• 한국육종학회지
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• 제40권4호
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• pp.361-370
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• 2008

In order to better understand the morphological differentiation of the two cultivated types of Perilla crop and their weedy types in Korea and Japan, we studied the variation of 62 accessions by examining 15 morphological characteristics. By using ANOVA (one-way analysis of variance), we determined that var. frutescens and var. crispa showed significant morphological differences in terms of plant height and seed weight. Furthermore, cultivated var. frutescens and var. crispa could also be clearly discriminated from one another using PCA (principal component analysis). Specifically, quantitative and qualitative characteristics such as plant height, seed weight, degree of pubescence, shape of leaf, color of leaf, fragrance of plant, color of flower, color of stem and seed size greatly contributed to differences seen in the positive and negative direction on the first axis. In our study, most accessions of cultivated var. frutescens and those of its weedy type could be clearly discriminated from one another, however, most accessions of cultivated and weedy types of var. crispa were not clearly discriminated by the ANOVA and PCA analyses. These results indicated that cultivated var. frutescens can be considered to be a domesticated form, while the cultivated var. crispa can not be considered to be a domesticated form in Korea and Japan. It is our belief that our results concerning the morphological variations among cultivated types of Perilla crop and their weedy types in Korea and Japan will help ensure the long-term success of breeding programs and maximize the use of the germplasm resources in Korea.

• Fisheries and Aquatic Sciences
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• 제21권11호
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• pp.34.1-34.13
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• 2018

Sexual maturity ($L_{50}$), the length at which 50% of fish in a size class are mature, is a key aspect of domestication of new fish species because it guides the procedure for identification of appropriate broodstock size for artificial spawning. In this study, the $L_{50}$ was determined for 1083 Barbus altianalis samples obtained from Lake Edward and the Upper Victoria Nile. Gonads of freshly killed samples were examined macroscopically and verified with standard histological procedures for the maturation stages that were used to determine $L_{50}$. Oocytes and spermatogenic cell sizes were compared for fish obtained from both water bodies. Results indicated that there were no variations in macro gonad features observed for fish from Lake Edward and Upper Victoria Nile. Similarly, there were no significant differences in oocyte sizes (P > 0.05) between the two populations but significant differences in spermatogenic cell sizes were noted (P < 0.05) except for spermatozoa (P > 0.05). This however did not suggest peculiar differences between the two populations for staging the gonads. Consequently, no staging variations were suggested for both populations in determination of $L_{50}$. Sexual maturity was found in the same class size of fork length (FL) 20-24.9 cm and 35-39.9 cm for males and females from both water bodies, respectively. At this FL, however, males were too small, and for good selection of vigor broodstocks for spawning and conservation purposes, they are better picked from class size of 30-34.9 cm FL and above. These findings were crucial for integration of appropriate breeding size in spawning protocol by farmers and fisheries scientists conserving wild B. altianalis populations.

• Journal of Forest and Environmental Science
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• 제37권2호
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• pp.104-115
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• 2021

The research attempted to analyze the medicinal plant species richness in the vertical gradient from lower to the highest elevation of Makawanpur, focusing on the relationship between species richness and elevation which is very important for conservation and management of species diversity. Inventory was carried out in the study area by taking sampling intensity of 0.5% in the effective area. Altogether, 42 sample plots were laid in the field with the help of GIS software maintaining 50 m altitude difference. High species diversity was found in the herbs species whereas shrubs have comparatively low species diversity. The maximum species richness is found in herbs and poles whereas shrubs and trees have relatively low species richness. Research showed that species richness of medicinal plants increased with altitudinal gradient. While analyzing the species richness from 350 to 2,550 m (msl), the highest species richness was received with the elevation ranges from 1,800 m to 2,300 m. There was a positive relationship between species richness and altitudinal gradient in the study area. In addition, we have recorded the high value medicinal plants after 1,800 m altitude and rarely within 1,000 m. Medicinal plants correlated both positive and negative relationships with the increased altitude. The altitudinal response has positively seen except density (n/ha) of Shrubs. Domestication and cultivation of high value medicinal plants should be promoted in community forest including private lands. Training, workshops and awareness programs should be conducted to make people aware about medicinal plants resource utilization, conservation and commercialization of available medicinal plants.

• Journal of Animal Science and Technology
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• 제63권2호
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• pp.248-261
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• 2021

Attributable to their major function in pathogen recognition, the use of bovine leukocyte antigens (BoLA) as disease markers in immunological traits in cattle is well established. However, limited report exists on polymorphism of the BoLA gene in zebu cattle breeds by high resolution typing methods. Thus, we used a polymerase chain reaction sequence-based typing (PCR-SBT) method to sequence exon 2 of the BoLA class II DRB3 gene from 100 animals (Boran, n = 13; Sheko, n = 20; Fogera, n = 16; Horro, n = 19), Hanwoo cattle (n = 18) and Bangladesh Red Chittagong zebu (n = 14). Out of the 59 detected alleles, 43 were already deposited under the Immuno Polymorphism Database for major histocompatibility complex (IPD-MHC) while 16 were unique to this study. Assessment of the level of genetic variability at the population and sequence levels with genetic distance in the breeds considered in this study showed that Zebu breeds had a gene diversity score greater than 0.752, nucleotide diversity score greater than 0.152, and mean number of pairwise differences higher than 14, being very comparable to those investigated for other cattle breeds. Regarding neutrality tests analyzed, we investigated that all the breeds except Hanwoo had an excess number of alleles and could be expected from a recent population expansion or genetic hitchhiking. Howbeit, the observed heterozygosity was not significantly (p < 0.05) higher than the expected heterozygosity. The Hardy Weinberg equilibrium (HWE) analysis revealed non-significant excess of heterozygote animals, indicative of plausible over-dominant selection. The pairwise FST values suggested a low genetic variation among all the breeds (FST = 0.056; p < 0.05), besides the rooting from the evolutionary or domestication history of the cattle. No detached clade was observed in the evolutionary divergence study of the BoLA-DRB3 gene, inferred from the phylogenetic tree based on the maximum likelihood model. The investigation herein indicated the clear differences in BoLA-DRB3 gene variability between African and Asian cattle breeds.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.219-219
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• 2022

Granule-bound starch synthase I (GBSSI), encoded by the waxy gene, is responsible for the accumulation of amylose during the development of starch granules in rice endosperm. Despite many findings on waxy alleles, the genetic diversity and evolutionary studies are still not fully explored regarding their functional effects. Comprehensive evolutionary analyses were performed to investigate the genetic variations and relatedness of the GBSSI gene in 374 rice accessions composed of 54 wild accessions and 320 bred cultivars (temperate japonica, tropical japonica, indica, aus, aromatic, and admixture). GBSS1 coding regions were analyzed from a VCF file retrieved from whole-genome resequencing data, and eight haplotypes were identified in the GBSSI coding region of 320 bred cultivars. The genetic diversity indices revealed the most negative Tajima's D value in the tropical-japonica, followed by the aus and temperate-japonica, while Tajima's D values in indica were positive, indicating balancing selection. Diversity reduction was noticed in temperate japonica (0.0003) compared to the highest one (wild, 0.0044), illustrating their higher genetic differentiation by F_ST-value (0.604). The most positive Tajima's D value was observed in indica (0.5224), indicating the GBSSI gene domestication signature under balancing selection. In contrast, the lowest and negative Tajima's D value was found in tropical japonica (-0.5291), which might have experienced a positive selection and purified due to the excess of rare alleles. Overall, our study offers insights into haplotype diversity and evolutionary fingerprints of GBSSI. It ako provides genomic information to increase the starch content of cooked rice.

• Journal of Animal Science and Technology
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• 제46권6호
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• pp.917-924
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• 2004

미토콘드리아 ND2 유전자와 세 가지 tRNA (tRNA-M앙, tRNA-Trp, tRNA-Ala)들이 포함}는 mtDNA 절펀의 PCR-RFLP haplotyping 기법으로 제주도에서 사육하는 한국 재래돼지를 포함하는 5개 품종에 대한 유전적 다양성을 조사하였다. 몇몇 돼지 품종에서 mtDNA 상의 제한절편 길이의 다형성이 관찰되었다. HaeIll-와 Hinf1RFLP에서는 두 가지 제한절편 유형, Tsp509IRFLP에서는 네 가지 유형으로 각각 구분되었다. 발견된 제한절편 유형들을 조협하여 mtDNA haplotype으로 구분했을 때, 모두 네 가지 haplotype들이 발견되었고 집단에 따라 각기 다른빈도를 나타내였다. 하나의 동일한 haplotype mtWB가 한반도 야생멧돼지와 Large White 집단에서 관찰되었다. Duroc과 Landrace 품종들은 여러 모계 선조에서 유래되었을 것으로 추정되는 두 가지의 haplotype들을 가지고 있었다. 제주도에서 사육되고 있는 한국재래돼지 집단에서는 muD와 mtJN haplonpe들이 관찰되었는데, 이 중 mtJN은 빈도가 높고 집단 특이적이었다. 본 연구의 결과는 제주 돼지 집단의 형성에 적어도 둘 이상의 모계 선조가 참여했으며, 이후 동아시아 언근 돼지 품종들과의 인위적인 교잡아 이루어졌을 것으로 추정된다. 연구진의 예상과는 달리 한반도 야생멧돼지가 제주 재래돼지 집단의 모계 선조라는 증거는 확인되지 않았다. MtDNA haplotype과 돼지 계통간의 관계 에서의 특이성은 돼지 육종에 있어 모계 확인과 계보 구성에 매우 유용한 정보를 제공할 것으로 사료된다.

• 한국초지조사료학회지
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• 제13권2호
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• pp.86-92
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• 1993

The variation of the esterase isozyme, germination rate, chemical composition and digestibility of field bean(G1ycine soja S. and Z.) were estimated. The results are as follows; 1. The banding patterns of the esterase isozyme in field bean were varied with the tissue and habitat. 2. The enzyme activity of the Est-I, Est-2, Est-3 and Est-4 in field bean showed a high value compared with the other enzyme. 3. The range of germination temperature in field bean was 10-40C and the optimum germination temperature was 25- 38^{\circ}C.$. 4. The crude protein(CP) contents was 19.9% in the whole plant, 27.8% in the leaf and 45.9% in the seed, the cellulose contents was 29.5% in the whole plant, 23.0% in the leaf and 13.8% in the seed, the neutral detergent fiber(NDF) was 62.6% in the whole plant, 47.9% in the leaf and 47.9% in the seed and the acid detergent fiber(ADF) was 44.5% in the whole plant, 28.4% in the leaf and 28.4% in the seed, respectively. 5. The digestibility of the field bean was 44.1% in the whole plant, 49.6% in the leaf and 75.1% in the seed, NDF was 26.2% in the whole plant 46.2% in the leaf, ADF was 29.0% in the whole plant, 47.7% in the leaf and 58.0% in the seed and Cellulose was 48.7% in the whole plant, 58.0% in the leaf and 70.2% in the seed, respectively. 6. Total digestible nutrients(TDN) of the field bean was 47.4% in the whole plant, 51.5% in the leaf and 70.2% in the seed, respectively. The digestible energy(DE) value was 2.1 kcal/g in the whole plant, 2.27 kcal/g in the leaf and 3.10 kcal/g in the seed and the metabolizable energy(ME) value was 1.72 kcal/g in the whole plant, 1.86 kcal/g in the leaf and 3.23 kcal/g in the seed, respectively.

• Journal of Animal Science and Technology
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• 제47권3호
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• pp.341-354
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• 2005

For the genetic assessment of the cattle breeds including Hanwoo, eleven microsatellite markers on ten bovine autosomes were genetically characterized for 618 individuals of nineteen cattle breeds; North Eastern Asian breeds (Korean cattle, Korean Black cattle, Japanese Black cattle, Japanese Brown cattle, Yanbian cattle), Chinese yellow cattle (Luxi cattle, Nanyang cattle), European Bas taurus (Angus, Hereford, Charolais, Holstein, Limousin), African Bas taurus (N'Dama, Baoule), African Bas indicus (Kavirondo Zebu, White Fulani), Asian Bas indicus (Sahiwal, Nelore) and one Bali cattle, Bas banteng as an outbreed-reference population. Allele frequencies derived from the genotyping data were used in estimating heterozygosities, gene diversities and genetic distances. The microsatellite loci were highly polymorphic, with a total of 162 different alleles observed across all loci. Variability in allele numbers and frequencies was observed among the breeds. The average expected heterozygosity of North Eastern Asian breeds was higher than those of European and African taurines, but lower than those of Asian and African indicines. Genetic distances were estimated using Nei's DA genetic distance and the resultant DA matrix was used in the construction of the phylogenetic trees. The genetic distances between North Eastern Asian cattle breeds and Bas indicus were similar with those between European Bas taurus and Bas indicus, and African Bas taurus and Bas indicus, respectively. The clusters were clearly classified into North Eastern Asian, European and African taurines groups as well as different cluster with Chinese mainland breeds, firstly out-grouping with Bas indicus. These results suggest that Korean cattle, Hanwoo, had not been originated from a crossbred between Bas primigenius in Europe and Bas indicus in India and North Eastern Asian Bas taurus may be have separate domestication from European and African Bas taurus.

• 한국조경학회지
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• 제36권4호
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• pp.15-26
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• 2008

본 연구의 목적은 시간의 흐름에 따라 생성되는 사건을 매개체로 하여 골프코스를 해석하고자 하는 것이다. 골프경기를 하면서 티샷, 두 번째 샷, 피팅, 홀아웃과 같은 다양한 사건이 발생하는데, 이러한 사건들을 바탕으로 버디, 파, 보기, 더블보기와 같은 준원인에 의한 사건의 계열화가 발생한다. 그러나 이러한 사건의 계열화는 항상 예측할 수 없는 방향으로 진행된다. 이러한 예측 불가능한 사건의 발생은 공간의 특성을 변화시키는 동시에 골퍼에게는 잊을 수 없는 체험을 제공한다. 질 들뢰즈는 공간의 특성을 홈 패인 공간과 매끈한 공간으로 구분하고 있다. 홈 패인 공간이란 차원적이고 계산되어지고 중심을 가지는 원거리적 공간으로 정주민적 공간의 특성을 지니는 공간이며, 매끈한 공간은 방향적이고 탈중심적이며 촉지적인 근거리적 공간으로 유목민적 공간의 특성을 지니는 공간을 말한다. 본 연구는 이러한 매끈한 공간과 홈 패인 공간을 개념적 기준으로 하여 공간의 특성을 분석하였다. 골프코스는 본래 인공적으로 설계된 매끈한 공간의 특성을 지니고 있으나, 골프경기가 진행되면서 사건생성적인 공간으로 변모한다. 즉, 동적이고 변화무쌍하며, 스케일이 장소에 따라 가변화되는 매끈한 공간으로 탈바꿈하게 된다. 경기를 진행함에 따라 이러한 공간적 특성의 반전은 예측할 수 없는 다양한 사건을 통하여 반복된다. 또한, 경기의 주체인 골퍼는 정위-중심설정-한정-순치라는 일련의 장소만들기 과정을 통하여 골프코스와의 역동적, 유기적 맞물림을 통하여 현상학적 체험과정을 느끼게 된다.

• 식물병연구
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• 제14권3호
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• pp.201-204
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• 2008

약용작물인 짚신나물에 발생하는 흰가루병의 병원균은 Sphaerotheca aphanis이고, 장마 전 건조기인 $5{\sim}6$월과 8월 말에서 9월까지 2차례 발병하였다. 흰가루병은 잎, 줄기 및 종자 모두에서 관찰 되었다. 난황유 처리에 의한 흰가루병 방제효과는 1회 처리구의 방제가가 26.9%에 불과하였으나, 2회 및 3회 처리구에서는 80.8%, 82.7%의 방제가를 나타내었다. 처리회수에 관계없이 처리 직후에는 흰가루병의 방제 효과가 뚜렷하였으나 1회 처리구에는 처리 7일 후에는 발병이 다시 증가하였던 반면, 2회 및 3회 처리구에는 처리 후 28일까지도 발병이 억제 되는 경향을 보였다. 따라서 짚신나물 흰가루병은 난황유를 7일 간격으로 2회 처리하는 것이 바람직한 방제 방법이라 생각된다.