• Microbiology and Biotechnology Letters
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• v.46 no.1
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• pp.29-39
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• 2018

Vibrio vulnificus needs various responsive mechanisms to survive and transmit successfully in alternative niches of human and marine environments, and to ensure the acquisition of steady energy supply to facilitate such unique life style. The bacterium had genetic constitution very different from that of Escherichia coli regarding metabolism of glycogen, a major energy reserve. V. vulnificus accumulated more glycogen than other bacteria and at various levels according to culture medium and carbon source supplied in excess. Glycogen was accumulated to the highest level in Luria-Bertani (3.08 mg/mg protein) and heart infusion (4.30 mg/mg protein) complex media supplemented with 1% (w/v) maltodextrin at 3 h into the stationary phase. Regarding effect of carbon source, more glycogen was accumulated when maltodextrin (2.34 mg/mg protein) was added than when glucose or maltose (0.78.1-14 mg/mg protein) was added as an excessive carbon source to M9 minimal medium, suggesting that maltodextrin metabolism might affect glycogen metabolism very closely. These results were supported by the analysis using the malP (encoding a maltodextrin phosphorylase) and malQ (encoding a 4-${\alpha}$-glucanotransferase) mutants, which accumulated much less glycogen than wild type when either glucose or maltodextrin was supplied as an excessive carbon source, but at different levels (3.1-80.3% of wild type glycogen). Therefore, multiple pathways for glycogen metabolism were likely to function in V. vulnificus and that responding to maltodextrin might be more efficient in synthesizing glycogen. All of the glycogen samples from 3 V. vulnificus strains under various conditions showed a narrow side chain length distribution with short chains (G4-G6) as major ones. Not only the comparatively large accumulation volume but also the structure of glycogen in V. vulnificus, compared to other bacteria, may explain durability of the bacterium in external environment.

• Transactions of the Korean Society of Automotive Engineers
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• v.24 no.3
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• pp.338-344
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• 2016

The study is actively being performed to increase fuel injection pressure of common rail system among countermeasures to meet the emission regulation strengthen of the Diesel engine. The common rail fuel injection tube in such ultra high pressure common rail system has the weakest structural characteristics against vibration that is generated by fuel injection pressure and pulsation during engine operation and driving. Thus the extreme durability is required for common rail fuel injection tube, and the drawing process is being magnified as the most important technical fact for strength of seamless pipe that is the raw material of common rail tube. In this respect, we analyzed the characteristic of dimension and stress variation of the ultra high pressure common rail fuel injection tube by variation of Die and Plug angle in drawing process. Based on the analysis, we tried to obtain the raw material strength of common rail fuel injection tube for applying to the ultra high pressure common rail system. As a result, Plug angle is more important than entry angle of Die and we could obtain the target dimension and strength of the ultra high pressure common rail fuel injection tube through optimization of Plug angle.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.1
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• pp.8-13
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• 2008

Emissions from automobiles have long been considered a prime source of pollutants involved in smog formation and ozone production. Especially VOCs are associated with serious environmental problems such as photo-chemical smog as well as human health effects. Since motor vehicles are a major source of VOCs, estimating of emission from mobile source is the most important factor to control VOCs. VOCs are emitted from various pollution like motor vehicles, mobile and stationary source that has characteristics of toxicity, cancer-causing, bio-accumulation, durability in air and diffusion can exert a bad influence upon human health and environment. However we don't have any standard or regulation about VOCs emissions. This study is summarized as VOCs emission characteristics from in-use light-duty diesel and LPG fueled vehicles. The vehicle exhaust-gas test mode is CVS cycle and nier-10 cycles that developed on EPA and National Institute of Environmental Research. TO-14 method (Toxic Organic) was chosen for VOCs analysis from EPA in USA. This study results will be useful when make a emission factor and rule making of emission standard about domestic VOCs emission for the improve to air condition.

• Journal of Conservation Science
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• v.18 s.18
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• pp.33-50
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• 2006

Around Yeongsan river basin, there are Yeongkwang Gundong, Muan Inpyeong Tombs, Muu Gusan-ri Tombs and Hampyeong Guksan remain from which a lot of iron artifacts were excavated. Among them, 6 iron artifacts were chosen, and their microstructures were analyzed. As a result, Iron artifacts were produced sponge iron by the low temperature reduction process and a part of microstructure have the possibility that steel made by decarburizing. And also, by tempering the parts which need high strength, the iron artifacts had high strength and by distributing the weakness of the tempered structure to the nearby untempered parts, their breaking was prevented and they had the durability. These skills were used then. Especially these skills were found to be used in the 2nd century by high skilled people because an iron axe excavated at Yeongkwang Gundong of 2nd century by the historical record showed that the skill was used. Also microstructures were found to show the possibility that the iron technology was inherited to the late 5th century. When producing iron artifacts made of sponge iron containing small amount of carbon, that was made by the production process repeating molding, carburizing, heat treatment and hammering.

• Journal of Microbiology and Biotechnology
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• v.25 no.3
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• pp.399-406
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• 2015

In this study, we developed a composite filler comprising cross-linked hyaluronic acid (HA) and human collagen (COL) derived from the human umbilical cord with the aim of improving its biocompatibility and longevity compared with commercially available fillers. After HA/COL composite fillers were made in two different ratios (10:1 and 5:1), the physical properties of the fillers were evaluated. The interior morphologies and in vivo weight change of these hydrogels were also characterized at 1-16 weeks after injection into mice. To evaluate their biocompatibility and durability in vivo, we injected the composite fillers into nude mice subcutaneously. The variations of injected gel weight were measured and compared with the commercial dermal fillers (Restylane and TheraFill). The composites showed improved or similar physical properties (complex viscosity of 19-22 × 10⁵ cP, and injection force of 10-12 N) over the commercial dermal fillers. Sixteen weeks following the injection, the ratio of remaining composite filler weight to initial weight (75.5 ± 16.9%; 10:1) was shown to be greater than that of the commercial fillers (43.2 ± 8.1%, Restylane; 12.3 ± 5.3%, TheraFill). In addition, immunohistochemical analysis with angiogenesis-related markers such as isolectin and vWF revealed newly formed blood vessels and cellular influx into the composite filler, which were not observed in the other fillers. These results clearly suggest that the HA/COL composite filler is a superior candidate for soft tissue reconstruction. The filler we developed may be a suitable candidate as an injectable dermal filler for tissue augmentation in humans.

• The Journal of Asian Finance, Economics and Business
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• v.4 no.2
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• pp.75-80
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• 2017

Hand phones are standard paraphernalia among university students. Factors that motivate them to own the gadget would be of interest to both the students as well as marketers. Hand phone usage is an unexamined field in academic literature, this exploratory study attempts to investigate student purchasing motives in cellular phone markets. It also intends to know the student's satisfaction with the different services and its future impact on socio economic changes. In this study, undergraduates (n=336) were requested to specify their purchase criteria of hand phone. The instrument used in the study to collect feedback from the respondents contains a combination of open-ended and scaled questions, and some background demographics. The study employed content analysis, Pearson's correlation, and t-tests as the primary tools to analyze the responses. Results show that brand was rated as the most important factor in student purchase decisions. Other factors, arranged in decreasing order of importance comprise price, product quality, features, durability, availability, promotion, and post purchase service. Brand and price correlated significantly. It is also observed that there is very little difference regarding preference between brand and price in purchasing a hand phone. Marketers may formulate suitable strategies out of the findings to promote hand phones to university undergraduates in Malaysia by emphasizing at brands and price.

• The Journal of the Korea Contents Association
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• v.15 no.11
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• pp.137-153
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• 2015

In this study, volunteer experience(motive, durability, satisfaction, content) was set up as an independent variable, career maturity degree(determinacy, involvement, independence, tendency) was set up as a dependent variable, and the average difference and effect of the sub factors for each of them were reviewed. For this purpose, questionnaires were performed with 519 students at the middle schools and high schools in Busan. With 376 students having volunteer experiences among them, an analysis was done to find out the effect of volunteer experience on career maturity. The results of this study are as follows: First, whether having volunteer experience or not affected the career maturity among youths. That is the group with volunteer experience had higher level of interest, involvement and value in career and jobs than the group without them. Second, it showed that volunteer experience affected career maturity among youths. Specifically, career maturity was higher as their motive for volunteer activities was selfish, their satisfaction for volunteer activities was higher and they had more volunteer activities.

• Journal of the Korean Society for Railway
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• v.12 no.6
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• pp.1024-1029
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• 2009

According to increase of massive concrete structures, Thermal crack done by heat of hydration have been increased in a process of the execution. In case of a subway concrete box structure, thickness of structures are almost over 1 meter, and penetrating crack by external restraint takes a bad effect on the durability with a water leakage and deterioration in especially concrete wall. A try of shortening a constructing period often occurs as subway is generally constructed according to a road to decrease a traffic jam. The research proposes executing method by skipping one block as a plan of shortening a constructing period and investigate an influence on thermal cracking control. Additionally, sensitivity analysis is accomplished by changing a height of a concrete wall, and concrete placing and air temperature met with a field condition.

• Journal of the Korean Solar Energy Society
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• v.36 no.2
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• pp.65-72
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• 2016

PV module is installed in various outdoor conditions such as solar irradiation, ambient temperature, wind speed and etc. Increase in solar cell temperature within PV module aggravates the behaviour and durability of PV module. It is difficult to measure temperature among respective PV module components during PV module operating, because the temperature within PV module depends on thermal characteristics of PV module components materials as well as operating conditions such as irradiation, outdoor temperature, wind etc. In this paper, simulation by using finite element method is conducted to predict the temperature of each components within PV module installed to outdoor circumstance. PV module structure based on conventional crystalline Si module is designed and the measured values of thickness and thermal parameters of component materials are used. The validation of simulation model is confirmed by comparing the calculated results with the measured temperatures data of PV module. The simulation model is also applied to estimate the thermal radiation of PV module by front glass and back sheet.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.27 no.5
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• pp.385-392
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• 2014

The isolators have been generally used to reduce a seismic force. If the isolators apply to the nuclear power plants(NPPs), the durability and capacity for the structures and equipments should be ensured during the life time. In this study, the long-term behavior of isolated NPPs was analyzed for ensuring the seismic safety. The properties of isolator due to the age-related degradation were analyzed. And the seismic behavior of isolated buildings was analyzed by considering the aging of rubber bearings in different temperature environments. According to the analysis results, the natural frequency of structures was increased with time. But the maximum acceleration and maximum displacement of isolated structures have not changed significantly. Although the damaged of structure did not occurred by aging of isolators, it was presented that the spectral acceleration at the target frequency of isolated structure increased with the temperature. Therefore the isolators in the isolated buildings should be carefully designed and manufactured considering the temperature-dependancy of rubber material.