development (DN3, DN4, DP, $CD4^+$, and $CD8^+$) by performing RNA-seq, MBD-seq and hMeDIP-seq, respectively. The most significant changes in the transcriptomes and epigenomes occurred during the DN4 to DP transition. During the DP stage, many genes involved in chromatin modification were up-regulated and exhibited dramatic changes in DNA hydroxymethylation. We also observed 436 alternative splicing events, and approximately 57% (252) of these events occurred during the DP stage. Many stage-specific, differentially methylated regions were observed near the stage-specific, differentially expressed genes. The dynamic changes in DNA methylation and hydroxymethylation were associated with the recruitment of stage-specific TFs. We elucidated interactive networks comprising TFs, chromatin modifiers, and DNA methylation and hope that this study provides a framework for the understanding of the molecular networks underlying T-cell lineage commitment.

• Journal of the Korean Society of Propulsion Engineers
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• v.15 no.2
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• pp.62-67
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• 2011

Launching tube is a kind of case to protect the guided weapons from external environments and conducted as a guide when they are fired. The guided weapons have been firmly kept at the launching tube and transferred, and would be separated at the required time when they are fired To meet the aim, it has been used explosive bolts which are reliable and efficient mechanical fastening devices having the special feature of a built-in release. The disadvantage of explosive bolt lies in that it is based on the high explosive effect of a pyrotechnic charge. When the explosive bolt is ignited, there are some bad effects; a flame, fragments and pyro-shock. Because of these bad effects there are many restriction to use bolt as joining devices to precision guided weapons. To solve these problems, the aim of the present work is to invent the ball-type separation bolt which is a pyrotechnically releasable mechanical linking device for two mechanical elements that does not suffer from such drawbacks. A standard pressure cartridge can moreover be easily integrated inside the device according to the present work and this with no modification to its structure. The present work was represented quantitatively the margin of separation safety and analysed separation mechanism in ball type separating bolt to perform the dynamic separation test.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1992.10a
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• pp.59-64
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• 1992

터빈 블레이드와 같이 회전하는 구조물의 파단은 공진 근처에서 진동이 발 생할 때에 이에 기인하는 피로에 의하여 발생한다. 그러므로 이와 같은 파단 을 피하기 위해서는 설계 단계에서 이론적인 계산에 의하여 구조물의 고유 진동수를 결정하는 것이 상당히 중요하다. 판이 회전을 받게 되면 원심력에 의하여 판의 강성이 증가하므로 고유진동수가 회전하지 않는 판의 고유진동 수보다는 상당히 증가하게 된다. 이에 대한 연구가 국내외에서 상당수 행하 여졌지만, 연구의 대부분이 회전의 영향을 고려하지 않은 정지판(stationary plate)에 대한 것이며 뢰전을 고려한 연구는 극히 제한되어 있다. 또한 회전 의 영향을 고려한 연구의 대부분이 해석 대상을 보로서 단순화 시켰고 해법 으로는 유한요소법과 Ritz법 등을 사용하였다. 이는 블레이드가 지니고 있는 기하학적인 형상과 진동 특성이 해석적인 방법으로 해결하는 데에는 상당한 어려움이 있기 때문이다. 실제적으로는 터빈 블레이드와 같은 회전체의 진동 특성이 설치각이나 비틀림각, 판의 형상비, 회전속도 등의 변화에 의하여 영 향을 받기 때문에 보와 같은 진동 거동을 보이기보다는 판이나 셀과 같은 진동 거동을 보이므로 보다 정확한 해석을 수행하기 위해서는 해석 대상을 판이나 셀로서 취급하는 것이 타당하다. 따라서 본 연구에서는 위와 같은 이 유 때문에 해석 대상을 등방성 사각판과 직교이방성 복합재료 사각판으로 선택하였으며, 구조물의 고유진동수에 영향을 미치는 다음과 같은 인자들을 해석에 고려하였다. 1. 회전속도 (rotational speed) 2. 설치각 (setting angle) 3. 허브의 반경 (hub radius) 4. 판의 형상비 (aspect ratio) 5. 적층순서 (stacking sequence)구조물에 대한 동적실험(dynamic test)을 통하여 단기간에 동적특성을 결정하고 SDM(structure dynamic modification)이나 FRS(force response simulation)를 수행하여 임의의 좌표 공간에 대한 진동수준을 해석적으로 예측할 뿐만 아니라 구조물의 진동제어 를 위한 동적인자를 변경시킬 수 있는 정보를 제공하며 장비를 방진할 경우 신뢰성 있는 전달률을 결정할 수 있다. 실험적으로 철교, 교량이나 건물의 철골구조 및 2층 바닥 등 대,중형의 복잡한 구조물에 대항 동특성을 나타내 는 모빌리티를 결정할 경우 충격 가진 실험이 사용되는 실험장비 측면에서 나 실험을 수행하는 과정이 대체적으로 간편하다. 그러나 이 경우 대상 구조 물을 충분히 가진시킬수 있는 용량의 대형 충격기(large impact hammer)가 필요하게 된다. 이러한 동적실험은 약 길이 61m, 폭 16m의 4경간 교량에 대 하여 동적실험을 수행하여 가능성을 확인하였다. 여기서는 실험실 수준의 평 판모델을 제작하고 실제 현장에서 이루어질 수 있는 진동제어 구조물에 대 한 동적실험 및 FRS를 수행하는 과정과 동일하게 따름으로써 실제 발생할 수 있는 오차나 error를 실험실내의 차원에서 파악하여 진동원을 있는 구조 물에 대한 진동제어기술을 보유하고자 한다. 이용한 해마의 부피측정은 해마경화증 환자의 진단에 있어 육안적인 MR 진단이 어려운 제한된 경우에만 실제적 도움을 줄 수 있는 보조적인 방법으로 생각된다.ofile whereas relaxivity at high field is not affected by τS. On the other hand, the change in τV does not affect low field profile but stron

• Journal of the Korea Society of Computer and Information
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• v.16 no.3
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• pp.109-119
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• 2011

The virtual reality (VR) technology has been used as the application of architectural presentation or simulation tool in the field of industry. The high immersion and intuitive visual information are the great merits of design evaluation or environmental simulation when we are using the virtual environments. But the distortion of distance perception in VR is still a big problem when the accuracy of distance presentation is strictly required. For example, distance estimation is especially important when the virtual environments are applied to the presentational tool for evaluation the space design or planning in the field of architecture. If there are some perception error between the built space in real and represented space in virtual, the accurate design evaluation or modification of design is hard to be carried out during the design development stage. In this paper, we have carried out some experiments about distance estimation in the immersive virtual environments to verify the factors and their influence. We made a hypothesis that the lack of the information for the user in VR causes the different distance estimation from the real world because users are usually comfortable with moving fast and long distance in VR environments compared with moving slow and short distance in real space. So, we carried out basic experiment to prove our hypothesis that the lack of information makes subjects estimate the distance of walking in VR shorter compared with the same distance in real. Also, among the factors that probably affect the distance estimation in VR, we have verified the influence of the image resolution. The influence of resolution degradation of image on the distance estimation was verified with the condition of static and dynamic images. The results showed that the resolution has deep relation with the distance estimation. For example, the subject underestimated the distance at the lower resolution condition. We also found the methods of the making the lower resolution image could affect on the visual perception of subjects.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.1-11
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• 2016

The effect of the migration of nanoparticles near the wall of a channel on the convective heat transfer in a laminar flow of $SiO_2$ nanoparticle suspensions (nanofluids) under constant wall heat flux boundary conditions was numerically and experimentally investigated in this study. The dynamic thermal conductivity of the aqueous $SiO_2$ nanofluids was measured using T-type thermocouples attached to the outer surface of a stainless steel circular tube (with a length of 1 m and diameter of 1.75 mm). The nanofluids used in this study were synthesized by dispersing $SiO_2$ spherical nanoparticles with a diameter of 24 nm in de-ionized water (DIW). The enhancement of the thermal conductivity of the nanofluids (e.g., an increase of up to 7.9 %) was demonstrated by comparing the temperature profiles in the flow of the nanofluids with that in the flow of the basefluids (i.e., DIW). However, this trend was not demonstrated in the computational analysis, because the numerical models were based on continuum assumptions and flow features involving nanoparticles in a stable colloidal solution. Thus, to explore the non-continuum effects, such as the modification of the morphology caused by nanoparticle-wall interactions on the heat exchanging surfaces (e.g., the isolated and dispersed precipitation of the nanoparticles), additional experiments were performed using DIW right after the measurements using the nanofluids.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.6A
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• pp.535-542
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• 2010

Precise estimation of a structure's dynamic characteristics is indispensable for ensuring stable dynamic responses during lifetime especially for the structures which can experience resonance such as railway bridges. In this paper, the results of forced vibration tests of different excitation methods (vibration exciter and impact hammer) are compared to examine the differences and the cause of differences of extracted natural frequencies. Consequently a natural frequency modification method is suggested to eliminate effects of non-structural disturbance factors. Also, sequential forced vibration tests are performed before and after track construction according to the construction stage of a railway bridge, and the variation of natural frequencies are examined. Effect of added mass of vibration exciter and variation of support condition due to the level of excitation force are concluded as the major cause of natural frequency differences. Thus eliminating these effects can enhance the reliability of the extracted natural frequencies. Construction of track affects not only the mass of structure but also the stiffness of the structure. Also, the amount of increase in stiffness varies according to the level of structural deflection. Therefore, reasonable estimation of the level of structural response during operation is important for precise natural frequency calculation at design phase.