The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Characterization of Isoflavones from Seed of Selected Soybean (Glycine max L.) Resources Using High-Resolution Mass Spectrometry

고해상도 질량 분석을 이용한 대두(Glycine max L.) 우수자원 종자의 이소플라본 특성 평가

  • Lee, So-Jeong (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Heon-Woong (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Suji (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Na, Hyemin (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Kwon, Ryeong Ha (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Kim, Ju Hyung (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Yoon, Hyemyeong (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Choi, Yu-Mi (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Wee, Chi-Do (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Yoo, Seon Mi (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA) ;
  • Lee, Sang Hoon (Dept. of Agro-Food Resources, National Institute of Agricultural Sciences, RDA)
  • 이소정 (국립농업과학원 농식품자원부) ;
  • 김헌웅 (국립농업과학원 농식품자원부) ;
  • 이수지 (국립농업과학원 농식품자원부) ;
  • 라혜민 (국립농업과학원 농식품자원부) ;
  • 권령하 (국립농업과학원 농식품자원부) ;
  • 김주형 (국립농업과학원 농식품자원부) ;
  • 윤혜명 (국립농업과학원 농업유전자원센터) ;
  • 최유미 (국립농업과학원 농업유전자원센터) ;
  • 위치도 (국립농업과학원 농식품자원부) ;
  • 유선미 (국립농업과학원 농식품자원부) ;
  • 이상훈 (국립농업과학원 농식품자원부)
  • Received : 2020.11.05
  • Accepted : 2020.11.24
  • Published : 2020.12.31
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Abstract

In this study, chemical information on a total of 20 individual compounds was constructed to identify isoflavones from the previous reports related with used parts(seeds, leaves, stems, pods) and products of soybean(Glycine max L.). Through constructed library and UPLC-DAD-QToF/MS analysis, a total of 19 individual isoflavones including aglycones, glucosides, acetylglucosides and malonylglucosides as major compounds was identified and quantified from 14 selected soybean seeds. Among them, genistein 7-O-(2"-O-apiosyl)glucoside and genistein 7-O-(6"-O-apiosyl)glucoside(ambocin) were identified tentatively as novel compounds in soybean seeds. Besides, among malonylglucosides, glycitein 4'-O-(6"-O-malonyl)glucoside was estimated for the first time. Total isoflavone contents were distributed from 240.21 to 445.21(mg/100 g, dry matter) and 7-O-6"-O-malonylglucosides were composed of 77.8% on total isoflavone as well as genistein derivatives were confirmed as major class. It was considered importantly that the development of isoflavone-rich varieties was necessary to strengthen their effects such as anti-inflammation, anti-cancer and menopause mitigation. The qualitative and quantitative data presented precisely in this study could be help to select and breed isoflavone-rich varieties. Furthermore, their basic isoflavone profile is expected to be applied to estimate the change of isoflavone conjugates on bioavailability after soy food supplements.

Keywords

Acknowledgement

본 연구는 농촌진흥청의 국가연구개발사업(과제번호: PJ014172012020), 전문연구원 및 학·연협동과정 지원사업에 의해 수행한 결과의 일부이며, 지원에 감사드립니다.

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