• Journal of The Korean Society of Grassland and Forage Science
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• v.31 no.3
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• pp.269-276
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• 2011

To investigate the physiological effects of mycorrhizal colonization on drought stress tolerance in white clover, the responses of leaf water potential (${\Psi}_{PL}$), relative water content (RWC), leaf dry mass, photosynthesis rate, transpiration, stomatal conductance, proline and ammonia were assessed periodically during 7 days in non-AM and AM plants under wellwatered or drought-stressed conditions. Under well-watered conditions, the examined parameters were not significantly changed or very little affected by AM symbiosis. Drought decreased water potential, relative water content, photosynthesis rate, transpiration and stomatal con ductance by 68.6%, 22.7%, 97.7%, 83.9% and 84.9%, respectively, in non-AM plants, meanwhile 46.8%, 13.4%, 50.3%, 44.8% and 54.7%, respectively, in AM plants. In addition, drought increased ammonia and proline by 31.8% and 162%, respectively, in non-AM plants, while 20.9% and 76.9%, respectively, in AM plants. These results clearly showed that mycorrhizal colonization significantly relieved the responses of physiological parameters to drought stress in white clover.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.13 no.8
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• pp.3719-3725
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• 2012

Due to the recent shortage of well-graded river sand resulting from a rapid growth of concrete construction, sea sand, crushed sand, and etc. are increasingly used instead. It is, however, well noted that non-washed sea sand leads to corrosion of the reinforcing steel in concrete, and thus eventually results in damage to concrete. Also, the crushed sand is not being widely used, since it is difficult to maintain the allowable amount of passing 0.08mm sieve and to adjust grading. On the other hand, because the fine sand of Nakdong-River has a poor grading but good quality as a fine aggregate for concrete, it is strongly needed to investigate the fine sand as an alternative fine aggregate. Thus, the purpose of this research is to evaluate the physical properties of the fine sand of Nakdong-River to utilize it actively as a fine aggregate. For this purpose, after the sand samples were collected according to typical location of main stream of Nakdong-River, the physical properties such as density in oven-dry condition, grading, unit volume mass, and etc. of them were estimated. It was observed from the test results that physical properties of the fine sand of Nakdong-River except grading were found to be excellent.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.620-624
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• 2009

"Egreen" rye (Secale cereale L.) was developed by National Institute of Crop Science (NICS), RDA. It was released in 2007 because of its excellent earliness and yield potential. Crossing was made by an open pollination with 10 rye lines in 1995. Many lines from the open pollinated population were handled in a mass selection program in subsequent generations. Among them the line 'SR95POP-S1-116-1-1-2'was selected for earliness and promising agronomic appearance and placed in yield trial for two years from 2003 to 2004. In 2005, it was designated "Homil 34" and subjected to regional yield trials. Over 3 years, "Egreen" averaged 8.34 MT ha-1 of forage yield (based on dry matter) harvested at the heading date of check variety "Olhomil", which was superior to other varieties such as "Olhomil" and "Koolgrazer" with an increase of 8% and 10%, respectively. Heading date of "Egreen" was April 22, 3 days earlier than that of "Olhomil". It would be recommended as a winter crop forforage and green manure before planting either maize or rice in Korea.

• Proceedings of KOSOMES biannual meeting
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• 2008.05a
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• pp.185-189
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• 2008

This study was conducted to find out the effects of relationship between ocean conditions and snowfall when cold and dry continental air mass passes through the West Sea of Korea. Route of continental high atmospheric pressure can cause effect on snowfall at the west regions (Inchoen, Gunsan, Mokpo) of the Korean Peninsula. The continental high atmospheric pressure extend from the southern China to western coastal region of the Korean Peninsula during the December, and it extend from the north side of China through Bohai Sea and Yodong Peninsula to central area of the Korean Peninsula during the February. Therefore, more snowfall recorded in Incheon is higher during Feb. than Dec.. whereas Gunsan and Mokpo is the opposite. The heavy snowfall at the western coastal region of Korea was caused by loss of the heat from the ocean to air when it's higher than $100W/m^2$. the heavy snowfall was also observed when the arrangement of continental high atmospheric pressure and low pressure was high at the West and low at the East, which formed a front in West and when the wind blow from the North or North West at the speed of $4\sim8m/sec$. There were not much relation between salinity in the western sea and snowfall in the western coastal region of Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.3
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• pp.175-181
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• 2000

This study was carried out to investigate the change of soil physical properties in long-term fertilized paddy soils with a Fine silty family of Typic Halpaqueps (Pyeongtaeg series). Treatments fertilized consisted of no fertilizer, compost, NPK, NPK+compost for thirty one years and of NPK+silicate for seventeen years. Water stable aggregate and degree of aggregate stability, which were higher in surface-soil than sub-soil, were high in order of NPK + compost > NPK + silicate > compost > NPK > no fertilizer plot. The ratio of aggregate larger than 0.5mm was high at compost and silicate plots but that smaller than 0.5mm was high at no fertilizer and NPK plots. And this aggregate stability showed negative correlation with soil hardness and bulk density ; positive correlation with sedimentation volume of soils in water. Sedimentation volume of soils in water was a little higher in surface-soil than sub-soil and in wet soil than dry soil, respectively. Pore space ratio and water retention capacity of soils were the most increased by the application of compost and not affected by silicate as in cases of liquid limit and plastic limit. Ignition loss of soils was high in order of NPK + compost > compost > NPK + silicate > NPK > no fertilizer plot. And field shattering ratio of soil mass smaller than 25.4mm was relatively high in NPK + compost, compost, and silicate plots.

• Journal of Applied Biological Chemistry
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• v.64 no.3
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• pp.217-223
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• 2021

5-aminolevulinic acid (ALA) is a representative photosensitizer used in numerous fields including cancer diagnosis and treatment. In this study, experiments were conducted to optimize the growth of Rhodobacter sphaeroides and production of ALA through LED irradiation of various wavelengths, addition of organic acid precursors of ALA, and changes in glucose concentration. After 72 h cultivation, the 850 nm wavelength LED irradiated at the same light intensity as the incandescent lamp increased the growth of R. sphaeroides and the production of ALA about 1.5- and 1.8-fold as compared with the control, respectively (p <0.0001 and p <0.0001). As a result of culturing R. sphaeroides by irradiating an LED with a wavelength of 850 nm after adding organic acid to the final concentration of 5 mM in culture medium, the production of ALA was increased about 2.8-fold in medium supplemented with pyruvic acid compared with the control (p <0.0001). In addition, the growth of the strain and the production of ALA were increased about 2.9- and 3.4-fold in medium supplemented with 40 mM glucose compared to the control which added only 5 mM pyruvic acid, respectively (p <0.0001 and p <0.0001). The yield of ALA per cell dry mass was about 1.4 folds higher than that of the control in 20 and 40 mM glucose, respectively (p <0.001). In conclusion, the growth of R. sphaeroides and production of ALA were increased by 850 nm wavelength LED irradiation. It also optimized the growth of R. sphaeroides and production of ALA through organic acid addition and glucose concentration changes.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.6
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• pp.800-807
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• 2019

Objective: The aim was to evaluate backgrounding beef steers on oat + ryegrass pastures mixed with vetch and/or using energy supplementation. Methods: A randomized block design with three treatments and three replications was used. The treatments were: grass + supplement (oat + ryegrass + supplementation), legume + supplement (oat + ryegrass + vetch + supplementation) and grass + legume (oat + ryegrass + vetch). A continuous grazing system with a variable stocking rate was used. Twenty-seven intact crossbred steers (1/4 Marchigiana, 1/4 Aberdeen Angus and 2/4 Nellore) aged 7 months old and average weight of 190 kg were used. Steers were supplemented at 1% of the body weight of ground corn. The experiment lasted 84 days, between May and August 2014. Behavioral assessments were performed two times per experimental period, for 24 hours. Results: The forage mass was different between treatments, being greater for steers fed without legume. The accumulation rate, forage allowance, and stocking rate did not differ between treatments due to the adequate adjustment of forage allowance. The final weight of animals, as well as the dry matter intake (kg/d), did not differ between treatments. However, forage intake was higher for non-supplemented animals in relation to supplemented steers. Supplement intake did not alter the total digestible nutrient intake due to pasture quality. Animals fed grass + supplement had higher live weight gain per area than those fed grass + legume. Animals without supplementation spent more time in grazing. Conclusion: Feeding behavior was not altered by mixing with vetch or supplementation. Non-supplemented animals started the grazing peak earlier and spent more time in grazing than those supplemented; however, the average daily gain was similar between treatments. The live weight gain per hectare was 47% higher in pastures in which the animals received supplementation compared with those mixed with vetch, a consequence of the substitutive effect.

• Tuberculosis and Respiratory Diseases
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• v.84 no.1
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• pp.46-54
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• 2021

Background: The aim of this study was to investigate inhaler device handling in elderly patients. Inhaler devices with respect to misuse and error correction were also compared. Methods: Inhaler use technique was assessed using standardized checklists at the first visit and 3-month follow-up visit after retraining. The primary outcome was difference in the acceptable use ratio among inhaler devices. Secondary outcomes included differences in error correction, the most common step of misuse, and factors affecting the accuracy of inhaler use. Results: A total of 251 patients (mean age, 76.4 years) were included. The handling of 320 devices was assessed in the study. All patients had been trained before. However, only 24.7% of them used inhalers correctly. Proportions of acceptable use for Evohaler, Respimat, Turbuhaler, Ellipta, and Breezhaler/Handihaler were 38.7%, 50.0%, 61.4%, 60.8%, and 43.2%, respectively (p=0.026). At the second visit, the acceptable use ratio had increased. There were no significant differences among inhaler types (Evohaler, 63.9%; Respimat, 86.1%; Turbuhaler, 74.3%; Ellipta, 64.6%; and Breezhaler/Handihaler, 65.3% [p=0.129]). In multivariate analysis, body mass index, Turbuhaler, and Ellipta showed positive correlations with acceptable use of inhalers, whereas Chronic Obstructive Pulmonary Disease Assessment Test score showed a negative correlation. Conclusion: Although new inhalers have been developed, the accuracy of inhaler use remains low. Elderly patients showed more errors when using pressurized metered-dose inhalers than using dry powder inhalers and soft-mist inhalers. However, there were no significant differences in misuse among inhaler devices after individual training. Results of this study suggests that repeat training is more important than inhaler type.

• The Korean Journal of Food And Nutrition
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• v.33 no.6
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• pp.655-665
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• 2020

In this study, chemical information on a total of 20 individual compounds was constructed to identify isoflavones from the previous reports related with used parts(seeds, leaves, stems, pods) and products of soybean(Glycine max L.). Through constructed library and UPLC-DAD-QToF/MS analysis, a total of 19 individual isoflavones including aglycones, glucosides, acetylglucosides and malonylglucosides as major compounds was identified and quantified from 14 selected soybean seeds. Among them, genistein 7-O-(2"-O-apiosyl)glucoside and genistein 7-O-(6"-O-apiosyl)glucoside(ambocin) were identified tentatively as novel compounds in soybean seeds. Besides, among malonylglucosides, glycitein 4'-O-(6"-O-malonyl)glucoside was estimated for the first time. Total isoflavone contents were distributed from 240.21 to 445.21(mg/100 g, dry matter) and 7-O-6"-O-malonylglucosides were composed of 77.8% on total isoflavone as well as genistein derivatives were confirmed as major class. It was considered importantly that the development of isoflavone-rich varieties was necessary to strengthen their effects such as anti-inflammation, anti-cancer and menopause mitigation. The qualitative and quantitative data presented precisely in this study could be help to select and breed isoflavone-rich varieties. Furthermore, their basic isoflavone profile is expected to be applied to estimate the change of isoflavone conjugates on bioavailability after soy food supplements.

• Journal of Microbiology and Biotechnology
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• v.32 no.5
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• pp.630-637
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• 2022

The objective of this study was to optimize industrial-grade media for improving the biomass production of Weissella cibaria JW15 (JW15) using a statistical approach. Eleven variables comprising three carbon sources (glucose, fructose, and sucrose), three nitrogen sources (protease peptone, yeast extract, and soy peptone), and five mineral sources (K₂HPO₄, potassium citrate, ⳑ-cysteine phosphate, MgSO₄, and MnSO₄) were screened by using the Plackett-Burman design. Consequently, glucose, sucrose, and soy peptone were used as significant variables in response surface methodology (RSM). The composition of the optimal medium (OM) was 22.35 g/l glucose, 15.57 g/l sucrose, and 10.05 g/l soy peptone, 2.0 g/l K₂HPO₄, 5.0 g/l sodium acetate, 0.1 g/l MgSO₄·7H₂O, 0.05 g/l MnSO₄·H₂O, and 1.0 g/l Tween 80. The OM significantly improved the biomass production of JW15 over an established commercial medium (MRS). After fermenting OM, the dry cell weight of JW15 was 4.89 g/l, which was comparable to the predicted value (4.77 g/l), and 1.67 times higher than that of the MRS medium (3.02 g/l). Correspondingly, JW15 showed a rapid and increased production of lactic and acetic acid in the OM. To perform a scale-up validation, batch fermentation was executed in a 5-l bioreactor at 37℃ with or without a pH control at 6.0 ± 0.1. The biomass production of JW15 significantly improved (1.98 times higher) under the pH control, and the cost of OM was reduced by two-thirds compared to that in the MRS medium. In conclusion, OM may be utilized for mass producing JW15 for industrial use.