• Asian Pacific Journal of Cancer Prevention
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• v.13 no.8
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• pp.3583-3587
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• 2012

SUMOylation has emerged as an important post-translational modification that modulates the localization, stability and activity of a broad spectrum of proteins. A dynamic process, it can be reversed by a family of SUMO-specific proteases (SENPs). However, the biological roles of SENPs in mammalian development and pathogenesis remain largely elusive. Here, we demonstrated that SENP2 plays a critical role in the control of hepatocellular carcinoma cell growth. SENP2 was found to be down-regulated in hepatocellular carcinoma (HCC) tissues and over-expression suppressed the growth and colony formation of HCC cells. In contrast, silencing of SENP2 by siRNAs promoted cancer cell growth. We further found that stability of ${\beta}$-catenin was markedly decreased when SENP2 was over-expressed. Interestingly, the decrease was dependent on the de-SUMOylation activity of SENP2, because over-expression of a SENP2 catalytic mutant form had no obviously effects on ${\beta}$-catenin. Our results suggest that SENP2 might play a role in hepatocellular carcinoma cell growth control by modulating the stability of ${\beta}$-catenin.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2001.11a
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• pp.155-160
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• 2001

Actual engineering structure is frequently very complex, and parts of structure are designed independently by different engineers. Also each structure contains so many degree of freedom. For these reason, methods have been developed which permits the structure to be divided into components or substructures, with analysis being done on a small substructure in order to obtain a full structural system. In such case, because of different mesh size among finite element model (FEM) or different matching points among FEM models and experimentally obtained models, their interfacing points may be non-matching. Solving this non-matching problem is useful to other application such as structural dynamic modification or model updating. In this work, virtual node concept is introduced. Lagrange multipliers are used to enforce the interface compatibility constraint, and interface displacement is approximated by polynomial presentation. The governing equation of whole structure is derived using hybrid variational principle. The eigenvalue of whole structure are calculated using the determinant search method. The number of degree of freedom in the eigenvalue problem can be drastically reduced to just the number of interface degree of freedom. Some numerical simulation is performed to show usefulness of synthesis method.

• Journal of Ocean Engineering and Technology
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• v.24 no.2
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• pp.25-33
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• 2010

Collision between vessels may lead to structural damage and penetration of hulls. The structural damage of a hull may eventually bring about global collapse of the hull girder and outflow of oil, which would contaminate seawater. Therefore, various regulations require the strength of a vessel after collision to satisfy given criteria, and owners usually request collision analyses to confirm the structural safety of their vessels. In the process of designing a vessel to satisfy the collision strength criteria, the strength has been assessed mostly by conducting collision analyses using numerical techniques, such as dynamic, non-linear, finite-element analysis. Design is an inherently iterative process during which many changes are necessary due to the endless needs for reinforcement and modification. Numerical techniques are not adequate for coping with a situation in which collision analysis is frequently required to provide the revised results that reflect the repetitive changes in designs. Numerical techniques require a lot of time and money to conduct in spite of recent improvements in computing power and in the productivity of modeling tools. Therefore, in this paper, an analytical technique is introduced and a collision problem is idealized and simplified using reasonable assumptions based on appropriate background. The technique was applied to an example of an actual FPSO and verified by comparing the results with results from the numerical technique. A good correlation was apparent between the results of the analytical and numerical techniques.

• Advances in concrete construction
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• v.9 no.4
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• pp.423-435
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• 2020

Open ground story (OGS) reinforced concrete (RC) buildings are vulnerable to the complete collapse or severe damages under seismic actions. This study investigates the effectiveness of four different strengthening techniques representing the local and global modifications to improve the seismic performance of a non-ductile RC OGS frame. Steel caging and concrete jacketing methods of column strengthening are considered as the local modification techniques, whereas steel bracing and RC shear wall systems are selected as the global strengthening techniques in this study. Performance-based plastic design (PBPD) approach relying on energy-balance concept has been adopted to determine the required design force demand on the strengthening elements. Nonlinear static and dynamic analyses are carried out on the numerical models of study frames to assess the effectiveness of selected strengthening techniques in improving the seismic performance of OGS frame.. Strengthening techniques based on steel braces and RC shear wall significantly reduced the peak interstory drift response of the OGS frame. However, the peak floor acceleration of these strengthened frames is amplified by more than 2.5 times as compared to that of unstrengthened frame. Steel caging technique of column strengthening resulted in a reasonable reduction in the peak interstory drift response without substantial amplification in peak floor acceleration of the OSG frame.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.4
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• pp.25-30
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• 2012

The transportation is almost dependent on a single fuel petroleum with transportation energy dilemma. Hybrid Electric Vehicle(HEV) technology holds more advantages on efficiency improvements for petroleum consumption at the transportation. And bearing is recognized as the important component of gearbox. Gearboxes for HEV transmission have been ensured the highest reliability over some years in withstanding high dynamic loads. At the same time, the demands of lightweight design and cost minimization are required by thought-out design, high-quality material, superior production quality and maintenance. In order to design a reliable and lightweight gearbox, it is necessary to analyze bearing rating life methods between standard and different bearing companies with calculation methods for modification factors. In this paper, the influence of life time of bearings will be pointed out. Bearing contact stress and load stress distribution of HEV gearbox are obtained and compared with Romaxdesigner and BearinX. And the unequal wear of the left bearing for the gearbox intermediate shaft is investigated between simulation and test.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.11 no.2
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• pp.144-151
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• 2012

Polishing is not only one of the most frequently adopted processes in modern industries, but also the most critical one to the surface quality of the products such as semi conductor wafers and LED sapphire wafers. With the required specifications for the wafer surface quality getting more and more strengthened, the manufacturers are spending huge amount of cost to renew the machine to meet the enhanced surface specifications. Surface qualities of the wafers are mostly damaged by the structural vibrations of the polishing machines. In this paper, the dynamic characteristics of a wafer polishing machine have been analyzed through the frequency response test and the computer simulation. And the supporting structure of a polishing machine has been investigated to minimize the vibration transmissions, to improve the stability of the machine and further to reduce the defects of the polished products. The result of the study shows that simple design modifications of the supporting structure without altering the main structure of the machine can substantially suppress the vibrations of the machine with negligible expenses.

• Journal of the Korean Society of Clothing and Textiles
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• v.19 no.2
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• pp.190-202
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• 1995

The Optical Art is based on the principle of visual perception of the illusionary effects which induce psychological responses. It has influenced greatly on the Texile Design in that unique iJlusionary creativity of pattern simulates the visual sense of special movement; the dynamic psylosophy of vitalism. The Optical pattern has become a highly valued item due to its innovative effect in aesthetic direction. According to Vitor Vasarely the pioneer in this area, the integration and the inseparability of form and color which he calls 'Plastic Unity' provides the basis for the composition of infinite variety. The composition of infinite variety. The composition reveals the complex interaction between the space and form relating to order, repetition, combination and permutation. It is not simple to create optical patterns due to the extreme complexity composed by the multi-dimension and the infusion of form and color giving immensely varied movement. The purposes of this study are as follows; 1) to classify the complex processes of optical pattern on the basis of formative method. 2) to develop creative ideas for progressive contemporary textile design In this study, the analysis of applied methods is concentrated, which is based 1) on the gradual modification and on the transformation of the basic plastic elements which depend on thle direction of visual points involVing contradictory perspectives 2) on the composition varied special situations by repeating, overlapping and converging a series of idetUical units or by means of irrdiation, radiation and etc.

• Journal of Korean Society for Atmospheric Environment
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• v.27 no.6
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• pp.681-691
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• 2011

The size distributions of smoke particles from fire are prerequisite for the studies on fire detection and adverse health effects. Above the flame of the fire, coagulation dominates and the smoke particles grow from 1 to 50 nm up to 100 to 3,000 nm, sizes ranging from the free-molecular regime to the continuum regime. The characteristics of the agglomeration of the smoke particles are well known, independently for each of the free-molecular and continuum regimes. However, there are not many systematic studies in the entire regime by the complexity of the mechanisms. The purpose of this work is to find the characteristics of the development of the size distribution of smoke particles by agglomeration in the entire size range covering the free-molecular regime, via transition regime, to the near-continuum and continuum regime for each variation of parameters such as fractal dimension, primary particle size and dimensionless coagulation time. In this work, the dynamic equation for the discrete-size spectrum of the particles was solved using a nodal method based on the modification of a sectional method. In the calculation, the collision frequency function for the entire regime, which is derived by using the concept of collision volume and general enhancement function, was applied. The self-preserving size distribution for the entire regime is compared with the ones for the free-molecular or continuum regimes for each variation of the parameters.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.122-125
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• 2006

The Substructure Synthesis means the technology to predict the dynamic properties of an assembly from the properties of its components, or to predict the effect of a modification on a structure. The FRF Based Substructuring method is a kind of the Substructure Synthesis and very useful to predict the efficiency of the product in the early stage of development. Especially, the Hybrid FBS method is very useful to predict the vehicle NVH characteristics after modifying some components of the vehicle. Target components can be established on the basis of test models and FE models of the prototype constructed in the early stage of development. In this study, the Hybrid FBS method was applied to vehicle subframe and car-body in order to reduce vehicle interior noise induced by engine exciting force.

• Smart Structures and Systems
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• v.7 no.3
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• pp.213-222
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• 2011

The native extracellular matrix (ECM) consists of an integrated fibrous protein network and proteoglycan-based ground (hydrogel) substance. We designed a novel electrospinning technique to engineer a three dimensional fiber-hydrogel composite that mimics the native ECM structure, is injectable, and has practical macroscale dimensions for clinically relevant tissue defects. In a model system of articular cartilage tissue engineering, the fiber-hydrogel composites enhanced the biological response of adult stem cells, with dynamic mechanical stimulation resulting in near native levels of extracellular matrix. This technology platform was expanded through structural and biochemical modification of the fibers including hydrophilic fibers containing chondroitin sulfate, a significant component of endogenous tissues, and hydrophobic fibers containing ECM microparticles.